TY - JOUR AU - Virág, Dávid AU - Schlosser, Gitta AU - Borbély, Adina AU - Gellén, Gabriella AU - Papp, Dávid AU - Kaleta, Zoltán AU - Dalmadiné Kiss, Borbála AU - Antal, István AU - Ludányi, Krisztina TI - A Mass Spectrometry Strategy for Protein Quantification Based on the Differential Alkylation of Cysteines Using Iodoacetamide and Acrylamide JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 SP - 4656 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25094656 UR - https://m2.mtmt.hu/api/publication/34821651 ID - 34821651 AB - Mass spectrometry has become the most prominent yet evolving technology in quantitative proteomics. Today, a number of label-free and label-based approaches are available for the relative and absolute quantification of proteins and peptides. However, the label-based methods rely solely on the employment of stable isotopes, which are expensive and often limited in availability. Here we propose a label-based quantification strategy, where the mass difference is identified by the differential alkylation of cysteines using iodoacetamide and acrylamide. The alkylation reactions were performed under identical experimental conditions; therefore, the method can be easily integrated into standard proteomic workflows. Using high-resolution mass spectrometry, the feasibility of this approach was assessed with a set of tryptic peptides of human serum albumin. Several critical questions, such as the efficiency of labeling and the effect of the differential alkylation on the peptide retention and fragmentation, were addressed. The concentration of the quality control samples calculated against the calibration curves were within the ±20% acceptance range. It was also demonstrated that heavy labeled peptides exhibit a similar extraction recovery and matrix effect to light ones. Consequently, the approach presented here may be a viable and cost-effective alternative of stable isotope labeling strategies for the quantification of cysteine-containing proteins. LA - English DB - MTMT ER - TY - JOUR AU - Szederkényi, G. AU - Kocsis, Dorottya AU - Vághy, M.A. AU - Czárán, Domonkos Tamás AU - Sasvári, Péter AU - Lengyel, Miléna AU - Naszlady, M.B. AU - Kreis, F. AU - Antal, István AU - Csépányi-Kömi, Roland AU - Erdő, F. TI - Mathematical modeling of transdermal delivery of topical drug formulations in a dynamic microfluidic diffusion chamber in health and disease JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 4 PG - 17 SN - 1932-6203 DO - 10.1371/journal.pone.0299501 UR - https://m2.mtmt.hu/api/publication/34813473 ID - 34813473 AB - Mathematical models of epidermal and dermal transport are essential for optimization and development of products for percutaneous delivery both for local and systemic indication and for evaluation of dermal exposure to chemicals for assessing their toxicity. These models often help directly by providing information on the rate of drug penetration through the skin and thus on the dermal or systemic concentration of drugs which is the base of their pharmacological effect. The simulations are also helpful in analyzing experimental data, reducing the number of experiments and translating the in vitro investigations to an in-vivo setting. In this study skin penetration of topically administered caffeine cream was investigated in a skin-on-a-chip microfluidic diffusion chamber at room temperature and at 32̊C. Also the transdermal penetration of caffeine in healthy and diseased conditions was compared in mouse skins from intact, psoriatic and allergic animals. In the last experimental setup dexamethasone, indomethacin, piroxicam and diclofenac were examined as a cream formulation for absorption across the dermal barrier. All the measured data were used for making mathematical simulation in a three-compartmental model. The calculated and measured results showed a good match, which findings indicate that our mathematical model might be applied for prediction of drug delivery through the skin under different circumstances and for various drugs in the novel, miniaturized diffusion chamber. © 2024 Szederkényi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, LA - English DB - MTMT ER - TY - JOUR AU - Kállai-Szabó, Nikolett AU - Farkas, Dóra AU - Lengyel, Miléna AU - Basa, Bálint AU - Fleck, C. AU - Antal, István TI - Microparticles and multi-unit systems for advanced drug delivery JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 194 PY - 2024 PG - 25 SN - 0928-0987 DO - 10.1016/j.ejps.2024.106704 UR - https://m2.mtmt.hu/api/publication/34631071 ID - 34631071 N1 - Export Date: 28 February 2024 CODEN: EPSCE Correspondence Address: Antal, I.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: antal.istvan@pharma.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - MAGRAMANE, Sabrina AU - Vlahovic, Kristina AU - Gordon, Péter AU - Kállai-Szabó, Nikolett AU - Zelkó, Romána AU - Antal, István AU - Farkas, Dóra TI - Inhalation Dosage Forms: A Focus on Dry Powder Inhalers and Their Advancements JF - PHARMACEUTICALS J2 - PHARMACEUTICALS-BASE VL - 16 PY - 2023 IS - 12 PG - 48 SN - 1424-8247 DO - 10.3390/ph16121658 UR - https://m2.mtmt.hu/api/publication/34405975 ID - 34405975 N1 - Department of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, Budapest, H-1092, Hungary Department of Electronics Technology, Budapest University of Technology and Economics, Egry J. Str. 18, Budapest, H-1111, Hungary Department of Pharmacy Administration, Semmelweis University, Hőgyes Str. 7–9, Budapest, H-1092, Hungary Export Date: 14 March 2024 Correspondence Address: Kállai-Szabó, N.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: kallai.nikolett@semmelweis.hu Correspondence Address: Farkas, D.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: farkas.dora@semmelweis.hu AB - In this review, an extensive analysis of dry powder inhalers (DPIs) is offered, focusing on their characteristics, formulation, stability, and manufacturing. The advantages of pulmonary delivery were investigated, as well as the significance of the particle size in drug deposition. The preparation of DPI formulations was also comprehensively explored, including physico-chemical characterization of powders, powder processing techniques, and formulation considerations. In addition to manufacturing procedures, testing methods were also discussed, providing insights into the development and evaluation of DPI formulations. This review also explores the design basics and critical attributes specific to DPIs, highlighting the significance of their optimization to achieve an effective inhalation therapy. Additionally, the morphology and stability of 3 DPI capsules (Spiriva, Braltus, and Onbrez) were investigated, offering valuable insights into the properties of these formulations. Altogether, these findings contribute to a deeper understanding of DPIs and their development, performance, and optimization of inhalation dosage forms. LA - English DB - MTMT ER - TY - GEN AU - Antal, István TI - Drug delivery system for the improvement of patient centricity, compliance, and adherence PY - 2023 UR - https://m2.mtmt.hu/api/publication/34128875 ID - 34128875 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Gergő D. AU - Kállai-Szabó, Nikolett AU - Lengyel, Miléna AU - Süvegh, Károly AU - Ender, Ferenc AU - Katona, Gábor AU - Nochta-Kazsoki, Adrienn Katalin AU - Zelkó, Romána AU - Antal, István AU - Balogh, György Tibor AU - Balogh Weiser, Diána TI - Nanoformulation of lipase from Porcine pancreas by electrospinning as a novel alternative for enzyme-based per os therapies JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 389 PY - 2023 PG - 13 SN - 0167-7322 DO - 10.1016/j.molliq.2023.122819 UR - https://m2.mtmt.hu/api/publication/34096036 ID - 34096036 N1 - Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Department of Pharmaceutics, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Eötvös Loránd University Laboratory of Nuclear Chemistry, P.O. Box 32, Budapest, H-1518, Hungary Department of Electron Devices, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Spinsplit Llc., Vend u. 17, Budapest, H-1025, Hungary Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary University Pharmacy, Department of Pharmacy Administration, Faculty of Pharmaceutical Sciences, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Semmelweis University, Hőgyes E. Street 7–9, Budapest, H-1092, Hungary Department of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Budapest, H-1111, Hungary Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 20 February 2024 CODEN: JMLID Correspondence Address: Antal, I.; Department of Pharmaceutical Chemistry, Hőgyes E. Street 7–9, Hungary; email: antal.istvan@semmelweis.hu LA - English DB - MTMT ER - TY - JOUR AU - Budai, Lívia AU - Budai, Marianna AU - Pápay, Zsófia Edit AU - Szalkai, Petra AU - Niczinger, Noémi AU - Kijima, S. AU - Sugibayashi, K. AU - Antal, István AU - Kállai-Szabó, Nikolett TI - Viscoelasticity of Liposomal Dispersions JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 13 PY - 2023 IS - 16 PG - 14 SN - 2079-4991 DO - 10.3390/nano13162340 UR - https://m2.mtmt.hu/api/publication/34096011 ID - 34096011 N1 - Department of Pharmaceutics, Semmelweis University, Hőgyes Str. 7, Budapest, 1092, Hungary Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Saitama, Sakado, 350-0295, Japan Export Date: 21 November 2023 Correspondence Address: Antal, I.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: antal.istvan@semmelweis.hu Correspondence Address: Kállai-Szabó, N.; Department of Pharmaceutics, Hőgyes Str. 7, Hungary; email: kallai.nikolett@semmelweis.hu LA - English DB - MTMT ER - TY - JOUR AU - Budai, Lívia AU - Budai, Marianna AU - Bozó, Tamás AU - Agócs, Gergely AU - Kellermayer, Miklós AU - Antal, István TI - Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 13 PG - 13 SN - 1420-3049 DO - 10.3390/molecules28135125 UR - https://m2.mtmt.hu/api/publication/34041182 ID - 34041182 AB - Knowledge of the physical and chemical properties of phospholipids, such as phase transition temperatures (Tc), is of great importance in order to reveal the functionalities of biological and artificial membranes. Our research group developed an oscillatory rheological method for the simple and rapid determination of phase transition temperatures (Tc). The phospholipids constructing the membranes undergo conformational changes at their Tc, which cause alterations of viscoelastic properties of the molecules. The oscillatory technique recommended by us proved to be appropriate to reveal the altered molecular properties of phospholipids as tracking the slightest changes in the viscoelasticity. Our study demonstrates the abrupt changes in rheological properties at Tc for the following phospholipids: 1,2-Dimyristoyl-sn-glycero-3-Phosphocholine (DMPC), 1,2-Dipalmitoyl-sn-glycero-3-Phosphatidylcholine (DPPC), and 1,2-Distearoyl-sn-glycero-3-Phosphocholine (DSPC), proving that the applied methodology is adequate for determining the Tc of phospholipids. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Dóra AU - Ostorházi, Eszter AU - Stercz, Balázs AU - Makra, Nóra AU - Pénzes, Kinga AU - Kristóf, Katalin AU - Antal, István AU - Réthelyi, János AU - Zsigmond, Réka Ildikó AU - Birtalan, Ede AU - Merkely, Béla Péter AU - Tamás, László TI - Specific nasopharyngeal Corynebacterium strains serve as gatekeepers against SARS-CoV-2 infection JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE VL - 45 PY - 2023 IS - 5 SP - 2927 EP - 2938 PG - 12 SN - 2509-2715 DO - 10.1007/s11357-023-00850-1 UR - https://m2.mtmt.hu/api/publication/34026885 ID - 34026885 AB - The SARS-CoV-2 virus is still causing a worldwide problem. The virus settles primarily on the nasal mucosa, and the infection and its course depend on individual susceptibility. Our aim was to investigate the nasopharynx composition’s role in the individual susceptibility. During the first phase of SARS-CoV-2 pandemic, nasopharyngeal microbiome samples of close contact unvaccinated patients were investigated by 16S rRNA analysis and by culturing. The whole genome of cultured Corynebacteria was sequenced. The relative expression of ACE2, TMPRSS2, and cathepsin L on Caco-2 cells and the strength of S1-ACE2 binding were determined in the presence of Corynebacteria . From 55 close contacts exposed to identical SARS-CoV-2 exposure, 26 patients became infected and 29 remained uninfected. The nasopharyngeal microbiome analysis showed significantly higher abundance of Corynebacteria in uninfected group. Corynebacterium accolens could be cultivated only from uninfected individuals and Corynebacterium propinquum from both infected and uninfected. Corynebacteria from uninfected patient significantly reduced the ACE2 and cathepsin L expression. C. accolens significantly reduced the TMPRSS2 expression compared to other Corynebacteria . Furthermore, Corynebacterium spp. weakened the binding of the S1-ACE2. Most C. accolens isolates harbored the TAG lipase LipS1 gene. Based on these results, the presence of Corynebacterium spp. in the nasopharyngeal microbiota, especially C. accolens strains, could reduce the individual susceptibility to SARS-CoV-2 infection by several mechanisms: by downregulation the ACE2, the TMPRSS2 receptors, and cathepsin L in the host; through the inhibition of S1-ACE2 binding; and lipase production. These results suggest the use of C. accolens strains as probiotics in the nasopharynx in the future. LA - English DB - MTMT ER - TY - JOUR AU - Budai, Lívia AU - Budai, Marianna AU - Pápay, Zsófia Edit AU - Vilimi, Zsófia AU - Antal, István TI - Rheological Considerations of Pharmaceutical Formulations: Focus on Viscoelasticity JF - GELS (BASEL) J2 - GELS-BASEL VL - 9 PY - 2023 IS - 6 PG - 20 SN - 2310-2861 DO - 10.3390/gels9060469 UR - https://m2.mtmt.hu/api/publication/34009564 ID - 34009564 AB - Controlling rheological properties offers the opportunity to gain insight into the physical characteristics, structure, stability and drug release rate of formulations. To better understand the physical properties of hydrogels, not only rotational but also oscillatory experiments should be performed. Viscoelastic properties, including elastic and viscous properties, are measured using oscillatory rheology. The gel strength and elasticity of hydrogels are of great importance for pharmaceutical development as the application of viscoelastic preparations has considerably expanded in recent decades. Viscosupplementation, ophthalmic surgery and tissue engineering are just a few examples from the wide range of possible applications of viscoelastic hydrogels. Hyaluronic acid, alginate, gellan gum, pectin and chitosan are remarkable representatives of gelling agents that attract great attention applied in biomedical fields. This review provides a brief summary of rheological properties, highlighting the viscoelasticity of hydrogels with great potential in biomedicine. LA - English DB - MTMT ER -