TY - JOUR AU - Pápa, Zsuzsanna AU - Sándor, Péter AU - Lovász, Béla AU - Budai, Judit AU - Kasza, József AU - Márton, Zsuzsanna AU - Jójárt, Péter AU - Seres, Imre AU - Bengery, Zsolt Gábor AU - Németh, Csaba AU - Dombi, Péter AU - Rácz, Péter TI - Control of plasmonic field enhancement by mode-mixing JF - APPLIED PHYSICS LETTERS J2 - APPL PHYS LETT VL - 120 PY - 2022 IS - 5 PG - 5 SN - 0003-6951 DO - 10.1063/5.0072168 UR - https://m2.mtmt.hu/api/publication/32836988 ID - 32836988 LA - English DB - MTMT ER - TY - JOUR AU - Lőrincz, András AU - Mihály, Judith AU - Wacha, András Ferenc AU - Németh, Csaba AU - Besztercei, Balázs AU - Gyulavári, Pál AU - Varga, Zoltán AU - Peták, István AU - Bóta, Attila TI - Combination of multifunctional ursolic acid with kinase inhibitors for anti-cancer drug carrier vesicles JF - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS J2 - MAT SCI ENG C-MATER VL - 131 PY - 2021 PG - 11 SN - 0928-4931 DO - 10.1016/j.msec.2021.112481 UR - https://m2.mtmt.hu/api/publication/32461679 ID - 32461679 N1 - Research Centre for Natural Sciences - Eötvös Loránd Research Network, Institute of Materials and Environmental Chemistry, Research Group of Biological Nanochemistry, Magyar tudósok boulevard 2, Budapest, 1117, Hungary Semmelweis University, Institute of Clinical Experimental Research, Tűzoltó street 37-47, Budapest, 1094, Hungary Semmelweis University, Pathobiochemistry Research Group, Tűzoltó street 37-47, Budapest, 1094, Hungary University of Illinois at Chicago, Department of Biopharmaceutical Sciences, 833 S. Wood street, Chicago, IL 60612, United States Oncompass Medicine Ltd., Retek street 34, Budapest, 1024, Hungary Semmelweis University, Department of Pharmacology and Pharmacotherapy, Nagyvárad square 4, Budapest, 1089, Hungary Export Date: 26 October 2021 Correspondence Address: Mihály, J.; Research Centre for Natural Sciences - Eötvös Loránd Research Network, Magyar tudósok boulevard 2, Hungary; email: mihaly.judith@ttk.hu LA - English DB - MTMT ER - TY - JOUR AU - Mink, János AU - Staiger, Lena AU - Muhr, Maximilian AU - Gemel, Christian AU - Drees, Markus AU - Hajba, László AU - Mihály, Judith AU - Németh, Csaba AU - Lokshin, Boris V AU - Hemmer, Karina AU - Schutz, Max AU - Cokoja, Mirza AU - Fischer, Roland A. TI - Structural studies of ligand stabilized Ni/Ga clusters by means of vibrational spectroscopy and theoretical calculations JF - JOURNAL OF RAMAN SPECTROSCOPY J2 - J RAMAN SPECTROSC VL - 52 PY - 2021 IS - 12 SP - 2317 EP - 2337 PG - 21 SN - 0377-0486 DO - 10.1002/jrs.6199 UR - https://m2.mtmt.hu/api/publication/32431325 ID - 32431325 N1 - Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary Department of Chemistry, Technische Universität München, Garching bei München, Germany Catalysis Research Center, Technische Universität München, Garching bei München, Germany Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, Faculty of Engineering, University of Pannonia, Veszprém, Hungary A.N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russian Federation Cited By :2 Export Date: 18 November 2022 CODEN: JRSPA Correspondence Address: Hajba, L.; Translational Glycomics Research Group, Hungary; email: hajba@mukki.richem.hu AB - In order to explain the different catalytic activity in hydrogenation of two new intermetallic Ni/Ga clusters, [Ga-7](NiCp*)(6) (1A) and [NiGa6](NiCp*)(6) (1B) (Cp* = C5Me5), investigations of structure-function relationship have been performed based on Raman and infrared (IR) spectroscopy and theoretical (density functional theory [DFT] and normal coordinate) calculations. Full interpretation of the Raman, far-IR, and mid-IR spectra of these dark colored solids has been proposed. Based on the overview of metal-Cp* complexes, all the 14 characteristic Cp*(-) skeletal fundamental modes have been identified. By comparison of the Ni-Cp* stretching and tilting external modes (350-380 cm(-1)), their force constants, and bond lengths, cluster 1B exhibited slightly stronger metal-ligand bonding. Vibrations of Ga-7 and NiGa6 cluster cores showed that the stretching wavenumbers and force constants of Ni-Ga (100-350 cm(-1)) and Ga-Ga (60-250 cm(-1)) bonds are higher for cluster 1B, in agreement with the shorter averaged experimental and calculated bond lengths of Ga-Ga bonds (2.873 and 2823 angstrom for clusters 1A and 1B, respectively). Cluster hydrogenation experiments with H-2 and D-2 showed strong Ni-H and N-D stretching features (at 1750 and 1260 cm(-1), respectively), and at the same time, characteristic bands of self-hydrogenated Cp*H and remained nondegraded clusters have been detected. The extent of H-D exchange in cluster deuteration was obtained about 1.5 times more effective with cluster 1B than cluster 1A. The stronger hydrogen or deuterium uptake by cluster 1B and the more intensive self-hydrogenation of Cp* clearly support the higher hydrogenation activity of cluster 1B compared with that of 1A. LA - English DB - MTMT ER - TY - JOUR AU - Tálas, Emília AU - Szőllősi, György AU - Kristyán, Sándor AU - Németh, Csaba AU - Firkala, Tamás AU - Mink, János AU - Mihály, Judith TI - Surface enhanced Raman spectroscopic (SERS) behavior of phenylpyruvates used in heterogeneous catalytic asymmetric cascade reaction JF - SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY J2 - SPECTROCHIM ACTA A VL - 260 PY - 2021 PG - 10 SN - 1386-1425 DO - 10.1016/j.saa.2021.119912 UR - https://m2.mtmt.hu/api/publication/32035457 ID - 32035457 N1 - Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Eötvös Loránd Research Network (ELKH), H-1117 Budapest, Magyar Tudósok körútja 2, Hungary MTA-SZTE Stereochemistry Research Group, H-6720 Szeged, Dóm tér 8, Hungary Department of Chemistry, Eszterházy Károly University, H-3300 Eger, Leányka u. 6, Hungary Export Date: 6 December 2022 CODEN: SAMCA Correspondence Address: Tálas, E.H-1519 Budapest, P.O. Box 286, Hungary; email: talas.emilia@ttk.hu Funding details: European Commission, EC Funding details: European Regional Development Fund, ERDF, Em?lia T?las Funding details: National Research, Development and Innovation Office, K131594 Funding text 1: The authors would like to express their thanks to Lenke Kovács for the preparation of phenylpyruvate derivatives. The funding by the National Research, Development and Innovation Office NKFIH under grant number K131594 is also acknowledged (Judith Mihály). The research within project No.VEKOP-2.3.2-16-2017-00013 was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund (Emília Tálas). Funding text 2: The authors would like to express their thanks to Lenke Kov?cs for the preparation of phenylpyruvate derivatives. The funding by the National Research, Development and Innovation Office NKFIH under grant number K131594 is also acknowledged (Judith Mih?ly). The research within project No.VEKOP-2.3.2-16-2017-00013 was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund (Em?lia T?las). LA - English DB - MTMT ER - TY - JOUR AU - Lendvay, György AU - Majzik, Eszter AU - Nagyné Bereczki, Laura AU - Domján, Attila AU - Trif, László AU - Sajó, István AU - Paiva Franguelli, Fernanda AU - Farkas, Attila AU - Klébert, Szilvia AU - Bombicz, Petra AU - Németh, Csaba AU - Szilágyi, Imre Miklós AU - Kótai, László TI - (Me 2 NH 2 ) 10 [H 2 -Dodecatungstate] polymorphs: dodecatungstate cages embedded in a variable dimethylammonium cation + water of crystallization matrix JF - RSC ADVANCES J2 - RSC ADV VL - 11 PY - 2021 IS - 6 SP - 3713 EP - 3724 PG - 12 SN - 2046-2069 DO - 10.1039/d0ra09997j UR - https://m2.mtmt.hu/api/publication/31819362 ID - 31819362 N1 - Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, H-1117, Hungary University of Technology and Economics, Department of Inorganic and Analytical Chemistry, Budapest, H-1111, Hungary University of Pécs, János Szentágothai Research Centre, Pécs, H-7624, Hungary University of Technology and Economics, Department of Organic Chemistry, Budapest, H-1111, Hungary Deuton-X Ltd., Selmeci u. 89, Érd, H-2030, Hungary Export Date: 11 March 2021 CODEN: RSCAC Correspondence Address: Kótai, L.; Institute of Materials and Environmental Chemistry, Hungary; email: kotai.laszlo@ttk.mta.hu Correspondence Address: Kótai, L.; Deuton-X Ltd., Selmeci u. 89, Hungary; email: kotai.laszlo@ttk.mta.hu Funding details: K-115762, K-124544 Funding details: BME IE-NAT TKP2020 Funding details: European Commission, EC Funding details: Magyar Tudományos Akadémia, MTA, ÚNKP-18-4-BME-238 Funding details: European Regional Development Fund, FEDER Funding text 1: The research within projects No. VEKOP-2.3.2-16-2017-00013 and GINOP-2.2.1-15-2017-00084 was supported by the European Union and the State of Hungary, co-nanced by the European Regional Development Fund. N. V. M. and P. B. are grateful for grants by the Hungarian Scientic Research Found (K-124544 and K-115762). The K-124212 and an NRDI TNN_16 123631 grants are also acknowledged. I. M. S. thanks the Hungarian Academy of Sciences for a János Bolyai Research Fellowship and acknowledges the ÚNKP-18-4-BME-238 grant supported by the New National Excellence Program of the Ministry of Human Capacities, Hungary. An NRDI K 124212 and an NRDI TNN_16 123631 grants are acknowledged. The research reported in this paper was supported by the BME Nanotechnology and Materials Science TKP2020 IE grant of NKFIH Hungary (BME IE-NAT TKP2020). Funding Agency and Grant Number: European UnionEuropean Commission [GINOP-2.2.1-15-2017-00084, VEKOP-2.3.2-16-2017-00013]; State of Hungary; European Regional Development FundEuropean Commission; Hungarian Scientific Research FoundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [K-124544, K-115762]; NRDI [K 124212, TNN_16 123631]; Hungarian Academy of SciencesHungarian Academy of Sciences; New National Excellence Program of the Ministry of Human Capacities, Hungary [uNKP-18-4-BME-238]; BME Nanotechnology and Materials Science TKP2020 IE grant of NKFIH Hungary (BME IE-NAT TKP2020); [K-124212]; [NRDI TNN_16 123631] Funding text: The research within projects No. VEKOP-2.3.2-16-2017-00013 and GINOP-2.2.1-15-2017-00084 was supported by the European Union and the State of Hungary, co-financed by the European Regional Development Fund. N. V. M. and P. B. are grateful for grants by the Hungarian Scientific Research Found (K-124544 and K-115762). The K-124212 and an NRDI TNN_16 123631 grants are also acknowledged. I. M. S. thanks the Hungarian Academy of Sciences for a Janos Bolyai Research Fellowship and acknowledges the uNKP-18-4-BME-238 grant supported by the New National Excellence Program of the Ministry of Human Capacities, Hungary. An NRDI K 124212 and an NRDI TNN_16 123631 grants are acknowledged. The research reported in this paper was supported by the BME Nanotechnology and Materials Science TKP2020 IE grant of NKFIH Hungary (BME IE-NAT TKP2020). LA - English DB - MTMT ER - TY - JOUR ED - Eszter, Majzik / Collaborator AU - Paiva Franguelli, Fernanda AU - Lendvay, György AU - Trif, László AU - Németh, Csaba AU - Farkas, Attila AU - Klébert, Szilvia AU - Nagyné Bereczki, Laura AU - Szilágyi, Imre Miklós AU - Kótai, László TI - Deuteration and Vibrational Spectra of Dimethylammonium Paratungstate-B hydrates JF - ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE J2 - Z ANORG ALLG CHEM VL - 647 PY - 2021 IS - 6 SP - 593 EP - 598 PG - 6 SN - 0044-2313 DO - 10.1002/zaac.202000283 UR - https://m2.mtmt.hu/api/publication/31602701 ID - 31602701 LA - English DB - MTMT ER - TY - JOUR AU - Szentirmai, Veronika AU - Wacha, András Ferenc AU - Németh, Csaba AU - Kitka, Diána AU - Rácz, Anita AU - Héberger, Károly AU - Mihály, Judith AU - Varga, Zoltán TI - Reagent-free total protein quantification of intact extracellular vesicles by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy JF - ANALYTICAL AND BIOANALYTICAL CHEMISTRY J2 - ANAL BIOANAL CHEM VL - 412 PY - 2020 IS - 19 SP - 4619 EP - 4628 PG - 10 SN - 1618-2642 DO - 10.1007/s00216-020-02711-8 UR - https://m2.mtmt.hu/api/publication/31344066 ID - 31344066 N1 - Funding Agency and Grant Number: Research Centre for Natural Sciences; National Research, Development and Innovation Office NKFIH, Hungary [NVKP_16-1-2016-0007, PD 121326, PD 124451, K 131594, K 119269]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-19-3] Funding text: Open access funding provided by Research Centre for Natural Sciences. This study was funded by the National Research, Development and Innovation Office NKFIH, Hungary under grant numbers NVKP_16-1-2016-0007, PD 121326 (Zoltan Varga), PD 124451 (Andras Wacha), K 131594 (Judith Mihaly) and K 119269 (Anita Racz and Karoly Heberger). Zoltan Varga was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. Diana Kitka was supported by the UNKP-19-3 New National Excellence Program of the Ministry for Innovation and Technology. Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Plasma Chemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Export Date: 13 June 2020 CODEN: ABCNB Correspondence Address: Mihály, J.; Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Hungary; email: mihaly.judith@ttk.mta.hu Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Plasma Chemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Export Date: 5 August 2020 CODEN: ABCNB Correspondence Address: Mihály, J.; Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Hungary; email: mihaly.judith@ttk.mta.hu Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Plasma Chemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Export Date: 23 November 2020 CODEN: ABCNB Correspondence Address: Mihály, J.; Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Hungary; email: mihaly.judith@ttk.mta.hu Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Plasma Chemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary Export Date: 22 March 2021 CODEN: ABCNB Correspondence Address: Mihály, J.; Biological Nanochemistry Research Group, Magyar tudósok körútja 2, Hungary; email: mihaly.judith@ttk.mta.hu AB - Extracellular vesicles (EVs) are lipid bilayer-bounded particles that are actively synthesized and released by cells. The main components of EVs are lipids, proteins, and nucleic acids and their composition is characteristic to their type and origin, and it reveals the physiological and pathological conditions of the parent cells. The concentration and protein composition of EVs closely relate to their functions; therefore, total protein determination can assist in EV-based diagnostics and disease prognosis. Here, we present a simple, reagent-free method based on attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to quantify the protein content of EV samples without any further sample preparation. After calibration with bovine serum albumin, the protein concentration of red blood cell-derived EVs (REVs) were investigated by ATR-FTIR spectroscopy. The integrated area of the amide I band was calculated from the IR spectra of REVs, which was proportional to the protein quantity in the sample, regardless of its secondary structure. A spike test and a dilution test were performed to determine the ability to use ATR-FTIR spectroscopy for protein quantification in EV samples, which resulted in linearity with R-2 values as high as 0.992 over the concentration range of 0.08 to 1 mg/mL. Additionally, multivariate calibration with the partial least squares (PLS) regression method was carried out on the bovine serum albumin and EV spectra. R-2 values were 0.94 for the calibration and 0.91 for the validation set. The results indicate that ATR-FTIR measurements provide a reliable method for reagent-free protein quantification of EVs. LA - English DB - MTMT ER - TY - JOUR AU - Quemé Peña, Mayra AU - Juhász, Tünde AU - Mihály, Judith AU - Szigyártó, Imola Csilla AU - Horváti, Kata AU - Bősze, Szilvia AU - Henczkó, Judit AU - Pályi, Bernadett AU - Németh, Csaba AU - Varga, Zoltán AU - Zsila, Ferenc AU - Beke-Somfai, Tamás TI - Manipulating active structure and function of cationic antimicrobial peptide CM15 by the polysulfonated drug suramin: a step closer to in vivo complexity JF - CHEMBIOCHEM J2 - CHEMBIOCHEM VL - 20 PY - 2019 IS - 12 SP - 1578 EP - 1590 PG - 13 SN - 1439-4227 DO - 10.1002/cbic.201800801 UR - https://m2.mtmt.hu/api/publication/30432275 ID - 30432275 N1 - Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, 1117, Hungary National Biosafety Laboratory, National Public Health Center, Albert Flórián út 2, Budapest, 1097, Hungary Export Date: 4 June 2019 CODEN: CBCHF Correspondence Address: Juhász, T.; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Hungary; email: juhasz.tunde@ttk.mta.hu Funding Agency and Grant Number: Momentum Program [LP2016-2]; National Competitiveness and Excellence Program [NVKP_16-1-2016-0007]; GINOP [BIONA-NO_GINOP-2.3.2-15-2016-00017]; National Research Development and Innovation Office, Hungary [OTKA 104275, 115431, 124077]; European Union [VEKOP-2.3.3-15-2017-00020, VEKOP-2.3.2-16-2017-00014]; State of Hungary [VEKOP-2.3.3-15-2017-00020, VEKOP-2.3.2-16-2017-00014]; European Regional Development Fund; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; Hungarian Ministry of Human Capacities [783-3/2018/FEKUTSRAT] Funding text: This work was supported through grants provided by the Momentum Program (LP2016-2), the National Competitiveness and Excellence Program (NVKP_16-1-2016-0007) and GINOP (BIONA-NO_GINOP-2.3.2-15-2016-00017). We also thank the National Research Development and Innovation Office, Hungary (grants OTKA 104275, 115431, 124077) and additionally are grateful for grants (VEKOP-2.3.3-15-2017-00020, VEKOP-2.3.2-16-2017-00014) from the European Union and the State of Hungary, co-financed by the European Regional Development Fund. K.H. was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. Sz.B. thanks the ELTE Institutional Excellence Program (783-3/2018/FEKUTSRAT) supported by the Hungarian Ministry of Human Capacities. Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Budapest, 1117, Hungary MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, 1117, Hungary National Biosafety Laboratory, National Public Health Center, Albert Flórián út 2, Budapest, 1097, Hungary Export Date: 10 July 2019 CODEN: CBCHF Correspondence Address: Juhász, T.; Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, Hungary; email: juhasz.tunde@ttk.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Mink, János AU - Stirling, András AU - Dickson, O Ojwang AU - Gunnar, Svensson AU - Mihály, Judith AU - Németh, Csaba AU - Markus, Drees AU - Hajba, László TI - Vibrational properties and bonding analysis of copper hexacyanoferrate complexes in solid state. Latest articles TS - Latest articles JF - APPLIED SPECTROSCOPY REVIEWS J2 - APPL SPECTROSC REV VL - 54 PY - 2019 IS - 5 SP - 369 EP - 424 PG - 56 SN - 0570-4928 DO - 10.1080/05704928.2018.1459659 UR - https://m2.mtmt.hu/api/publication/3369818 ID - 3369818 N1 - Institute of Materials and Environmental Chemistry, Research Center of Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Research Institute for Biomolecular and Chemical Engineering, University of Pannonia, Veszprém, Hungary Institute of Organic Chemistry, Research Center of Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden Inorganic Chemistry, Catalytic Research Center, Technical University Munich, Garching, Germany Cited By :6 Export Date: 18 November 2022 CODEN: APSRB Correspondence Address: Mink, J.; Institute of Materials and Environmental Chemistry, Magyar tudódok körútja 2., Hungary; email: mink.janos@ttk.mta.hu LA - English DB - MTMT ER - TY - JOUR AU - Juhász, Tünde AU - Mihály, Judith AU - Kohut, Gergely AU - Németh, Csaba AU - Liliom, Károly AU - Beke-Somfai, Tamás TI - The lipid mediator lysophosphatidic acid induces folding of disordered peptides with basic amphipathic character into rare conformations JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 8 PY - 2018 PG - 15 SN - 2045-2322 DO - 10.1038/s41598-018-32786-4 UR - https://m2.mtmt.hu/api/publication/30318630 ID - 30318630 AB - Membrane-active, basic amphipathic peptides represent a class of biomolecules with diverse functions. Sequentially close protein segments also show similar behaviour in several ways. Here we investigated the effect of the lipid mediator lysophosphatidic acid (LPA) on the conformation of structurally disordered peptides including extracellular antimicrobial peptides (AMPs), and calmodulin-binding motifs derived from cytosolic and membrane target proteins. The interaction with associated LPA resulted in gain of ordered secondary structure elements, which for most cases were previously uncharacteristic of the particular peptide. Results revealed mechanism of the LPA-peptide interactions with regulation of the lipid on peptide conformation and oligomerization in a concentration-dependent manner involving (1) relocation of tryptophan residues into the lipid cluster, (2) multiple contacts between the binding partners dictated by complex driving forces, (3) multiple peptide binding to LPA associates with an affinity in the low micromolar range, and (4) selectivity for LPA compared with structurally related lipids. In line with recent findings showing endogenous molecules inducing structural changes in AMPs, we propose that accumulation of LPA in signalling or pathological processes might modulate host-defense activity or trigger certain processes by direct interaction with cationic amphipathic peptide sequences. LA - English DB - MTMT ER -