TY - JOUR AU - Kúthy-Sutus, Enikő AU - Kharrat, Bayan AU - Gábor, Erika AU - Csordás, Gábor AU - Sinka, Rita AU - Honti, Viktor TI - A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 1 PG - 15 SN - 2073-4409 DO - 10.3390/cells12010024 UR - https://m2.mtmt.hu/api/publication/33555087 ID - 33555087 N1 - Export Date: 24 January 2023 AB - The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes following immune challenges and in case of genetic alterations. Since cell culturing has hugely contributed to the understanding of the physiological properties of vertebrate blood cells, this method provides a versatile tool for studying Drosophila hemocyte differentiation and functions ex vivo. LA - English DB - MTMT ER - TY - CONF AU - Kúthy-Sutus, Enikő AU - Gábor, Erika AU - Kharrat, Bayan AU - Jankovics, Ferenc AU - Sinka, Rita AU - Honti, Viktor TI - Studying Drosophila blood cell plasticity using primary blood cell cultures T2 - Straub-Napok 2022 PY - 2022 SP - 1 UR - https://m2.mtmt.hu/api/publication/34689202 ID - 34689202 LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, Bayan AU - Csordás, Gábor AU - Honti, Viktor TI - Peeling Back the Layers of Lymph Gland Structure and Regulation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 14 PG - 16 SN - 1661-6596 DO - 10.3390/ijms23147767 UR - https://m2.mtmt.hu/api/publication/33050458 ID - 33050458 N1 - Cited By :2 Export Date: 24 January 2023 AB - During the past 60 years, the fruit fly, Drosophila melanogaster, has proven to be an excellent model to study the regulation of hematopoiesis. This is not only due to the evolutionarily conserved signalling pathways and transcription factors contributing to blood cell fate, but also to convergent evolution that led to functional similarities in distinct species. An example of convergence is the compartmentalization of blood cells, which ensures the quiescence of hematopoietic stem cells and allows for the rapid reaction of the immune system upon challenges. The lymph gland, a widely studied hematopoietic organ of the Drosophila larva, represents a microenvironment with similar features and functions to classical hematopoietic stem cell niches of vertebrates. Lymph gland studies were effectively supported by the unparalleled toolkit developed in Drosophila, which enabled the high-resolution investigation of the cellular composition and regulatory interaction networks of the lymph gland. In this review, we summarize how our understanding of lymph gland structure and hematopoietic cell-to-cell communication evolved during the past decades and compare their analogous features to those of the vertebrate hematopoietic stem cell niche. LA - English DB - MTMT ER - TY - JOUR AU - Szkalisity, Ábel AU - Piccinini, Filippo AU - Beleon, Attila AU - Balassa, Tamás AU - Varga, Gergely István AU - Migh, Ede AU - Molnár, Csaba AU - Paavolainen, Lassi AU - Timonen, Sanna AU - Banerjee, Indranil AU - Ikonen, Elina AU - Yamauchi, Yohei AU - Andó, István AU - Peltonen, Jaakko AU - Pietiäinen, Vilja AU - Honti, Viktor AU - Horváth, Péter TI - Regression plane concept for analysing continuous cellular processes with machine learning JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 12 PY - 2021 IS - 1 PG - 9 SN - 2041-1723 DO - 10.1038/s41467-021-22866-x UR - https://m2.mtmt.hu/api/publication/32023128 ID - 32023128 N1 - Cited By :2 Export Date: 14 June 2022 LA - English DB - MTMT ER - TY - JOUR AU - Balog, József Ágoston AU - Honti, Viktor AU - Kurucz, Judit Éva AU - Kari, Beáta AU - Puskás, László AU - Andó, István AU - Szebeni, Gábor TI - Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry JF - GENOMICS PROTEOMICS & BIOINFORMATICS J2 - GENOM PROTEOM BIOINF VL - 19 PY - 2021 IS - 2 SP - 243 EP - 252 PG - 10 SN - 1672-0229 DO - 10.1016/j.gpb.2020.06.022 UR - https://m2.mtmt.hu/api/publication/31940120 ID - 31940120 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030, GINOP-2.3.2-15-2016-00035, NKFI NN118207, NKFI K120142, NKFI 120140, OTKA K-131484]; New National Excellence Program of the Ministry for Innovation and Technology, Hungary [UNKP-19-4-SZTE-36]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [BO/00139/17/8] Funding text: This work was supported by the grants from the National Research, Development and Innovation Office, Hungary (Grant Nos. GINOP-2.3.2-15-2016-00001, GINOP-2.3.2-152016-00030 to LGP, GINOP-2.3.2-15-2016-00035 to E ' K, NKFI NN118207 and NKFI K120142 to IA, NKFI 120140 to E ' K, and OTKA K-131484 to VH). Ga ' bor J. Szebeni was supported by the New National Excellence Program of the Ministry for Innovation and Technology, Hungary (Grant No. UNKP-19-4-SZTE-36) and by the Ja ' nos Bolyai Research Scholarship of the Hungarian Academy of Sciences (Grant No. BO/00139/17/8). We are grateful to Mrs. Olga Kovalcsik for the technical help. LA - English DB - MTMT ER - TY - JOUR AU - Csordás, Gábor AU - Gábor, Erika AU - Honti, Viktor TI - There and back again: The mechanisms of differentiation and transdifferentiation in Drosophila blood cells JF - DEVELOPMENTAL BIOLOGY J2 - DEV BIOL VL - 469 PY - 2021 SP - 135 EP - 143 PG - 9 SN - 0012-1606 DO - 10.1016/j.ydbio.2020.10.006 UR - https://m2.mtmt.hu/api/publication/31743832 ID - 31743832 N1 - Cited By :5 Export Date: 14 June 2022 CODEN: DEBIA LA - English DB - MTMT ER - TY - JOUR AU - Kharrat, B AU - Gábor, Erika AU - Sinka, Rita AU - Honti, Viktor TI - Function and Interacting Partners of Headcase in Drosophila Melanogaster JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 12 PY - 2020 IS - 3 SP - 25 EP - 25 PG - 1 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/31891672 ID - 31891672 LA - English DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Varga, GI AU - Géczi, Aliz AU - Bayan, K AU - Migh, E AU - Beleon, A AU - Horvath, P AU - Szeplaki, B AU - Enyedi, M AU - Pinter, L AU - Haracska, L AU - Honti, Viktor TI - Vérsejtek transzdifferenciálódásának vizsgálata a mélytanulás módszerével Drosophila melanogasterben JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 12 PY - 2020 IS - 3 SP - 8 EP - 9 PG - 2 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/31891648 ID - 31891648 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Gábor, Erika AU - Cinege, Gyöngyi Ilona AU - Csordás, Gábor AU - Rusvai, Miklós AU - Honti, Viktor AU - Kolics, Balázs AU - Török, Tibor AU - Williams, Michael J AU - Kurucz, Judit Éva AU - Andó, István TI - Identification of reference markers for characterizing honey bee (Apis mellifera) hemocyte classes JF - DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY J2 - DEV COMP IMMUNOL VL - 109 PY - 2020 SP - 103701 PG - 5 SN - 0145-305X DO - 10.1016/j.dci.2020.103701 UR - https://m2.mtmt.hu/api/publication/31302837 ID - 31302837 N1 - The publication is supported by the EFOP-3.6.3-VEKOP-16-2017-00008 project. The project is co-financed by the European Union and the European Social Fund. Immunology Unit, Institute of Genetics, Biological Research Centre, P.O.Box 521, Szeged, H-6701, Hungary University of Veterinary Medicine, István u. 2., Budapest, 1078, Hungary Department of Plant Science and Biotechnology, University of Pannonia, Georgikon Faculty, Deák F. u. 16., Keszthely, 8360, Hungary Department of Genetics, University of Szeged, Közép Fasor 52, Szeged, 6726, Hungary Functional Pharmacology, Department of Neuroscience, Uppsala University, Husargatan 3, Box 593, Uppsala, 751 24, Sweden Export Date: 10 December 2020 CODEN: DCIMD Correspondence Address: Andó, I.; Immunology Unit, Institute of Genetics, Biological Research Centre, P.O.Box 521, Hungary; email: ando@brc.hu AB - Cell mediated immunity of the honey bee (Apis mellifera) involves the activity of several hemocyte populations, currently defined by morphological features and lectin binding characteristics. The objective of the present study was to identify molecular markers capable of characterizing subsets of honey bee hemocytes. We developed and employed monoclonal antibodies with restricted reactions to functionally distinct hemocyte subpopulations. Melanizing cells, known as oenocytoids, were defined by an antibody to prophenoloxidase, aggregating cells were identified by the expression of Hemolectin, and phagocytic cells were identified by a marker expressed on granulocytes. We anticipate that this combination of antibodies not only allows for the detection of functionally distinct hemocyte subtypes, but will help to further the exploration of hematopoietic compartments, as well as reveal details of the honey bee cellular immune defense against parasites and microbes. LA - English DB - MTMT ER - TY - JOUR AU - Varga, GI AU - Csordas, G AU - Jankovics, F AU - Cinege, Gyöngyi Ilona AU - Sinka, Rita AU - Kurucz, E AU - Andó, István AU - Honti, Viktor TI - Headcase Regulates Hemocyte Differentiation in Drosophila Melanogaster JF - IMMUNOLÓGIAI SZEMLE J2 - IMMUNOLÓGIAI SZEMLE VL - 11 PY - 2019 IS - 3 SP - 20 EP - 21 PG - 2 SN - 2061-0203 UR - https://m2.mtmt.hu/api/publication/30864553 ID - 30864553 LA - English DB - MTMT ER -