TY - JOUR AU - Datki, Zsolt László AU - Darula, Zsuzsanna AU - Vedelek, Viktor AU - Hunyadi-Gulyás Éva, Csilla AU - Dingmann, Brian J. AU - Vedelek, Balázs AU - Kalman, Janos AU - Urban, Peter AU - Gyenesei, Attila AU - Galik-Olah, Zita AU - Gálik, Bence AU - Sinka, Rita TI - Biofilm formation initiating rotifer-specific biopolymer and its predicted components JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 253 PY - 2023 IS - Part 5 PG - 14 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2023.127157 UR - https://m2.mtmt.hu/api/publication/34334193 ID - 34334193 N1 - Megosztott szerzőség AB - The rotifer-specific biopolymer, namely Rotimer, is a recently discovered group of the biomolecule family. Rotimer has an active role in the biofilm formation initiated by rotifers (e.g., Euchlanis dilatata or Adineta vaga) or in the female-male sexual interaction of monogononts. To understand the Ca2+- and polarity-dependent formation of this multifunctional viscoelastic material, it is essential to explore its molecular composition. The investigation of the rotifer-enhanced biofilm and Rotimer-inductor conglomerate (RIC) formation yielded several protein candidates to predict the Rotimer-specific main components. The exudate of E. dilatata males was primarily applied from different biopolimer-containing samples (biofilm or RIC). The advantage of males over females lies in their degenerated digestive system and simple anatomy. Thus, their exudate is less contaminated with food and endosymbiont elements. The sequenced and annotated genome and transcriptome of this species opened the way for identifying Rotimer proteins by mass spectrometry. The predicted rotifer-biopolymer forming components are SCO-spondins and 14-3-3 protein. The characteristics of Rotimer are similar to Reissner's fiber, which is found in the central nervous system of vertebrates and is mainly formed from SCO-spondins. This molecular information serves as a starting point for its interdisciplinary investigation and application in biotechnology, biomedicine, or neurodegeneration-related drug development. LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Sinka, Rita TI - Translational biomedicine-oriented exploratory research on bioactive rotifer-specific biopolymers JF - ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE J2 - ADV CLIN EXP MED VL - 31 PY - 2022 IS - 9 SP - 931 EP - 935 PG - 5 SN - 1899-5276 DO - 10.17219/acem/152430 UR - https://m2.mtmt.hu/api/publication/33084796 ID - 33084796 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Sinka, Rita AU - Gálik, Bence AU - Galik-Olah, Zita TI - Particle-dependent reproduction and exogenic biopolymer secretion of protozoa co-cultured rotifers JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 211 PY - 2022 SP - 669 EP - 677 PG - 9 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2022.05.020 UR - https://m2.mtmt.hu/api/publication/32830008 ID - 32830008 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Balázs, Evelin AU - Gálik, Bence AU - Sinka, Rita AU - Zeitler, Lavínia AU - Bozsó, Zsolt AU - Kálmán, János AU - Hortobágyi, Tibor AU - Oláh, Zita TI - The interacting rotifer-biopolymers are anti- and disaggregating agents for human-type beta-amyloid in vitro JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 201 PY - 2022 SP - 262 EP - 269 PG - 8 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2021.12.184 UR - https://m2.mtmt.hu/api/publication/32591465 ID - 32591465 N1 - Funding Agency and Grant Number: Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Found [UNKP-21-5-SZTE-555]; European Union [754432]; Polish Ministry of Science and Higher Education; SZTEAOK-KKA [5S 567 (A202)]; Developing Scientific Workshops of Medical-, Health Sciences and Pharmaceutical Training (Hungary) [EFOP 3.6.3-VEKOP-16-201700009] Funding text: This project was supported by the J ' anos Bolyai Research Scholarship of the Hungarian Academy of Sciences; by the UNKP-21-5-SZTE-555 New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Found; by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement, Nr. 754432; by the Polish Ministry of Science and Higher Education; by the SZTE ' AOK-KKA No. 5S 567 (A202) and by the Developing Scientific Workshops of Medical-, Health Sciences and Pharmaceutical Training (grant number: EFOP 3.6.3-VEKOP-16-201700009; Hungary). LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Erika Hajnalka AU - Kókai, Andrea AU - Kovács, Ildikó AU - Datki, Zsolt László AU - Andó, Bálint AU - Terenyi, Zoltán TI - Pszichoterápiás attitűdök áttekintése és követésének lehetőségei JF - PSYCHIATRIA HUNGARICA J2 - PSYCHIATRIA HUNG VL - 34 PY - 2021 IS - Suppl 1 SP - 3 EP - 3 PG - 1 SN - 0237-7896 UR - https://m2.mtmt.hu/api/publication/32129885 ID - 32129885 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Tóth, Erika Hajnalka AU - Kovács, Ildikó AU - Datki, Zsolt László AU - Andó, Bálint TI - Rogersi hatótényezők biológiai-pszichofiziológiai paraméterekkel történő vizsgálatainak lehetőségei JF - PSYCHIATRIA HUNGARICA J2 - PSYCHIATRIA HUNG VL - 36 PY - 2021 IS - Suppl 1 SP - 2 EP - 2 PG - 1 SN - 0237-7896 UR - https://m2.mtmt.hu/api/publication/32129874 ID - 32129874 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Balázs, Evelin AU - Oláh, Zita AU - Gálik, Bence AU - Somogyvári, Ferenc AU - Kálmán, János AU - Datki, Zsolt László TI - External modulation of Rotimer exudate secretion in monogonant rotifers JF - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY J2 - ECOTOX ENVIRON SAFE VL - 220 PY - 2021 PG - 8 SN - 0147-6513 DO - 10.1016/j.ecoenv.2021.112399 UR - https://m2.mtmt.hu/api/publication/32059771 ID - 32059771 AB - The Rotimer, a rotifer-specific biopolymer, is an exogenic bioactive exudate secreted by different monogonant species (e.g. Euchlanis dilatata or Lecane bulla). The production of this viscoelastic biomolecule is induced by different micro-particles, thereby forming a special Rotimer-Inductor Conglomerate (RIC) in a web format. In this case, the water insoluble Carmine crystals, filtered to size (max. diameter was 50 µm), functioned as an inductor. The RIC production is an adequate empirical indicator to follow up this filamentous biopolymer secretion experientially; moreover, this procedure is very sensitive to the environmental factors (temperature, pH, metals and possible natural pollutant agents). The above mentioned species show completely different reactions to these factors, except to the presence of calcium and to the modulating effects of different drugs. One of the novelties of this work is that the Rotimer secretion and consequently, the RIC-formation is a mutually obligatory and evolutionary calcium-dependent process in the concerned monogonants. This in vivo procedure needs calcium, both for the physiology of animals and for fiber formation, particularly in the latter case. The conglomerate covered area (%) and the detection of the longest filament (mm) of the given RIC were the generally and simultaneously applied methods in the current modulating experiments. Exploring the regulatory (e.g. calcium-dependency) and stimulating (e.g. Lucidril effect) possibilities of biopolymer secretion are the basis for optimizing the RIC-production capacities of these micro-metazoans. LA - English DB - MTMT ER - TY - JOUR AU - Pribék, Ildikó Katalin AU - Szűcs, Kálmán Ferenc AU - Süle, Miklós AU - Grosz, György AU - Ducza, Eszter AU - Vigh, Dóra AU - Tóth, Erika Hajnalka AU - Janka, Zoltán AU - Kálmán, János AU - Datki, Zsolt László AU - Gáspár, Róbert AU - Andó, Bálint TI - Detection of acute stress by smooth muscle electromyography: A translational study on rat and human JF - LIFE SCIENCES J2 - LIFE SCI VL - 277 PY - 2021 PG - 9 SN - 0024-3205 DO - 10.1016/j.lfs.2021.119492 UR - https://m2.mtmt.hu/api/publication/31971856 ID - 31971856 N1 - Department of Psychiatry, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary MDE GmbH, Walldorf, Germany Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary Export Date: 1 June 2021 CODEN: LIFSA Correspondence Address: Gáspár, R.12 Dóm tér, Hungary; email: gaspar.robert@med.u-szeged.hu Correspondence Address: Andó, B.8-10 Korányi fasor, Hungary; email: ando.balint@med.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Ács, Éva AU - Balázs, Evelin AU - Sovány, Tamás AU - Pannonhalminé Csóka, Ildikó AU - Zsuga, Katalin AU - Kálmán, János AU - Oláh, Zita TI - Exogenic production of bioactive filamentous biopolymer by monogonant rotifers JF - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY J2 - ECOTOX ENVIRON SAFE VL - 208 PY - 2021 PG - 10 SN - 0147-6513 DO - 10.1016/j.ecoenv.2020.111666 UR - https://m2.mtmt.hu/api/publication/31776926 ID - 31776926 N1 - A publikáció a Nemzeti Közszolgálati Egyetem 2020. évi Tématerületi Kiválóság Program keretében, a Fenntartható biztonság és társadalmi környezet elnevezésű projekt támogatásával valósult meg, az Innovációs és Technológiai Minisztérium Nemzeti Kutatási, Fejlesztési és Innovációs Alapból nyújtott támogatásával, a Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal által kibocsátott támogatói okirat alapján. AB - The chemical ecology of rotifers has been little studied. A yet unknown property is presented within some monogonant rotifers, namely the ability to produce an exogenic filamentous biopolymer, named ‘Rotimer’. This rotifer-specific viscoelastic fiber was observed in six different freshwater monogonants (Euchlanis dilatata, Lecane bulla, Lepadella patella, Itura aurita, Colurella adriatica and Trichocerca iernis) in exception of four species. Induction of Rotimer secretion can only be achieved by mechanically irritating rotifer ciliate with administering different types (yeast cell skeleton, denatured BSA, epoxy, Carmine or urea crystals and micro-cellulose) and sizes (approx. from 2.5 to 50 μm diameter) of inert particles, as inductors or visualization by adhering particles. The thickness of this Rotimer is 33 ± 3 nm, detected by scanning electron microscope. This material has two structural formations (fiber or gluelike) in nano dimension. The existence of the novel adherent natural product becomes visible by forming a ‘Rotimer-Inductor Conglomerate’ (RIC) web structure within a few minutes. The RIC-producing capacity of animals, depends on viability, is significantly modified according to physiological-(depletion), drug- (toxin or stimulator) and environmental (temperature, salt content and pH) effects. The E. dilatata-produced RIC is affected by protein disruptors but is resistant to several chemical influences and its Rotimer component has an overwhelming cell (algae, yeast and human neuroblastoma) motility inhibitory effect, associated with low toxicity. This biopolymer-secretion-capacity is protective of rotifers against human-type beta-amyloid aggregates. LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Evelin AU - Oláh, Zita AU - Gálik, Bence AU - Bozsó, Zsolt AU - Kálmán, János AU - Datki, Zsolt László TI - Neurodegeneration-related beta-amyloid as autocatabolism-attenuator in a micro-in vivo system JF - IBRO REPORTS J2 - IBRO REP VL - 9 PY - 2020 SP - 319 EP - 323 PG - 5 SN - 2451-8301 DO - 10.1016/j.ibror.2020.10.002 UR - https://m2.mtmt.hu/api/publication/31639846 ID - 31639846 LA - English DB - MTMT ER -