TY - JOUR AU - Makkai, Géza AU - Ábrahám, István AU - Barabás, Klaudia AU - Godó, Soma AU - Ernszt, Dávid AU - Kovács, Tamás AU - Kovács, Gergely AU - Szőcs, Szilárd AU - Jánosi, Tibor Zoltán TI - Maximum likelihood-based estimation of diffusion coefficient is quick and reliable method for analyzing estradiol actions on surface receptor movements JF - FRONTIERS IN NEUROINFORMATICS J2 - FRONT NEUROINFORM VL - 17 PY - 2023 PG - 10 SN - 1662-5196 DO - 10.3389/fninf.2023.1005936 UR - https://m2.mtmt.hu/api/publication/33704619 ID - 33704619 N1 - Institute of Physiology, Medical School, University of Pécs, Pécs, Hungary Nano-Bio-Imaging Core Facility at the Szentágothai Research Centre of the University of Pécs, Pécs, Hungary Centre for Neuroscience, Szentágothai Research Centre, University of Pécs, Pécs, Hungary Export Date: 14 November 2023 Correspondence Address: Janosi, T.Z.; Institute of Physiology, Hungary; email: tzjanosi@gamma.ttk.pte.hu Chemicals/CAS: estradiol, 50-28-2 Tradenames: Matlab Manufacturers: Olympus, Japan Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA, TKP-2021-EGA-16 Funding text 1: TKP2021-EGA-16 has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-EGA funding scheme. DE was supported by the únkp-21-4-II new national excellence program of the ministry for innovation and technology from the source of the National Research, Development and Innovation Fund. SS acknowledges the support of National Research, Development and Innovation Fund of Hungary (TKP-2021-EGA-16). AB - The rapid effects of estradiol on membrane receptors are in the focus of the estradiol research field, however, the molecular mechanisms of these non-classical estradiol actions are poorly understood. Since the lateral diffusion of membrane receptors is an important indicator of their function, a deeper understanding of the underlying mechanisms of non-classical estradiol actions can be achieved by investigating receptor dynamics. Diffusion coefficient is a crucial and widely used parameter to characterize the movement of receptors in the cell membrane. The aim of this study was to investigate the differences between maximum likelihood-based estimation (MLE) and mean square displacement (MSD) based calculation of diffusion coefficients. In this work we applied both MSD and MLE to calculate diffusion coefficients. Single particle trajectories were extracted from simulation as well as from α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor tracking in live estradiol-treated differentiated PC12 (dPC12) cells. The comparison of the obtained diffusion coefficients revealed the superiority of MLE over the generally used MSD analysis. Our results suggest the use of the MLE of diffusion coefficients because as it has a better performance, especially for large localization errors or slow receptor movements. LA - English DB - MTMT ER - TY - JOUR AU - Godó, Soma AU - Barabás, Klaudia AU - Lengyel, Ferenc AU - Ernszt, Dávid AU - Kovács, Tamás AU - Kecskés, Miklós AU - Varga, Csaba AU - Jánosi, Tibor Zoltán AU - Makkai, Géza AU - Kovács, Gergely AU - Orsolits, Barbara AU - Fujiwara, Takahiro AU - Kusumi, Akihiro AU - Ábrahám, István TI - Single-Molecule Imaging Reveals Rapid Estradiol Action on the Surface Movement of AMPA Receptors in Live Neurons JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 9 PY - 2021 PG - 17 SN - 2296-634X DO - 10.3389/fcell.2021.708715 UR - https://m2.mtmt.hu/api/publication/32235080 ID - 32235080 N1 - PTE-NAP Molecular Neuroendocrinology Research Group, Centre for Neuroscience, Szentágothai Research Center, Medical School, Institute of Physiology, University of Pécs, Pécs, Hungary PTE-NAP Cortical Microcircuits Research Group, Institute of Physiology, Medical School, Centre for Neuroscience, Szentágothai Research Institute, Pécs, Hungary Laboratory of Neuroimmunology, Institute of Experimental Medicine of the Hungarian Academy of Sciences, Budapest, Hungary Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto, Japan Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST)Onna, Japan Cited By :2 Export Date: 6 November 2023 Correspondence Address: Godó, S.; PTE-NAP Molecular Neuroendocrinology Research Group, Hungary; email: soma.godo@aok.pte.hu Chemicals/CAS: estradiol, 50-28-2 Funding details: KTIA_NAP_13-2014-0001,20017-1.2.1-NKP-2017-00002 Funding details: European Commission, EC Funding details: Hungarian Scientific Research Fund, OTKA, 112807 Funding details: European Social Fund, ESF, EFOP-3.6.1 Funding details: Emberi Eroforrások Minisztériuma, EMMI Funding details: Pécsi Tudományegyetem, PTE, EFOP 3.6.2-16-2017-00008 Funding details: Innovációs és Technológiai Minisztérium, GINOP-2.3.2-15-2016-00048, GINOP-2.3.3-15-2016-00030, ÚNKP-18-3-III Funding text 1: We thank Allan Herbison for valuable comments on this manuscript and Imre Farkas for the generous gift of DPN. The research was performed in collaboration with the Nano-Bio-Imaging core facility at the Szentágothai Research Centre of the University of Pécs. We also wish to thank to the Nikon Microscopy Center at the Institute of Experimental Medicine (IEM), Nikon Austria GmbH as well as Auro-Science Consulting Ltd for kindly providing microscopy support. Funding text 2: This work was supported by the Hungarian Brain Research Program (KTIA_NAP_13-2014-0001,20017-1.2.1-NKP-2017-00002), Hungarian Scientific Research Fund (OTKA; 112807), and European Union, and was co-financed by the European Social Fund under the following grants: EFOP-3.6.1.-16-2016-00004 (Comprehensive Development for Implementing Smart Specialization Strategies at the University of Pécs), EFOP 3.6.2-16-2017-00008 (The Role of Neuroinflammation in Neurodegeneration: From Molecules to Clinics), the Higher Education Institutional Excellence Program of the Ministry for Innovation and Technology in Hungary, within the framework of the (5th thematic program) of the University of Pécs, GINOP-2.3.2-15-2016-00048 (Stay Alive), GINOP-2.3.3-15-2016-00030 (Nano-Bioimaging), and ÚNKP-18-3-III (New National Excellence Program of the Ministry of Human Capacities). AB - Gonadal steroid 17β-estradiol (E2) exerts rapid, non-genomic effects on neurons and strictly regulates learning and memory through altering glutamatergic neurotransmission and synaptic plasticity. However, its non-genomic effects on AMPARs are not well understood. Here, we analyzed the rapid effect of E2 on AMPARs using single-molecule tracking and super-resolution imaging techniques. We found that E2 rapidly decreased the surface movement of AMPAR via membrane G protein-coupled estrogen receptor 1 (GPER1) in neurites in a dose-dependent manner. The cortical actin network played a pivotal role in the GPER1 mediated effects of E2 on the surface mobility of AMPAR. E2 also decreased the surface movement of AMPAR both in synaptic and extrasynaptic regions on neurites and increased the synaptic dwell time of AMPARs. Our results provide evidence for understanding E2 action on neuronal plasticity and glutamatergic neurotransmission at the molecular level. LA - English DB - MTMT ER - TY - JOUR AU - Horváth, Ádám AU - Payrits, Maja AU - Steib, Anita AU - Kántás, Boglárka AU - Biró-Sütő, Tünde AU - Erostyák, János AU - Makkai, Géza AU - Sághy, Éva AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Analgesic Effects of Lipid Raft Disruption by Sphingomyelinase and Myriocin via Transient Receptor Potential Vanilloid 1 and Transient Receptor Potential Ankyrin 1 Ion Channel Modulation JF - FRONTIERS IN PHARMACOLOGY J2 - FRONT PHARMACOL VL - 11 PY - 2021 PG - 11 SN - 1663-9812 DO - 10.3389/fphar.2020.593319 UR - https://m2.mtmt.hu/api/publication/31848850 ID - 31848850 N1 - Funding Agency and Grant Number: National Brain Research Program [2017-1.2.1-NKP -2017-00002]; Hungarian Government [GINOP-2.3.2-15-2016-00050, EFOP-3.6.2-16-2017-00006, EFOP-3.6.2-16-2017-00008]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; University of Pecs [17886-4/23018/FEKUTSTRAT]; New National Excellence Program of the Ministry of Human Capacities [UNKP-18-4]; Gedeon Richter's Talentum Foundation; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-19-4] Funding text: This work was supported by the National Brain Research Program 2017-1.2.1-NKP -2017-00002 (NAP-2; Chronic Pain Research Group). We acknowledge the grant of the Hungarian Government (GINOP-2.3.2-15-2016-00050, EFOP-3.6.2-16-2017-00006 and EFOP-3.6.2-16-2017-00008). ESz and ESa were supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. The University of Pecs is acknowledged for a support by the 17886-4/23018/FEKUTSTRAT excellence grant. MP was supported by the New National Excellence Program of the Ministry of Human Capacities UNKP-18-4. ESz and ESa were supported by the New National Excellence Program of the Ministry of Human Capacities UNKP-18-4 and New National Excellence Program of the Ministry for Innovation and TechnologyUNKP-19-4 grant. AHwas supported by the Gedeon Richter's Talentum Foundation. LA - English DB - MTMT ER - TY - CHAP AU - Buzády, Andrea AU - Gálos, Réka AU - Makkai, Géza AU - Xiaojun, Wu AU - Tóth, György AU - Kovács, László AU - Almási, Gábor AU - Hebling, János AU - Pálfalvi, László ED - Földi, Péter ED - Magashegyi, István TI - Magnéziummal adalékolt sztöchiometrikus lítium- niobát hőméréskletfüggő dielektromos paraméterei a terahertzes tartományon T2 - Kvantumelektronika 2021 PB - Szegedi Tudományegyetem TTIK Fizikai Intézet CY - Szeged SN - 9789633067758 PY - 2020 SP - 25 EP - 30 PG - 6 DO - 10.14232/kvantumelektronika.9.5 UR - https://m2.mtmt.hu/api/publication/31887429 ID - 31887429 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szőke, Éva AU - Horvath, Adam AU - Biró-Sütő, Tünde AU - Sághy, Éva AU - Payrits, Maja AU - Erostyák, János AU - Makkai, Géza AU - Szolcsányi, János AU - Helyes, Zsuzsanna TI - In vitro and in vivo evidence for the role of lipid rafts in Ca2+-gating of the Transient Receptor Potential channels in sensory neurons JF - FASEB JOURNAL J2 - FASEB J VL - 34 PY - 2020 IS - S1 PG - 2 SN - 0892-6638 DO - 10.1096/fasebj.2020.34.s1.05167 UR - https://m2.mtmt.hu/api/publication/31625361 ID - 31625361 LA - English DB - MTMT ER - TY - JOUR AU - Payrits, Maja AU - Horváth, Ádám AU - Biró-Sütő, Tünde AU - Erostyák, János AU - Makkai, Géza AU - Sághy, Éva AU - Pohóczky, Krisztina AU - Kecskés, Angéla AU - Kecskés, Miklós AU - Szolcsányi, János AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Resolvin D1 and D2 inhibit transient receptor potential vanilloid 1 and ankyrin 1 ion channel activation on sensory neurons via lipid raft modification JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 14 PG - 17 SN - 1661-6596 DO - 10.3390/ijms21145019 UR - https://m2.mtmt.hu/api/publication/31387590 ID - 31387590 N1 - Funding Agency and Grant Number: National Brain Research Program [2017-1.2.1-NKP-2017-00002]; Hungarian Government [GINOP-2.3.2-15-2016-00050, EFOP-3.6.2-16-2017-00006, EFOP-3.6.2-16-2017-00008]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; University of Pecs [17886-4/23018/FEKUTSTRAT]; New National Excellence Program of the ministry of Human Capacities [UNKP-18-4]; New National Excellence Program of the ministry for Innovation and Technology [UNKP-19-4] Funding text: This work was supported by the National Brain Research Program 2017-1.2.1-NKP-2017-00002 (NAP-2; Chronic Pain Research Group). We acknowledge the grant of the Hungarian Government (GINOP-2.3.2-15-2016-00050, EFOP-3.6.2-16-2017-00006 and EFOP-3.6.2-16-2017-00008). E. Szoke A. Kecskes and E. Saghy were supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. The University of Pecs is acknowledged for a support by the 17886-4/23018/FEKUTSTRAT excellence grant. M. Payrits was supported by the New National Excellence Program of the ministry of Human Capacities UNKP-18-4. E. Szoke and E. Saghy were supported by the New National Excellence Program of the ministry of Human Capacities UNKP-18-4 and New National Excellence Program of the ministry for Innovation and Technology UNKP-19-4 grant. AB - Transient Receptor Potential Vanilloid 1 and Ankyrin 1 (TRPV1, TRPA1) cation channels are expressed in nociceptive primary sensory neurons and regulate nociceptor and inflammatory functions. Resolvins are endogenous lipid mediators. Resolvin D1 (RvD1) is described as a selective inhibitor of TRPA1-related postoperative and inflammatory pain in mice acting on the G protein-coupled receptor DRV1/GPR32. Resolvin D2 (RvD2) is a very potent TRPV1 and TRPA1 inhibitor in DRG neurons, and decreases inflammatory pain in mice acting on the GPR18 receptor, via TRPV1/TRPA1-independent mechanisms. We provided evidence that resolvins inhibited neuropeptide release from the stimulated sensory nerve terminals by TRPV1 and TRPA1 activators capsaicin (CAPS) and allyl-isothiocyanate (AITC), respectively. We showed that RvD1 and RvD2 in nanomolar concentrations significantly decreased TRPV1 and TRPA1 activation on sensory neurons by fluorescent calcium imaging and inhibited the CAPS-and AITC-evoked45Ca-uptake on TRPV1-and TRPA1-expressing CHO cells. Since CHO cells are unlikely to express resolvin receptors, resolvins are suggested to inhibit channel opening through surrounding lipid raft disruption. Here, we proved the ability of resolvins to alter the membrane polarity related to cholesterol composition by fluorescence spectroscopy. It is concluded that targeting lipid raft integrity can open novel peripheral analgesic opportunities by decreasing the activation of nociceptors. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Buzády, Andrea AU - Gálos, R. AU - Makkai, Géza AU - Wu, X. AU - Tóth, György AU - Kovács, László AU - Almási, Gábor AU - Hebling, János AU - Pálfalvi, László TI - Temperature-dependent terahertz time-domain spectroscopy study of Mg-doped stoichiometric lithium niobate JF - OPTICAL MATERIALS EXPRESS J2 - OPT MATER EXPRESS VL - 10 PY - 2020 IS - 4 SP - 998 EP - 1006 PG - 9 SN - 2159-3930 DO - 10.1364/OME.384997 UR - https://m2.mtmt.hu/api/publication/31278977 ID - 31278977 LA - English DB - MTMT ER - TY - JOUR AU - Chisholm, David R. AU - Tomlinson, Charles W. E. AU - Zhou, Garr-Layy AU - Holden, Claire AU - Affleck, Valerie AU - Lamb, Rebecca AU - Newling, Katherine AU - Ashton, Peter AU - Valentine, Roy AU - Redfern, Christopher AU - Erostyák, János AU - Makkai, Géza AU - Ambler, Carrie A. AU - Whiting, Andrew AU - Pohl, Ehmke TI - Fluorescent Retinoic Acid Analogues as Probes for Biochemical and Intracellular Characterization of Retinoid Signaling Pathways JF - ACS CHEMICAL BIOLOGY J2 - ACS CHEM BIOL VL - 14 PY - 2019 IS - 3 SP - 369 EP - 377 PG - 9 SN - 1554-8929 DO - 10.1021/acschembio.8b00916 UR - https://m2.mtmt.hu/api/publication/30545067 ID - 30545067 LA - English DB - MTMT ER - TY - JOUR AU - Sághy, Éva AU - Payrits, Maja AU - Biro-Suto, T AU - Skodáné Földes, Rita AU - Szánti-Pintér, Eszter AU - Erostyák, János AU - Makkai, Géza AU - Sétáló, György (ifj.) AU - Kollár, László AU - Kőszegi, Tamás AU - Jakabfi-Csepregi, Rita AU - Szolcsányi, János AU - Helyes, Zsuzsanna AU - Szőke, Éva TI - Carboxamido steroids inhibit the opening properties of Transient Receptor Potential ion channels by lipid raft modulation. JF - JOURNAL OF LIPID RESEARCH J2 - J LIPID RES VL - 59 PY - 2018 IS - 10 SP - 1851 EP - 1863 PG - 13 SN - 0022-2275 DO - 10.1194/jlr.M084723 UR - https://m2.mtmt.hu/api/publication/3402940 ID - 3402940 N1 - * Megosztott szerzőség AB - Transient Receptor Potential (TRP) cation channels, like the TRP Vanilloid 1 and TRP Ankyrin 1 (TRPV1 and TRPA1) are expressed on primary sensory neurons. These thermosensor channels play role in pain processing. We provided evidence that lipid raft disruption influenced the TRP channel activation and a carboxamido-steroid compound (C1) inhibited TRPV1 activation. Therefore, our aim was to investigate whether this compound exerts its effect through lipid raft disruption and the steroid backbone (C3) or altered position of the carboxamido group (C2) influence the inhibitory action by measuring Ca2+-transients on isolated neurons and calcium-uptake on receptor-expressing CHO cells. Membrane cholesterol content was measured by filipin staining and membrane polarisation by fluorescence spectroscopy. Both the percentage of responsive cells and the magnitude of the intracellular Ca2+-enhancement evoked by the TRPV1 agonist capsaicin were significantly inhibited after C1 and C2 incubation, but not after C3 administration. C1 was able to reduce other TRP channel activation as well. The compounds induced cholesterol depletion in CHO cells, but only C1 induced changes in membrane polarisation. The inhibitory action of the compounds on TRP channel activation develops by lipid raft disruption, and the presence and the position of the carboxamido group is essential. LA - English DB - MTMT ER - TY - JOUR AU - Jánosi, Tibor Zoltán AU - Makkai, Géza AU - Kégl, Tímea AU - Mátyus, Péter AU - Kollár, László AU - Erostyák, János TI - Light-Enhanced Fluorescence of Multi-Level Cavitands Possessing Pyridazine Upper rim JF - JOURNAL OF FLUORESCENCE J2 - J FLUORESC VL - 26 PY - 2016 IS - 2 SP - 679 EP - 688 PG - 10 SN - 1053-0509 DO - 10.1007/s10895-015-1754-3 UR - https://m2.mtmt.hu/api/publication/3000846 ID - 3000846 LA - English DB - MTMT ER -