@article{MTMT:34599661, title = {Characterization of bacterial biofilms developed on the biodegradable polylactide and polycaprolactone polymers containing birch tar in an aquatic environment}, url = {https://m2.mtmt.hu/api/publication/34599661}, author = {Richert, A. and Kalwasińska, A. and Felföldi, Tamás and Szabó, Attila and Fehér, D. and Dembińska, K. and Brzezinska, M.S.}, doi = {10.1016/j.marpolbul.2023.115922}, journal-iso = {MAR POLLUT BULL}, journal = {MARINE POLLUTION BULLETIN}, volume = {199}, unique-id = {34599661}, issn = {0025-326X}, year = {2024}, eissn = {1879-3363}, orcid-numbers = {Felföldi, Tamás/0000-0003-2009-2478} } @article{MTMT:34451192, title = {Ecophysiological characterisation of a Klebsormidium strain isolated from a cave environment}, url = {https://m2.mtmt.hu/api/publication/34451192}, author = {Futó, Péter and Lengyel, Edina and Futó, Máté and Németh, Zoltán and Pirger, Zsolt and Komaromy, Andras and Padisák, Judit and Felföldi, Tamás and Kutasi, József and Bernát, Gábor}, doi = {10.1007/s10811-023-03161-2}, journal-iso = {J APPL PHYCOL}, journal = {JOURNAL OF APPLIED PHYCOLOGY}, volume = {&}, unique-id = {34451192}, issn = {0921-8971}, abstract = {Members of the genus Klebsormidium are ubiquitously distributed over the Earth and are among the major biological soil crust (BSC) forming microalgae. Their representatives can be found in terrestrial, aquatic, polar, desert regions and have been investigated so far from various aspects. However, the available information about Klebsormidium isolates from lamp-flora is very limited. In our work, we examined a Klebsormidium strain isolated from a Hungarian cave. The temperature optimum of its photosynthetic performance was tested by oxygen yield measurements and pulse-amplitude-modulated fluorescence, which were completed by determination of specific growth rates at different temperatures, from 10 to 40 °C. In addition, we also evaluated the brassinosteroid (BR) content of these cultures. Our results indicated that the studied microalga is capable of growing from 10 to 40 °C, with a 20–25 °C temperature optimum; these findings were in accordance with the observed hormone levels. Regarding photosynthetic performance, the oxygen yield and chlorophyll fluorescence measurements showed maxima at 30–40 °C and 35–40 °C, respectively. Moreover, the examined Klebsormidium strain demonstrates traits associated with cave adaptation, i.e., by high light utilisation factor (α) and diminished light adaptation parameter (I k ) values.}, year = {2024}, eissn = {1573-5176}, pages = {&}, orcid-numbers = {Futó, Péter/0009-0005-8564-4327; Lengyel, Edina/0000-0003-3936-4154; Pirger, Zsolt/0000-0001-9039-6966; Komaromy, Andras/0000-0001-7994-3025; Padisák, Judit/0000-0001-8285-2896; Felföldi, Tamás/0000-0003-2009-2478; Bernát, Gábor/0000-0001-6147-9391} } @article{MTMT:34431117, title = {Habitat-related variability in the morphological and taxonomic diversity of microbial communities in two Hungarian epigenic karst caves}, url = {https://m2.mtmt.hu/api/publication/34431117}, author = {Lange-Enyedi, Nóra and Kériné Borsodi, Andrea and Németh, Péter and Czuppon, György and Kovács, Ivett and Leél-Őssy, Szabolcs and Dobosy, Péter and Felföldi, Tamás and Demény, Attila and Makk, Judit}, doi = {10.1093/femsec/fiad161}, journal-iso = {FEMS MICROBIOL ECOL}, journal = {FEMS MICROBIOLOGY ECOLOGY}, volume = {99}, unique-id = {34431117}, issn = {0168-6496}, abstract = {The physical and chemical characteristics of the bedrock, along with the geological and hydrological conditions of karst caves may influence the taxonomic and functional diversity of prokaryotes. Most studies so far have focused on microbial communities of caves including only a few samples and have ignored the chemical heterogeneity of different habitat types such as sampling sites, dripping water, carbonate precipitates, cave walls, cave sediment and surface soils connected to the caves. The aim of the present study was to compare the morphology, the composition and physiology of the microbiota in caves with similar environmental parameters (temperature, host rock, elemental and mineral composition of speleothems) but located in different epigenic karst systems. Csodabogyós Cave and Baradla Cave (Hungary) were selected for the analysis of bacterial and archaeal communities using electron microscopy, amplicon sequencing, X-ray diffraction and mass spectroscopic techniques. The microbial communities belonged to the phyla Pseudomonadota, Acidobacteriota, Nitrospirota and Nitrososphaerota, and they showed site-specific variation in composition and diversity. The results indicate that morphological and physiological adaptations provide survival for microorganisms according to the environment. In epigenic karst caves, prokaryotes are prone to increase their adsorption surface, cooperate in biofilms, and implement chemolithoautotrophic growth with different electron-donors and acceptors available in the microhabitats.}, year = {2023}, eissn = {1574-6941}, orcid-numbers = {Kériné Borsodi, Andrea/0000-0002-3738-7937; Németh, Péter/0000-0001-5592-5877; Czuppon, György/0000-0002-7231-6042; Kovács, Ivett/0000-0001-7015-7909; Felföldi, Tamás/0000-0003-2009-2478; Demény, Attila/0000-0003-0522-9018; Makk, Judit/0000-0001-5768-7622} } @article{MTMT:34430575, title = {Két budapesti termálfürdő mikrobiológiai vizsgálata (Budapest, Magyarország) – a fürdőzés és a medenceüzemeltetés hatása a vízminőségre}, url = {https://m2.mtmt.hu/api/publication/34430575}, author = {Lippai, Anett and Farkas, Rózsa and Szuróczki, Sára and Szabó, Attila and Felföldi, Tamás and Toumi, Marwene and M Tóth, Erika}, doi = {10.59258/hk.13169}, journal-iso = {HIDROL KOZL}, journal = {HIDROLÓGIAI KÖZLÖNY}, volume = {103}, unique-id = {34430575}, issn = {0018-1323}, abstract = {Magyarországon a fürdővizek vizsgálata a jogszabályoknak megfelelően higiénés megközelítésből történik, szabványos módszerek alkalmazásával. Munkánk során két termálfürdő vizsgálatát végeztük el (a kútvizektől a különböző üzemeltetésű medencékig) tenyésztéses módszerekkel, valamint molekuláris módszereket alkalmazva, mint a taxon-specifikus polimeráz láncreakció (PCR), multiplex PCR és az újgenerációs szekvenálás. A termálfürdőkből főként olyan baktériumokat mutattunk ki, amelyek a vizek természetes közösségalkotói, azonban néhány opportunista patogén taxon pl. Pseudomonas aeruginosa, P. stutzeri, Acinetobacter johnsoni, Acinetobacter baumanni, Moraxella osloensis, Microbacterium paraoxydans, Legionella spp., Stenotrophomonas maltophilia és Staphylococcus aureus is megjelent az általunk alkalmazott módszerekkel. A töltő-ürítő üzemeltetésű medencékre magasabb mikroszkópos sejtszám, összes heterotróf csíraszám, micrococcus szám, és magasabb P. aeruginosa és S. aureus szám volt jellemző, mint a vízforgatásos medencékre. Azok a baktériumok, amelyek egyértelműen humán eredettel hozhatók összefüggésbe (pl. bőr eredettel) a medencékben kisebb, mint 1% relatív abundancia értékkel rendelkeztek, előfordulásuk sporadikus (szórványos) volt. Az első, majd az azokat követő 4 hónapon át tartó mintavételek higiénés eredményei azt mutatták, hogy a vízforgatással rendelkező medencék jobb vízminőséggel rendelkeznek, mint a töltő-ürítő üzemeltetésű medencék.}, year = {2023}, eissn = {2939-8495}, pages = {4-15}, orcid-numbers = {Felföldi, Tamás/0000-0003-2009-2478; M Tóth, Erika/0000-0001-9048-5758} } @article{MTMT:34267696, title = {Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display}, url = {https://m2.mtmt.hu/api/publication/34267696}, author = {Szöllősi, Dávid and Hajdrik, Polett and Tordai, Hedvig and Horváth, Ildikó and Veres, Dániel and Gillich, Bernadett and Shailaja, Kanni Das and Smeller, László and Bergmann, Ralf Konrad and Bachmann, Michael and Mihály, Judith and Gaál, Anikó and Jezsó, Bálint and Barátki, Balázs Lajos and Kövesdi, Dorottya and Bősze, Szilvia and Szabó, Ildikó and Felföldi, Tamás and Oszwald, Erzsébet and Padmanabhan, Parasuraman and Gulyás, Balázs Zoltán and Hamdani, Nazha and Máthé, Domokos and Varga, Zoltán and Szigeti, Krisztián}, doi = {10.1038/s41598-023-45628-9}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {13}, unique-id = {34267696}, issn = {2045-2322}, abstract = {The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain ( E. coli BL21(DE3) ΔnlpI, ΔlpxM ) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64 Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64 Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs.}, year = {2023}, eissn = {2045-2322}, orcid-numbers = {Szöllősi, Dávid/0000-0002-3363-3862; Hajdrik, Polett/0000-0002-5452-4892; Tordai, Hedvig/0000-0002-0875-5569; Veres, Dániel/0000-0002-9687-3556; Smeller, László/0000-0002-3643-3268; Gaál, Anikó/0000-0003-4064-1825; Jezsó, Bálint/0000-0002-1306-4797; Szabó, Ildikó/0000-0002-9844-7841; Felföldi, Tamás/0000-0003-2009-2478; Hamdani, Nazha/0000-0002-3053-0008; Varga, Zoltán/0000-0002-5741-2669} } @article{MTMT:34141660, title = {Bacillus paralicheniformis 2R5 and its impact on canola growth and N-cycle genes in the rhizosphere}, url = {https://m2.mtmt.hu/api/publication/34141660}, author = {Świątczak, Joanna and Kalwasińska, Agnieszka and Felföldi, Tamás and Swiontek Brzezinska, Maria}, doi = {10.1093/femsec/fiad093}, journal-iso = {FEMS MICROBIOL ECOL}, journal = {FEMS MICROBIOLOGY ECOLOGY}, volume = {99}, unique-id = {34141660}, issn = {0168-6496}, abstract = {Chemical fertilization has a negative impact on the natural environment. Plant growth-promoting (PGP) rhizobacterial biofertilizers can be a safer alternative to synthetic agrochemicals. In this research, a culture-based method was used to assess the population size of rhizobacteria at the vegetative, flowering, and maturity stages of canola. Rhizobacteria were then isolated from each of the canola growth stages, and their seven PGP traits were determined. The highest abundance of culturable bacteria was found at the vegetative stage of the plants. Furthermore, four out of seven PGP traits were produced by the highest % of isolates at the vegetative stage. In the greenhouse experiment that included six rhizobacterial strains with best PGP traits, the greatest canola growth promotion ability under sterile conditions was observed after the introduction of Bacillus paralicheniformis 2R5. Moreover, under nonsterile conditions, 2R5 significantly increased canola growth. The presence of the trpA, B, C, D, E, F and pstA, and S genes in the 2R5 genome could be associated with canola growth promotion abilities. The chiA and mbtH genes could contribute to 2R5 antifungal activity against fungal pathogens. Moreover, the introduction of 2R5 significantly increased the abundance of the narG, nosZ, nifH, and nirS genes, which can prove that the 2R5 strain may be an important member of the soil bacterial community.}, year = {2023}, eissn = {1574-6941}, orcid-numbers = {Kalwasińska, Agnieszka/0000-0002-2435-567X; Felföldi, Tamás/0000-0003-2009-2478} } @article{MTMT:34124449, title = {Contrasting response of microeukaryotic and bacterial communities to the interplay of seasonality and local stressors in shallow soda lakes}, url = {https://m2.mtmt.hu/api/publication/34124449}, author = {Márton, Zsuzsanna and Csitári, Bianka and Felföldi, Tamás and Hidas, András and Jordán, Ferenc and Szabó, Attila and Székely, Anna J}, doi = {10.1093/femsec/fiad095}, journal-iso = {FEMS MICROBIOL ECOL}, journal = {FEMS MICROBIOLOGY ECOLOGY}, volume = {99}, unique-id = {34124449}, issn = {0168-6496}, abstract = {Seasonal environmental variation is a leading driver of microbial planktonic community assembly and interactions. However, departures from usual seasonal trends are often reported. To understand the role of local stressors in modifying seasonal succession, we sampled fortnightly, throughout three seasons, five nearby shallow soda lakes exposed to identical seasonal and meteorological changes. We characterised their microeukaryotic and bacterial communities by amplicon sequencing of the 16S and 18S rRNA gene, respectively. Biological interactions were inferred by analyses of synchronous and time-shifted interaction networks, and the keystone taxa of the communities were topologically identified. The lakes showed similar succession patterns during the study period with spring being characterised by the relevance of trophic interactions and a certain level of community stability followed by a more dynamic and variable summer-autumn period. Adaptation to general seasonal changes happened through shared core microbiome of the lakes. Stochastic events such as desiccation disrupted common network attributes and introduced shifts from the prevalent seasonal trajectory. Our results demonstrated that, despite being extreme and highly variable habitats, shallow soda lakes exhibit certain similarities in the seasonality of their planktonic communities, yet local stressors such as droughts instigate deviations from prevalent trends to a greater extent for microeukaryotic than for bacterial communities.}, year = {2023}, eissn = {1574-6941}, orcid-numbers = {Márton, Zsuzsanna/0000-0002-7420-5039; Felföldi, Tamás/0000-0003-2009-2478; Hidas, András/0000-0002-6030-8459} } @article{MTMT:34109722, title = {Bioaugmentation Potential Investigation Using a Phenol Affinity Analysis of Three Acinetobacter Strains in a Multi-Carbon-Source Condition}, url = {https://m2.mtmt.hu/api/publication/34109722}, author = {Fiko, Dezso-Robert and Raduly, Botond and Máthé, István and Felföldi, Tamás and Lanyi, Szabolcs and Szilveszter, Szabolcs}, doi = {10.3390/w15152815}, journal-iso = {WATER-SUI}, journal = {WATER}, volume = {15}, unique-id = {34109722}, abstract = {Bioaugmentation potential and phenol substrate affinity in a multi-carbon-source condition for three Acinetobacter strains (Acinetobacter towneri CFII-87, Acinetobacter johnsonii CFII-99A and Acinetobacter sp. CFII-98) were demonstrated. First, the phenol biodegradation ability of the strains was analyzed in batch experiments with phenol as the sole carbon source. All strains degraded phenol at 100 and 500 mg & BULL;L-1 initial concentrations; the maximum specific growth rates were 0.59 and 0.30 d(-1) for A. towneri CFII-87, 0.50 and 0.20 d(-1) for A. johnsonii CFII-99A, and 0.64 and 0.29 d(-1) for A. sp. CFII-98, respectively. For the two tested phenol concentrations, no lag phase was observed for the A. towneri CFII-87 strain, A. sp. CFII-98 presented 4 h and 8 h lag phase, while A. johnsonii CFII-99A presented 3 h and 12 h lag phases. Phenol carbon source dependency of the strains was tested in a multi-carbon-source condition (on phenol-rich synthetic wastewater), both for individual strains and for a consortium prepared as an equal mixture of the three strains. The strains A. towneri CFII-87 and A. sp. CFII-98 and the consortia degraded phenol in 16 h while there was no other significant carbon source consumption during the 48 h trial, as shown by the constant non-phenolic residual chemical oxygen demand (COD) and volatile suspended solids (VSS) concentration after the depletion of phenol. The strain A. johnsonii CFII-99A, however, consumed phenol within 24 h and a further decrease in non-phenolic COD and increase in biomass was also observed upon the depletion of phenol. The highest specific phenol removal rate of 282.11 mg phenol & BULL;g VSS & BULL;h(-1) was observed in the case of the strain A. towneri CFII-87, followed by A. sp. CFII-98, the consortium and A. johnsonii CFII-99A with 178.84, 146.76 and 141.01 mg phenol & BULL;g VSS & BULL;h(-1), respectively. Two bacterial strains (A. towneri CFII-87, A. sp. CFII-98) presented a strong affinity to phenol, utilizing it as a primary carbon source, and thus, their use in the bioaugmentation of wastewater bioreactors indicated the viable potential to increase the phenol removal rate of these systems.}, keywords = {acinetobacter; Bioaugmentation; Phenol degradation; Synthetic wastewater; phenol affinity; carbon source dependency}, year = {2023}, eissn = {2073-4441}, orcid-numbers = {Felföldi, Tamás/0000-0003-2009-2478} } @article{MTMT:34050571, title = {New insights into the Enchytraeus albidus complex (Annelida, Enchytraeidae), with the description of three new species from seashores in Italy and Croatia}, url = {https://m2.mtmt.hu/api/publication/34050571}, author = {Nagy, Hajnalka and Abaffyné, Dózsa-Farkas Klára and Felföldi, Tamás}, doi = {10.5852/ejt.2023.870.2123}, journal-iso = {EUR J TAXON}, journal = {EUROPEAN JOURNAL OF TAXONOMY}, volume = {870}, unique-id = {34050571}, abstract = {Between 2019 and 2021, samplings were carried out from seashores in Italy and Croatia, where specimens were found morphologically similar to the species of the Enchytraeus albidus complex. The taxon Enchytraeus albidus was recently divided into a number of separate species, and the new Italian and Croatian specimens of Enchytraeus proved to be three additional species, all new to science, based on the results of morphological and molecular studies. In this paper, we present the description of these new species, namely Enchytraeus adrianensis sp. nov., Enchytraeus andrasi sp. nov. and Enchytraeus andrasiformis sp. nov., and provide additional morphological data and new reference sequences for E. albidus s. str., E. krumbachi, E. irregularis and further unidentified specimens that belong to the E. albidus group collected formerly from Hungary, Korea, Svalbard and Kerguelen Islands.}, year = {2023}, eissn = {2118-9773}, pages = {107-145}, orcid-numbers = {Nagy, Hajnalka/0000-0001-6798-8701; Abaffyné, Dózsa-Farkas Klára/0000-0003-3372-2838; Felföldi, Tamás/0000-0003-2009-2478} } @article{MTMT:34026289, title = {Habitat distribution of the genus Belliella in continental waters and the description of Belliella alkalica sp. nov., Belliella calami sp. nov. and Belliella filtrata sp. nov.}, url = {https://m2.mtmt.hu/api/publication/34026289}, author = {Korponai, Kristóf and Szuróczki, Sára and Márton, Zsuzsanna and Szabó, Attila and Morais, Paula V. and Proença, Diogo Neves and M Tóth, Erika and Boros, Emil and Márialigeti, Károly and Felföldi, Tamás}, doi = {10.1099/ijsem.0.005928}, journal-iso = {INT J SYST EVOL MICR}, journal = {INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY}, volume = {73}, unique-id = {34026289}, issn = {1466-5026}, abstract = {The genus Belliella belongs to the family Cyclobacteriaceae (order Cytophagales , phylum Bacteroidota ) and harbours aerobic chemoheterotrophic bacteria. Members of this genus were isolated from various aquatic habitats, and our analysis based on global amplicon sequencing data revealed that their relative abundance can reach up to 5–10 % of the bacterioplankton in soda lakes and pans. Although a remarkable fraction of the most frequent genotypes that we identified from continental aquatic habitats is still uncultured, five new alkaliphilic Belliella strains were characterized in detail in this study, which were isolated from three different soda lakes and pans of the Carpathian Basin (Hungary). Cells of all strains were Gram-stain-negative, obligate aerobic, rod-shaped, non-motile and non-spore-forming. The isolates were oxidase- and catalase-positive, red-coloured, but did not contain flexirubin-type pigments; they formed bright red colonies that were circular, smooth and convex. Their major isoprenoid quinone was MK-7 and the predominant fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and summed feature 3 containing C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c . The polar lipid profiles contained phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid, and several unidentified lipids and aminolipids. Based on whole-genome sequences, the DNA G+C content was 37.0, 37.1 and 37.8 mol % for strains R4-6 T , DMA-N-10a T and U6F3 T , respectively. The distinction of three new species was confirmed by in silico genomic comparison. Orthologous average nucleotide identity (<85.4 %) and digital DNA–DNA hybridization values (<38.9 %) supported phenotypic, chemotaxonomic and 16S rRNA gene sequence data and, therefore, the following three novel species are proposed: Belliella alkalica sp. nov. (represented by strains R4-6 T =DSM 111903 T =JCM 34281 T =UCCCB122 T and S4-10), Belliella calami sp. nov. (DMA-N-10a T =DSM 107340 T =JCM 34280 T =UCCCB121 T ) and Belliella filtrata sp. nov. (U6F3 T =DSM 111904 T =JCM 34282 T =UCCCB123 T and U6F1). Emended descriptions of species Belliella aquatica , Belliella baltica , Belliella buryatensis , Belliella kenyensis and Belliella pelovolcani are also presented.}, year = {2023}, eissn = {1466-5034}, orcid-numbers = {Márton, Zsuzsanna/0000-0002-7420-5039; M Tóth, Erika/0000-0001-9048-5758; Márialigeti, Károly/0000-0003-1536-5160; Felföldi, Tamás/0000-0003-2009-2478} }