TY - JOUR AU - Pöstyéni, Etelka AU - Szabadfi, Krisztina AU - Sétáló, György (ifj.) AU - Gábriel, Róbert TI - A promising combination: Pacap and parp inhibitor have therapeutic potential in models of diabetic and hypertensive retinopathies JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 12 PG - 18 SN - 2073-4409 DO - 10.3390/cells10123470 UR - https://m2.mtmt.hu/api/publication/32546342 ID - 32546342 N1 - Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs, 7624, Hungary Department of Medical Biology, Medical School, University of Pécs, Pécs, 7624, Hungary Cited By :4 Export Date: 24 October 2023 Correspondence Address: Gabriel, R.; Department of Experimental Zoology and Neurobiology, Hungary; email: gabriel@ttk.pte.hu LA - English DB - MTMT ER - TY - JOUR AU - Tékus, Valéria AU - Horváth, Ádám István AU - Csekő, Kata AU - Szabadfi, Krisztina AU - Kovács-Valasek, Andrea AU - Dányádi, Bese AU - Deres, László AU - Halmosi, Róbert AU - Sághy, Éva AU - Varga, Zoltán AU - Adeghate, Ernest Akingunola AU - Kőszegi, Tamás AU - Mátyus, Péter AU - Gábriel, Róbert AU - Ferdinandy, Péter AU - Pintér, Erika AU - Helyes, Zsuzsanna TI - Protective effects of the novel amine-oxidase inhibitor multi-target drug SZV 1287 on streptozotocin-induced beta cell damage and diabetic complications in rats JF - BIOMEDICINE & PHARMACOTHERAPY J2 - BIOMED PHARMACOTHER VL - 134 PY - 2021 PG - 16 SN - 0753-3322 DO - 10.1016/j.biopha.2020.111105 UR - https://m2.mtmt.hu/api/publication/31788019 ID - 31788019 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Sükösd, Andrea Krisztina AU - Szabadfi, Krisztina AU - Szabó-Meleg, Edina AU - Gáspár, B. AU - Stodulka, P. AU - Sétáló, György (ifj.) AU - Gábriel, Róbert AU - Nyitrai, Miklós AU - Biró, Zsolt AU - Ábrahám, Hajnalka TI - Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy JF - INTERNATIONAL JOURNAL OF OPHTHALMOLOGY (ENGLISH EDITION) J2 - INT J OPHTHALMOL (ENGLISH EDITION) VL - 13 PY - 2020 IS - 6 SP - 927 EP - 934 PG - 8 SN - 2222-3959 DO - 10.18240/ijo.2020.06.11 UR - https://m2.mtmt.hu/api/publication/31356167 ID - 31356167 LA - English DB - MTMT ER - TY - JOUR AU - Borbély, Éva AU - Kiss, Tamás AU - Szabadfi, Krisztina AU - Pintér, Erika AU - Szolcsányi, János AU - Helyes, Zsuzsanna AU - Botz, Bálint TI - Complex Role of Capsaicin-Sensitive Afferents in the Collagen Antibody-Induced Autoimmune Arthritis of the Mouse JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 8 ET - 0 PY - 2018 IS - 1 PG - 8 SN - 2045-2322 DO - 10.1038/s41598-018-34005-6 UR - https://m2.mtmt.hu/api/publication/30306941 ID - 30306941 N1 - * Megosztott szerzőség AB - Capsaicin-sensitive afferents have complex regulatory functions in the joints orchestrated via neuropeptides. This study aimed to determine their role in the collagen-antibody induced rheumatoid arthritis model. Capsaicin-sensitive nerves were defunctionalized by the capsaicin receptor agonist resiniferatoxin in C57Bl/6 mice. Arthritis was induced by the ArithroMab antibody cocktail and adjuvant. Arthritis was monitored by measuring body weight, joint edema by plethysmometry, arthritis severity by clinical scoring, mechanonociceptive threshold by plantar esthesiometry, thermonociceptive threshold by hot plate, cold tolerance by paw withdrawal latency from 0 °C water. Grasping ability was determined by the wire-grid grip test. Bone structure was evaluated by in vivo micro-CT and histology. Arthritic animals developed a modest joint edema, mechanical and cold hyperalgesia, weight loss, and a diminished grasping function, while thermal hyperalgesia is absent in the model. Desensitised mice displayed reduced arthritis severity, edema, and mechanical hyperalgesia, however, cold hyperalgesia was significantly greater in this group. Arthritic controls displayed a transient decrease of bone volume and an increased porosity, while bone density and trabecularity increased in desensitised mice. The activation of capsaicin-sensitive afferents increases joint inflammation and mechanical hyperalgesia, but decreases cold allodynia. It also affects inflammatory bone structural changes by promoting bone resorption. LA - English DB - MTMT ER - TY - JOUR AU - Kovács-Valasek, Andrea AU - Szabadfi, Krisztina AU - Dénes, Viktória AU - Szalontai, Bálint AU - Tamás, Andrea AU - Kiss, Péter AU - Szabó, Aliz AU - Sétáló, György (ifj.) AU - Reglődi, Dóra AU - Gábriel, Róbert TI - Accelerated retinal aging in PACAP knock-out mice. JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 348 PY - 2017 SP - 1 EP - 10 PG - 10 SN - 0306-4522 DO - 10.1016/j.neuroscience.2017.02.003 UR - https://m2.mtmt.hu/api/publication/3192281 ID - 3192281 AB - Pituitary adenylate cyclase activating polypeptide (PACAP) is a neurotrophic and neuroprotective peptide. PACAP and its receptors are widely distributed in the retina. A number of reports provided evidence that PACAP is neuroprotective in retinal degenerations. The current study compared retina cell type-specific differences in young (3-4months) and aged adults (14-16months), of wild-type (WT) mice and knock-out (KO) mice lacking endogenous PACAP production during the course of aging. Histological, immunocytochemical and Western blot examinations were performed. The staining for standard neurochemical markers (tyrosine hydroxylase for dopaminergic cells, calbindin 28 kDa for horizontal cells, protein kinase Calpha for rod bipolar cells) of young adult PACAP KO retinas showed no substantial alterations compared to young adult WT retinas, except for the specific PACAP receptor (PAC1-R) staining. We could not detect PAC1-R immunoreactivity in bipolar and horizontal cells in young adult PACAP KO animals. Some other age-related changes were observed only in the PACAP KO mice only. These alterations included horizontal and rod bipolar cell dendritic sprouting into the photoreceptor layer and decreased ganglion cell number. Also, Muller glial cells showed elevated GFAP expression compared to the aging WT retinas. Furthermore, Western blot analyses revealed significant differences between the phosphorylation state of ERK1/2 and JNK in KO mice, indicating alterations in the MAPK signaling pathway. These results support the conclusion that endogenous PACAP contributes to protection against aging of the nervous system. LA - English DB - MTMT ER - TY - JOUR AU - Szabadfi, Krisztina AU - Reglődi, Dóra AU - Szabó, Aliz AU - Szalontai, Bálint AU - Kovács-Valasek, Andrea AU - Sétáló, György (ifj.) AU - Kiss, Péter AU - Tamás, Andrea AU - Wilhelm, Márta AU - Gábriel, Róbert TI - Pituitary Adenylate Cyclase Activating Polypeptide, A Potential Therapeutic Agent for Diabetic Retinopathy in Rats: Focus on the Vertical Information Processing Pathway JF - NEUROTOXICITY RESEARCH J2 - NEUROTOX RES VL - 29 PY - 2016 IS - 3 SP - 432 EP - 446 PG - 15 SN - 1029-8428 DO - 10.1007/s12640-015-9593-1 UR - https://m2.mtmt.hu/api/publication/2994283 ID - 2994283 LA - English DB - MTMT ER - TY - JOUR AU - Hajna, Zsófia Réka AU - Szabadfi, Krisztina AU - Balla, Z AU - Biró, Zsolt AU - Degrell, P AU - Molnár, Gergő Attila AU - Kőszegi, Tamás AU - Tékus, Valéria AU - Helyes, Zsuzsanna AU - Dobos, András AU - Farkas, S AU - Szűcs, Gyula AU - Gábriel, Róbert AU - Pintér, Erika TI - Modeling long-term diabetes and related complications in rats JF - JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS J2 - J PHARMACOL TOXICOL METH VL - 78 PY - 2016 SP - 1 EP - 12 PG - 12 SN - 1056-8719 DO - 10.1016/j.vascn.2015.11.003 UR - https://m2.mtmt.hu/api/publication/2977092 ID - 2977092 N1 - * Megosztott szerzőség AB - INTRODUCTION: Accurate preclinical modeling of diabetic complications such as retinopathy, nephropathy and neuropathy is crucial to enable the development of novel preventative therapies. The aims of this study were to establish a model of long-term diabetes with sustained medium scale hyperglycemia and characterize the pathological changes detectable after 4months, with particular respect to dependence on the degree of hyperglycemia. METHODS: Streptozotocin-induced diabetic CFY rats were subjected to four different insulin substitution protocols to achieve different levels of glycemic control (Diabetic 1-4 groups). Eyes were investigated by ophthalmoscopy, kidney function by urine analysis, and neuropathy by functional tests. Retinal and renal morphological evaluations were performed by histology, immuno-histochemistry and electronmicroscopy. RESULTS: Rats of the Diabetic 3 group showed massive hyperglycemia-dependent anterior segment neovascularization, enhanced total retinal score and retinal apoptotic cell number, degeneration of dopaminergic amacrine cells, increased glomerular PAS-positivity, altered excreted total protein/creatinine ratio and cold allodynia, beside medium scale hyperglycemia (blood glucose level between 22 and 25mmol/L) and acceptable health status of the animals parallelly. DISCUSSION: We established a treatment protocol in rats enabling complex investigation of diabetic retinopathy, nephropathy and neuropathy on a long-term period. Clearly hyperglycemic dependent parameters of these complications serve as good outcome measures for preclinical trials. Our results provide a useful basis for designing studies for testing preventative treatments as well as other translational medical research in this field. LA - English DB - MTMT ER - TY - JOUR AU - Helyes, Zsuzsanna AU - Kun, József AU - Dobrosi, Nóra AU - Sándor, Katalin AU - Németh, J AU - Perkecz, A AU - Pintér, Erika AU - Szabadfi, Krisztina AU - Gaszner, Balázs AU - Tékus, Valéria AU - Szolcsányi, János AU - Steinhoff, M AU - Hashimoto, H AU - Reglődi, Dóra AU - Bíró, Tamás TI - Pituitary Adenylate Cyclase-Activating Polypeptide Is Upregulated in Murine Skin Inflammation and Mediates Transient Receptor Potential Vanilloid-1-Induced Neurogenic Edema JF - JOURNAL OF INVESTIGATIVE DERMATOLOGY J2 - J INVEST DERMATOL VL - 135 PY - 2015 IS - 9 SP - 2209 EP - 2218 PG - 10 SN - 0022-202X DO - 10.1038/jid.2015.156 UR - https://m2.mtmt.hu/api/publication/2914853 ID - 2914853 N1 - Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Pécs, Szigeti u. 12, Pécs, 7624, Hungary János Szentágothai Research Center, University of Pécs, Pécs, Hungary PharmInVivo Ltd., Pécs, Hungary MTA NAP B Pain Research Group, University of Pécs, School of Medicine, Pécs, Hungary DE-MTA Lendület Cellular Physiology Research Group, Departments of Physiology and Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Pharmacology and Pharmacotherapy, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary Department of Experimental Zoology and Neurobiology, Faculty of Sciences, University of Pecs, Pecs, Hungary Department of Anatomy, MTA-PTE PACAP Lendület Research Group, Faculty of Medicine, University of Pécs, Pécs, Hungary Department of Dermatology, Charles Clinic and UCD Charles Institute for Translational Dermatology, University College Dublin, Dublin, Ireland Lab. of Molec. Neuropharmacology, IPS Cell-based Res. Proj. on Brain Neuropharmacology and Toxicol., Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Japan United Graduate School of Child Development, Osaka University, Suita, Japan Cited By :13 Export Date: 19 September 2023 CODEN: JIDEA Correspondence Address: Helyes, Z.; Department of Pharmacology and Pharmacotherapy, Szigeti u. 12, Hungary; email: zsuzsanna.helyes@aok.pte.hu LA - English DB - MTMT ER - TY - JOUR AU - Szabadfi, Krisztina AU - Estrada, C AU - Fernandez-Villalba, E AU - Tarragon, E AU - Sétáló, György (ifj.) AU - Izura, V AU - Reglődi, Dóra AU - Tamás, Andrea AU - Gábriel, Róbert AU - Herrero, MT TI - Retinal aging in the diurnal Chilean rodent (Octodon degus): histological, ultrastructural and neurochemical alterations of the vertical information processing pathway JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 9 PY - 2015 PG - 14 SN - 1662-5102 DO - 10.3389/fncel.2015.00126 UR - https://m2.mtmt.hu/api/publication/2867789 ID - 2867789 AB - BACKGROUND: The retina is sensitive to age-dependent degeneration. To find suitable animal models to understand and map this process has particular importance. The degu (Octodon degus) is a diurnal rodent with dichromatic color vision. Its retinal structure is similar to that in humans in many respects, therefore, it is well suited to study retinal aging. Histological, cell type-specific and ultrastructural alterations were examined in 6, 12 and 36 months old degus. The characteristic layers of the retina were present at all ages, but slightly loosened tissue structure could be observed in 36-month-old animals both at light and electron microscopic levels. Elevated glial fibrillary acidic protein expression was observed in Müller glial cells in aging retinas. The number of rod bipolar cells and the ganglion cells was reduced in the aging specimens, while that of cone bipolar cells remained unchanged. Other age-related differences were detected at ultrastructural level: alteration of the retinal pigment epithelium and degenerated photoreceptor cells were evident. Ribbon synapses were sparse and often differed in morphology from those in the young animals. These results support our hypothesis that (i) the rod pathway seems to be more sensitive than the cone pathway to age-related cell loss; (ii) structural changes in the basement membrane of pigment epithelial cells can be one of the early signs of degenerative processes; (iii) the loss of synaptic proteins especially from those of the ribbon synapses are characteristic and (iv) the degu retina may be a suitable model for studying retinal aging. LA - English DB - MTMT ER - TY - CONF AU - Szabadfi, Krisztina AU - Gábriel, Róbert AU - Ifj., Sétáló György AU - Tarragon, E. AU - Estrada, C. AU - Lopez, D. AU - Ros, C.M. AU - Reglődi, Dóra AU - Tamás, Andrea AU - Herrero, M.T. TI - A Chilean rodent, Octodon degus is a promising model for studying retinal aging [poster] T2 - 9th FENS Forum of Neuroscience PY - 2014 SP - x UR - https://m2.mtmt.hu/api/publication/2781369 ID - 2781369 LA - English DB - MTMT ER -