TY - CONF AU - Héninger, Réka AU - Mutuku, Christopher AU - Melegh, Szilvia Zsóka AU - Kovács, Krisztina AU - Urbán, Péter AU - Virág, Eszter Andrea AU - Herczeg, Róbert AU - Sonnevend, Ágnes AU - Gyenesei, Attila AU - Fekete, Csaba AU - Gazdag, Zoltán TI - Detection and molecular characterization of antibiotic resistant Enterobacteriaceae from hospital effluents and wastewater treatment plant T2 - CEESME Central and Eastern Europe Symposium on Microbial Ecology PB - International Society for Microbial Ecology C1 - Budapest PY - 2023 SP - 77 EP - 77 PG - 1 UR - https://m2.mtmt.hu/api/publication/34178794 ID - 34178794 N1 - [poster] LA - English DB - MTMT ER - TY - JOUR AU - Mutuku, Christopher AU - Kutasy, Barbara Júlia AU - Urbán, Péter AU - Melegh, Szilvia Zsóka AU - Herczeg, Róbert AU - Gazdag, Zoltán AU - Virág, Eszter Andrea TI - Plasmid sequence dataset of multidrug-resistant Enterobacterales isolated from hospital effluents and wastewater treatment plant JF - DATA IN BRIEF J2 - DATA BRIEF VL - 45 PY - 2022 PG - 9 SN - 2352-3409 DO - 10.1016/j.dib.2022.108736 UR - https://m2.mtmt.hu/api/publication/33258723 ID - 33258723 N1 - Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, 7622, Hungary Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences, Georgikon Campus Keszthely, Festetics Gy. u.7, Keszthely, 8360., Hungary Bioinformatics Research Group, Szentágothai Research Centre, Pécs, 7624, Hungary Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, 7622, Hungary Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Egyetem Square 1, Debrecen, 4032, Hungary Cited By :2 Export Date: 15 November 2023 Correspondence Address: Virág, E.; Department of Molecular Biotechnology and Microbiology, Egyetem Square 1, Hungary; email: virag.eszter@science.unideb.hu AB - We present plasmid sequences of 21 multidrug resistant isolates of Enterobacterales belonging to Escherichia coli (n=10), Klebsiella pneumoniae (n=9), Klebsiella oxytoca (n=1), and Citrobacter freundii (n=1). The isolates originated from effluent collected from hospital sewer pipes and from a wastewater treatment plant (WWTP) in a southwestern Hungarian city. Isolation was carried out using eosin methylene blue agar supplemented with ceftriaxone and the isolates were identified with MALDI-TOF MS. Screening for multidrug resistance was conducted by determining susceptibility to four chemical classes namely, beta-lactams, aminoglycoside, fluoroquinolone, and sulfonamide. Plasmid DNA was isolated by alkaline lysis method using the Monarch plasmid DNA miniprep kit from freshly grown pure colonies. Molecular typing and Illumina sequencing of plasmid DNA of multiresistant strains were performed. After the assembly of contigs, genes localized on plasmid sequences were determined and functionally annotated. These reconstructed plasmid sequences supplemented with gene functional annotations were deposited in the Mendeley data. Using these datasets different plasmid incompatibility groups were identified. These conjugative plasmids appear to play a key role in the transmission of multiple resistance genes in enteric bacteria via wastewater. The presented data may provide useful insight on the correlations between environmental antibiotic contamination and the development of bacterial resistance, which poses a serious public health threat. LA - English DB - MTMT ER - TY - JOUR AU - Told, Roland AU - Ujfalusi, Zoltán AU - Péntek, Attila AU - Kerényi, Mónika AU - Bánfai, Krisztina AU - Vizi, András AU - Szabó, Péter AU - Melegh, Szilvia Zsóka AU - Bovári-Biri, Judit AU - Pongrácz, Judit AU - Maróti, Péter TI - A state-of-the-art guide to the sterilization of thermoplastic polymers and resin materials used in the additive manufacturing of medical devices JF - MATERIALS AND DESIGN J2 - MATER DESIGN VL - 223 PY - 2022 PG - 17 SN - 0264-1275 DO - 10.1016/j.matdes.2022.111119 UR - https://m2.mtmt.hu/api/publication/33092180 ID - 33092180 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Mutuku, Christopher AU - Gazdag, Zoltán AU - Melegh, Szilvia Zsóka TI - Occurrence of antibiotics and bacterial resistance genes in wastewater : resistance mechanisms and antimicrobial resistance control approaches JF - WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY J2 - WORLD J MICROB BIOT VL - 38 PY - 2022 IS - 9 PG - 27 SN - 0959-3993 DO - 10.1007/s11274-022-03334-0 UR - https://m2.mtmt.hu/api/publication/32925509 ID - 32925509 AB - Antimicrobial pharmaceuticals are classified as emergent micropollutants of concern, implying that even at low concentrations, long-term exposure to the environment can have significant eco-toxicological effects. There is a lack of a standardized regulatory framework governing the permissible antibiotic content for monitoring environmental water quality standards. Therefore, indiscriminate discharge of antimicrobials at potentially active concentrations into urban wastewater treatment facilities is rampant. Antimicrobials may exert selective pressure on bacteria, leading to resistance development and eventual health consequences. The emergence of clinically important multiple antibiotic-resistant bacteria in untreated hospital effluents and wastewater treatment plants (WWTPs) has been linked to the continuous exposure of bacteria to antimicrobials. The levels of environmental exposure to antibiotics and their correlation to the evolution and spread of resistant bacteria need to be elucidated to help in the formulation of mitigation measures. This review explores frequently detected antimicrobials in wastewater and gives a comprehensive coverage of bacterial resistance mechanisms to different antibiotic classes through the expression of a wide variety of antibiotic resistance genes either inherent and/or exchanged among bacteria or acquired from the reservoir of antibiotic resistance genes (ARGs) in wastewater systems. To complement the removal of antibiotics and ARGs from WWTPs, upscaling the implementation of prospective interventions such as vaccines, phage therapy, and natural compounds as alternatives to widespread antibiotic use provides a multifaceted approach to minimize the spread of antimicrobial resistance. LA - English DB - MTMT ER - TY - JOUR AU - Mutuku, Christopher AU - Melegh, Szilvia Zsóka AU - Kovács, Krisztina AU - Urbán, Péter AU - Virág, Eszter Andrea AU - Héninger, Réka AU - Herczeg, Róbert AU - Sonnevend, Ágnes AU - Gyenesei, Attila AU - Fekete, Csaba AU - Gazdag, Zoltán TI - Characterization of β-Lactamases and Multidrug Resistance Mechanisms in Enterobacterales from Hospital Effluents and Wastewater Treatment Plant JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 11 PY - 2022 IS - 6 PG - 21 SN - 2079-6382 DO - 10.3390/antibiotics11060776 UR - https://m2.mtmt.hu/api/publication/32873680 ID - 32873680 LA - English DB - MTMT ER - TY - JOUR AU - Sonnevend, Ágnes AU - Alali, Walid Q. AU - Mahmoud, Sara A. AU - Ghazawi, Akela AU - Bharathan, Greeshma AU - Melegh, Szilvia Zsóka AU - Rizvi, Tahir A. AU - Pál, Tibor TI - Molecular Characterization of MCR-1 Producing Enterobacterales Isolated in Poultry Farms in the United Arab Emirates JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 11 PY - 2022 IS - 3 PG - 12 SN - 2079-6382 DO - 10.3390/antibiotics11030305 UR - https://m2.mtmt.hu/api/publication/32716546 ID - 32716546 N1 - Megosztott első szerzőség AB - Data on the prevalence of MCR-producing Enterobacterales of animal origin are scarce from the Arabian Peninsula. We investigated the presence and variety of such strains from fecal specimens of poultry collected in four farms in the United Arab Emirates. Colonies from ten composite samples per farm grown on colistin-supplemented plates were PCR-screened for alleles of the mcr gene. Thirty-nine isolates selected based on species, colony morphology, and plasmid profile were subjected to whole genome sequencing. The panel of their resistance and virulence genes, MLST and cgMLST were established. Transferability and incompatibility types of the MCR-plasmids were determined. mcr-1.1 positive strains were identified in 36 of the 40 samples. Thirty-four multi-drug resistant Escherichia coli of 16 different sequence types, two Escherichia albertii, two Klebsiella pneumoniae and one Salmonella minnesota were identified. Beyond various aminoglycoside, tetracycline, and co-trimoxazole resistance genes, seven of them also carried ESBL genes and one blaCMY-2. Six IncHI2, 26 IncI2 and 4 IncX4 MCR-plasmids were mobilized, in case of the IncHI2 plasmids co-transferring ampicillin, chloramphenicol and tetracycline resistance. The diversity of mcr-1 positive strains suggest a complex local epidemiology calling for a coordinated surveillance including animals, retail meat and clinical cases. LA - English DB - MTMT ER - TY - CHAP AU - Mutuku, Christopher AU - Kovács, Krisztina AU - Melegh, Szilvia Zsóka AU - Urbán, Péter AU - Sonnevend, Ágnes AU - Fekete, Csaba AU - Gazdag, Zoltán ED - Prisztóka, Gyöngyvér ED - Kertai, Bendegúz TI - Antimicrobial resistance and prevalence of extended spectrum and metallo-β-lactamases in Pseudomonas aeruginosa from clinical and municipal wastewater T2 - XIX. Szentágothai János Mutidiszciplináris Konferencia és Hallgatói Verseny: Absztrakt kötet = XIX. János Szentágothai Multidisciplinary Conference and Student Competition: Book of Abstracts PB - Szentágothai János Szakkollégium CY - Pécs SN - 9789634296478 PY - 2021 SP - 27 EP - 27 PG - 1 UR - https://m2.mtmt.hu/api/publication/32258096 ID - 32258096 LA - English DB - MTMT ER - TY - CHAP AU - Mutuku, Christopher AU - Kovács, Krisztina AU - Melegh, Szilvia Zsóka AU - Urbán, Péter AU - Sonnevend, Ágnes AU - Fekete, Csaba AU - Gazdag, Zoltán ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Mező, Emerencia TI - Detection of Vancomycin Resistant Enterococci (VRE) from Hospital Effluents T2 - Medical Conference for PhD Students and Experts of Clinical Sciences 2021 PB - University of Pécs, Doctoral Student Association CY - Pécs SN - 9789634296539 PY - 2021 SP - 107 EP - 107 PG - 1 UR - https://m2.mtmt.hu/api/publication/32131081 ID - 32131081 LA - English DB - MTMT ER - TY - CHAP AU - Mutuku, Christopher AU - Kovács, Krisztina AU - Melegh, Szilvia Zsóka AU - Héninger, Réka AU - Kulagin, D. AU - Urbán, Péter AU - Mestyan, Gyula. AU - Sonnevend, A. AU - Fekete, Csaba AU - Gazdag, Zoltán ED - Csiszár, Beáta ED - Hankó, Csilla ED - Kajos, Luca Fanni ED - Mező, Emerencia TI - Multiple Antimicrobial Resistance and Molecular Characterization of β-lactamases in enteric bacteria from Hospital Effluents and Wastewater Treatment Plant. T2 - Medical Conference for PhD Students and Experts of Clinical Sciences PB - University of Pécs, Doctoral Student Association CY - Pécs SN - 9789634295440 PY - 2020 SP - 70 EP - 70 PG - 1 UR - https://m2.mtmt.hu/api/publication/33547911 ID - 33547911 LA - English DB - MTMT ER - TY - JOUR AU - Gajdács, Márió AU - Németh, Anita AU - Knausz, Márta AU - Barrak, Ibrahim Ádám AU - Stájer, Anette AU - Mestyán, Gyula AU - Melegh, Szilvia Zsóka AU - Nyul, Adrienn AU - Tóth, Ákos AU - Ágoston, Zsuzsanna AU - Zsoldiné Urbán, Edit TI - Streptococcus suis: An Underestimated Emerging Pathogen in Hungary? JF - MICROORGANISMS J2 - MICROORGANISMS VL - 8 PY - 2020 IS - 9 PG - 18 SN - 2076-2607 DO - 10.3390/microorganisms8091292 UR - https://m2.mtmt.hu/api/publication/31408959 ID - 31408959 N1 - Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös utca 6., Szeged, 6720, Hungary Institute of Medical Microbiology, Faculty of Medicine, Semmelweis University, Nagyvárad tér 4., Budapest, 1089, Hungary Microbiology Laboratory, Petz Aladár County Teaching Hospital, Vasvári Pál utca 2–4., Győr, 9023, Hungary Department of Prosthodontics, Faculty of Dentistry, University of Szeged, Tiszta Lajos körút 62–64., Szeged, 6720, Hungary Department of Periodontology, Faculty of Dentistry, University of Szeged, Tiszta Lajos körút 62–64, Szeged, 6720, Hungary Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., Pécs, 7624, Hungary Department of Bacteriology, Mycology and Parasitology, National Public Health Center, Albert Flórián út 2–6., Budapest, 1097, Hungary Department of Anaesthesiology and Intensive Therapy, Faculty of Medicine, University of Szeged, Semmelweis utca 6., Szeged, 6725, Hungary Institute of Translational Medicine, Faculty of Medicine, University of Pécs, Szigeti út 12, Pécs, 7624, Hungary Cited By :14 Export Date: 7 September 2023 Correspondence Address: Gajdács, M.; Department of Pharmacodynamics and Biopharmacy, Eötvös utca 6., Hungary; email: gajdacs.mario@pharm.u-szeged.hu Correspondence Address: Gajdács, M.; Institute of Medical Microbiology, Nagyvárad tér 4., Hungary; email: gajdacs.mario@pharm.u-szeged.hu LA - English DB - MTMT ER -