TY - CONF AU - Dóra, Fanni AU - Csikós, Vivien AU - Láng, Tamás AU - Darai, Luca AU - Szendi, Vivien AU - Tóth, Attila AU - Cservenák, Melinda AU - Dobolyi, Árpád TI - Genomic insights into the effects of social isolation on the medial prefrontal cortex in male rats T2 - PhD Scientific Days 2024 PY - 2024 UR - https://m2.mtmt.hu/api/publication/35208389 ID - 35208389 AB - According to the theory of homeostatic regulation of social behaviors, short social deprivation increases social interactions. However, the neural mechanisms of this regulation remain unknown. The medial prefrontal cortex (mPFC) plays a pivotal role in the regulation of social behavior in vertebrates. Consequently, we postulated that it may also play a role in social homeostasis. To investigate this, we assessed the impact of social isolation on gene expression in the mPFC using RNA sequencing method (RNA-seq) in male rats housed in pairs or solitarily for 10 days. To characterize the behavioral effects of social isolation, the social, anxiety- and depression-like behaviors of the animals were measured using three-chamber, elevated plus maze and forced swim tests, respectively. The isolated animals exhibited reduced sociability and social novelty preference, but increased social interaction and heightened locomotion. There was no change in their aggression, anxiety, or depression-like activity. Transcriptomics analysis revealed differential expression of 46 genes between the groups, some with known or predicted social functions. Based on the KEGG pathway analysis, differentially expressed genes play a key role in neuroactive ligand-receptor interaction, particularly the dopaminergic and peptidergic systems and addiction. Subsequent validation confirmed the decreased level of three altered genes: Regulator of G Protein Signalling 9 (Rgs9), Serotonin Receptor 2c (Htr2c), and Prodynorphin (Pdyn), which are involved in dopaminergic, serotonergic, and peptidergic function, respectively. Antagonizing Htr2c confirmed its role in social novelty discrimination. The findings imply that 10 days of social isolation can significantly affect gene expression affecting monoaminergic and peptidergic systems of the mPFC, which furthers our understanding of the effects of social isolation at the molecular level. Grant support was provided by NAP3 project of the HAS (NAP2022-I-3/2022), the NKFIH OTKA K134221, OTKA K146077, TKP2021-EGA/TKP2021- NVA/TKP2021-NKTA, EFOP-3.6.3-VEKOP-16-2017-00009 and TKP2021-EGA-25 grants. New National Excellence Program and Doctoral Student Scholarship Program of the Co-operative Doctoral Program of NKFIH, and the Gedeon Richter Plc. Centenary Foundation. LA - English DB - MTMT ER - TY - JOUR AU - Vizvári, Zoltán Ákos AU - Győrfi, Nina Rubina AU - Maczko, Gergo AU - Varga, Reka AU - Jakabfi-Csepregi, Rita AU - Sári, Zoltán AU - Füredi, András AU - Bajtai, Eszter AU - Vajda, Flóra AU - Tadity, Vladimir AU - Odry, Péter AU - Karádi, Zoltán György AU - Tóth, Attila TI - Reproducibility analysis of bioimpedance-based self-developed live cell assays JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-024-67061-2 UR - https://m2.mtmt.hu/api/publication/35141270 ID - 35141270 N1 - Department of Environmental Engineering, Faculty of Engineering and Information Technology, University of Pecs, Boszorkany str. 2, Pecs, 7624, Hungary Multidisciplinary Medical and Engineering Cellular Bioimpedance Research Group, Szentagothai Research Centre, University of Pecs, Ifjusag str. 20, Pecs, 7624, Hungary Symbolic Methods in Material Analysis and Tomography Research Group, Faculty of Engineering and Information Technology, University of Pecs, Boszorkany str. 6, Pecs, 7624, Hungary Institute of Physiology, Medical School, University of Pecs, Szigeti str. 12, Pecs, 7624, Hungary Department of Laboratory Medicine, Medical School, University of Pecs, Szigeti str. 12, Pecs, 7624, Hungary Department of Technical Informatics, Faculty of Engineering and Information Technology, University of Pecs, Boszorkany str. 6, Pecs, 7624, Hungary Institute of Molecular Life Sciences, HUN-REN Research Centre for Natural Sciences, Magyar tudosok korutja 2, Budapest, 1117, Hungary Semmelweis University Doctoral School, Ulloi str. 26, Budapest, 1085, Hungary Institute of Technical Physics and Materials Science, HUN-REN Centre for Energy Research, Konkoly-Thege Miklos ut 29-33, Budapest, 1121, Hungary John von Neumann Faculty of Informatics, Óbuda University, Becsi str. 96/B, Budapest, 1034, Hungary Cited By :1 Export Date: 9 September 2024 Correspondence Address: Vizvari, Z.; Department of Environmental Engineering, Boszorkany str. 2, Hungary; email: vizvari.zoltan@mik.pte.hu Funding details: GINOP PLUSZ-2.1.1-21-2022-00249 Funding details: 2020-1.1.2-PIACI-KFI-2020-00173 Funding text 1: The project has been supported by Grants 009-2023-PTE-RK/27 and 011-2023-PTE-RK/4 of the University of P\\u00E9cs. This research is a part of projects GINOP PLUSZ-2.1.1-21-2022-00249 of the \\u00D3buda University and 2020-1.1.2-PIACI-KFI-2020-00173 of the University of Duna\\u00FAjv\\u00E1ros. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Anita AU - Szabó, Evelin AU - László, Kristóf AU - Kertes, Erika AU - Zagorácz, Olga AU - Mintál, Kitti AU - Tóth, Attila AU - Gálosi, Rita AU - Marosné Berta, Beáta AU - Lénárd, László AU - Hormay, Edina AU - László, Bettina Réka AU - Zelena, Dóra AU - Tóth, Zsuzsanna TI - Brain RFamide Neuropeptides in Stress-Related Psychopathologies JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 13 PG - 40 SN - 2073-4409 DO - 10.3390/cells13131097 UR - https://m2.mtmt.hu/api/publication/35135170 ID - 35135170 N1 - Funding Agency and Grant Number: National Brain Research Program (NAP 3.0) of the Hungarian Academy of Sciences; National Research Development and Innovation Office of Hungary [K146086, K141934, K138763, K134221, K120311]; Thematic Excellence Program 2021 Health Sub-program of the Ministry for Innovation and Technology in Hungary [TKP2021-EGA-16]; Ministry of Innovation and Technology of Hungary [TKP2021-EGA-25] Funding text: This study was supported by the National Brain Research Program (NAP 3.0) of the Hungarian Academy of Sciences; the National Research Development and Innovation Office of Hungary (grant numbers K146086, K141934, K138763, K134221 and K120311), and the Thematic Excellence Program 2021 Health Sub-program of the Ministry for Innovation and Technology in Hungary (within the framework of the TKP2021-EGA-16 project of the Pecs University). Project TKP2021-EGA-25 has been implemented with support provided by the Ministry of Innovation and Technology of Hungary. AB - The RFamide peptide family is a group of proteins that share a common C-terminal arginine-phenylalanine-amide motif. To date, the family comprises five groups in mammals: neuropeptide FF, LPXRFamides/RFamide-related peptides, prolactin releasing peptide, QRFP, and kisspeptins. Different RFamide peptides have their own cognate receptors and are produced by different cell populations, although they all can also bind to neuropeptide FF receptors with different affinities. RFamide peptides function in the brain as neuropeptides regulating key aspects of homeostasis such as energy balance, reproduction, and cardiovascular function. Furthermore, they are involved in the organization of the stress response including modulation of pain. Considering the interaction between stress and various parameters of homeostasis, the role of RFamide peptides may be critical in the development of stress-related neuropathologies. This review will therefore focus on the role of RFamide peptides as possible key hubs in stress and stress-related psychopathologies. The neurotransmitter coexpression profile of RFamide-producing cells is also discussed, highlighting its potential functional significance. The development of novel pharmaceutical agents for the treatment of stress-related disorders is an ongoing need. Thus, the importance of RFamide research is underlined by the emergence of peptidergic and G-protein coupled receptor-based therapeutic targets in the pharmaceutical industry. LA - English DB - MTMT ER - TY - CONF AU - Dóra, Fanni AU - Csikós, Vivien AU - Láng, Tamás AU - Darai, Luca AU - Szendi, Vivien AU - Tóth, Attila AU - Cservenák, Melinda AU - Dobolyi, Árpád TI - A szociális izoláció transzkripciós vizsgálata patkányok mediális prefrontális kérgében T2 - Magyar Anatómus Társaság 2024. évi konferenciája: Programfüzet PY - 2024 UR - https://m2.mtmt.hu/api/publication/35079008 ID - 35079008 LA - Hungarian DB - MTMT ER - TY - CONF AU - Mintál, Kitti AU - Tóth, Attila AU - Hormay, Edina AU - Kocsis, B AU - László, Kristóf AU - Bufa, A AU - Marosvölgyi, T AU - Cserjesi, R AU - Vizvári, Zoltán Ákos AU - Lénárd, László AU - Karádi, Zoltán György TI - Autism spectrum disorder associated behavioral symptoms and their relationship with the gastrointestinal microbiome. T2 - 7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet PY - 2024 SP - 71 UR - https://m2.mtmt.hu/api/publication/34846283 ID - 34846283 LA - English DB - MTMT ER - TY - CONF AU - László, Kristóf AU - Vörös, Dávid AU - Kiss, Orsolya AU - László, Bettina Réka AU - Ollmann, Tamás AU - Péczely, László AU - Mintál, Kitti AU - Tóth, Attila AU - Kovács, Anita AU - Zagorácz, Olga AU - Kertes, Erika AU - Kállai, Veronika AU - Marosné Berta, Beáta AU - Karádi, Zoltán György AU - Lénárd, László TI - The intraamydaloid oxytocin ameliorates some autistic-like symptoms in valproate-induced autism rodent model. T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 20 UR - https://m2.mtmt.hu/api/publication/34840828 ID - 34840828 LA - English DB - MTMT ER - TY - CONF AU - Vörös, Dávid AU - Kiss, Orsolya AU - Ollmann, Tamás AU - Mintál, Kitti AU - Péczely, László AU - Zagorácz, Olga AU - Kertes, Erika AU - Kállai, Veronika AU - László, Bettina Réka AU - Marosné Berta, Beáta AU - Tóth, Attila AU - Lénárd, László AU - László, Kristóf TI - Effect of intraamygdaloid oxytocin on social interaction in valproate-induced autism model. T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 237 UR - https://m2.mtmt.hu/api/publication/34840773 ID - 34840773 LA - English DB - MTMT ER - TY - JOUR AU - Marosvölgyi, Tamás AU - Mintál, Kitti AU - Borbásné Farkas, Kornélia AU - Sipos, Zoltán AU - Makszin, Lilla AU - Szabó, Éva AU - Tóth, Attila AU - Kocsis, Béla AU - Kovács, Krisztina AU - Hormay, Edina AU - Lénárd, László AU - Karádi, Zoltán György AU - Bufa, Anita TI - Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-024-57046-6 UR - https://m2.mtmt.hu/api/publication/34745300 ID - 34745300 N1 - * Megosztott szerzőség AB - Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host’s physiological processes. LA - English DB - MTMT ER - TY - CONF AU - Hormay, Edina AU - Mintál, Kitti AU - László, Bettina Réka AU - Szabó, István AU - Tóth, Attila AU - László, Kristóf AU - Győrfi, Nina Rubina AU - Lénárd, László AU - Karádi, Zoltán György TI - Different aspects of the taste reactivity test to interpret gustatory responses elicited organismic changes. T2 - 7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet PY - 2024 SP - 48 UR - https://m2.mtmt.hu/api/publication/34735416 ID - 34735416 LA - English DB - MTMT ER - TY - CONF AU - Hormay, Edina AU - Mintál, Kitti AU - László, Bettina Réka AU - Szabó, István AU - Tóth, Attila AU - László, Kristóf AU - Győrfi, Nina Rubina AU - Lénárd, László AU - Karádi, Zoltán György TI - Long term saccharose consumption associated activity of limbic forebrain glucose-monitoring neurons: A pilot study in the rat. T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - 129 UR - https://m2.mtmt.hu/api/publication/34735333 ID - 34735333 LA - English DB - MTMT ER -