TY - JOUR AU - Jakabfi-Csepregi, Rita AU - Alberti, Ágnes AU - Felegyi-Tóth, Csenge Anna AU - Kőszegi, Tamás AU - Czigle, Szilvia AU - Papp, Nóra TI - A Comprehensive Study on Lathyrus tuberosus L.: Insights into Phytochemical Composition, Antimicrobial Activity, Antioxidant Capacity, Cytotoxic, and Cell Migration Effects JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 13 PY - 2024 IS - 2 PG - 29 SN - 2223-7747 DO - 10.3390/plants13020232 UR - https://m2.mtmt.hu/api/publication/34503796 ID - 34503796 AB - In this study, in vitro antioxidant, antimicrobial, cytotoxic, and cell migration effects of phenolic compounds of Lathyrus tuberosus leaves, known in the Transylvanian ethnomedicine, were investigated. Ultra-high-performance liquid chromatography-tandem mass spectrometry was employed for the analysis of the ethanolic and aqueous extracts. The antimicrobial properties were determined using a conventional microdilution technique. Total antioxidant capacity techniques were used using cell-free methods and cell-based investigations. Cytotoxic effects were conducted on 3T3 mouse fibroblasts and HaCaT human keratinocytes using a multiparametric method, assessing intracellular ATP, total nucleic acid, and protein levels. Cell migration was visualized by phase-contrast microscopy, employing conventional culture inserts to make cell-free areas. Together, 93 polyphenolic and monoterpenoid compounds were characterized, including flavonoid glycosides, lignans, hydroxycinnamic acid, and hydroxybenzoic acid derivatives, as well as iridoids and secoiridoids. The ethanolic extract showed high antioxidant capacity and strong antimicrobial activity against Bacillus subtilis (MIC80 value: 354.37 ± 4.58 µg/mL) and Streptococcus pyogenes (MIC80 value: 488.89 ± 4.75 µg/mL). The abundance of phenolic compounds and the results of biological tests indicate the potential for L. tuberosus to serve as reservoirs of bioactive compounds and to be used in the development of novel nutraceuticals. LA - English DB - MTMT ER - TY - JOUR AU - Kurdi, Csilla AU - Schmidt, János AU - Horváth-Szalai, Zoltán AU - Mauchart, Péter AU - Gödöny, Krisztina AU - Várnagy, Ákos AU - Kovács, L. Gábor AU - Kőszegi, Tamás TI - Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 1 PG - 15 SN - 1661-6596 DO - 10.3390/ijms25010486 UR - https://m2.mtmt.hu/api/publication/34486045 ID - 34486045 N1 - János Szentágothai Research Center, University of Pécs, Pécs, 7624, Hungary Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, 7624, Hungary National Laboratory on Human Reproduction, University of Pécs, Pécs, 7624, Hungary Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Pécs, 7624, Hungary Department of Obstetrics and Gynecology, Medical School, University of Pécs, Pécs, 7624, Hungary MTA-PTE Human Reproduction Scientific Research Group, University of Pécs, Pécs, 7624, Hungary Export Date: 22 January 2024 Correspondence Address: Kőszegi, T.; János Szentágothai Research Center, Hungary; email: koszegi.tamas@pte.hu AB - Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility, and this fluid is a critical microenvironment for the developing oocytes as well. Using the high-performance liquid chromatography-mass spectrometry method, we investigated the protein composition of the follicular fluid, and after classification, we carried out relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein–protein interaction analysis, albumin and apolipoprotein A1 (ApoA1) were found to be hub proteins, and the quantitative comparison of the P and NP groups resulted in a significantly lower concentration of ApoA1 and high-density lipoprotein cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility. LA - English DB - MTMT ER - TY - JOUR AU - Szirmay, Balázs AU - Horváth-Szalai, Zoltán AU - Ludány, Andrea AU - Miseta, Attila János AU - Wittmann, István AU - Kőszegi, Tamás TI - THE DIAGNOSTIC AND PROGNOSTIC ROLE OF URINARY PROTEIN MARKERS IN PATIENTS WITH SARS-COV-2 INFECTION JF - CLINICAL CHEMISTRY AND LABORATORY MEDICINE J2 - CLIN CHEM LAB MED VL - 61 PY - 2023 IS - s1 SP - s738 EP - s738 PG - 1 SN - 1434-6621 UR - https://m2.mtmt.hu/api/publication/34449761 ID - 34449761 LA - English DB - MTMT ER - TY - JOUR AU - Vass, Réka Anna AU - Mikó, Éva AU - Gál, Csenge AU - Kőszegi, Tamás AU - Vass, Csaba I AU - Bokor, Szilvia AU - Molnár, Dénes AU - Funke Kiss, Simone AU - Kovács, Kálmán András AU - Bódis, József AU - Ertl, Tibor TI - The Effect of Holder Pasteurization and Different Variants on Breast Milk Antioxidants JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 12 PY - 2023 IS - 10 PG - 13 SN - 2076-3921 DO - 10.3390/antiox12101857 UR - https://m2.mtmt.hu/api/publication/34227655 ID - 34227655 N1 - ISSN:2076-3921 AB - After birth, breast milk (BM) is a known essential source of antioxidants for infants. We analyzed the non-enzymatic total antioxidant capacity (TAC), oxygen radical absorbance capacity (ORAC), and glutathione, calcium, transferrin, and total protein levels of human breast milk before and after Holder pasteurization (HoP).The collected donor BM samples were pasteurized with HoP.HoP decreased TAC (-12.6%), ORAC (-12.1%), transferrin (-98.3%), and total protein (-21.4%) levels; HoP did not influence the glutathione concentration, and it increased the total calcium (+25.5%) concentration. Mothers who gave birth via Cesarean section had significantly lower TAC in their BM. TAC and glutathione levels were elevated in the BM of mothers over the age of 30. BM produced in the summer had higher glutathione and calcium levels compared to BM produced in the winter. The glutathione concentration in term milk samples was significantly higher in the first two months of lactation compared to the period between the third and sixth months. The transferrin level of BM for female infants was significantly higher than the BM for boys, and mothers with a BMI above 30 had increased transferrin in their samples.Antioxidant levels in human milk are influenced by numerous factors. Environmental and maternal factors, the postpartum age at breast milk collection, and Holder pasteurization of the milk influence the antioxidant intake of the infant. LA - English DB - MTMT ER - TY - JOUR AU - Kőszegi, Tamás AU - Horváth-Szalai, Zoltán AU - Ragán, Dániel AU - Kósa, Brigitta AU - Szirmay, Balázs AU - Kurdi, Csilla AU - Kovács, L. Gábor AU - Mühl, Diána TI - Measurement of Urinary Gc-Globulin by a Fluorescence ELISA Technique: Method Validation and Clinical Evaluation in Septic Patients—A Pilot Study JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 19 PG - 15 SN - 1420-3049 DO - 10.3390/molecules28196864 UR - https://m2.mtmt.hu/api/publication/34167218 ID - 34167218 N1 - Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, 7624, Hungary János Szentágothai Research Center, University of Pécs, Pécs, 7624, Hungary Hungarian National Laboratory on Reproduction, University of Pécs, Pécs, 7624, Hungary Department of Anesthesiology and Intensive Therapy, Medical School, University of Pécs, Pécs, 7624, Hungary Export Date: 13 November 2023 CODEN: MOLEF Correspondence Address: Kőszegi, T.; Department of Laboratory Medicine, Hungary; email: koszegi.tamas@pte.hu Correspondence Address: Horváth-Szalai, Z.; Department of Laboratory Medicine, Hungary; email: horvath-szalai.zoltan@pte.hu AB - A major complication of sepsis is the development of acute kidney injury (AKI). In case of acute tubular damage, Gc-globulin, a known serum sepsis marker is increasingly filtrated into the urine therefore, urinary Gc-globulin (u-Gc) levels may predict septic AKI. We developed and validated a competitive fluorescence ELISA method for u-Gc measurement. Serum and urine samples from septic patients were collected in three consecutive days (T1, T2, T3) and data were compared to controls. Intra- and interassay imprecisions were CV < 14% and CV < 20%, respectively, with a recovery close to 100%. Controls and septic patients differed (p < 0.001) in their u-Gc/u-creatinine levels at admission (T1, median: 0.51 vs. 79.1 µg/mmol), T2 (median: 0.51 vs. 57.8 µg/mmol) and T3 (median: 0.51 vs. 55.6 µg/mmol). Septic patients with AKI expressed higher u-Gc/u-creatinine values than those without AKI at T1 (median: 23.6 vs. 136.5 µg/mmol, p < 0.01) and T3 (median: 34.4 vs. 75.8 µg/mmol, p < 0.05). AKI-2 stage patients exhibited more increased u-Gc/u-creatinine levels at T1 (median: 207.1 vs. 53.3 µg/mmol, p < 0.05) than AKI-1 stage individuals. Moderate correlations (p < 0.001) were observed between u-Gc/u-creatinine and se-urea, se-creatinine, se-hsCRP, WBC, u-total protein, u-albumin, u-orosomucoid/u-creatinine, and u-Cystatin C/u-creatinine levels. U-Gc testing may have a predictive value for AKI in septic patients. LA - English DB - MTMT ER - TY - JOUR AU - Horváth-Szalai, Zoltán AU - Jakabfi-Csepregi, Rita AU - Szirmay, Balázs AU - Ragán, Dániel AU - Simon, Gerda AU - Kovács-Ábrahám, Zoltán AU - Szabó, Péter AU - Sipos, Dávid AU - Péterfalvi, Ágnes AU - Miseta, Attila János AU - Csontos, Csaba AU - Kőszegi, Tamás AU - Némethné Tóth, Ildikó TI - Serum Total Antioxidant Capacity (TAC) and TAC/Lymphocyte Ratio as Promising Predictive Markers in COVID-19 JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 16 PG - 14 SN - 1661-6596 DO - 10.3390/ijms241612935 UR - https://m2.mtmt.hu/api/publication/34113082 ID - 34113082 N1 - ISSN:1422-0067 AB - SARS-CoV-2 infection might cause a critical disease, and patients' follow-up is based on multiple parameters. Oxidative stress is one of the key factors in the pathogenesis of COVID-19 suggesting that its level could be a prognostic marker. Therefore, we elucidated the predictive value of the serum non-enzymatic total antioxidant capacity (TAC) and that of the newly introduced TAC/lymphocyte ratio in COVID-19. We included 61 COVID-19 (n = 27 ward, n = 34 intensive care unit, ICU) patients and 29 controls in our study. Serum TAC on admission was measured by an enhanced chemiluminescence (ECL) microplate assay previously validated by our research group. TAC levels were higher (p < 0.01) in ICU (median: 407.88 µmol/L) than in ward patients (315.44 µmol/L) and controls (296.60 µmol/L). Besides the classical parameters, both the TAC/lymphocyte ratio and TAC had significant predictive values regarding the severity (AUC-ROC for the TAC/lymphocyte ratio: 0.811; for TAC: 0.728) and acute kidney injury (AUC-ROC for the TAC/lymphocyte ratio: 0.747; for TAC: 0.733) in COVID-19. Moreover, the TAC/lymphocyte ratio had significant predictive value regarding mortality (AUC-ROC: 0.752). Serum TAC and the TAC/lymphocyte ratio might offer valuable information regarding the severity of COVID-19. TAC measured by our ECL microplate assay serves as a promising marker for the prediction of systemic inflammatory diseases. LA - English DB - MTMT ER - TY - JOUR AU - Kurdi, Csilla AU - Lelovics, Vanessza AU - Hesszenberger, Dávid AU - Lajtai, Anikó AU - Lakatos, Ágnes AU - Herczeg, Róbert AU - Gödöny, Krisztina AU - Mauchart, Péter AU - Várnagy, Ákos AU - Kovács, L. Gábor AU - Kőszegi, Tamás TI - Amino Acid Profiling of Follicular Fluid in Assisted Reproduction Reveals Important Roles of Several Amino Acids in Patients with Insulin Resistance JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 15 SN - 1661-6596 DO - 10.3390/ijms241512458 UR - https://m2.mtmt.hu/api/publication/34106678 ID - 34106678 N1 - Funding Agency and Grant Number: National Laboratory on Human Reproduction" program [RRF-2.3.1-21-2022-00012]; University of Pecs, Hungary [EFOP 3.6.1-16-2016-00004] Funding text: The research was financed by the RRF-2.3.1-21-2022-00012 "National Laboratory on Human Reproduction" program and by the EFOP 3.6.1-16-2016-00004 project (Comprehensive Development for Implementing Smart Specialization Strategies), University of Pecs, Hungary. AB - The global prevalence of insulin resistance (IR) is increasing continuously, influencing metabolic parameters and fertility. The metabolic changes due to IR can alter the molecular composition of plasma and other body fluids. Follicular fluid (FF) is derived mainly from plasma, and it is a critical microenvironment for the developing oocytes. It contains various metabolites and amino acids, and the quality of the oocytes is linked at least partially to amino acid metabolism. Our goal was to quantitatively determine the amino acid (AA) profile of FF in IVF patients and to compare IR and non-insulin resistance (NIR) groups to investigate the AA changes in their FF. Using UHPLC-based methods, we quantified the main 20 amino acids from human FF samples in the IR and NIR groups. Several amino acids (aspartate, glycine, glutamate, and cysteine) differed significantly (p < 0.05 or less) between the two groups. The most significant alterations between the IR and NIR groups were related to the glutathione metabolic pathway involving glycine, serine, and threonine. Since insulin resistance alters the amino acid composition of the FF, the oocytes may undergo metabolism-induced changes resulting in poor oocyte quality and less fertility in the insulin resistance groups. LA - English DB - MTMT ER - TY - JOUR AU - Gergics, Marin AU - Pham-Dobor, Gréta AU - Kurdi, Csilla AU - Montskó, Gergely AU - Mihályi, Krisztina AU - Bánfai, Gábor László AU - Kanizsai, Péter László AU - Kőszegi, Tamás AU - Mezősi, Emese AU - Bajnok, László Zoltán TI - Apelin-13 as a Potential Biomarker in Critical Illness JF - JOURNAL OF CLINICAL MEDICINE J2 - J CLIN MED VL - 12 PY - 2023 IS - 14 PG - 11 SN - 2077-0383 DO - 10.3390/jcm12144801 UR - https://m2.mtmt.hu/api/publication/34081443 ID - 34081443 N1 - ISSN:2077-0383 AB - The adrenocortical system and copeptin as prognostic markers were intensively investigated in critical illness. The potential predictive power of apelin-13 as a biomarker is largely unknown. We aimed to investigate the prognostic role of apelin-13 in relation to free cortisol, aldosterone, CRH, and copeptin in critically ill patients.In this prospective observational study, 124 critically ill patients (64 men, 60 women, median age: 70 (59-78) years) were consecutively enrolled at the time of admission. All routinely available clinical and laboratory parameters were evaluated and correlated to hormonal changes.Serum apelin-13 was 1161 (617-2967) pg/mL in non-survivors vs. 2477 (800-3531) pg/mL in survivors (p = 0.054). The concentrations of apelin-13 and CRH had strong positive correlations (r = 0.685, p < 0.001) and were significantly higher in surviving non-septic patients (Apelin-13 (pg/mL): 2286 (790-3330) vs. 818 (574-2732) p < 0.05; CRH (pg/mL) 201 (84-317) vs. 89 (74-233) p < 0.05). Apelin-13 and free cortisol were independent determinants of survival in the multivariate Cox regression analysis, while copeptin, CRH, or aldosterone were not.Beyond free cortisol, serum apelin-13 may also help refine prognostic predictions in the early phase of critical illness, especially in non-septic patients. LA - English DB - MTMT ER - TY - JOUR AU - Sági, Balázs AU - Vas, Tibor AU - Jakabfi-Csepregi, Rita AU - Horváth-Szalai, Zoltán AU - Kőszegi, Tamás AU - Csiky, Botond AU - Nagy, Judit AU - Kovács, Tibor TI - The Role of Two Heart Biomarkers in IgA Nephropathy JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 12 PG - 11 SN - 1661-6596 DO - 10.3390/ijms241210336 UR - https://m2.mtmt.hu/api/publication/34036926 ID - 34036926 AB - Cardiovascular mortality is a leading cause of death in chronic kidney disease (CKD), as is IgA nephropathy (IgAN). The purpose of this study is to find different biomarkers to estimate the outcome of the disease, which is significantly influenced by the changes in vessels (characterized by arterial stiffness) and the heart. In our cross-sectional study, 90 patients with IgAN were examined. The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) was measured as a heart failure biomarker by an automated immonoassay method, while the carboxy-terminal telopeptide of collagen type I (CITP) as a fibrosis marker was determined using ELISA kits. Arterial stiffness was determined by measuring carotid–femoral pulse wave velocity (cfPWV). Renal function and routine echocardiography examinations were performed as well. Based on eGFR, patients were separated into two categories, CKD 1-2 and CKD 3-5. There were significantly higher NT-proBNP (p = 0.035), cfPWV (p = 0.004), and central aortic systolic pressure (p = 0.037), but not CITP, in the CKD 3-5 group. Both biomarker positivities were significantly higher in the CKD 3-5 group (p = 0.035) compared to the CKD 1-2 group. The central aortic systolic pressure was significantly higher in the diastolic dysfunction group (p = 0.034), while the systolic blood pressure was not. eGFR and hemoglobin levels showed a strong negative correlation, while left ventricular mass index (LVMI), aortic pulse pressure, central aortic systolic pressure, and cfPWV showed a positive correlation with NT-proBNP. cfPWV, aortic pulse pressure, and LVMI showed a strong positive correlation with CITP. Only eGFR was an independent predictor of NT-proBNP by linear regression analysis. NT-proBNP and CITP biomarkers may help to identify IgAN patients at high risk for subclinical heart failure and further atherosclerotic disease. LA - English DB - MTMT ER - TY - JOUR AU - Tyukodi, Levente AU - Zsidó, Balázs Zoltán AU - Hetényi, Csaba AU - Kőszegi, Tamás AU - Huber, Imre AU - Rozmer, Zsuzsanna TI - Serum albumin binding studies on antiproliferative cyclic C5-curcuminoid derivatives using spectroscopic methods and molecular modelling JF - JOURNAL OF MOLECULAR STRUCTURE J2 - J MOL STRUCT VL - 1287 PY - 2023 PG - 39 SN - 0022-2860 DO - 10.1016/j.molstruc.2023.135761 UR - https://m2.mtmt.hu/api/publication/33811185 ID - 33811185 N1 - Department of Pharmaceutical Chemistry, University of Pécs, 7624 Pécs, Rókus u. 2., Pécs, Hungary Department of Pharmacology and Pharmacotherapy, Unit of Pharmacoinformatics, University of Pécs, Pécs, Hungary National Laboratory for Drug Research and Development, Budapest, Hungary Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, Hungary János Szentágothai Research Centre, University of Pécs, Pécs, Hungary Export Date: 6 September 2023 CODEN: JMOSB Correspondence Address: Rozmer, Z.; Department of Pharmaceutical Chemistry, 7624 Pécs, Rókus u. 2., Hungary; email: rozmer.zsuzsanna@pte.hu LA - English DB - MTMT ER -