TY - JOUR AU - Balázs, Benigna AU - Boros, Ákos AU - Pankovics, Péter AU - Nagy, Gábor AU - Szekeres, Sándor AU - Urbán, Péter AU - Reuter, Gábor TI - Detection and complete genome characterization of a genogroup X (GX) sapovirus (family Caliciviridae) from a golden jackal (Canis aureus) in Hungary JF - ARCHIVES OF VIROLOGY J2 - ARCH VIROL VL - 169 PY - 2024 IS - 5 PG - 8 SN - 0304-8608 DO - 10.1007/s00705-024-06034-2 UR - https://m2.mtmt.hu/api/publication/34796272 ID - 34796272 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office [NKFIH FK134311]; University of Pecs Funding text: Funding was provided by the National Research, Development and Innovation Office (NKFIH FK134311).Open access funding provided by University of Pecs. AB - In this study, a novel genotype of genogroup X (GX) sapovirus (family Caliciviridae ) was detected in the small intestinal contents of a golden jackal ( Canis aureus ) in Hungary and characterised by viral metagenomics and next-generation sequencing techniques. The complete genome of the detected strain, GX/Dömsöd/DOCA-11/2020/HUN (PP105600), is 7,128 nt in length. The ORF1- and ORF2-encoded viral proteins (NSP, VP1, and VP2) have 98%, 95%, and 88% amino acid sequence identity to the corresponding proteins of genogroup GX sapoviruses from domestic pigs, but the nucleic acid sequence identity values for their genes are significantly lower (83%, 77%, and 68%). During an RT-PCR-based epidemiological investigation of additional jackal and swine samples, no other GX strains were detected, but a GXI sapovirus strain, GXI/Tótfalu/WBTF-10/2012/HUN (PP105601), was identified in a faecal sample from a wild boar ( Sus scrofa ). We report the detection of members of two likely underdiagnosed groups of sapoviruses (GX and GXI) in a golden jackal and, serendipitously, in a wild boar in Europe. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Fruzsina AU - Gáspár, Gábor AU - Pankovics, Péter AU - Urbán, Péter AU - Herczeg, Róbert AU - Albert, Mihály AU - Reuter, Gábor AU - Boros, Ákos TI - Co-infecting viruses of species Bovine rhinitis B virus (Picornaviridae) and Bovine nidovirus 1 (Tobaniviridae) identified for the first time from a post-mortem respiratory sample of a sheep (Ovis aries) in Hungary JF - INFECTION GENETICS AND EVOLUTION J2 - INFECT GENET EVOL VL - 120 PY - 2024 PG - 8 SN - 1567-1348 DO - 10.1016/j.meegid.2024.105585 UR - https://m2.mtmt.hu/api/publication/34757150 ID - 34757150 N1 - Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary János Szentágothai Research Centre of the University of Pécs, Bioinformatics Research Group, Genomics and Bioinformatics Core Facility, Pécs, Hungary Ceva Phylaxia Ltd. Budapest, Hungary Export Date: 12 April 2024 CODEN: IGENC Correspondence Address: Boros, Á.; Department of Medical Microbiology and Immunology, H-7624, Szigeti út 12, Hungary; email: boros.akos@pte.hu AB - In this study, a picornavirus and a nidovirus were identified from a single available nasopharyngeal swab (NPS) sample of a freshly deceased sheep, as the only vertebrate viruses found with viral metagenomics and next-generation sequencing methods. The sample was originated from a mixed feedlot farm in Hungary where sheep and cattle were held together but in separate stalls. Most of the sheep had respiratory signs (coughing and increased respiratory effort) at the time of sampling. Other NPS were not, but additional enteric samples were collected from sheep (n = 27) and cattle (n = 11) of the same farm at that time. The complete/nearly complete genomes of the identified viruses were determined using RT-PCR and Nanopore (MinION-Flonge) / Dye-terminator sequencing techniques. The results of detailed genomic and phylogenetic analyses indicate that the identified picornavirus most likely belongs to a type 4 genotype of species Bovine rhinitis B virus (BRBV-4, OR885914) of genus Aphthovirus, family Picornaviridae while the ovine nidovirus (OvNV, OR885915) - as a novel variant - could belong to the recently created Bovine nidovirus 1 (BoNV) species of genus Bostovirus, family Tobaniviridae. None of the identified viruses were detectable in the enteric samples using RT-PCR and generic screening primer pairs. Both viruses are well-known respiratory pathogens of cattle, but their presence was not demonstrated before in other animals, like sheep. Furthermore, neither BRBV-4 nor BoNVs were investigated in European cattle and/or sheep flocks, therefore it cannot be determined whether the presence of these viruses in sheep was a result of a single host species switch/spillover event or these viruses are circulating in not just cattle but sheep populations as well. Further studies required to investigate the spread of these viruses in Hungarian and European sheep and cattle populations and to identify their pathogenic potential in sheep. LA - English DB - MTMT ER - TY - JOUR AU - Davies, Hannah AU - Dastjerdi, Akbar AU - Everest, David AU - Floyd, Tobias AU - Collins, Rachael AU - McFadzean, Harriet AU - Reuter, Gábor AU - Reichel, Rudolf TI - Incidental finding of a human-like tusavirus in a lamb with lip lesions and fatal pneumonia JF - JOURNAL OF GENERAL VIROLOGY J2 - J GEN VIROL VL - 105 PY - 2024 IS - 3 PG - 8 SN - 0022-1317 DO - 10.1099/jgv.0.001968 UR - https://m2.mtmt.hu/api/publication/34729562 ID - 34729562 N1 - Export Date: 03 May 2024; CODEN: JGVIA AB - Tusaviruses in the genus Protoparvovirus of family Parvoviridae were first identified in a diarrhoeic Tunisian child in 2014. Thereafter, high prevalence of a genetically similar virus was demonstrated in faeces from caprine and ovine species in Hungary. Here, we describe an investigation into the cause of scabby lip lesions in a 6 month-old lamb, submitted from a farm experiencing weight loss and scouring in lambs in England. Transmission electron microscopy visualised small circular particles of 18 and 22 nm in diameter in lip lesions identified as tusavirus and flumine parvovirus by Next Generation Sequencing. Liver, kidney, lung, small intestine content and faeces were also strongly positive for the tusavirus DNA as well as 10 % of faecal samples of the flock collected 2 months after the initial lip sampling. NS1 and VP1 amino acid sequences of this tusavirus displayed 99.5 and 92.89 % identity to those of a human tusavirus, respectively. These amino acid identities were at 95.5 and 89.68 % when compared to those of a goat tusavirus. Phylogenetic analysis of the NS1 and VP1 also grouped the virus in the genus Protoparvovirus and close to tusaviruses detected in human, ovine and caprine species. Wider surveillance of the virus indicated a broader geographical distribution for the virus in England. Histology of the lip tissue revealed localised areas of epidermal hyperplasia and hyperkeratosis affecting haired skin, with mild leucocyte infiltration of the subjacent dermis, but no changes to implicate virus involvement. Flumine parvovirus was concluded to be an environment contaminant. Broader studies in prevalence of these virus in UK sheep flocks and human population, animal models and experimental infections could provide insights into the pathogenesis of these novel viruses and their zoonotic potential. LA - English DB - MTMT ER - TY - JOUR AU - Németh, Csongor AU - Boros, Ákos AU - Mészáros, Endre AU - Gyömörei, Csaba AU - Albert, Ervin AU - Pankovics, Péter AU - Reuter, Gábor TI - Human orf virus (family Poxviridae) infection following a lamb bite in Hungary JF - ARCHIVES OF VIROLOGY J2 - ARCH VIROL VL - 169 PY - 2024 IS - 3 PG - 5 SN - 0304-8608 DO - 10.1007/s00705-024-06002-w UR - https://m2.mtmt.hu/api/publication/34725854 ID - 34725854 N1 - Case Reports; Journal Article AB - Human orf disease (called ecthyma contagiosum or contagious/infectious pustular dermatitis in animals) was confirmed on the fingers of both hands of a 24-year-old female, after feeding diseased lambs with a nursing bottle in April 2023. In addition to skin symptoms, she had low-grade fever (37.6°C) and swollen lymph nodes in both axilla. The presence of orf virus (genus Parapoxvirus, family Poxviridae) was confirmed, and this strain, Baja/2023/HUN (OR372161-OR372163), was found to have > 98% nucleotide sequence identity to sheep-origin orf viruses in four tested genome regions (ORF011/B2L, ORF019, ORF020/VIR, and ORF056). This is the first report of a human case of infection with the neglected zoonotic orf virus in Hungary. LA - English DB - MTMT ER - TY - JOUR AU - Pankovics, Péter AU - Nagy, Arnold AU - Nyul, Zoltán AU - Juhász, Annamária AU - Takáts, Károly AU - Boros, Ákos AU - Reuter, Gábor TI - Human cases of lymphocytic choriomeningitis virus (LCMV) infections in Hungary JF - ARCHIVES OF VIROLOGY J2 - ARCH VIROL VL - 168 PY - 2023 IS - 11 PG - 6 SN - 0304-8608 DO - 10.1007/s00705-023-05905-4 UR - https://m2.mtmt.hu/api/publication/34216028 ID - 34216028 N1 - Case Reports AB - Lymphocytic choriomeningitis (LCM) is a "neglected" rodent-borne viral zoonotic disease caused by lymphocytic choriomeningitis virus (LCMV) (family Arenaviridae). The aim of this retrospective clinical and laboratory study was to detect LCMV RNA, using RT-PCR, in cerebrospinal fluid samples collected from patients with central nervous system (CNS) infections of unknown aetiology from over a 12-year period in Hungary. Between 2009 and 2020, a total of 74 cerebrospinal fluid samples were tested using an in-house LCMV-specific RT-PCR-based method at the Department of Medical Microbiology and Immunology, University of Pécs. The mean age of the 74 patients included in our study was 24 years (min. 5, max. 74), with a predominance of men (44 [59.5%]; women, 30 [40.5%]). Two (2.7%) cerebrospinal fluid samples were found to be positive for LCMV RNA by RT-PCR and sequencing. The first LCMV case was a 5-year-old preschool boy who had a hamster bite on his left-hand finger, and the second LCMV case was a 74-year-old man who was living in a village and had incipient dementia and a previous permanent functional CNS impairment. The two detected LCMV strains (MW558451 and OM648933) from the year 2020 belonged to two different genetic lineages (I and II). These two cases of CNS inflammation of unknown origin represent the first published human LCMV infections confirmed by molecular methods in Hungary. LA - English DB - MTMT ER - TY - JOUR AU - Boros, Ákos AU - Pankovics, Péter AU - László, Zoltán AU - Urbán, Péter AU - Herczeg, Róbert AU - Gáspár, Gábor AU - Tóth, Fruzsina AU - Reuter, Gábor TI - The genomic and epidemiological investigations of enteric viruses of domestic caprine (Capra hircus) revealed the presence of multiple novel viruses related to known strains of humans and ruminant livestock species JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 11 PY - 2023 IS - 6 PG - 28 SN - 2165-0497 DO - 10.1128/spectrum.02533-23 UR - https://m2.mtmt.hu/api/publication/34214746 ID - 34214746 N1 - * Megosztott szerzőség AB - In this study, the enteric virome of diarrheic caprine was investigated using next-generation sequencing, reverse transcription PCR methods, and bioinformatics analyses. The complete or nearly complete genomes of seven novel viruses including (i) a caprine astrovirus (OQ758025) of family Astroviridae, four picornaviruses in genera (ii) Boosepivirus (OQ758026), (iii) Enterovirus (OQ758028), (iv) Kobuvirus (OQ758029), and (v) an unassigned picornavirus provisionally called as capripivirus (from the term caprine picornavirus) of the family Picornaviridae (OQ758027), as well as (vi) a tusavirus of family Parvoviridae (OL692339.2) and an (vii) unassigned CRESS DNA virus (OQ758030) have been identified. Structural analyses of the internal ribosomal entry sites revealed the presence of unique motifs and suggest the modular exchange of certain elements between co-infecting viruses. Epidemiological investigations and genotyping PCR reactions of identified RNA virus groups were also conducted using multiple "in-house" developed SYBRgreen-based screening quantitative PCR (qPCR) assays and generic capsid primers on additional fecal samples from ruminant livestock species (n = 62 caprine, n = 32 ovine, and n = 94 cattle) of three different age groups from n = 12 geographically distant sampling sites in Hungary. The results of qPCR screening assays as well as K-means cluster and phylogenetic analyses of the determined sequences suggest that diverse members of the above RNA virus groups were found in all age groups, but mostly in <1-year-old ruminants. Members of the analyzed virus groups were found frequently (37.2%) in multiple (even in quintuple) co-infections, while strains related closely to the identified index viruses were detectable only in certain caprine and ovine samples indicating a wide range of viral diversity. IMPORTANCE Compared with other domestic animals, the virome and viral diversity of small ruminants especially in caprine are less studied even of its zoonotic potential. In this study, the enteric virome of caprine was investigated in detail using next-generation sequencing and reverse transcription PCR techniques. The complete or nearly complete genomes of seven novel viruses were determined which show a close phylogenetic relationship to known human and ruminant viruses. The high similarity between the identified caprine tusavirus (family Parvoviridae) and an unassigned CRESS DNA virus with closely related human strains could indicate the (reverse) zoonotic potential of these viruses. Others, like astroviruses (family Astroviridae), enteroviruses, or novel caripiviruses (named after the term caprine picornavirus) of family Picornaviridae found mostly in multiple co-infections in caprine and ovine, could indicate the cross-species transmission capabilities of these viruses between small ruminants. LA - English DB - MTMT ER - TY - JOUR AU - Reuter, Gábor AU - Boros, Ákos AU - Pankovics, Péter TI - Molecular epidemiology and characterization of endemic, epidemic and imported hepatitis A virus (HAV) strains in Hungary (2003-2022) JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - 3 SP - 246 EP - 251 PG - 6 SN - 1217-8950 DO - 10.1556/030.2023.02099 UR - https://m2.mtmt.hu/api/publication/34081452 ID - 34081452 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office [K111615, FK134311] Funding text: The authors would like to thank the virology laboratory workers throughout the country for accurately conducting the HAV serological diagnostic tests related to this study. We would express our special thanks to dr Timea Kolozsi (Budapest) , dr Andrea Katai (Szeged) , dr Maria Vollain (Veszprem) , dr & Aacute;gnes Juhasz (Debrecen) , and Laszlone Kosztolanyi (Miskolc) for the representative sample collection. This work was financially supported by grants from the National Research, Development and Innovation Office (K111615 and FK134311) . AB - Hepatitis A virus (HAV) is one of the most important etiological agents of acute viral hepatitis but comprehensive molecular epidemiological study with chrono-phylogeographical data are not available from Hungary.Between 2003 and 2022, a total of 8,307 HAV infections were registered officially in Hungary of which 400 (4.8%) HAV IgM antibody-positive serum samples were collected countrywide. HAV genomic RNA was successfully detected in 216/400 (54%) sera by RT-PCR subsequently confirmed by sequencing. The complete nucleotide sequences of VP1 region were determined in 32 representative HAV strains. Based on the sequence analysis, 150 (69.4%) strains were characterized as HAV sub-genotype IA and 66 (30.6%) as sub-genotype IB, respectively. Based on the combined epidemiological and molecular data, epidemic, endemic, and imported HAV strains were also characterized. The first two registered countrywide outbreaks started among men-sex-with men (MSM) in 2011 (sub-genotype IA) and 2021 (sub-genotype IB), the continuously circulating endemic/domestic HAV strain (sub-genotype IA) in East Hungary and the travel-related sub-genotype IB strains from Egypt should be highlighted. All HAV strains are deposited in the HAVNET database (https://www.rivm.nl/en/havnet).In this 20-year-long comprehensive molecular epidemiological study, we report the genetic characterization and geographic distribution of endemic, epidemic and imported HAV strains for the first time in Hungary with continuous co-circulation of sub-genotypes IA and IB HAV strains since 2003. These data provide basic information about the HAV situation in the country in an international context and can promote more effective national public health intervention strategies for the prevention of HAV transmissions and infections. LA - English DB - MTMT ER - TY - JOUR AU - Reuter, Gábor AU - Boros, Ákos AU - Takáts, Károly AU - Mátics, Róbert AU - Pankovics, Péter TI - A novel mammarenavirus (family Arenaviridae) in hedgehogs (Erinaceus roumanicus) in Europe JF - ARCHIVES OF VIROLOGY J2 - ARCH VIROL VL - 168 PY - 2023 IS - 7 PG - 5 SN - 0304-8608 DO - 10.1007/s00705-023-05804-8 UR - https://m2.mtmt.hu/api/publication/34012527 ID - 34012527 N1 - Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti út 12., Pécs, 7624, Hungary Hungarian Nature Research Society, Ajka, Hungary Cited By :2 Export Date: 11 January 2024 CODEN: ARVID Correspondence Address: Reuter, G.; Department of Medical Microbiology and Immunology, Szigeti út 12., Hungary; email: reuter.gabor@gmail.com AB - In this study, a novel mammarenavirus (family Arenaviridae) was identified in a hedgehog (family Erinaceidae) in Hungary and genetically characterized. Mecsek Mountains virus (MEMV, OP191655, OP191656) was detected in nine (45%) out of 20 faecal specimens collected from a Northern white-breasted hedgehog (Erinaceus roumanicus). The L-segment proteins (RdRp and Z) and S-segment proteins (NP and GPC) of MEMV had 67.5%/70% and 74.6%/65.6% amino acid sequence identity, respectively, to the corresponding proteins of Alxa virus (species Mammarenavirus alashanense) identified recently in an anal swab from a three-toed jerboa (Dipus sagitta) in China. MEMV is the second known arenavirus endemic in Europe. LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Benigna AU - Boros, Ákos AU - Pankovics, Péter AU - Reuter, Gábor TI - High seroprevalence of hepatitis E virus (HEV) in South Transdanubia, Hungary (2010-2022). JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - 2 SP - 119 EP - 125 PG - 7 SN - 1217-8950 DO - 10.1556/030.2023.02010 UR - https://m2.mtmt.hu/api/publication/33826928 ID - 33826928 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office (NKFI) [FK134311] Funding text: The authors would like to thank the virology laboratory workers (Gertrud Domonkos, Zsoltne Garamvoelgyi, Eva Lovas and Csilla Weber) for accurately conducting HEV serological tests related to this study. The study was supported by a grant from National Research, Development and Innovation Office (NKFI, FK134311) . AB - Hepatitis E virus (HEV) is an increasingly recognized etiological agent of acute, chronic and extrahepatic human infections with primarily zoonotic origin in Europe. Limited numbers of comprehensive population-based studies are available related to HEV seroepidemiology, especially from Central Europe.The aim of this study was to investigate the seroprevalence and trends of total and IgM antibodies against HEV in different age groups in the population of South Transdanubia, Hungary, within a thirteen years long period between the years 2010 and 2022.We retrospectively analysed the serological test results of HEV total and HEV IgM antibodies carried out by ELISA technique using Dia.Pro (Diagnostic Bioprobes, Italy) kit from serum samples collected from patients with or without hepatitis between January 1, 2010 and December 31, 2022.The number of tested samples (∑6,996 for total antibody and ∑6,582 for IgM) increased during the study period. The average HEV total and the IgM antibody seropositivities were 33% (2,307/6,996 samples) and 9.6% (642/6,582 samples), respectively, in the study population. The HEV total antibody seropositivity varied in different age groups between 3.9% (age group 1-5 years) and 58.6% (86-90 years) and showed an increasing positivity by age. At the age groups >50 years, nearly half (43%) of the population had antibodies against HEV. The HEV IgM positivity had an increasing trend of up to 13.9% in the age group 81-85 years.High HEV total and IgM antibody seroprevalence were detected in South Transdanubia, Hungary, confirming that this region is highly endemic for HEV infections in Europe. LA - English DB - MTMT ER - TY - JOUR AU - Reuter, Gábor AU - Pankovics, Péter AU - Nagy, Gábor AU - Szekeres, Sándor AU - Boros, Ákos TI - A novel vesivirus (family Caliciviridae) in European badgers (Meles meles) in Hungary, 2020/2021. JF - ARCHIVES OF VIROLOGY J2 - ARCH VIROL VL - 168 PY - 2023 IS - 4 PG - 5 SN - 0304-8608 DO - 10.1007/s00705-023-05733-6 UR - https://m2.mtmt.hu/api/publication/33695781 ID - 33695781 AB - In this study, a novel vesivirus (family Caliciviridae) was detected and characterized in faecal and tissue (blood and spleen) specimens collected from three (23.1%) out of 13 European badgers (Meles meles) in Hungary that were tested using RT-PCR and sequencing methods. The complete genome of the vesivirus strain European badger/B40/2021/HUN (OQ161773) is 8,375 nucleotides in length. The ORF1, ORF2, and ORF3 proteins have 81.1%, 70.5%, and 64.2% amino acid sequence identity, respectively, to the corresponding proteins of Asian badger vesivirus, which was first reported in badgers in China in 2022. These results indicate that more than one lineage/species of vesiviruses circulates in mustelid badgers in geographically different regions. LA - English DB - MTMT ER -