@article{MTMT:34503796,
	title = {A Comprehensive Study on Lathyrus tuberosus L.: Insights into Phytochemical Composition, Antimicrobial Activity, Antioxidant Capacity, Cytotoxic, and Cell Migration Effects},
	url = {https://m2.mtmt.hu/api/publication/34503796},
	author = {Jakabfi-Csepregi, Rita and Alberti, Ágnes and Felegyi-Tóth, Csenge Anna and Kőszegi, Tamás and Czigle, Szilvia and Papp, Nóra},
	doi = {10.3390/plants13020232},
	journal-iso = {PLANTS-BASEL},
	journal = {PLANTS-BASEL},
	volume = {13},
	unique-id = {34503796},
	abstract = {In this study, in vitro antioxidant, antimicrobial, cytotoxic, and cell migration effects of phenolic compounds of Lathyrus tuberosus leaves, known in the Transylvanian ethnomedicine, were investigated. Ultra-high-performance liquid chromatography-tandem mass spectrometry was employed for the analysis of the ethanolic and aqueous extracts. The antimicrobial properties were determined using a conventional microdilution technique. Total antioxidant capacity techniques were used using cell-free methods and cell-based investigations. Cytotoxic effects were conducted on 3T3 mouse fibroblasts and HaCaT human keratinocytes using a multiparametric method, assessing intracellular ATP, total nucleic acid, and protein levels. Cell migration was visualized by phase-contrast microscopy, employing conventional culture inserts to make cell-free areas. Together, 93 polyphenolic and monoterpenoid compounds were characterized, including flavonoid glycosides, lignans, hydroxycinnamic acid, and hydroxybenzoic acid derivatives, as well as iridoids and secoiridoids. The ethanolic extract showed high antioxidant capacity and strong antimicrobial activity against Bacillus subtilis (MIC80 value: 354.37 ± 4.58 µg/mL) and Streptococcus pyogenes (MIC80 value: 488.89 ± 4.75 µg/mL). The abundance of phenolic compounds and the results of biological tests indicate the potential for L. tuberosus to serve as reservoirs of bioactive compounds and to be used in the development of novel nutraceuticals.},
	year = {2024},
	eissn = {2223-7747},
	orcid-numbers = {Alberti, Ágnes/0000-0003-3096-4057; Felegyi-Tóth, Csenge Anna/0000-0001-6260-465X; Czigle, Szilvia/0000-0002-4634-3311}
}

@article{MTMT:34486045,
	title = {Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker},
	url = {https://m2.mtmt.hu/api/publication/34486045},
	author = {Kurdi, Csilla and Schmidt, János and Horváth-Szalai, Zoltán and Mauchart, Péter and Gödöny, Krisztina and Várnagy, Ákos and Kovács, L. Gábor and Kőszegi, Tamás},
	doi = {10.3390/ijms25010486},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34486045},
	issn = {1661-6596},
	abstract = {Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility, and this fluid is a critical microenvironment for the developing oocytes as well. Using the high-performance liquid chromatography-mass spectrometry method, we investigated the protein composition of the follicular fluid, and after classification, we carried out relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein–protein interaction analysis, albumin and apolipoprotein A1 (ApoA1) were found to be hub proteins, and the quantitative comparison of the P and NP groups resulted in a significantly lower concentration of ApoA1 and high-density lipoprotein cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility.},
	keywords = {PREDICTIVE VALUE; Mass spectrometry; follicular fluid; proteomics; apolipoprotein A1; HDL CHOLESTEROL; assisted reproductive treatment},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Kovács, L. Gábor/0000-0001-5298-5401}
}

@article{MTMT:34449761,
	title = {THE DIAGNOSTIC AND PROGNOSTIC ROLE OF URINARY PROTEIN MARKERS IN PATIENTS WITH SARS-COV-2 INFECTION},
	url = {https://m2.mtmt.hu/api/publication/34449761},
	author = {Szirmay, Balázs and Horváth-Szalai, Zoltán and Ludány, Andrea and Miseta, Attila János and Wittmann, István and Kőszegi, Tamás},
	journal-iso = {CLIN CHEM LAB MED},
	journal = {CLINICAL CHEMISTRY AND LABORATORY MEDICINE},
	volume = {61},
	unique-id = {34449761},
	issn = {1434-6621},
	year = {2023},
	eissn = {1437-4331},
	pages = {s738-s738},
	orcid-numbers = {Miseta, Attila János/0000-0002-7984-3347}
}

@article{MTMT:34227655,
	title = {The Effect of Holder Pasteurization and Different Variants on Breast Milk Antioxidants},
	url = {https://m2.mtmt.hu/api/publication/34227655},
	author = {Vass, Réka Anna and Mikó, Éva and Gál, Csenge and Kőszegi, Tamás and Vass, Csaba I and Bokor, Szilvia and Molnár, Dénes and Funke Kiss, Simone and Kovács, Kálmán András and Bódis, József and Ertl, Tibor},
	doi = {10.3390/antiox12101857},
	journal-iso = {ANTIOXIDANTS-BASEL},
	journal = {ANTIOXIDANTS},
	volume = {12},
	unique-id = {34227655},
	abstract = {After birth, breast milk (BM) is a known essential source of antioxidants for infants. We analyzed the non-enzymatic total antioxidant capacity (TAC), oxygen radical absorbance capacity (ORAC), and glutathione, calcium, transferrin, and total protein levels of human breast milk before and after Holder pasteurization (HoP).The collected donor BM samples were pasteurized with HoP.HoP decreased TAC (-12.6%), ORAC (-12.1%), transferrin (-98.3%), and total protein (-21.4%) levels; HoP did not influence the glutathione concentration, and it increased the total calcium (+25.5%) concentration. Mothers who gave birth via Cesarean section had significantly lower TAC in their BM. TAC and glutathione levels were elevated in the BM of mothers over the age of 30. BM produced in the summer had higher glutathione and calcium levels compared to BM produced in the winter. The glutathione concentration in term milk samples was significantly higher in the first two months of lactation compared to the period between the third and sixth months. The transferrin level of BM for female infants was significantly higher than the BM for boys, and mothers with a BMI above 30 had increased transferrin in their samples.Antioxidant levels in human milk are influenced by numerous factors. Environmental and maternal factors, the postpartum age at breast milk collection, and Holder pasteurization of the milk influence the antioxidant intake of the infant.},
	keywords = {calcium; GLUTATHIONE; TRANSFERRIN; TAC; breast milk; Total protein; donor milk; ORAC},
	year = {2023},
	eissn = {2076-3921},
	orcid-numbers = {Vass, Réka Anna/0000-0002-0436-4261; Molnár, Dénes/0000-0002-3675-7019}
}

@article{MTMT:34167218,
	title = {Measurement of Urinary Gc-Globulin by a Fluorescence ELISA Technique: Method Validation and Clinical Evaluation in Septic Patients—A Pilot Study},
	url = {https://m2.mtmt.hu/api/publication/34167218},
	author = {Kőszegi, Tamás and Horváth-Szalai, Zoltán and Ragán, Dániel and Kósa, Brigitta and Szirmay, Balázs and Kurdi, Csilla and Kovács, L. Gábor and Mühl, Diána},
	doi = {10.3390/molecules28196864},
	journal-iso = {MOLECULES},
	journal = {MOLECULES},
	volume = {28},
	unique-id = {34167218},
	issn = {1420-3049},
	abstract = {A major complication of sepsis is the development of acute kidney injury (AKI). In case of acute tubular damage, Gc-globulin, a known serum sepsis marker is increasingly filtrated into the urine therefore, urinary Gc-globulin (u-Gc) levels may predict septic AKI. We developed and validated a competitive fluorescence ELISA method for u-Gc measurement. Serum and urine samples from septic patients were collected in three consecutive days (T1, T2, T3) and data were compared to controls. Intra- and interassay imprecisions were CV < 14% and CV < 20%, respectively, with a recovery close to 100%. Controls and septic patients differed (p < 0.001) in their u-Gc/u-creatinine levels at admission (T1, median: 0.51 vs. 79.1 µg/mmol), T2 (median: 0.51 vs. 57.8 µg/mmol) and T3 (median: 0.51 vs. 55.6 µg/mmol). Septic patients with AKI expressed higher u-Gc/u-creatinine values than those without AKI at T1 (median: 23.6 vs. 136.5 µg/mmol, p < 0.01) and T3 (median: 34.4 vs. 75.8 µg/mmol, p < 0.05). AKI-2 stage patients exhibited more increased u-Gc/u-creatinine levels at T1 (median: 207.1 vs. 53.3 µg/mmol, p < 0.05) than AKI-1 stage individuals. Moderate correlations (p < 0.001) were observed between u-Gc/u-creatinine and se-urea, se-creatinine, se-hsCRP, WBC, u-total protein, u-albumin, u-orosomucoid/u-creatinine, and u-Cystatin C/u-creatinine levels. U-Gc testing may have a predictive value for AKI in septic patients.},
	year = {2023},
	eissn = {1420-3049},
	orcid-numbers = {Kovács, L. Gábor/0000-0001-5298-5401}
}

@article{MTMT:34113082,
	title = {Serum Total Antioxidant Capacity (TAC) and TAC/Lymphocyte Ratio as Promising Predictive Markers in COVID-19},
	url = {https://m2.mtmt.hu/api/publication/34113082},
	author = {Horváth-Szalai, Zoltán and Jakabfi-Csepregi, Rita and Szirmay, Balázs and Ragán, Dániel and Simon, Gerda and Kovács-Ábrahám, Zoltán and Szabó, Péter and Sipos, Dávid and Péterfalvi, Ágnes and Miseta, Attila János and Csontos, Csaba and Kőszegi, Tamás and Némethné Tóth, Ildikó},
	doi = {10.3390/ijms241612935},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34113082},
	issn = {1661-6596},
	abstract = {SARS-CoV-2 infection might cause a critical disease, and patients' follow-up is based on multiple parameters. Oxidative stress is one of the key factors in the pathogenesis of COVID-19 suggesting that its level could be a prognostic marker. Therefore, we elucidated the predictive value of the serum non-enzymatic total antioxidant capacity (TAC) and that of the newly introduced TAC/lymphocyte ratio in COVID-19. We included 61 COVID-19 (n = 27 ward, n = 34 intensive care unit, ICU) patients and 29 controls in our study. Serum TAC on admission was measured by an enhanced chemiluminescence (ECL) microplate assay previously validated by our research group. TAC levels were higher (p < 0.01) in ICU (median: 407.88 µmol/L) than in ward patients (315.44 µmol/L) and controls (296.60 µmol/L). Besides the classical parameters, both the TAC/lymphocyte ratio and TAC had significant predictive values regarding the severity (AUC-ROC for the TAC/lymphocyte ratio: 0.811; for TAC: 0.728) and acute kidney injury (AUC-ROC for the TAC/lymphocyte ratio: 0.747; for TAC: 0.733) in COVID-19. Moreover, the TAC/lymphocyte ratio had significant predictive value regarding mortality (AUC-ROC: 0.752). Serum TAC and the TAC/lymphocyte ratio might offer valuable information regarding the severity of COVID-19. TAC measured by our ECL microplate assay serves as a promising marker for the prediction of systemic inflammatory diseases.},
	keywords = {PREDICTIVE VALUE; enhanced chemiluminescence; COVID-19; TAC/lymphocyte ratio; serum total antioxidant capacity (TAC)},
	year = {2023},
	eissn = {1422-0067},
	orcid-numbers = {Kovács-Ábrahám, Zoltán/0009-0005-2516-8349; Miseta, Attila János/0000-0002-7984-3347}
}

@article{MTMT:34106678,
	title = {Amino Acid Profiling of Follicular Fluid in Assisted Reproduction Reveals Important Roles of Several Amino Acids in Patients with Insulin Resistance},
	url = {https://m2.mtmt.hu/api/publication/34106678},
	author = {Kurdi, Csilla and Lelovics, Vanessza and Hesszenberger, Dávid and Lajtai, Anikó and Lakatos, Ágnes and Herczeg, Róbert and Gödöny, Krisztina and Mauchart, Péter and Várnagy, Ákos and Kovács, L. Gábor and Kőszegi, Tamás},
	doi = {10.3390/ijms241512458},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34106678},
	issn = {1661-6596},
	abstract = {The global prevalence of insulin resistance (IR) is increasing continuously, influencing metabolic parameters and fertility. The metabolic changes due to IR can alter the molecular composition of plasma and other body fluids. Follicular fluid (FF) is derived mainly from plasma, and it is a critical microenvironment for the developing oocytes. It contains various metabolites and amino acids, and the quality of the oocytes is linked at least partially to amino acid metabolism. Our goal was to quantitatively determine the amino acid (AA) profile of FF in IVF patients and to compare IR and non-insulin resistance (NIR) groups to investigate the AA changes in their FF. Using UHPLC-based methods, we quantified the main 20 amino acids from human FF samples in the IR and NIR groups. Several amino acids (aspartate, glycine, glutamate, and cysteine) differed significantly (p < 0.05 or less) between the two groups. The most significant alterations between the IR and NIR groups were related to the glutathione metabolic pathway involving glycine, serine, and threonine. Since insulin resistance alters the amino acid composition of the FF, the oocytes may undergo metabolism-induced changes resulting in poor oocyte quality and less fertility in the insulin resistance groups.},
	keywords = {PREDICTIVE VALUE; Insulin Resistance; follicular fluid; metabolomics; Amino acid profile; Assisted reproduction technology},
	year = {2023},
	eissn = {1422-0067},
	orcid-numbers = {Herczeg, Róbert/0000-0002-5903-0082; Kovács, L. Gábor/0000-0001-5298-5401}
}

@article{MTMT:34081443,
	title = {Apelin-13 as a Potential Biomarker in Critical Illness},
	url = {https://m2.mtmt.hu/api/publication/34081443},
	author = {Gergics, Marin and Pham-Dobor, Gréta and Kurdi, Csilla and Montskó, Gergely and Mihályi, Krisztina and Bánfai, Gábor László and Kanizsai, Péter László and Kőszegi, Tamás and Mezősi, Emese and Bajnok, László Zoltán},
	doi = {10.3390/jcm12144801},
	journal-iso = {J CLIN MED},
	journal = {JOURNAL OF CLINICAL MEDICINE},
	volume = {12},
	unique-id = {34081443},
	abstract = {The adrenocortical system and copeptin as prognostic markers were intensively investigated in critical illness. The potential predictive power of apelin-13 as a biomarker is largely unknown. We aimed to investigate the prognostic role of apelin-13 in relation to free cortisol, aldosterone, CRH, and copeptin in critically ill patients.In this prospective observational study, 124 critically ill patients (64 men, 60 women, median age: 70 (59-78) years) were consecutively enrolled at the time of admission. All routinely available clinical and laboratory parameters were evaluated and correlated to hormonal changes.Serum apelin-13 was 1161 (617-2967) pg/mL in non-survivors vs. 2477 (800-3531) pg/mL in survivors (p = 0.054). The concentrations of apelin-13 and CRH had strong positive correlations (r = 0.685, p < 0.001) and were significantly higher in surviving non-septic patients (Apelin-13 (pg/mL): 2286 (790-3330) vs. 818 (574-2732) p < 0.05; CRH (pg/mL) 201 (84-317) vs. 89 (74-233) p < 0.05). Apelin-13 and free cortisol were independent determinants of survival in the multivariate Cox regression analysis, while copeptin, CRH, or aldosterone were not.Beyond free cortisol, serum apelin-13 may also help refine prognostic predictions in the early phase of critical illness, especially in non-septic patients.},
	keywords = {critical illness; CRH; Apelin-13; COPEPTIN; Free cortisol},
	year = {2023},
	eissn = {2077-0383},
	orcid-numbers = {Kanizsai, Péter László/0000-0001-7896-2857; Mezősi, Emese/0000-0001-9367-3877}
}

@article{MTMT:34036926,
	title = {The Role of Two Heart Biomarkers in IgA Nephropathy},
	url = {https://m2.mtmt.hu/api/publication/34036926},
	author = {Sági, Balázs and Vas, Tibor and Jakabfi-Csepregi, Rita and Horváth-Szalai, Zoltán and Kőszegi, Tamás and Csiky, Botond and Nagy, Judit and Kovács, Tibor},
	doi = {10.3390/ijms241210336},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {24},
	unique-id = {34036926},
	issn = {1661-6596},
	abstract = {Cardiovascular mortality is a leading cause of death in chronic kidney disease (CKD), as is IgA nephropathy (IgAN). The purpose of this study is to find different biomarkers to estimate the outcome of the disease, which is significantly influenced by the changes in vessels (characterized by arterial stiffness) and the heart. In our cross-sectional study, 90 patients with IgAN were examined. The N-terminal prohormone of brain natriuretic peptide (NT-proBNP) was measured as a heart failure biomarker by an automated immonoassay method, while the carboxy-terminal telopeptide of collagen type I (CITP) as a fibrosis marker was determined using ELISA kits. Arterial stiffness was determined by measuring carotid–femoral pulse wave velocity (cfPWV). Renal function and routine echocardiography examinations were performed as well. Based on eGFR, patients were separated into two categories, CKD 1-2 and CKD 3-5. There were significantly higher NT-proBNP (p = 0.035), cfPWV (p = 0.004), and central aortic systolic pressure (p = 0.037), but not CITP, in the CKD 3-5 group. Both biomarker positivities were significantly higher in the CKD 3-5 group (p = 0.035) compared to the CKD 1-2 group. The central aortic systolic pressure was significantly higher in the diastolic dysfunction group (p = 0.034), while the systolic blood pressure was not. eGFR and hemoglobin levels showed a strong negative correlation, while left ventricular mass index (LVMI), aortic pulse pressure, central aortic systolic pressure, and cfPWV showed a positive correlation with NT-proBNP. cfPWV, aortic pulse pressure, and LVMI showed a strong positive correlation with CITP. Only eGFR was an independent predictor of NT-proBNP by linear regression analysis. NT-proBNP and CITP biomarkers may help to identify IgAN patients at high risk for subclinical heart failure and further atherosclerotic disease.},
	year = {2023},
	eissn = {1422-0067}
}

@article{MTMT:33811185,
	title = {Serum albumin binding studies on antiproliferative cyclic C5-curcuminoid derivatives using spectroscopic methods and molecular modelling},
	url = {https://m2.mtmt.hu/api/publication/33811185},
	author = {Tyukodi, Levente and Zsidó, Balázs Zoltán and Hetényi, Csaba and Kőszegi, Tamás and Huber, Imre and Rozmer, Zsuzsanna},
	doi = {10.1016/j.molstruc.2023.135761},
	journal-iso = {J MOL STRUCT},
	journal = {JOURNAL OF MOLECULAR STRUCTURE},
	volume = {1287},
	unique-id = {33811185},
	issn = {0022-2860},
	year = {2023},
	eissn = {1872-8014},
	orcid-numbers = {Tyukodi, Levente/0000-0002-8983-1876; Huber, Imre/0000-0003-0217-0188}
}