TY - JOUR AU - Dang, Nga Phuong AU - Petrich, Chris AU - Pasztor, Dorina AU - Schneider, György AU - Calay, Rajnish Kaur TI - Shewanella baltica in a Microbial Fuel Cell for Sensing of Biological Oxygen Demand (BOD) of Wastewater JF - ANALYTICAL LETTERS J2 - ANAL LETT PY - 2024 PG - 13 SN - 0003-2719 DO - 10.1080/00032719.2024.2341087 UR - https://m2.mtmt.hu/api/publication/34824870 ID - 34824870 N1 - Department of Cold Climate Technology, SINTEF Narvik, Narvik, Norway Department of Medical Microbiology and Immunology, University of Pécs, Medical School, Pécs, Hungary UiT–The Arctic University of Norway, Tromso, Norway Export Date: 6 May 2024 CODEN: ANALB Correspondence Address: Dang, N.P.; Department of Cold Climate Technology, Norway; email: nga.dang@sintef.no AB - Geobacter and Shewanella are the most characterized electroactive bacteria genera. Unlike genus Geobacter that is strictly anaerobic, Shewanella can grow under both oxic and anoxic environments and is capable of metabolizing a wider substrate range. In the present study, the use of strain Shewanella baltica 20 in MFC-based biosensor for BOD monitoring is reported. The S. baltica strain 20 was isolated from river sediment in Hungary. The bacterium could form a biofilm, oxidized glucose, and transferred electrons to produce current when they were enriched on the anode in an air cathode microbial fuel cell (MFC). The tested MFC system demonstrated linearity in the current response to glucose from 50 to 300 mg/L. The electrical efficiency was determined from the polarization curve using the method of Varying Circuit Resistance (VCR) with an external load probed from 10 ohm to 220 k ohm. The maximum power production was 1.2 mW/m2 at an external load of 40 k ohm. Studying the effect of external resistors on the MFC performance showed that the MFC reached higher saturation for 500 mg/L of glucose at lower resistances of 100 and 470 ohm, in comparison to 300 mg/L at 1000 ohm. The results show that the response of the MFC can potentially be tuned by adjusting the external load. Our preliminary study suggests that Shewanella baltica strain 20 may be used for online monitoring of BOD (biological oxygen demand) in wastewater. LA - English DB - MTMT ER - TY - JOUR AU - Maroua, Oueslati AU - Bali, Dominika AU - Papp, Szilvia AU - Schneider, György AU - Kovács, Tamás TI - Phenotypic behavior and assessing phage susceptibility in pathogenic Xanthomonas arboricola pv. juglandis JF - ACTA HORTICULTURAE: TECHNICAL COMMUNICATIONS OF ISHS J2 - ACTA HORTICULTURAE PY - 2024 PG - 15 SN - 0567-7572 UR - https://m2.mtmt.hu/api/publication/34720693 ID - 34720693 LA - English DB - MTMT ER - TY - JOUR AU - Schneider, György AU - Seres-Steinbach, Anita AU - Putics, Ákos AU - Solti-Hodován, Ágnes AU - Palkovics, Tamás TI - Potential of Essential Oils in the Control of Listeria monocytogenes JF - MICROORGANISMS J2 - MICROORGANISMS VL - 11 PY - 2023 IS - 6 PG - 35 SN - 2076-2607 DO - 10.3390/microorganisms11061364 UR - https://m2.mtmt.hu/api/publication/34050794 ID - 34050794 N1 - Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti St. 12, Pécs, H-7624, Hungary Central Laboratory, Aladár Petz Teaching Hospital, Vasvári Pál Street 2-4, Győr, H-9024, Hungary Cited By :1 Export Date: 6 September 2023 Correspondence Address: Schneider, G.; Department of Medical Microbiology and Immunology, Szigeti St. 12, Hungary; email: schneider.gyorgy@pte.hu AB - Listeria monocytogenes is a foodborne pathogen, the causative agent of listeriosis. Infections typically occur through consumption of foods, such as meats, fisheries, milk, vegetables, and fruits. Today, chemical preservatives are used in foods; however, due to their effects on human health, attention is increasingly turning to natural decontamination practices. One option is the application of essential oils (EOs) with antibacterial features, since EOs are considered by many authorities as being safe. In this review, we aimed to summarize the results of recent research focusing on EOs with antilisterial activity. We review different methods via which the antilisterial effect and the antimicrobial mode of action of EOs or their compounds can be investigated. In the second part of the review, results of those studies from the last 10 years are summarized, in which EOs with antilisterial effects were applied in and on different food matrices. This section only included those studies in which EOs or their pure compounds were tested alone, without combining them with any additional physical or chemical procedure or additive. Tests were performed at different temperatures and, in certain cases, by applying different coating materials. Although certain coatings can enhance the antilisterial effect of an EO, the most effective way is to mix the EO into the food matrix. In conclusion, the application of EOs is justified in the food industry as food preservatives and could help to eliminate this zoonotic bacterium from the food chain. LA - English DB - MTMT ER - TY - JOUR AU - Horváth, Marianna AU - Kovács, Tamás AU - Kun, József AU - Gyenesei, Attila AU - Damjanova, Ivelina AU - Tigyi, Zoltán AU - Schneider, György TI - Virulence Characteristics and Molecular Typing of Carbapenem-Resistant ST15 Klebsiella pneumoniae Clinical Isolates, Possessing the K24 Capsular Type JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 3 PG - 22 SN - 2079-6382 DO - 10.3390/antibiotics12030479 UR - https://m2.mtmt.hu/api/publication/33728546 ID - 33728546 AB - Klebsiella pneumoniae is an opportunistic pathogen that frequently causes nosocomial and community-acquired (CA) infections. Until now, a limited number of studies has been focused on the analyses of changes affecting the virulence attributes. Genotypic and phenotypic methods were used to characterise the 39 clinical K. pneumoniae isolates; all belonged to the pan-drug resistant, widespread clone ST 15 and expressed the K24 capsule. PFGE has revealed that the isolates could be divided into three distinct genomic clusters. All isolates possessed allS and uge genes, known to contribute to the virulence of K. pneumoniae and 10.25% of the isolates showed hypermucoviscosity, 94.87% produced type 1 fimbriae, 92.3% produced type 3 fimbriae, and 92.3% were able to produce biofilm. In vivo persistence could be supported by serum resistance 46.15%, enterobactin (94.87%) and aerobactin (5.12%) production and invasion of the INT407 and T24 cell lines. Sequence analysis of the whole genomes of the four representative strains 11/3, 50/1, 53/2 and 53/3 has revealed high sequence homology to the reference K. pneumoniae strain HS11286. Our results represent the divergence of virulence attributes among the isolates derived from a common ancestor clone ST 15, in an evolutionary process that occurred both in the hospital and in the community. LA - English DB - MTMT ER - TY - JOUR AU - Pertics, Botond Zsombor AU - Kovács, Tamás AU - Schneider, György TI - Characterization of a Lytic Bacteriophage and Demonstration of Its Combined Lytic Effect with a K2 Depolymerase on the Hypervirulent Klebsiella pneumoniae Strain 52145 JF - MICROORGANISMS J2 - MICROORGANISMS VL - 11 PY - 2023 IS - 3 PG - 26 SN - 2076-2607 DO - 10.3390/microorganisms11030669 UR - https://m2.mtmt.hu/api/publication/33728531 ID - 33728531 AB - Klebsiella pneumoniae is a nosocomial pathogen. Among its virulence factors is the capsule with a prominent role in defense and biofilm formation. Bacteriophages (phages) can evoke the lysis of bacterial cells. Due to the mode of action of their polysaccharide depolymerase enzymes, phages are typically specific for one bacterial strain and its capsule type. In this study, we characterized a bacteriophage against the capsule-defective mutant of the nosocomial K. pneumoniae 52145 strain, which lacks K2 capsule. The phage showed a relatively narrow host range but evoked lysis on a few strains with capsular serotypes K33, K21, and K24. Phylogenetic analysis showed that the newly isolated Klebsiella phage 731 belongs to the Webervirus genus in the Drexlerviridae family; it has a 31.084 MDa double-stranded, linear DNA with a length of 50,306 base pairs and a G + C content of 50.9%. Out of the 79 open reading frames (ORFs), we performed the identification of orf22, coding for a trimeric tail fiber protein with putative capsule depolymerase activity, along with the mapping of other putative depolymerases of phage 731 and homologous phages. Efficacy of a previously described recombinant K2 depolymerase (B1dep) was tested by co-spotting phage 731 on K. pneumoniae strains, and it was demonstrated that the B1dep-phage 731 combination allows the lysis of the wild type 52145 strain, originally resistant to the phage 731. With phage 731, we showed that B1dep is a promising candidate for use as a possible antimicrobial agent, as it renders the virulent strain defenseless against other phages. Phage 731 alone is also important due to its efficacy on K. pneumoniae strains possessing epidemiologically important serotypes. LA - English DB - MTMT ER - TY - JOUR AU - Schneider, György AU - Pásztor, Dorina AU - Szabó, Péter AU - Kőrösi, László AU - Kishan, Nandyala Siva AU - Raju, Penmetsa Appala Rama Krishna AU - Calay, Rajnish Kaur TI - Isolation and Characterisation of Electrogenic Bacteria from Mud Samples JF - MICROORGANISMS J2 - MICROORGANISMS VL - 11 PY - 2023 IS - 3 PG - 15 SN - 2076-2607 DO - 10.3390/microorganisms11030781 UR - https://m2.mtmt.hu/api/publication/33728530 ID - 33728530 AB - To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 °C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications. LA - English DB - MTMT ER - TY - JOUR AU - Schweitzer, Bettina AU - Balázs, Viktória Lilla AU - Molnár, Szilárd AU - Szögi-Tatár, Bernadett AU - Böszörményi, Andrea AU - Palkovics, Tamás AU - Horváth, Györgyi AU - Schneider, György TI - Antibacterial Effect of Lemongrass (Cymbopogon citratus) against the Aetiological Agents of Pitted Keratolyis JF - MOLECULES J2 - MOLECULES VL - 27 PY - 2022 IS - 4 PG - 14 SN - 1420-3049 DO - 10.3390/molecules27041423 UR - https://m2.mtmt.hu/api/publication/32699034 ID - 32699034 LA - English DB - MTMT ER - TY - JOUR AU - Koderi Valappil, Sarshad AU - Shetty, Prateek AU - Deim, Zoltán AU - Terhes, Gabriella AU - Zsoldiné Urbán, Edit AU - Váczi, Sándor AU - Patai, Roland AU - Polgár, Tamás Ferenc AU - Pertics, Botond Zsombor AU - Schneider, György AU - Kovács, Tamás AU - Rákhely, Gábor TI - Survival Comes at a Cost: A Coevolution of Phage and Its Host Leads to Phage Resistance and Antibiotic Sensitivity of Pseudomonas aeruginosa Multidrug Resistant Strains JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 12 PY - 2021 PG - 17 SN - 1664-302X DO - 10.3389/fmicb.2021.783722 UR - https://m2.mtmt.hu/api/publication/32548543 ID - 32548543 AB - The increasing ineffectiveness of traditional antibiotics and the rise of multidrug resistant (MDR) bacteria have necessitated the revival of bacteriophage (phage) therapy. However, bacteria might also evolve resistance against phages. Phages and their bacterial hosts coexist in nature, resulting in a continuous coevolutionary competition for survival. We have isolated several clinical strains of Pseudomonas aeruginosa and phages that infect them. Among these, the PIAS (Phage Induced Antibiotic Sensitivity) phage belonging to the Myoviridae family can induce multistep genomic deletion in drug-resistant clinical strains of P. aeruginosa, producing a compromised drug efflux system in the bacterial host. We identified two types of mutant lines in the process: green mutants with SNPs (single nucleotide polymorphisms) and smaller deletions and brown mutants with large (∼250 kbp) genomic deletion. We demonstrated that PIAS used the MexXY-OprM system to initiate the infection. P. aeruginosa clogged PIAS phage infection by either modifying or deleting these receptors. The green mutant gaining phage resistance by SNPs could be overcome by evolved PIASs (E-PIASs) with a mutation in its tail-fiber protein. Characterization of the mutant phages will provide a deeper understanding of phage-host interaction. The coevolutionary process continued with large deletions in the same regions of the bacterial genomes to block the (E-)PIAS infection. These mutants gained phage resistance via either complete loss or substantial modifications of the phage receptor, MexXY-OprM, negating its essential role in antibiotic resistance. In vitro and in vivo studies indicated that combined use of PIAS and antibiotics could effectively inhibit P. aeruginosa growth. The phage can either eradicate bacteria or induce antibiotic sensitivity in MDR-resistant clinical strains. We have explored the potential use of combination therapy as an alternative approach against MDR P. aeruginosa infection. LA - English DB - MTMT ER - TY - CHAP AU - Böszörményi, Andrea AU - Szögi-Tatár, Bernadett AU - Horváth, Adrienn AU - Balázs, Viktória Lilla AU - Kovács, Judit Klára AU - Schneider, György AU - Horváth, Györgyi TI - 100%-ban tiszta és természetes: Mi rejlik egy illóolajos üvegben? T2 - METT25 a Magyar Elválasztástudományi Társaság jubileumi konferenciája PB - Magyar Elválasztástudományi Társaság CY - Pécs SN - 9786155270666 PY - 2021 UR - https://m2.mtmt.hu/api/publication/32474386 ID - 32474386 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Naorem, Romen Singh AU - Goswami, Gunajit AU - Schneider, György AU - Fekete, Csaba TI - Comparative analysis of prophages carried by human and animal-associated Staphylococcus aureus strains spreading across the European regions JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-021-98432-8 UR - https://m2.mtmt.hu/api/publication/32236655 ID - 32236655 LA - English DB - MTMT ER -