TY - JOUR AU - Pukleš, Iva AU - Páger, Csilla AU - Sakač, N. AU - Matasović, B. AU - Kovač-Andrić, E. AU - Šarkanj, B. AU - Samardžić, M. AU - Budetić, M. AU - Molnárová, K. AU - Marković, D. AU - Vesinger, A. AU - Jozanović, Marija TI - A new green approach to l-histidine and β-alanine analysis in dietary supplements using rapid and simple contactless conductivity detection integrated with high-resolution glass-microchip electrophoresis JF - ANALYTICAL AND BIOANALYTICAL CHEMISTRY J2 - ANAL BIOANAL CHEM PY - 2024 SN - 1618-2642 DO - 10.1007/s00216-024-05314-9 UR - https://m2.mtmt.hu/api/publication/34864593 ID - 34864593 N1 - Funding Agency and Grant Number: European Structural and Investment Funds Funding text: No Statement Available LA - English DB - MTMT ER - TY - CONF AU - Balogh-Hartmann, Fruzsina AU - Páger, Csilla AU - Bufa, Anita AU - Madarászné Horváth, Ibolya AU - Marosvölgyi, Tamás AU - Makszin, Lilla ED - Kovács, Bettina ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám TI - Adalékanyagtartalmú növényi eredetű tejhelyettesítő italok fehérjetartalmának profilozása mikrofluidikai eljárással T2 - Absztraktkötet: XII. Interdiszciplináris Doktorandusz Konferencia = Book of Abstract: XII. Interdisciplinary Doctoral Conference PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat C1 - Pécs SN - 9789636262457 PY - 2024 SP - 117 EP - 117 PG - 1 UR - https://m2.mtmt.hu/api/publication/34846032 ID - 34846032 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Pukleš, Iva AU - Páger, Csilla AU - Sakač, Nikola AU - Šarkanj, Bojan AU - Matasović, Brunislav AU - Samardžić, Mirela AU - Budetić, Mateja AU - Marković, Dean AU - Jozanović, Marija TI - Electrophoretic Determination of L-Carnosine in Health Supplements Using an Integrated Lab-on-a-Chip Platform with Contactless Conductivity Detection JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 19 PG - 18 SN - 1661-6596 DO - 10.3390/ijms241914705 UR - https://m2.mtmt.hu/api/publication/34188002 ID - 34188002 N1 - ISSN:1422-0067 AB - The health supplement industry is one of the fastest growing industries in the world, but there is a lack of suitable analytical methods for the determination of active compounds in health supplements such as peptides. The present work describes an implementation of contactless conductivity detection on microchip technology as a new strategy for the electrophoretic determination of L-carnosine in complex health supplement formulations without pre-concentration and derivatization steps. The best results were obtained in the case of +1.00 kV applied for 20 s for injection and +2.75 kV applied for 260 s for the separation step. Under the selected conditions, a linear detector response of 5 × 10−6 to 5 × 10−5 M was achieved. L-carnosine retention time was 61 s. The excellent reproducibility of both migration time and detector response confirmed the high precision of the method. The applicability of the method was demonstrated by the determination of L-carnosine in three different samples of health supplements. The recoveries ranged from 91 to 105%. Subsequent analysis of the samples by CE-UV-VIS and HPLC-DAD confirmed the accuracy of the obtained results. LA - English DB - MTMT ER - TY - CONF AU - Balogh-Hartmann, Fruzsina AU - Páger, Csilla AU - Bufa, Anita AU - Madarászné Horváth, Ibolya AU - Marosvölgyi, Tamás AU - Makszin, Lilla TI - Microfluidic-based protein quantification in plant- based drinks derived from nuts T2 - 13th Balaton Symposium on High-Performance Separation Methods Book of Abstracts PY - 2023 PG - 1 UR - https://m2.mtmt.hu/api/publication/34186807 ID - 34186807 LA - English DB - MTMT ER - TY - CONF AU - Balogh-Hartmann, Fruzsina AU - Páger, Csilla AU - Bufa, Anita AU - Madarászné Horváth, Ibolya AU - Marosvölgyi, Tamás AU - Makszin, Lilla TI - Microfluidic analysis for determination of the protein content in legume-based plant drinks T2 - 13th Balaton Symposium on High-Performance Separation Methods Book of Abstracts PY - 2023 UR - https://m2.mtmt.hu/api/publication/34186786 ID - 34186786 LA - English DB - MTMT ER - TY - JOUR AU - Balogh-Hartmann, Fruzsina AU - Páger, Csilla AU - Bufa, Anita AU - Madarászné Horváth, Ibolya AU - Verzár, Zsófia AU - Marosvölgyi, Tamás AU - Makszin, Lilla TI - Microfluidic Analysis for the Determination of Protein Content in Different Types of Plant-Based Drinks JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 18 PG - 19 SN - 1420-3049 DO - 10.3390/molecules28186684 UR - https://m2.mtmt.hu/api/publication/34167332 ID - 34167332 N1 - Institute of Bioanalysis, Medical School, Szentágothai Research Center, University of Pécs, Pécs, 7622, Hungary Institute of Nutritional Sciences and Dietetics, Faculty of Health Sciences, University of Pécs, Pécs, 7621, Hungary Export Date: 9 October 2023 CODEN: MOLEF Correspondence Address: Makszin, L.; Institute of Bioanalysis, Hungary; email: lilla.makszin@aok.pte.hu AB - The widespread consumption of plant-based drinks, driven by health and dietary reasons (including cow's milk allergy, lactose intolerance, milk protein intolerance, following a vegetarian or vegan diet) necessitates automated and accurate test methods. Our study demonstrates the simultaneous determination of protein components and total protein concentrations in plant-based milk alternatives using a rapid and reproducible microchip gel electrophoretic method. As expected, the electrophoretic profiles of each plant-based drink differed. Based on our analyses and statistical evaluation, it can be determined that the protein profiles of different plant-based beverages do not differ significantly between different manufacturers or different expiry dates. The measured total protein content was compared with the nominal values, i.e., the values stated on the beverage labels. As the number of consumers of functional and specialized plant-based milk alternatives continues to rise, it is important to prioritize methods that provide qualitative and quantitative information on protein composition and other nutrients. LA - English DB - MTMT ER - TY - CHAP AU - Pukleš, Iva AU - Páger, Csilla AU - Nikola, Sakač AU - Brunislav, Matasović AU - Bojan, Šarkanj AU - Katarína, Molnárov AU - Vesigne, Ana AU - Jozanović, Marija ED - Kajos, Luca Fanni ED - Bali, Cintia ED - Puskás, Tamás ED - Polgár, Petra Ibolya ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám ED - Kovács, Eszter TI - Different electrophoretic approaches in the determination of L-Carnosine in commercial dietary supplement formulations T2 - XI. Interdiszciplináris Doktorandusz Konferencia 2022. november 25-26 = 11th Interdisciplinary Doctoral Conference 25-26th of November 2022 PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat CY - Pécs SN - 9789636260699 PY - 2022 SP - 201 UR - https://m2.mtmt.hu/api/publication/34050312 ID - 34050312 LA - English DB - MTMT ER - TY - CHAP AU - Patyi, Gábor AU - Páger, Csilla AU - Sándor, Viktor AU - Kiss, Ibolya ED - Karádi, Krisztina Anna ED - Kocsis, Marianna ED - Takács, Dóra ED - Árok, Zsófia TI - LIPOPEPTIDEK VIZSGÁLATA CE-MS MÓDSZERREL VÍZMENTES KÖZEGBEN T2 - XLIV. Kémiai Előadói Napok PB - Magyar Kémikusok Egyesülete (MKE) CY - Szeged CY - Budapest SN - 9786156018090 PY - 2021 SP - 22 UR - https://m2.mtmt.hu/api/publication/34050384 ID - 34050384 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Patyi, Gábor AU - Páger, Csilla AU - Sándor, Viktor TI - Ciklikus lipopeptidek vizsgálata CE-MS/MS módszerrel vízmentes közegben T2 - METT25 a Magyar Elválasztástudományi Társaság jubileumi konferenciája PB - Magyar Elválasztástudományi Társaság CY - Pécs SN - 9786155270666 PY - 2021 UR - https://m2.mtmt.hu/api/publication/33603585 ID - 33603585 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Mező, Emerencia AU - Bufa, Anita AU - Páger, Csilla AU - Poór, Viktória AU - Marosvölgyi, Tamás AU - Kilár, Ferenc AU - Makszin, Lilla TI - The Role of Ionic Liquid Interaction in the Separation of Fatty Acid Methyl Esters—Polyunsaturated Geometric Isomers in GC–MS JF - SEPARATIONS J2 - SEPARATIONS VL - 8 PY - 2021 IS - 4 PG - 16 SN - 2297-8739 DO - 10.3390/separations8040038 UR - https://m2.mtmt.hu/api/publication/31933042 ID - 31933042 AB - Knowledge of the type and level of saturated and unsaturated fatty acids in food and clinical matrices is of practical importance, but the wide variety of fatty acids makes analyses very complex. The discrimination of the geometric isomers of fatty acid needs proper and effective separation conditions. The efficiency of three different stationary phases was evaluated by GC–MS methods in the separation of fatty acids in their methyl ester forms. Significant differences were observed in the efficiencies of polysiloxane-based (non-polar HP-5MS and medium/high polarity DB-225MS) and ionic liquid-based (SLB-IL111) columns. Baseline separation of the geometric isomers of linoleic acid methyl ester was obtained by the extremely polar SLB-IL111 column, showing a preference over the other two columns. The optimization of the experimental conditions (response linearity, limit of detection, limit of quantification, system suitability, intraday and interday repeatability and accuracy) showed the separation power of the ionic liquid interaction in the analyses by using short (25–30 m long) columns. By deducting the general principles of the interaction, predictions can be made for the separation of other isomers. The results facilitate the precise identification of various types of fatty acids in real samples for nutritional information. LA - English DB - MTMT ER -