TY - JOUR AU - Richert, A. AU - Kalwasińska, A. AU - Felföldi, Tamás AU - Szabó, Attila AU - Fehér, D. AU - Dembińska, K. AU - Brzezinska, M.S. TI - Characterization of bacterial biofilms developed on the biodegradable polylactide and polycaprolactone polymers containing birch tar in an aquatic environment JF - MARINE POLLUTION BULLETIN J2 - MAR POLLUT BULL VL - 199 PY - 2024 SN - 0025-326X DO - 10.1016/j.marpolbul.2023.115922 UR - https://m2.mtmt.hu/api/publication/34599661 ID - 34599661 N1 - Department of Genetics, Faculty of Biology and Veterinary Science, Nicolaus Copernicus University in Toruń, Gagarina 11, Torun, 87-100, Poland Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Gagarina 11, Torun, 87-100, Poland Institute of Aquatic Ecology, Centre for Ecological Research, Karolina 29, Budapest, 1113, Hungary Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Lennart Hjelms väg 9, Uppsala, SE-75007, Sweden Department of Microbiology, ELTE Eötvös Loránd University, Pázmány Péter stny. 1/c, Budapest, H-1117, Hungary Export Date: 16 February 2024 CODEN: MPNBA Correspondence Address: Richert, A.; Department of Genetics, Gagarina 11, Poland; email: a.richert@umk.pl LA - English DB - MTMT ER - TY - JOUR AU - Futó, Péter AU - Lengyel, Edina AU - Futó, Máté AU - Németh, Zoltán AU - Pirger, Zsolt AU - Komaromy, Andras AU - Padisák, Judit AU - Felföldi, Tamás AU - Kutasi, József AU - Bernát, Gábor TI - Ecophysiological characterisation of a Klebsormidium strain isolated from a cave environment JF - JOURNAL OF APPLIED PHYCOLOGY J2 - J APPL PHYCOL VL - & PY - 2024 SP - & SN - 0921-8971 DO - 10.1007/s10811-023-03161-2 UR - https://m2.mtmt.hu/api/publication/34451192 ID - 34451192 N1 - Limnology Research Group, Centre for Natural Science, University of Pannonia, 10 Egyetem Str., Veszprém, 8200, Hungary HUN-REN Balaton Limnological Research Institute, 3 Klebelsberg Kuno Str., Tihany, 8237, Hungary Albitech Biotechnological Ltd., 47-49 Berlini Road, Budapest, 1045, Hungary HUN-REN-PE Limnoecology Research Group, 10 Egyetem Str., Veszprém, 8200, Hungary Institute of Aquatic Ecology, HUN-REN Centre for Ecological Research, 29 Karolina Str., Budapest, 1113, Hungary Export Date: 19 March 2024 CODEN: JAPPE Correspondence Address: Futó, P.; Limnology Research Group, 10 Egyetem Str., Hungary; email: peter.futo@phd.mk.uni-pannon.hu AB - Members of the genus Klebsormidium are ubiquitously distributed over the Earth and are among the major biological soil crust (BSC) forming microalgae. Their representatives can be found in terrestrial, aquatic, polar, desert regions and have been investigated so far from various aspects. However, the available information about Klebsormidium isolates from lamp-flora is very limited. In our work, we examined a Klebsormidium strain isolated from a Hungarian cave. The temperature optimum of its photosynthetic performance was tested by oxygen yield measurements and pulse-amplitude-modulated fluorescence, which were completed by determination of specific growth rates at different temperatures, from 10 to 40 °C. In addition, we also evaluated the brassinosteroid (BR) content of these cultures. Our results indicated that the studied microalga is capable of growing from 10 to 40 °C, with a 20–25 °C temperature optimum; these findings were in accordance with the observed hormone levels. Regarding photosynthetic performance, the oxygen yield and chlorophyll fluorescence measurements showed maxima at 30–40 °C and 35–40 °C, respectively. Moreover, the examined Klebsormidium strain demonstrates traits associated with cave adaptation, i.e., by high light utilisation factor (α) and diminished light adaptation parameter (I k ) values. LA - English DB - MTMT ER - TY - JOUR AU - Lange-Enyedi, Nóra AU - Kériné Borsodi, Andrea AU - Németh, Péter AU - Czuppon, György AU - Kovács, Ivett AU - Leél-Őssy, Szabolcs AU - Dobosy, Péter AU - Felföldi, Tamás AU - Demény, Attila AU - Makk, Judit TI - Habitat-related variability in the morphological and taxonomic diversity of microbial communities in two Hungarian epigenic karst caves JF - FEMS MICROBIOLOGY ECOLOGY J2 - FEMS MICROBIOL ECOL VL - 99 PY - 2023 IS - 12 PG - 17 SN - 0168-6496 DO - 10.1093/femsec/fiad161 UR - https://m2.mtmt.hu/api/publication/34431117 ID - 34431117 N1 - Institute for Geological and Geochemical Research, HUN-REN Research Centre for Astronomy and Earth Sciences, Budaörsi út 45, Budapest, H-1112, Hungary Department of Microbiology, Institute of Biology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, H-1117, Hungary Institute of Aquatic Ecology, HUN-REN Centre for Ecological Research, Karolina út 29, Budapest, H-1113, Hungary Research Institute of Biomolecular and Chemical Engineering, Nanolab, University of Pannonia, Egyetem út 10, Veszprém, H-8200, Hungary Department of Physical and Applied Geology, Faculty of Science, Eötvös Loránd University, Pázmány P. sétány 1/C, Budapest, H-1117, Hungary Export Date: 8 February 2024 CODEN: FMECE Correspondence Address: Makk, J.; Department of Microbiology, Pázmány P. sétány 1/C, Hungary; email: makk.judit@ttk.elte.hu AB - The physical and chemical characteristics of the bedrock, along with the geological and hydrological conditions of karst caves may influence the taxonomic and functional diversity of prokaryotes. Most studies so far have focused on microbial communities of caves including only a few samples and have ignored the chemical heterogeneity of different habitat types such as sampling sites, dripping water, carbonate precipitates, cave walls, cave sediment and surface soils connected to the caves. The aim of the present study was to compare the morphology, the composition and physiology of the microbiota in caves with similar environmental parameters (temperature, host rock, elemental and mineral composition of speleothems) but located in different epigenic karst systems. Csodabogyós Cave and Baradla Cave (Hungary) were selected for the analysis of bacterial and archaeal communities using electron microscopy, amplicon sequencing, X-ray diffraction and mass spectroscopic techniques. The microbial communities belonged to the phyla Pseudomonadota, Acidobacteriota, Nitrospirota and Nitrososphaerota, and they showed site-specific variation in composition and diversity. The results indicate that morphological and physiological adaptations provide survival for microorganisms according to the environment. In epigenic karst caves, prokaryotes are prone to increase their adsorption surface, cooperate in biofilms, and implement chemolithoautotrophic growth with different electron-donors and acceptors available in the microhabitats. LA - English DB - MTMT ER - TY - JOUR AU - Lippai, Anett AU - Farkas, Rózsa AU - Szuróczki, Sára AU - Szabó, Attila AU - Felföldi, Tamás AU - Toumi, Marwene AU - M Tóth, Erika TI - Két budapesti termálfürdő mikrobiológiai vizsgálata (Budapest, Magyarország) – a fürdőzés és a medenceüzemeltetés hatása a vízminőségre JF - HIDROLÓGIAI KÖZLÖNY J2 - HIDROL KOZL VL - 103 PY - 2023 IS - 4 SP - 4 EP - 15 PG - 12 SN - 0018-1323 DO - 10.59258/hk.13169 UR - https://m2.mtmt.hu/api/publication/34430575 ID - 34430575 AB - Magyarországon a fürdővizek vizsgálata a jogszabályoknak megfelelően higiénés megközelítésből történik, szabványos módszerek alkalmazásával. Munkánk során két termálfürdő vizsgálatát végeztük el (a kútvizektől a különböző üzemeltetésű medencékig) tenyésztéses módszerekkel, valamint molekuláris módszereket alkalmazva, mint a taxon-specifikus polimeráz láncreakció (PCR), multiplex PCR és az újgenerációs szekvenálás. A termálfürdőkből főként olyan baktériumokat mutattunk ki, amelyek a vizek természetes közösségalkotói, azonban néhány opportunista patogén taxon pl. Pseudomonas aeruginosa, P. stutzeri, Acinetobacter johnsoni, Acinetobacter baumanni, Moraxella osloensis, Microbacterium paraoxydans, Legionella spp., Stenotrophomonas maltophilia és Staphylococcus aureus is megjelent az általunk alkalmazott módszerekkel. A töltő-ürítő üzemeltetésű medencékre magasabb mikroszkópos sejtszám, összes heterotróf csíraszám, micrococcus szám, és magasabb P. aeruginosa és S. aureus szám volt jellemző, mint a vízforgatásos medencékre. Azok a baktériumok, amelyek egyértelműen humán eredettel hozhatók összefüggésbe (pl. bőr eredettel) a medencékben kisebb, mint 1% relatív abundancia értékkel rendelkeztek, előfordulásuk sporadikus (szórványos) volt. Az első, majd az azokat követő 4 hónapon át tartó mintavételek higiénés eredményei azt mutatták, hogy a vízforgatással rendelkező medencék jobb vízminőséggel rendelkeznek, mint a töltő-ürítő üzemeltetésű medencék. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szöllősi, Dávid AU - Hajdrik, Polett AU - Tordai, Hedvig AU - Horváth, Ildikó AU - Veres, Dániel AU - Gillich, Bernadett AU - Shailaja, Kanni Das AU - Smeller, László AU - Bergmann, Ralf Konrad AU - Bachmann, Michael AU - Mihály, Judith AU - Gaál, Anikó AU - Jezsó, Bálint AU - Barátki, Balázs Lajos AU - Kövesdi, Dorottya AU - Bősze, Szilvia AU - Szabó, Ildikó AU - Felföldi, Tamás AU - Oszwald, Erzsébet AU - Padmanabhan, Parasuraman AU - Gulyás, Balázs Zoltán AU - Hamdani, Nazha AU - Máthé, Domokos AU - Varga, Zoltán AU - Szigeti, Krisztián TI - Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-023-45628-9 UR - https://m2.mtmt.hu/api/publication/34267696 ID - 34267696 N1 - Department of Biophysics and Radiation Biology, Semmelweis University, 37-47 Tűzoltó Street, Budapest, 1094, Hungary Institute for Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf, 400 Bautzner Landstraße, Dresden, 01328, Germany Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, 2 Magyar Tudósok Körútja, Budapest, 1117, Hungary Doctoral School of Biology and Institute of Biology, Eötvös Loránd University, 1/C Pázmány Péter Sétány, Budapest, 1117, Hungary Department of Immunology, ELTE Eötvös Loránd University, 1/C Pázmány Péter Sétány, Budapest, 1117, Hungary MTA-ELTE Complement Research Group, Eötvös Loránd Research Network (ELKH), 1/A Pázmány Péter Sétány, Budapest, 1117, Hungary ELKH-ELTE Research Group of Peptide Chemistry, Eötvös L. Research Network, Eötvös L. University, 1/A Pázmány Péter Sétány, Budapest, 1117, Hungary Department of Microbiology, ELTE Eötvös Loránd University, 1/C Pázmány Péter Sétány, Budapest, 1117, Hungary Centre for Ecological Research, Institute of Aquatic Ecology, 29 Karolina Road, Budapest, 1113, Hungary Department of Anatomy, Histology, and Embryology, Semmelweis University, 58 Tűzoltó Street, Budapest, 1094, Hungary Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, Singapore, 30823, Singapore Cognitive Neuroimaging Centre, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore Department of Cellular and Translational Physiology, Institute of Physiology, Ruhr University Bochum, Bochum, 44801, Germany HCEMM-Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Budapest, Budapest, 1089, Hungary CROmed Translational Research Centers, 37-47 Tűzoltó Street, Budapest, 1094, Hungary In Vivo Imaging Advanced Core Facility, Hungarian Center of Excellence for Molecular Medicine (HCEMM), 37-47 Tűzoltó Street, Budapest, 1094, Hungary Export Date: 30 January 2024 Correspondence Address: Szigeti, K.; Department of Biophysics and Radiation Biology, 37-47 Tűzoltó Street, Hungary; email: krisztian.szigeti@gmail.com Funding details: Horizon 2020 Framework Programme, H2020, 739593 Funding details: European Commission, EC, 859890 Funding details: Magyar Tudományos Akadémia, MTA Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI, 2020.1.16-Jövő-2021–00013, 2020–1.1.2-PIACI-KFI-2020–00021, TKP2021-EGA-31 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA, EFOP-1.8.0-VEKOP-17-2017-00001 Funding details: Innovációs és Technológiai Minisztérium Funding text 1: The authors thank Miklós Geiszt for his contributions to the in vivo experiments, Mihály Kálmán and Ferenc Kilin for their help with the TEM measurements, Wouter Jong and Bart van den Berg van Saparoea for the pHbpD(Δd1) plasmid. Funding text 2: Open access funding provided by Semmelweis University. Zoltán Varga was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences, the ÚNKP-21-5 Bolyai + New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. Ildikó Szabó and Szilvia Bősze thank for the support of grant EFOP-1.8.0-VEKOP-17-2017-00001 and for the ELTE Thematic Excellence Programme the 2018-1.2.1-NKP-2018-00005 project (under the 2018-1.2.1-NKP funding scheme) provided by the Hungarian Ministry for Innovation and Technology, Hungary. Kanni Das Shailaja received support from the European Union under project H2020-SmartAge grant Nr. 859890. This work was supported by The European Union’s Horizon 2020 Research And Innovation Program, grant agreement No 739593: HCEMM, supported by EU Programme: H2020-EU.4.a. This work was also partly funded by grants from the Hungarian National Research, Development and Innovation Office (Thematic Excellence Program, TKP-BIOImaging, financed under the 2020–4.1.1-TKP2020 funding scheme, Investment to the Future 2020.1.16-Jövő-2021–00013, TKP2021-EGA-31 and 2020–1.1.2-PIACI-KFI-2020–00021). AB - The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain ( E. coli BL21(DE3) ΔnlpI, ΔlpxM ) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64 Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64 Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs. LA - English DB - MTMT ER - TY - JOUR AU - Świątczak, Joanna AU - Kalwasińska, Agnieszka AU - Felföldi, Tamás AU - Swiontek Brzezinska, Maria TI - Bacillus paralicheniformis 2R5 and its impact on canola growth and N-cycle genes in the rhizosphere JF - FEMS MICROBIOLOGY ECOLOGY J2 - FEMS MICROBIOL ECOL VL - 99 PY - 2023 IS - 9 SN - 0168-6496 DO - 10.1093/femsec/fiad093 UR - https://m2.mtmt.hu/api/publication/34141660 ID - 34141660 AB - Chemical fertilization has a negative impact on the natural environment. Plant growth-promoting (PGP) rhizobacterial biofertilizers can be a safer alternative to synthetic agrochemicals. In this research, a culture-based method was used to assess the population size of rhizobacteria at the vegetative, flowering, and maturity stages of canola. Rhizobacteria were then isolated from each of the canola growth stages, and their seven PGP traits were determined. The highest abundance of culturable bacteria was found at the vegetative stage of the plants. Furthermore, four out of seven PGP traits were produced by the highest % of isolates at the vegetative stage. In the greenhouse experiment that included six rhizobacterial strains with best PGP traits, the greatest canola growth promotion ability under sterile conditions was observed after the introduction of Bacillus paralicheniformis 2R5. Moreover, under nonsterile conditions, 2R5 significantly increased canola growth. The presence of the trpA, B, C, D, E, F and pstA, and S genes in the 2R5 genome could be associated with canola growth promotion abilities. The chiA and mbtH genes could contribute to 2R5 antifungal activity against fungal pathogens. Moreover, the introduction of 2R5 significantly increased the abundance of the narG, nosZ, nifH, and nirS genes, which can prove that the 2R5 strain may be an important member of the soil bacterial community. LA - English DB - MTMT ER - TY - JOUR AU - Márton, Zsuzsanna AU - Csitári, Bianka AU - Felföldi, Tamás AU - Hidas, András AU - Jordán, Ferenc AU - Szabó, Attila AU - Székely, Anna J TI - Contrasting response of microeukaryotic and bacterial communities to the interplay of seasonality and local stressors in shallow soda lakes JF - FEMS MICROBIOLOGY ECOLOGY J2 - FEMS MICROBIOL ECOL VL - 99 PY - 2023 IS - 9 PG - 16 SN - 0168-6496 DO - 10.1093/femsec/fiad095 UR - https://m2.mtmt.hu/api/publication/34124449 ID - 34124449 N1 - Institute of Aquatic Ecology, Centre for Ecological Research, Budapest, H-1113, Hungary National Multidisciplinary Laboratory for Climate Change, Centre for Ecological Research, Budapest, H-1113, Hungary Doctoral School of Environmental Sciences, Eötvös Loránd University, Budapest, H-1117, Hungary Karolinska Institutet, Stockholm, 171 65, Sweden Uppsala University, Uppsala, 752 36, Sweden Department of Microbiology, Eötvös Loránd University, Budapest, H-1117, Hungary Department of Chemistry Life Sciences and Environmental Sustainability, University of Parma, Parma, 43124, Italy Swedish University of Agricultural Sciences, Uppsala, 750 07, Sweden Export Date: 17 October 2023 CODEN: FMECE Correspondence Address: Szekely, A.J.; Uppsala UniversitySweden; email: anna.szekely@slu.se AB - Seasonal environmental variation is a leading driver of microbial planktonic community assembly and interactions. However, departures from usual seasonal trends are often reported. To understand the role of local stressors in modifying seasonal succession, we sampled fortnightly, throughout three seasons, five nearby shallow soda lakes exposed to identical seasonal and meteorological changes. We characterised their microeukaryotic and bacterial communities by amplicon sequencing of the 16S and 18S rRNA gene, respectively. Biological interactions were inferred by analyses of synchronous and time-shifted interaction networks, and the keystone taxa of the communities were topologically identified. The lakes showed similar succession patterns during the study period with spring being characterised by the relevance of trophic interactions and a certain level of community stability followed by a more dynamic and variable summer-autumn period. Adaptation to general seasonal changes happened through shared core microbiome of the lakes. Stochastic events such as desiccation disrupted common network attributes and introduced shifts from the prevalent seasonal trajectory. Our results demonstrated that, despite being extreme and highly variable habitats, shallow soda lakes exhibit certain similarities in the seasonality of their planktonic communities, yet local stressors such as droughts instigate deviations from prevalent trends to a greater extent for microeukaryotic than for bacterial communities. LA - English DB - MTMT ER - TY - JOUR AU - Fiko, Dezso-Robert AU - Raduly, Botond AU - Máthé, István AU - Felföldi, Tamás AU - Lanyi, Szabolcs AU - Szilveszter, Szabolcs TI - Bioaugmentation Potential Investigation Using a Phenol Affinity Analysis of Three Acinetobacter Strains in a Multi-Carbon-Source Condition JF - WATER J2 - WATER-SUI VL - 15 PY - 2023 IS - 15 PG - 14 SN - 2073-4441 DO - 10.3390/w15152815 UR - https://m2.mtmt.hu/api/publication/34109722 ID - 34109722 N1 - Department of Analytical Chemistry and Environmental Engineering, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gheorghe Polizu nr. 1–7, Bucharest, 011061, Romania Department of Bioengineering, Faculty of Economics, Socio-Human Sciences and Engineering, Sapientia Hungarian University of Transylvania, Libertății nr. 1, Miercurea Ciuc, 530100, Romania Department of Microbiology, Eötvös Loránd University, Pázmány Péter Stny. 1/C, Budapest, H-1117, Hungary Export Date: 29 August 2023 Correspondence Address: Szilveszter, S.; Department of Bioengineering, Libertății nr. 1, Romania; email: szilveszterszabolcs@uni.sapientia.ro AB - Bioaugmentation potential and phenol substrate affinity in a multi-carbon-source condition for three Acinetobacter strains (Acinetobacter towneri CFII-87, Acinetobacter johnsonii CFII-99A and Acinetobacter sp. CFII-98) were demonstrated. First, the phenol biodegradation ability of the strains was analyzed in batch experiments with phenol as the sole carbon source. All strains degraded phenol at 100 and 500 mg & BULL;L-1 initial concentrations; the maximum specific growth rates were 0.59 and 0.30 d(-1) for A. towneri CFII-87, 0.50 and 0.20 d(-1) for A. johnsonii CFII-99A, and 0.64 and 0.29 d(-1) for A. sp. CFII-98, respectively. For the two tested phenol concentrations, no lag phase was observed for the A. towneri CFII-87 strain, A. sp. CFII-98 presented 4 h and 8 h lag phase, while A. johnsonii CFII-99A presented 3 h and 12 h lag phases. Phenol carbon source dependency of the strains was tested in a multi-carbon-source condition (on phenol-rich synthetic wastewater), both for individual strains and for a consortium prepared as an equal mixture of the three strains. The strains A. towneri CFII-87 and A. sp. CFII-98 and the consortia degraded phenol in 16 h while there was no other significant carbon source consumption during the 48 h trial, as shown by the constant non-phenolic residual chemical oxygen demand (COD) and volatile suspended solids (VSS) concentration after the depletion of phenol. The strain A. johnsonii CFII-99A, however, consumed phenol within 24 h and a further decrease in non-phenolic COD and increase in biomass was also observed upon the depletion of phenol. The highest specific phenol removal rate of 282.11 mg phenol & BULL;g VSS & BULL;h(-1) was observed in the case of the strain A. towneri CFII-87, followed by A. sp. CFII-98, the consortium and A. johnsonii CFII-99A with 178.84, 146.76 and 141.01 mg phenol & BULL;g VSS & BULL;h(-1), respectively. Two bacterial strains (A. towneri CFII-87, A. sp. CFII-98) presented a strong affinity to phenol, utilizing it as a primary carbon source, and thus, their use in the bioaugmentation of wastewater bioreactors indicated the viable potential to increase the phenol removal rate of these systems. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Hajnalka AU - Abaffyné, Dózsa-Farkas Klára AU - Felföldi, Tamás TI - New insights into the Enchytraeus albidus complex (Annelida, Enchytraeidae), with the description of three new species from seashores in Italy and Croatia JF - EUROPEAN JOURNAL OF TAXONOMY J2 - EUR J TAXON VL - 870 PY - 2023 SP - 107 EP - 145 PG - 39 SN - 2118-9773 DO - 10.5852/ejt.2023.870.2123 UR - https://m2.mtmt.hu/api/publication/34050571 ID - 34050571 N1 - Department of Microbiology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, H-1117, Hungary Hungarian Natural History Museum, Baross utca 13, Budapest, H-1088, Hungary Department of Systematic Zoology and Ecology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, H-1117, Hungary Institute of Aquatic Ecology, Centre for Ecological Research, Karolina út 29, Budapest, H-1113, Hungary Export Date: 17 October 2023 Correspondence Address: Nagy, H.; Department of Microbiology, Pázmány Péter sétány 1/C, Hungary; email: nhajni6@gmail.com AB - Between 2019 and 2021, samplings were carried out from seashores in Italy and Croatia, where specimens were found morphologically similar to the species of the Enchytraeus albidus complex. The taxon Enchytraeus albidus was recently divided into a number of separate species, and the new Italian and Croatian specimens of Enchytraeus proved to be three additional species, all new to science, based on the results of morphological and molecular studies. In this paper, we present the description of these new species, namely Enchytraeus adrianensis sp. nov., Enchytraeus andrasi sp. nov. and Enchytraeus andrasiformis sp. nov., and provide additional morphological data and new reference sequences for E. albidus s. str., E. krumbachi, E. irregularis and further unidentified specimens that belong to the E. albidus group collected formerly from Hungary, Korea, Svalbard and Kerguelen Islands. LA - English DB - MTMT ER - TY - JOUR AU - Korponai, Kristóf AU - Szuróczki, Sára AU - Márton, Zsuzsanna AU - Szabó, Attila AU - Morais, Paula V. AU - Proença, Diogo Neves AU - M Tóth, Erika AU - Boros, Emil AU - Márialigeti, Károly AU - Felföldi, Tamás TI - Habitat distribution of the genus Belliella in continental waters and the description of Belliella alkalica sp. nov., Belliella calami sp. nov. and Belliella filtrata sp. nov. JF - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY J2 - INT J SYST EVOL MICR VL - 73 PY - 2023 IS - 6 SN - 1466-5026 DO - 10.1099/ijsem.0.005928 UR - https://m2.mtmt.hu/api/publication/34026289 ID - 34026289 N1 - Department of Microbiology, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, 1117, Hungary Agricultural Institute, Centre for Agricultural Research, Brunszvik utca 2, Martonvásár, 2462, Hungary Institute of Aquatic Ecology, Centre for Ecological Research, Karolina út 29, Budapest, 1113, Hungary Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, Lennart Hjelms Vag 9, Uppsala, 750 07, Sweden Department of Life Sciences, Centre for Mechanical Engineering, Materials and Processes, University of Coimbra, Calçada Martim de Freitas, Coimbra, 3000-456, Portugal Export Date: 3 July 2023 CODEN: ISEMF Correspondence Address: Felföldi, T.; Department of Microbiology, Pázmány Péter sétány 1/C, Hungary; email: tamas.felfoldi@ttk.elte.hu AB - The genus Belliella belongs to the family Cyclobacteriaceae (order Cytophagales , phylum Bacteroidota ) and harbours aerobic chemoheterotrophic bacteria. Members of this genus were isolated from various aquatic habitats, and our analysis based on global amplicon sequencing data revealed that their relative abundance can reach up to 5–10 % of the bacterioplankton in soda lakes and pans. Although a remarkable fraction of the most frequent genotypes that we identified from continental aquatic habitats is still uncultured, five new alkaliphilic Belliella strains were characterized in detail in this study, which were isolated from three different soda lakes and pans of the Carpathian Basin (Hungary). Cells of all strains were Gram-stain-negative, obligate aerobic, rod-shaped, non-motile and non-spore-forming. The isolates were oxidase- and catalase-positive, red-coloured, but did not contain flexirubin-type pigments; they formed bright red colonies that were circular, smooth and convex. Their major isoprenoid quinone was MK-7 and the predominant fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 containing C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c . The polar lipid profiles contained phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid, and several unidentified lipids and aminolipids. Based on whole-genome sequences, the DNA G+C content was 37.0, 37.1 and 37.8 mol % for strains R4-6 T , DMA-N-10a T and U6F3 T , respectively. The distinction of three new species was confirmed by in silico genomic comparison. Orthologous average nucleotide identity (<85.4 %) and digital DNA–DNA hybridization values (<38.9 %) supported phenotypic, chemotaxonomic and 16S rRNA gene sequence data and, therefore, the following three novel species are proposed: Belliella alkalica sp. nov. (represented by strains R4-6 T =DSM 111903 T =JCM 34281 T =UCCCB122 T and S4-10), Belliella calami sp. nov. (DMA-N-10a T =DSM 107340 T =JCM 34280 T =UCCCB121 T ) and Belliella filtrata sp. nov. (U6F3 T =DSM 111904 T =JCM 34282 T =UCCCB123 T and U6F1). Emended descriptions of species Belliella aquatica , Belliella baltica , Belliella buryatensis , Belliella kenyensis and Belliella pelovolcani are also presented. LA - English DB - MTMT ER -