@article{MTMT:2525803, title = {Monitoring Repair of UV-Induced 6-4-Photoproducts with a Purified DDB2 Protein Complex.}, url = {https://m2.mtmt.hu/api/publication/2525803}, author = {Dreze, M and Calkins, AS and Gálicza, Judit and Echelman, DJ and Schnorenberg, MR and Fell, GL and Iwai, S and Fisher, DE and Szüts, Dávid and Iglehart, JD and Lazaro, JB}, doi = {10.1371/journal.pone.0085896}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {9}, unique-id = {2525803}, issn = {1932-6203}, abstract = {Because cells are constantly subjected to DNA damaging insults, DNA repair pathways are critical for genome integrity [1]. DNA damage recognition protein complexes (DRCs) recognize DNA damage and initiate DNA repair. The DNA-Damage Binding protein 2 (DDB2) complex is a DRC that initiates nucleotide excision repair (NER) of DNA damage caused by ultraviolet light (UV) [2]-[4]. Using a purified DDB2 DRC, we created a probe ("DDB2 proteo-probe") that hybridizes to nuclei of cells irradiated with UV and not to cells exposed to other genotoxins. The DDB2 proteo-probe recognized UV-irradiated DNA in classical laboratory assays, including cyto- and histo-chemistry, flow cytometry, and slot-blotting. When immobilized, the proteo-probe also bound soluble UV-irradiated DNA in ELISA-like and DNA pull-down assays. In vitro, the DDB2 proteo-probe preferentially bound 6-4-photoproducts [(6-4)PPs] rather than cyclobutane pyrimidine dimers (CPDs). We followed UV-damage repair by cyto-chemistry in cells fixed at different time after UV irradiation, using either the DDB2 proteo-probe or antibodies against CPDs, or (6-4)PPs. The signals obtained with the DDB2 proteo-probe and with the antibody against (6-4)PPs decreased in a nearly identical manner. Since (6-4)PPs are repaired only by nucleotide excision repair (NER), our results strongly suggest the DDB2 proteo-probe hybridizes to DNA containing (6-4)PPs and allows monitoring of their removal during NER. We discuss the general use of purified DRCs as probes, in lieu of antibodies, to recognize and monitor DNA damage and repair.}, year = {2014}, eissn = {1932-6203} } @article{MTMT:2501652, title = {Internalization and cytotoxicity assays of transferrin complexes into stem cell-like glioblastoma cells}, url = {https://m2.mtmt.hu/api/publication/2501652}, author = {Gálicza, Judit and Éva, Fischer-Fodor and Takácsi-Nagy, Anna Erzsébet and Vargová, Andrea and Albert, Beáta and Lányi, Szabolcs and Kilár, Ferenc}, journal-iso = {STUD UNIV BABES-BOLYAI CHEM}, journal = {STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA}, volume = {2013}, unique-id = {2501652}, issn = {1224-7154}, year = {2013}, eissn = {2065-9520}, pages = {33-42}, orcid-numbers = {Takácsi-Nagy, Anna Erzsébet/0000-0002-4756-9136; Kilár, Ferenc/0000-0001-5737-5514} } @article{MTMT:2501649, title = {Separation optimization of the molecular forms of transferrin complexes by capillary zone electrohoresis}, url = {https://m2.mtmt.hu/api/publication/2501649}, author = {Gálicza, Judit and Sándor, Viktor and Vargová, Andrea and Albert, Beáta and Lányi, Szabolcs and Kilár, Ferenc}, journal-iso = {UPB SCI BULL SER B}, journal = {UPB SCIENTIFIC BULLETIN, SERIES B: CHEMISTRY AND MATERIALS SCIENCE}, volume = {74}, unique-id = {2501649}, issn = {1454-2331}, year = {2012}, pages = {153-164}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} } @article{MTMT:1782601, title = {Preparation and Investigation of Bioactive Transferrin-Iron Complexes Formed with Different Synergistic Anions}, url = {https://m2.mtmt.hu/api/publication/1782601}, author = {Gálicza, Judit and Vargová, Andrea and Sándor, Viktor and Orbán, Kálmán Csongor and András, Csaba Dezső and Albert, Beáta and Lányi, Szabolcs and Kilár, Ferenc}, doi = {10.1007/s10930-011-9370-9}, journal-iso = {PROTEIN J}, journal = {PROTEIN JOURNAL}, volume = {31}, unique-id = {1782601}, issn = {1572-3887}, year = {2012}, eissn = {1573-4943}, pages = {27-34}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} } @article{MTMT:2501643, title = {Conus magus vs. Irukandji syndrome: A computational approach of a possible new therapy}, url = {https://m2.mtmt.hu/api/publication/2501643}, author = {András, Csaba Dezső and Albert, Csilla and Szidónia, Salamon and Gálicza, Judit and Réka, András and Emil, András}, doi = {10.1016/j.brainresbull.2011.07.003}, journal-iso = {BRAIN RES BULL}, journal = {BRAIN RESEARCH BULLETIN}, volume = {86}, unique-id = {2501643}, issn = {0361-9230}, abstract = {The Irukandji syndrome is caused by the sting of some small jellyfish species. The syndrome has severe life-threatening consequences. The exacerbating pain and cardiovascular symptoms (tachycardia and hypertension) are hard to control in many cases. We suggest a way to experiment a new possible therapy with an FDA approved analgesic, ziconotide, a synthetic derivative from a marine cone snail (Conus magus) venom component, which is administrated intravenously. The proposed experimental plasma concentration of ziconotide for rats is in the range of 0-6μgml -1. Based on a molecular biological scenario of the venom action mechanism at cellular level, we suggest that the proposed method should be functional in re-establishing the normal cardiovascular parameters of the experimental animals and concomitantly it should abolish the severe pain caused by envenomation. We expect that positive experimental results in agreement with our theory will lead to the possibility of a new therapy for the Irukandji syndrome and possibly for other envenomations with similar ethyology.}, year = {2011}, eissn = {1873-2747}, pages = {195-202}, orcid-numbers = {Albert, Csilla/0000-0001-6882-6691} } @article{MTMT:2501655, title = {Modeling studies on the ancestral proteinase stemzyme IDP-B}, url = {https://m2.mtmt.hu/api/publication/2501655}, author = {László, Szilágyi and Albert, Beáta and Miklóssy, Ildikó and Szabolcs, Szilveszter and Gálicza, Judit and Lányi, Szabolcs}, journal-iso = {UPB SCI BULL SER B}, journal = {UPB SCIENTIFIC BULLETIN, SERIES B: CHEMISTRY AND MATERIALS SCIENCE}, volume = {72}, unique-id = {2501655}, issn = {1454-2331}, year = {2010}, pages = {161-168} } @article{MTMT:2225334, title = {Modeling studies on the ancestral proteinase stemzyme IDP-Β}, url = {https://m2.mtmt.hu/api/publication/2225334}, author = {Szilágyi, L and Albert, Beáta and Miklóssy, Ildikó and Szilveszter, Szabolcs and Gálicza, Judit and Lányi, Szabolcs}, journal-iso = {SCI BULL POLYTECH INST BUCHAR CHEM MATER SCI}, journal = {SCIENTIFIC BULLETIN POLYTECHNIC INSTITUTE OF BUCHAREST CHEMISTRY AND MATERIALS SCIENCE}, volume = {72}, unique-id = {2225334}, issn = {1220-305X}, year = {2010}, pages = {161-168} } @article{MTMT:2172239, title = {Preparation and modelling of the structure of transferrin-Fe3+-aziridine-carboxylate complex}, url = {https://m2.mtmt.hu/api/publication/2172239}, author = {Gálicza, Judit and Orbán, Kálmán Csongor and Kilár, Ferenc and Miklóssy, Ildikó and Albert, Beáta and Lányi, Szabolcs}, journal-iso = {STUD UNIV BABES-BOLYAI CHEM}, journal = {STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA}, volume = {54}, unique-id = {2172239}, issn = {1224-7154}, year = {2010}, eissn = {2065-9520}, pages = {45-52}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} } @{MTMT:1731569, title = {Preparation and investigation of bioactive transferrin-iron complexes formed with different synergistic anions}, url = {https://m2.mtmt.hu/api/publication/1731569}, author = {Gálicza, Judit and Vargová, Andrea and Sándor, Viktor and Páger, Csilla and Fodor, Rita and Miklóssy, Ildikó and Albert, Beáta and Lányi, Szabolcs and Kilár, Ferenc}, booktitle = {25th International Symposium on Microscale BioSeparations MSB 2010}, unique-id = {1731569}, year = {2010}, pages = {181-181}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} } @article{MTMT:1423615, title = {Rákos sejtekbe történő, transzferrinen alapuló hatóanyag bejuttatás}, url = {https://m2.mtmt.hu/api/publication/1423615}, author = {Gálicza, Judit and Vargová, Andrea and Sándor, Viktor and Kilár, Ferenc and Miklóssy, Ildikó and Albert, Beáta and Lányi, Szabolcs}, journal-iso = {CSÍKI SZÉKELY MÚZ ÉVK}, journal = {A CSÍKI SZÉKELY MÚZEUM ÉVKÖNYVE}, volume = {6}, unique-id = {1423615}, issn = {1841-0197}, year = {2010}, pages = {479-487}, orcid-numbers = {Kilár, Ferenc/0000-0001-5737-5514} }