TY - CHAP AU - Hajna, Zsófia Réka AU - Csekő, Kata AU - Kemény, Ágnes AU - Kereskai, László AU - Tamás, Kiss AU - Anikó, Perkecz AU - Szitter, István AU - Kocsis, Béla AU - Pintér, Erika AU - Helyes, Zsuzsanna ED - Rakonczay, Zoltán ED - Kiss, Lóránd TI - Complex regulatory role of the TRPA1 receptor in acute and chronic airway inflammation mouse models T2 - Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project PB - University of Szeged CY - Szeged SN - 9789633067642 PY - 2020 SP - 58 EP - 58 PG - 1 UR - https://m2.mtmt.hu/api/publication/31684069 ID - 31684069 LA - English DB - MTMT ER - TY - JOUR AU - Hajna, Zsófia Réka AU - Csekő, Kata AU - Kemény, Ágnes AU - Kereskai, László AU - Kiss, Tamás AU - Perkecz, Anikó AU - Szitter, István AU - Kocsis, Béla AU - Pintér, Erika AU - Helyes, Zsuzsanna TI - Complex Regulatory Role of the TRPA1 Receptor in Acute and Chronic Airway Inflammation Mouse Models JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 11 PG - 21 SN - 1661-6596 DO - 10.3390/ijms21114109 UR - https://m2.mtmt.hu/api/publication/31345123 ID - 31345123 N1 - * Megosztott szerzőség AB - The Transient Receptor Potential Ankyrin 1 (TRPA1) cation channel expressed on capsaicin-sensitive afferents, immune and endothelial cells is activated by inflammatory mediators and exogenous irritants, e.g., endotoxins, nicotine, crotonaldehyde and acrolein. We investigated its involvement in acute and chronic pulmonary inflammation using Trpa1 gene-deleted (Trpa1-/-) mice. Acute pneumonitis was evoked by intranasal Escherichia coli endotoxin (lipopolysaccharide: LPS) administration, chronic bronchitis by daily cigarette smoke exposure (CSE) for 4 months. Frequency, peak inspiratory/expiratory flows, minute ventilation determined by unrestrained whole-body plethysmography were significantly greater, while tidal volume, inspiratory/expiratory/relaxation times were smaller in Trpa1-/- mice. LPS-induced bronchial hyperreactivity, myeloperoxidase activity, frequency-decrease were significantly greater in Trpa1-/- mice. CSE significantly decreased tidal volume, minute ventilation, peak inspiratory/expiratory flows in wildtypes, but not in Trpa1-/- mice. CSE remarkably increased the mean linear intercept (histopathology), as an emphysema indicator after 2 months in wildtypes, but only after 4 months in Trpa1-/- mice. Semiquantitative histopathological scores were not different between strains in either models. TRPA1 has a complex role in basal airway function regulation and inflammatory mechanisms. It protects against LPS-induced acute pneumonitis and hyperresponsiveness, but is required for CSE-evoked emphysema and respiratory deterioration. Further research is needed to determine TRPA1 as a potential pharmacological target in the lung. LA - English DB - MTMT ER - TY - JOUR AU - Tékus, Éva AU - Mikó, Alexandra AU - Füredi, Nóra AU - Rostás, Ildikó AU - Tenk, Judit AU - Kiss, T AU - Szitter, István AU - Balaskó, Márta AU - Helyes, Zsuzsanna AU - Wilhelm, Márta AU - Pétervári, Erika TI - Body fat of rats of different age-groups and nutritional states: assessment by micro-CT and skinfold thickness. JF - JOURNAL OF APPLIED PHYSIOLOGY J2 - J APPL PHYSIOL VL - 124 PY - 2018 IS - 2 SP - 268 EP - 275 PG - 8 SN - 8750-7587 DO - 10.1152/japplphysiol.00884.2016 UR - https://m2.mtmt.hu/api/publication/3250998 ID - 3250998 N1 - * Megosztott szerzőség AB - Obesity presents a growing public health problem. Therefore, the analysis of body composition is important in clinical practice as well as in animal research models of obesity, hence precise methods for the assessment of body fat would be essential. We aimed to evaluate in vivo abdominal micro-computed tomography scan restricted to the L1-L3 region (micro-CT(L1-L3)), a skinfold thickness-based method (STM) and post mortem body composition analysis (PMA) with regard to whole-body micro-CT scan in rats. Male Wistar rats of different age-groups (from 3 to 24 months) and nutritional states (normally fed, high-fat diet-induced obese, calorie-restricted) were used. The fat percentage was determined with micro-CT(L1-L3) and whole-body scan in anesthesized rats. Their skinfold thickness was measured in five locations with Lange caliper. Wet weights of epididymal and retroperitoneal fat pads were determined via PMA. With regard to fat mass, the strongest correlation was observed between abdominal and whole-body micro-CT. The other methods showed weaker associations with whole-body micro-CT and with each other. Micro-CT(L1-L3) and PMA showed similar age-associated increase in fat mass between 3-18 months. Micro-CT(L1-L3), STM and PMA were efficient to detect differences in fat mass values in groups of different nutritional states. Micro-CT(L1-L3) appears to be a useful method for body fat assessment in rats with reduced scanning time. In rats STM may also be a useful, low-priced, non-invasive and simple in vivo technique to assess obesity. LA - English DB - MTMT ER - TY - JOUR AU - Kemény, Ágnes AU - Csekő, Kata AU - Szitter, István AU - Varga, Zoltán AU - Bencsik, Péter AU - Kiss, Krisztina AU - Halmosi, Róbert AU - Deres, László AU - Erős, Krisztián AU - Perkecz, A AU - Kereskai, László AU - Horváthné László, Terézia AU - Kiss, T AU - Ferdinandy, Péter AU - Helyes, Zsuzsanna TI - Integrative characterization of chronic cigarette smoke-induced cardiopulmonary comorbidities in a mouse model JF - ENVIRONMENTAL POLLUTION J2 - ENVIRON POLLUT VL - 229 PY - 2017 SP - 746 EP - 759 PG - 14 SN - 0269-7491 DO - 10.1016/j.envpol.2017.04.098 UR - https://m2.mtmt.hu/api/publication/3244396 ID - 3244396 N1 - * Megosztott szerzőség AB - Cigarette smoke-triggered inflammatory cascades and consequent tissue damage are the main causes of chronic obstructive pulmonary disease (COPD). There is no effective therapy and the key mediators of COPD are not identified due to the lack of translational animal models with complex characterization. This integrative chronic study investigated cardiopulmonary pathophysiological alterations and mechanisms with functional, morphological and biochemical techniques in a 6-month-long cigarette smoke exposure mouse model. Some respiratory alterations characteristic of emphysema (decreased airway resistance: Rl; end-expiratory work and pause: EEW, EEP; expiration time: Te; increased tidal mid-expiratory flow: EF50) were detected in anaesthetized C57BL/6 mice, unrestrained plethysmography did not show changes. Typical histopathological signs were peribronchial/perivascular (PB/PV) edema at month 1, neutrophil/macrophage infiltration at month 2, interstitial leukocyte accumulation at months 3-4, and emphysema/atelectasis at months 5-6 quantified by mean linear intercept measurement. Emphysema was proven by micro-CT quantification. Leukocyte number in the bronchoalveolar lavage at month 2 and lung matrix metalloproteinases-2 and 9 (MMP-2/MMP-9) activities in months 5-6 significantly increased. Smoking triggered complex cytokine profile change in the lung with one characteristic inflammatory peak of C5a, interleukin-1alpha and its receptor antagonist (IL-1alpha, IL-1ra), monokine induced by gamma interferon (MIG), macrophage colony-stimulating factor (M-CSF), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) at months 2-3, and another peak of interferon-gamma (IFN-gamma), IL-4, 7, 13, 17, 27 related to tissue destruction. Transient systolic and diastolic ventricular dysfunction developed after 1-2 months shown by significantly decreased ejection fraction (EF%) and deceleration time, respectively. These parameters together with the tricuspid annular plane systolic excursion (TAPSE) decreased again after 5-6 months. Soluble intercellular adhesion molecule-1 (sICAM-1) significantly increased in the heart homogenates at month 6, while other inflammatory cytokines were undetectable. This is the first study demonstrating smoking duration-dependent, complex cardiopulmonary alterations characteristic to COPD, in which inflammatory cytokine cascades and MMP-2/9 might be responsible for pulmonary destruction and sICAM-1 for heart dysfunction. LA - English DB - MTMT ER - TY - THES AU - Szitter, István TI - A tachykinin rendszer és az érzőideg-végződések aktivációjának szerepe bél-és légúti gyulladásmodellekben PB - Pécsi Tudományegyetem PY - 2014 SP - 99 UR - https://m2.mtmt.hu/api/publication/3007813 ID - 3007813 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kun, József AU - Szitter, István AU - Kemény, Ágnes AU - Perkecz, A AU - Kereskai, László AU - Pohóczky, Krisztina AU - Vincze, Áron AU - Gódi, Szilárd AU - Szabó, Imre AU - Szolcsányi, János AU - Pintér, Erika AU - Helyes, Zsuzsanna TI - Upregulation of the transient receptor potential ankyrin 1 ion channel in the inflamed human and mouse colon and its protective roles. JF - PLOS ONE J2 - PLOS ONE VL - 9 PY - 2014 IS - 9 PG - 15 SN - 1932-6203 DO - 10.1371/journal.pone.0108164 UR - https://m2.mtmt.hu/api/publication/2750029 ID - 2750029 N1 - Megosztott utolsó szerzőség Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Pécs, Hungary Molecular Pharmacology Research Group, János Szentágothai Research Center, University of Pécs, Pécs, Hungary Department of Pathology, Medical School, University of Pécs, Pécs, Hungary 1st Department of Internal Medicine, University of Pécs, Pécs, Hungary Cited By :63 Export Date: 9 December 2020 CODEN: POLNC Correspondence Address: Helyes, Z.; Department of Pharmacology and Pharmacotherapy, Medical School, University of PécsHungary Funding Agency and Grant Number: Social Renewal Operational Programme (SROP) [4.2.2.A-11/1/KONV-2012-0024, 4.2.4.A/2-11-1-2012-0001]; National Development Agency - Government of Hungary; European UnionEuropean Union (EU); European Social FundEuropean Social Fund (ESF); Office of Justice and Public Administration Funding text: The research was supported by the Social Renewal Operational Programme (SROP)-4.2.2.A-11/1/KONV-2012-0024. Funding agency: National Development Agency - Government of Hungary. http://palyazat.gov.hu/. E. Pinter and J. Kun were supported by scholarships of the Social Renewal Operational Programme (SROP)-4.2.4.A/2-11-1-2012-0001 "National Excellence Program - Elaborating and operating an inland student and researcher personal support system convergence program.'' The project was subsidized by the European Union and co-financed by the European Social Fund. Funding agency: Office of Justice and Public Administration. http://kih.gov.hu/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Pécs, Hungary Molecular Pharmacology Research Group, János Szentágothai Research Center, University of Pécs, Pécs, Hungary Department of Pathology, Medical School, University of Pécs, Pécs, Hungary 1st Department of Internal Medicine, University of Pécs, Pécs, Hungary Cited By :68 Export Date: 10 June 2021 CODEN: POLNC Correspondence Address: Helyes, Z.; Department of Pharmacology and Pharmacotherapy, Medical School, University of PécsHungary Export Date: 16 September 2021 CODEN: POLNC Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Pécs, Hungary Molecular Pharmacology Research Group, János Szentágothai Research Center, University of Pécs, Pécs, Hungary Department of Pathology, Medical School, University of Pécs, Pécs, Hungary 1st Department of Internal Medicine, University of Pécs, Pécs, Hungary Cited By :72 Export Date: 17 September 2021 CODEN: POLNC Correspondence Address: Helyes, Z.; Department of Pharmacology and Pharmacotherapy, Medical School, University of PécsHungary Export Date: 20 September 2021 CODEN: POLNC AB - Transient Receptor Potential Ankyrin 1 (TRPA1) channels are localized on sensory nerves and several non-neural cells, but data on their functional significance are contradictory. We analysed the presence and alterations of TRPA1 in comparison with TRP Vanilloid 1 (TRPV1) at mRNA and protein levels in human and mouse intact and inflamed colons. The role of TRPA1 in a colitis model was investigated using gene-deficient mice. TRPA1 and TRPV1 expressions were investigated in human colon biopsies of healthy subjects and patients with inflammatory bowel diseases (IBD: ulcerative colitis, Crohn's disease) with quantitative PCR and immunohistochemistry. Mouse colitis was induced by oral 2% dextran-sulphate (DSS) for 10 days. For investigating the functions of TRPA1, Disease Activity Index (weight loss, stool consistency, blood content) was determined in C57BL/6-based Trpa1-deficient (knockout: KO) and wildtype (WT) mice. Sensory neuropeptides, their receptors, and inflammatory cytokines/chemokines were determined with qPCR or Luminex. In human and mouse colons TRPA1 and TRPV1 are located on epithelial cells, macrophages, enteric ganglia. Significant upregulation of TRPA1 mRNA was detected in inflamed samples. In Trpa1 KO mice, Disease Activity Index was significantly higher compared to WTs. It could be explained by the greater levels of substance P, neurokinins A and B, neurokinin 1 receptor, pituitary adenylate-cyclase activating polypeptide, vasoactive intestinal polypeptide, and also interleukin-1beta, macrophage chemoattractant protein-1, monokine induced by gamma interferon-1, tumor necrosis factor-alpha and B-lymphocyte chemoattractant in the distal colon. TRPA1 is upregulated in colitis and its activation exerts protective roles by decreasing the expressions of several proinflammatory neuropeptides, cytokines and chemokines. LA - English DB - MTMT ER - TY - JOUR AU - Sütő, Balázs AU - Szitter, István AU - Bagoly, T AU - Pintér, Erika AU - Szolcsányi, János AU - Loibl, Csaba AU - Németh, Timea AU - Tánczos, Krisztián AU - Molnár, Tihamér AU - Leiner, Tamás AU - Varnai, B AU - Bardonicsek, Zs AU - Helyes, Zsuzsanna TI - Plasma somatostatin-like immunoreactivity increases in the plasma of septic patients and rats with systemic inflammatory reaction: experimental evidence for its sensory origin and protective role JF - PEPTIDES J2 - PEPTIDES VL - 54 PY - 2014 SP - 49 EP - 57 PG - 9 SN - 0196-9781 DO - 10.1016/j.peptides.2014.01.006 UR - https://m2.mtmt.hu/api/publication/2505156 ID - 2505156 LA - English DB - MTMT ER - TY - JOUR AU - Szitter, István AU - Pintér, Erika AU - Perkecz, A AU - Kemény, Ágnes AU - Kun, József AU - Kereskai, László AU - Pietra, C AU - Quinn, JP AU - Zimmer, A AU - Berger, A AU - Paige, CJ AU - Helyes, Zsuzsanna TI - Role of neurokinin 1 receptors in dextran sulfate-induced colitis: studies with gene-deleted mice and the selective receptor antagonist netupitant JF - INFLAMMATION RESEARCH J2 - INFLAMM RES VL - 63 PY - 2014 IS - 5 SP - 399 EP - 409 PG - 11 SN - 1023-3830 DO - 10.1007/s00011-014-0712-x UR - https://m2.mtmt.hu/api/publication/2505155 ID - 2505155 N1 - Department of Pharmacology and Pharmacotherapy, University of Pécs, Pécs, Hungary János Szentágothai Research Centre, Pécs, Hungary PharmInVivo Ltd., Pécs, Hungary Department of Pathology, University of Pécs, Pécs, Hungary Helsinn Healthcare SA, Preclinical R and D, Pambio-Noranco, Lugano, Switzerland Department of Molecular and Clinical Pharmacology, Institute of Translation, Liverpool University, Liverpool, United Kingdom Institute of Molecular Psychiatry, University of Bonn, Bonn, Germany Ontario Cancer Institute, University Health Network, Toronto, ON, Canada Department of Immunology, University of Toronto, Toronto, ON, Canada Cited By :10 Export Date: 19 September 2023 CODEN: INREF Correspondence Address: Helyes, Z.; Department of Pharmacology and Pharmacotherapy, University of Pécs, Pécs, Hungary; email: zsuzsanna.helyes@aok.pte.hu LA - English DB - MTMT ER - TY - GEN AU - Szitter, István AU - Barbara, K AU - Kiss, T AU - Perkecz, A AU - Feller, D AU - Helyes, Zs TI - Tüdő morfometria: dohányfüst okozta szöveti elváltozások kvantitatív követése mikroCT módszerrel C57BL/6 és galanin 3 receptor (Gal3r) deficiens egerekben PY - 2013 SP - A-0191 UR - https://m2.mtmt.hu/api/publication/2361697 ID - 2361697 N1 - [Poszter] LA - Hungarian DB - MTMT ER - TY - GEN AU - Kun, J AU - Helyes, Zs AU - Szitter, István AU - Gódi, Sz AU - Szabó, I AU - Kemény, Á AU - Pohóczky, Krisztina AU - Perkecz, A AU - Pintér, E TI - A tranziens receptor potenciál ankyrin 1 (TRPA1) és vanilloid 1 (TRPV1) receptor mRNS expresszió gyulladásos bélbetegségben (IBD) és dextrán-szulfát (DSS) által indukált egér colitis modellben PY - 2013 SP - A-0184 UR - https://m2.mtmt.hu/api/publication/2361693 ID - 2361693 N1 - [Poszter] LA - Hungarian DB - MTMT ER -