@article{MTMT:34425078,
	title = {Nanoinjection of extracellular vesicles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/34425078},
	author = {Kovács, Kinga Dóra and Visnovitz, Tamás and Gerecsei, Tamás and Péter, Beatrix and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and Visnovitzné Dr Vukman, Krisztina and Balogh, Anna and Rajmon, Imola and Lőrincz, Péter and Székács, Inna and Buzás, Edit Irén and Horváth, Róbert},
	doi = {10.1002/jev2.12388},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {12},
	unique-id = {34425078},
	abstract = {In the past decade, extracellular vesicles (EVs) have attracted substantial interest in biomedicine. With progress in the field, we have an increasing understanding of cellular responses to EVs. In this Technical Report, we describe the direct nanoinjection of EVs into the cytoplasm of single cells of different cell lines. By using robotic fluidic force microscopy (robotic FluidFM), nanoinjection of GFP positive EVs and EV‐like particles into single live HeLa, H9c2, MDA‐MB‐231 and LCLC‐103H cells proved to be feasible. This injection platform offered the advantage of high cell selectivity and efficiency. The nanoinjected EVs were initially localized in concentrated spot‐like regions within the cytoplasm. Later, they were transported towards the periphery of the cells. Based on our proof‐of‐principle data, robotic FluidFM is suitable for targeting single living cells by EVs and may lead to information about intracellular EV cargo delivery at a single‐cell level.},
	year = {2023},
	eissn = {2001-3078},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Lőrincz, Péter/0000-0001-7374-667X; Buzás, Edit Irén/0000-0002-3744-206X; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:34239207,
	title = {A comparative analysis of fruit fly and human glutamate dehydrogenases in Drosophila melanogaster sperm development},
	url = {https://m2.mtmt.hu/api/publication/34239207},
	author = {Vedelek, Viktor and Vedelek, Balázs and Lőrincz, Péter and Juhász, Gábor and Sinka, Rita},
	doi = {10.3389/fcell.2023.1281487},
	journal-iso = {FRONT CELL DEV BIOL},
	journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY},
	volume = {11},
	unique-id = {34239207},
	issn = {2296-634X},
	abstract = {Glutamate dehydrogenases are enzymes that take part in both amino acid and energy metabolism. Their role is clear in many biological processes, from neuronal function to cancer development. The putative testis-specific Drosophila glutamate dehydrogenase, Bb8, is required for male fertility and the development of mitochondrial derivatives in spermatids. Testis-specific genes are less conserved and could gain new functions, thus raising a question whether Bb8 has retained its original enzymatic activity. We show that while Bb8 displays glutamate dehydrogenase activity, there are significant functional differences between the housekeeping Gdh and the testis-specific Bb8. Both human GLUD1 and GLUD2 can rescue the bb8 ms mutant phenotype, with superior performance by GLUD2. We also tested the role of three conserved amino acids observed in both Bb8 and GLUD2 in Gdh mutants, which showed their importance in the glutamate dehydrogenase function. The findings of our study indicate that Drosophila Bb8 and human GLUD2 could be novel examples of convergent molecular evolution. Furthermore, we investigated the importance of glutamate levels in mitochondrial homeostasis during spermatogenesis by ectopic expression of the mitochondrial glutamate transporter Aralar1, which caused mitochondrial abnormalities in fly spermatids. The data presented in our study offer evidence supporting the significant involvement of glutamate metabolism in sperm development.},
	year = {2023},
	eissn = {2296-634X},
	orcid-numbers = {Vedelek, Balázs/0000-0001-6981-0026; Lőrincz, Péter/0000-0001-7374-667X; Juhász, Gábor/0000-0001-8548-8874; Sinka, Rita/0000-0003-4040-4184}
}

@article{MTMT:34021340,
	title = {Interaction of the sorting nexin 25 homologue Snazarus with Rab11 balances endocytic and secretory transport and maintains the ultrafiltration diaphragm in nephrocytes},
	url = {https://m2.mtmt.hu/api/publication/34021340},
	author = {Maruzs, Tamás and Feil-Börcsök, Dalma and Lakatos, Enikő and Juhász, Gábor and Blastyák, András and Hargitai, Dávid and Jean, Steve and Lőrincz, Péter and Juhász, Gábor},
	doi = {10.1091/mbc.E22-09-0421},
	journal-iso = {MOL BIOL CELL},
	journal = {MOLECULAR BIOLOGY OF THE CELL},
	volume = {34},
	unique-id = {34021340},
	issn = {1059-1524},
	abstract = {Proper balance of exocytosis and endocytosis is important for the maintenance of plasma membrane lipid and protein homeostasis. This is especially critical in human podocytes and the podocyte-like Drosophila nephrocytes that both use a delicate diaphragm system with evolutionarily conserved components for ultrafiltration. Here we show that the sorting nexin 25 homolog Snazarus (Snz) binds to Rab11 and localizes to Rab11-positive recycling endosomes in Drosophila nephrocytes, unlike in fat cells where it is present in plasma membrane/lipid droplet/ER contact sites. Loss of Snz leads to redistribution of Rab11 vesicles from the cell periphery and increases endocytic activity in nephrocytes. These changes are accompanied by defects in diaphragm protein distribution that resemble those seen in Rab11 gain-of-function cells. Of note, co-overexpression of Snz rescues diaphragm defects in Rab11 overexpressing cells, whereas snz knockdown in Rab11 overexpressing nephrocytes or simultaneous knockdown of snz and tbc1d8b encoding a Rab11 GAP lead to massive expansion of the lacunar system that contains mislocalized diaphragm components: Sns and Pyd/ZO-1. We find that loss of Snz enhances while its overexpression impairs secretion, which, together with genetic epistasis analyses, suggest that Snz counteracts Rab11 to maintain the diaphragm via setting the proper balance of exocytosis and endocytosis.},
	year = {2023},
	eissn = {1939-4586},
	orcid-numbers = {Maruzs, Tamás/0000-0001-8142-3221; Lőrincz, Péter/0000-0001-7374-667X; Juhász, Gábor/0000-0001-8548-8874}
}

@article{MTMT:33750753,
	title = {Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines},
	url = {https://m2.mtmt.hu/api/publication/33750753},
	author = {Koncz, Anna and Turiák, Lilla and Németh, Krisztina and Lenzinger, Dorina and Bárkai, Tünde and Lőrincz, Péter and Zelenyánszki, Helga and Visnovitzné Dr Vukman, Krisztina and Buzás, Edit Irén and Visnovitz, Tamás},
	doi = {10.3390/membranes13040431},
	journal-iso = {MEMBRANES-BASEL},
	journal = {MEMBRANES (BASEL)},
	volume = {13},
	unique-id = {33750753},
	abstract = {Cardiomyopathies are leading causes of human mortality. Recent data indicate that the cardiomyocyte-derived extracellular vesicles (EVs) released upon cardiac injury are present in circulation. This paper aimed to analyze EVs released under normal and hypoxic conditions by H9c2 (rat), AC16 (human) and HL1 (mouse) cardiac cell lines. Small (sEVs), medium (mEVs) and large EVs (lEVs) were separated from a conditioned medium by a combination of gravity filtration, differential centrifugation and tangential flow filtration. The EVs were characterized by microBCA, SPV lipid assay, nanoparticle tracking analysis, transmission and immunogold electron microscopy, flow cytometry and Western blotting. Proteomic profiles of the EVs were determined. Surprisingly, an endoplasmic reticulum chaperone, endoplasmin (ENPL, grp94 or gp96), was identified in the EV samples, and its association with EVs was validated. The secretion and uptake of ENPL was followed by confocal microscopy using GFP-ENPL fusion protein expressing HL1 cells. We identified ENPL as an internal cargo of cardiomyocyte-derived mEVs and sEVs. Based on our proteomic analysis, its presence in EVs was linked to hypoxia in HL1 and H9c2 cells, and we hypothesize that EV-associated ENPL may have a cardioprotective role by reducing cardiomyocyte ER stress.},
	year = {2023},
	eissn = {2077-0375},
	orcid-numbers = {Koncz, Anna/0000-0003-2511-2394; Németh, Krisztina/0000-0002-3825-2137; Lőrincz, Péter/0000-0001-7374-667X; Zelenyánszki, Helga/0000-0001-6768-3748; Buzás, Edit Irén/0000-0002-3744-206X; Visnovitz, Tamás/0000-0002-7962-5083}
}

@article{MTMT:33577132,
	title = {MiR-128-3p as blood based liquid biopsy biomarker in childhood acute lymphoblastic leukemia},
	url = {https://m2.mtmt.hu/api/publication/33577132},
	author = {Rzepiel, Andrea and Horváth, Anna and Kutszegi, Nóra and Gézsi, András and C. Sági, Judit and Almási, Laura and Egyed, Bálint and Lőrincz, Péter and Visnovitz, Tamás and Kovács, Gábor and Szalai, Csaba and Semsei F, Ágnes and Erdélyi, Dániel},
	doi = {10.1016/j.mcp.2023.101893},
	journal-iso = {MOL CELL PROBE},
	journal = {MOLECULAR AND CELLULAR PROBES},
	volume = {67},
	unique-id = {33577132},
	issn = {0890-8508},
	year = {2023},
	eissn = {1096-1194},
	orcid-numbers = {Rzepiel, Andrea/0000-0002-7132-7954; Kutszegi, Nóra/0000-0001-5957-3215; Gézsi, András/0000-0003-1022-6356; C. Sági, Judit/0000-0003-2186-9357; Almási, Laura/0000-0001-5615-8948; Egyed, Bálint/0000-0001-8783-9025; Lőrincz, Péter/0000-0001-7374-667X; Visnovitz, Tamás/0000-0002-7962-5083; Kovács, Gábor/0000-0001-9924-1645; Szalai, Csaba/0000-0001-6562-0778; Semsei F, Ágnes/0000-0002-8709-2459; Erdélyi, Dániel/0000-0001-5544-9209}
}

@article{MTMT:33542161,
	title = {Rab26 controls secretory granule maturation and breakdown in Drosophila},
	url = {https://m2.mtmt.hu/api/publication/33542161},
	author = {Boda, Attila and Varga, Luca Petra and Nagy, Anikó Zsuzsanna and Szenci, Győző and Csizmadia, Tamás and Lőrincz, Péter and Juhász, Gábor},
	doi = {10.1007/s00018-022-04674-8},
	journal-iso = {CELL MOL LIFE SCI},
	journal = {CELLULAR AND MOLECULAR LIFE SCIENCES},
	volume = {80},
	unique-id = {33542161},
	issn = {1420-682X},
	abstract = {At the onset of Drosophila metamorphosis, plenty of secretory glue granules are released from salivary gland cells and the glue is deposited on the ventral side of the forming (pre)pupa to attach it to a dry surface. Prior to this, a poorly understood maturation process takes place during which secretory granules gradually grow via homotypic fusions, and their contents are reorganized. Here we show that the small GTPase Rab26 localizes to immature (smaller, non-acidic) glue granules and its presence prevents vesicle acidification. Rab26 mutation accelerates the maturation, acidification and release of these secretory vesicles as well as the lysosomal breakdown (crinophagy) of residual, non-released glue granules. Strikingly, loss of Mon1, an activator of the late endosomal and lysosomal fusion factor Rab7, results in Rab26 remaining associated even with the large glue granules and a concomitant defect in glue release, similar to the effects of Rab26 overexpression. Our data thus identify Rab26 as a key regulator of secretory vesicle maturation that promotes early steps (vesicle growth) and inhibits later steps (lysosomal transport, acidification, content reorganization, release, and breakdown), which is counteracted by Mon1.},
	year = {2023},
	eissn = {1420-9071},
	orcid-numbers = {Boda, Attila/0000-0003-1811-8595; Varga, Luca Petra/0000-0002-6211-8245; Nagy, Anikó Zsuzsanna/0000-0002-0979-1617; Szenci, Győző/0000-0003-0359-6869; Csizmadia, Tamás/0000-0002-2098-9165; Lőrincz, Péter/0000-0001-7374-667X; Juhász, Gábor/0000-0001-8548-8874}
}

@misc{MTMT:33589125,
	title = {Nanoinjection of fluorescent nanoparticles to single live cells by robotic fluidic force microscopy},
	url = {https://m2.mtmt.hu/api/publication/33589125},
	author = {Gerecsei, Tamás and Visnovitz, Tamás and Kovács, Kinga Dóra and Péter, Beatrix and Kurunczi, Sándor and Koncz, Anna and Németh, Krisztina and Lenzinger, Dorina and Visnovitzné Dr Vukman, Krisztina and Lőrincz, Péter and Székács, Inna and Buzás, Edit Irén and Horváth, Róbert},
	unique-id = {33589125},
	year = {2022},
	orcid-numbers = {Visnovitz, Tamás/0000-0002-7962-5083; Kurunczi, Sándor/0000-0002-6567-5231; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Péter/0000-0001-7374-667X; Buzás, Edit Irén/0000-0002-3744-206X; Horváth, Róbert/0000-0001-8617-2302}
}

@article{MTMT:33190551,
	title = {Ecdysone receptor isoform specific regulation of secretory granule acidification in the larval Drosophila salivary gland},
	url = {https://m2.mtmt.hu/api/publication/33190551},
	author = {Nagy, Anikó Zsuzsanna and Szenci, Győző and Boda, Attila and Al-Lami, Muna Adnan Idan and Csizmadia, Tamás and Lőrincz, Péter and Juhász, Gábor and Lőw, Péter},
	doi = {10.1016/j.ejcb.2022.151279},
	journal-iso = {EUR J CELL BIOL},
	journal = {EUROPEAN JOURNAL OF CELL BIOLOGY},
	volume = {101},
	unique-id = {33190551},
	issn = {0171-9335},
	year = {2022},
	eissn = {1618-1298},
	orcid-numbers = {Nagy, Anikó Zsuzsanna/0000-0002-0979-1617; Szenci, Győző/0000-0003-0359-6869; Boda, Attila/0000-0003-1811-8595; Csizmadia, Tamás/0000-0002-2098-9165; Lőrincz, Péter/0000-0001-7374-667X; Juhász, Gábor/0000-0001-8548-8874; Lőw, Péter/0000-0003-2450-7087}
}

@article{MTMT:33162692,
	title = {Autophagy controls Wolbachia infection upon bacterial damage and in aging Drosophila},
	url = {https://m2.mtmt.hu/api/publication/33162692},
	author = {Hargitai, Dávid and Kenéz, Lili Anna and Al-Lami, Muna Adnan Idan and Szenci, Győző and Lőrincz, Péter and Juhász, Gábor},
	doi = {10.3389/fcell.2022.976882},
	journal-iso = {FRONT CELL DEV BIOL},
	journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY},
	volume = {10},
	unique-id = {33162692},
	issn = {2296-634X},
	abstract = {Autophagy is a conserved catabolic process in eukaryotic cells that degrades intracellular components in lysosomes, often in an organelle-specific selective manner (mitophagy, ERphagy, etc). Cells also use autophagy as a defense mechanism, eliminating intracellular pathogens via selective degradation known as xenophagy. Wolbachia pipientis is a Gram-negative intracellular bacterium, which is one of the most common parasites on Earth affecting approximately half of terrestrial arthropods. Interestingly, infection grants the host resistance against other pathogens and modulates lifespan, so this bacterium resembles an endosymbiont. Here we demonstrate that Drosophila somatic cells normally degrade a subset of these bacterial cells, and autophagy is required for selective elimination of Wolbachia upon antibiotic damage. In line with these, Wolbachia overpopulates in autophagy-compromised animals during aging while its presence fails to affect host lifespan unlike in case of control flies. The autophagic degradation of Wolbachia thus represents a novel antibacterial mechanism that controls the propagation of this unique bacterium, behaving both as parasite and endosymbiont at the same time.},
	year = {2022},
	eissn = {2296-634X},
	orcid-numbers = {Szenci, Győző/0000-0003-0359-6869; Lőrincz, Péter/0000-0001-7374-667X; Juhász, Gábor/0000-0001-8548-8874}
}

@CONFERENCE{MTMT:33113442,
	title = {Korai endoszomális SNARE komplexek vizsgálata és azonosítása},
	url = {https://m2.mtmt.hu/api/publication/33113442},
	author = {Lőrincz, Péter},
	booktitle = {Intézményi ÚNKP Konferencia 2022},
	unique-id = {33113442},
	year = {2022},
	pages = {298},
	orcid-numbers = {Lőrincz, Péter/0000-0001-7374-667X}
}