TY - JOUR AU - Slézia, Andrea AU - Hegedüs, Panna AU - Rusina, Evgeniia AU - Lengyel, Katalin AU - Solari, Nicola AU - Kaszás, Attila AU - Balázsfi, Diána AU - Botzanowski, Boris AU - Acerbo, Emma AU - Missey, Florian AU - Williamson, Adam AU - Hangya, Balázs TI - Behavioral, neural and ultrastructural alterations in a graded-dose 6-OHDA mouse model of early-stage Parkinson's disease JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 17 SN - 2045-2322 DO - 10.1038/s41598-023-46576-0 UR - https://m2.mtmt.hu/api/publication/34341943 ID - 34341943 AB - Studying animal models furthers our understanding of Parkinson’s disease (PD) pathophysiology by providing tools to investigate detailed molecular, cellular and circuit functions. Different versions of the neurotoxin-based 6-hydroxydopamine (6-OHDA) model of PD have been widely used in rats. However, these models typically assess the result of extensive and definitive dopaminergic lesions that reflect a late stage of PD, leading to a paucity of studies and a consequential gap of knowledge regarding initial stages, in which early interventions would be possible. Additionally, the better availability of genetic tools increasingly shifts the focus of research from rats to mice, but few mouse PD models are available yet. To address these, we characterize here the behavioral, neuronal and ultrastructural features of a graded-dose unilateral, single-injection, striatal 6-OHDA model in mice, focusing on early-stage changes within the first two weeks of lesion induction. We observed early onset, dose-dependent impairments of overall locomotion without substantial deterioration of motor coordination. In accordance, histological evaluation demonstrated a partial, dose-dependent loss of dopaminergic neurons of substantia nigra pars compacta (SNc). Furthermore, electron microscopic analysis revealed degenerative ultrastructural changes in SNc dopaminergic neurons. Our results show that mild ultrastructural and cellular degradation of dopaminergic neurons of the SNc can lead to certain motor deficits shortly after unilateral striatal lesions, suggesting that a unilateral dose-dependent intrastriatal 6-OHDA lesion protocol can serve as a successful model of the early stages of Parkinson’s disease in mice. LA - English DB - MTMT ER - TY - JOUR AU - Fekete, Zoltán AU - Zátonyi, Anita AU - Kaszás, Attila AU - Madarász, Miklós AU - Slézia, Andrea TI - Transparent neural interfaces: challenges and solutions of microengineered multimodal implants designed to measure intact neuronal populations using high-resolution electrophysiology and microscopy simultaneously JF - MICROSYSTEMS & NANOENGINEERING J2 - MICROSYST NANOENG VL - 9 PY - 2023 PG - 30 SN - 2055-7434 DO - 10.1038/s41378-023-00519-x UR - https://m2.mtmt.hu/api/publication/33729235 ID - 33729235 N1 - Research Group for Implantable Microsystems, Faculty of Information Technology & Bionics, Pázmány Péter Catholic University, Budapest, Hungary Institute of Cognitive Neuroscience & Psychology, Eotvos Lorand Research Network, Budapest, Hungary Mines Saint-Etienne, Centre CMP, Département BEL, Gardanne, F - 13541, France Institut de Neurosciences de la Timone, CNRS UMR 7289 & Aix-Marseille Université, Marseille, 13005, France János Szentágothai PhD Program of Semmelweis University, Budapest, Hungary BrainVision Center, Budapest, Hungary Cited By :1 Export Date: 1 February 2024 Correspondence Address: Fekete, Z.; Research Group for Implantable Microsystems, Hungary; email: fekete.zoltan@itk.ppke.hu Funding details: NAP2022-I-8/2022 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, FK 134403, TKP2021-EGA-42 Funding details: National Research, Development and Innovation Office Funding text 1: The authors are grateful for the funding of the National Development and Innovation Office (NKFIH FK 134403 and TKP2021-EGA-42 to Z.F.) and the support of the Hungarian Brain Research Program (NAP2022-I-8/2022). LA - English DB - MTMT ER - TY - JOUR AU - Kaszas, A. AU - Szalay, Gergely AU - Slézia, Andrea AU - Bojdán, Alexandra AU - Vanzetta, I. AU - Hangya, Balázs AU - Rózsa J., Balázs AU - O’Connor, R. AU - Moreau, D. TI - Two-photon GCaMP6f imaging of infrared neural stimulation evoked calcium signals in mouse cortical neurons in vivo JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-021-89163-x UR - https://m2.mtmt.hu/api/publication/32030456 ID - 32030456 N1 - Mines Saint-Etienne, Centre CMP, Département BEL, Gardanne, F - 13541, France Institut de Neurosciences de la Timone, CNRS UMR 7289 & Aix-Marseille Université, Marseille, 13005, France Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Budapest, 1083, Hungary Lendület Laboratory of Systems Neuroscience, Institute of Experimental Medicine, Budapest, 1083, Hungary Two-Photon Laboratory, Faculty of Information Technology, Pázmány Péter Catholic University, Budapest, 1083, Hungary Cited By :16 Export Date: 12 February 2024 Correspondence Address: Moreau, D.; Mines Saint-Etienne, France; email: david.moreau@emse.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; Calcium Funding details: Aix-Marseille Université, AMU Funding details: Horizon 2020 Framework Programme, H2020, 715043 Funding details: European Research Council, ERC, 682426 Funding details: Agence Nationale de la Recherche, ANR Funding text 1: Disclosures. AK and IV were funded by ANR [TRAJECTORY] and recurrent funding from CNRS & Aix Marseille Université. RO and DM were funded by foundation EDF ATPulseGliome. GS AB and BR were funded by ERC 682426, VISONby3DSTIM. AS and BH were funded by ERC StG 715043. The authors are grateful to K. Lengyel for technical assistance. We acknowledge the help of the Nikon Microscopy Center at the Institute of Experimental Medicine, Budapest, Hungary. All experimental protocols were approved by the Animal Care and Experimentation Committee of the Institute of Experimental Medicine of the Hungarian Academy of Sciences (approval reference numbers PEI/001/194-4/2014 and PEI/001/1771-2/2015) and by the French Ministry of Higher Education, Research and Innovation (approval reference number APAFIS#22182–2019091818381928 v5). All procedures complied with Hungarian, French and European regulations for animal research. LA - English DB - MTMT ER - TY - JOUR AU - Vicente, A.F. AU - Slézia, Andrea AU - Ghestem, A. AU - Bernard, C. AU - Quilichini, P.P. TI - In Vivo Characterization of Neurophysiological Diversity in the Lateral Supramammillary Nucleus during Hippocampal Sharp-wave Ripples of Adult Rats JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 435 PY - 2020 SP - 95 EP - 111 PG - 17 SN - 0306-4522 DO - 10.1016/j.neuroscience.2020.03.034 UR - https://m2.mtmt.hu/api/publication/31310201 ID - 31310201 N1 - Export Date: 6 April 2021 CODEN: NRSCD Correspondence Address: Vicente, A.F.; Aix Marseille Univ, France; email: ana-maria.fernandez-vicente@univ-amu.fr Funding details: FP7/20072013, no602102 Funding details: Fondation pour la Recherche Médicale, FRM Funding text 1: This work was supported by the European Union’s Seventh Framework Program [ FP7/20072013 ] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Funding text 2: This work was supported by the European Union's Seventh Framework Program [FP7/20072013] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Export Date: 14 April 2021 CODEN: NRSCD Correspondence Address: Vicente, A.F.; Aix Marseille Univ, France; email: ana-maria.fernandez-vicente@univ-amu.fr Funding details: FP7/20072013, no602102 Funding details: Fondation pour la Recherche Médicale, FRM Funding text 1: This work was supported by the European Union’s Seventh Framework Program [ FP7/20072013 ] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Funding text 2: This work was supported by the European Union's Seventh Framework Program [FP7/20072013] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Export Date: 20 April 2021 CODEN: NRSCD Correspondence Address: Vicente, A.F.; Aix Marseille Univ, France; email: ana-maria.fernandez-vicente@univ-amu.fr Funding details: FP7/20072013, no602102 Funding details: Fondation pour la Recherche Médicale, FRM Funding text 1: This work was supported by the European Union’s Seventh Framework Program [ FP7/20072013 ] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Funding text 2: This work was supported by the European Union's Seventh Framework Program [FP7/20072013] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Export Date: 22 April 2021 CODEN: NRSCD Correspondence Address: Vicente, A.F.; Aix Marseille Univ, France; email: ana-maria.fernandez-vicente@univ-amu.fr Funding details: FP7/20072013, no602102 Funding details: Fondation pour la Recherche Médicale, FRM Funding text 1: This work was supported by the European Union’s Seventh Framework Program [ FP7/20072013 ] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. Funding text 2: This work was supported by the European Union's Seventh Framework Program [FP7/20072013] under grant agreement no602102 (EPITARGET). PPQ wishes to thank support from FRM, FFRE, and CURE Epilepsy Taking Flight Award. LA - English DB - MTMT ER - TY - JOUR AU - Slézia, Andrea AU - Proctor, Christopher M. AU - Kaszas, Attila AU - Malliaras, George G. AU - Williamson, Adam (John) TI - Electrophoretic Delivery of gamma-aminobutyric Acid (GABA) into Epileptic Focus Prevents Seizures in Mice JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2019 IS - 147 PG - 9 SN - 1940-087X DO - 10.3791/59268 UR - https://m2.mtmt.hu/api/publication/30792625 ID - 30792625 N1 - Cited By :3 Export Date: 16 May 2023 Correspondence Address: Williamson, A.; Aix Marseille Université, France; email: adam.williamson@univ-amu.fr Chemicals/CAS: 4 aminobutyric acid, 28805-76-7, 56-12-2; fampridine, 1003-40-3, 504-24-5; 4-Aminopyridine; gamma-Aminobutyric Acid Funding details: Institute of International Education, IIE Funding details: Whitaker International Fellows and Scholars Program Funding details: Horizon 2020 Framework Programme, H2020, 625372, 716867 Funding details: Marie Curie Funding details: European Research Council, ERC Funding details: Université Pierre et Marie Curie, UPMC Funding text 1: C.M.P. acknowledges funding from a Whitaker International Scholar grant administered by the Institute for International Education. A.K. was sponsored by the Marie Curie IEF (No. 625372). A.W. acknowledges funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No. 716867). A.W. additionally acknowledges the Excellence Initiative of Aix-Marseille University - A*MIDEX, a French “Investissements d’Avenir” programme. The authors acknowledge Dr. Ilke Uguz, Dr. Sahika Inal, Dr. Vincenzo Curto, Dr. Mary Donahue, Dr. Marc Ferro, and Zsófia Maglóczky for their participation in fruitful discussions. Funding text 2: C.M.P. acknowledges funding from a Whitaker International Scholar grant administered by the Institute for International Education. A.K. was sponsored by the Marie Curie IEF (No. 625372). A.W. acknowledges funding from the European Research Council (ERC) under the European Union?s Horizon 2020 research and innovation programme (grant agreement No. 716867). A.W. additionally acknowledges the Excellence Initiative of Aix-Marseille University - A*MIDEX, a French ?Investissements d?Avenir? programme. The authors acknowledge Dr. Ilke Uguz, Dr. Sahika Inal, Dr. Vincenzo Curto, Dr. Mary Donahue, Dr. Marc Ferro, and Zs?fia Magl?czky for their participation in fruitful discussions. AB - Epilepsy is a group of neurological disorders which affects millions of people worldwide. Although treatment with medication is helpful in 70% of the cases, serious side effects affect the quality of life of patients. Moreover, a high percentage of epileptic patients are drug resistant; in their case, neurosurgery or neurostimulation are necessary. Therefore, the major goal of epilepsy research is to discover new therapies which are either capable of curing epilepsy without side effects or preventing recurrent seizures in drug-resistant patients. Neuroengineering provides new approaches by using novel strategies and technologies to find better solutions to cure epileptic patients at risk.As a demonstration of a novel experimental protocol in an acute mouse model of epilepsy, a direct in situ electrophoretic drug delivery system is used. Namely, a neural probe incorporating a microfluidic ion pump (mu FIP) for on-demand drug delivery and simultaneous recording of local neural activity is implanted and demonstrated to be capable of controlling 4-aminopyridine-induced (4AP-induced) seizure-like event (SLE) activity. The gamma-aminobutyric acid (GABA) concentration is kept in the physiological range by the precise control of GABA delivery to reach an antiepileptic effect in the seizure focus but not to cause overinhibition-induced rebound bursts. The method allows both the detection of pathological activity and intervention to stop seizures by delivering inhibitory neurotransmitters directly to the epileptic focus with precise spatiotemporal control.As a result of the developments to the experimental method, SLEs can be induced in a highly localized manner that allows seizure control by the precisely tuned GABA delivery at the seizure onset. LA - English DB - MTMT ER - TY - JOUR AU - Proctor, Christopher M. AU - Uguz, Ilke AU - Slézia, Andrea AU - Curto, Vincenzo AU - Inal, Sahika AU - Williamson, Adam (John) AU - Malliaras, George G. TI - An Electrocorticography Device with an Integrated Microfluidic Ion Pump for Simultaneous Neural Recording and Electrophoretic Drug Delivery In Vivo JF - ADVANCED BIOSYSTEMS J2 - ADV BIOSYST VL - 3 PY - 2019 IS - 2 PG - 6 SN - 2366-7478 DO - 10.1002/adbi.201800270 UR - https://m2.mtmt.hu/api/publication/30436345 ID - 30436345 AB - The challenge of treating neurological disorders has motivated the development of implantable devices that can deliver treatment when and where it is needed. This study presents a novel brain implant capable of electrophoretically delivering drugs and recording local neural activity on the surface of the brain. The drug delivery is made possible by the integration of a microfluidic ion pump (mu FIP) into a conformable electrocorticography (ECoG) device with recording cites embedded next to the drug delivery outlets. The mu FIP ECoG device can deliver a high capacity of several biologically important cationic species on demand. The therapeutic potential of the device is demonstrated by using it to deliver neurotransmitters in a rodent model while simultaneously recording local neural activity. These developments represent a significant step forward for cortical drug-delivery systems. LA - English DB - MTMT ER - TY - GEN AU - Marc, D. Ferro AU - Christopher, M. Proctor AU - Alexander, Gonzalez AU - Eric, Zhao AU - Slézia, Andrea AU - Jolien, Pas AU - Gerwin, Dijk AU - Mary, J. Donahue AU - Adam, Williamson AU - Georges, G. Malliaras AU - Lisa, Giocomo AU - Nicholas, A. Melosh TI - NeuroRoots, a bio-inspired, seamless Brain Machine Interface device for long-term recording PY - 2018 DO - 10.1101/460949 UR - https://m2.mtmt.hu/api/publication/32561425 ID - 32561425 LA - English DB - MTMT ER - TY - JOUR AU - Pas, Jolien AU - Rutz, Alexandra L. AU - Quilichini, Pascale P. AU - Slézia, Andrea AU - Ghestem, Antoine AU - Kaszas, Attila AU - Donahue, Mary J. AU - Curto, Vincenzo F. AU - O'Connor, Rodney P. AU - Bernard, Christophe AU - Williamson, Adam AU - Malliaras, George G. TI - A bilayered PVA/PLGA-bioresorbable shuttle to improve the implantation of flexible neural probes JF - JOURNAL OF NEURAL ENGINEERING J2 - J NEURAL ENG VL - 15 PY - 2018 IS - 6 PG - 10 SN - 1741-2560 DO - 10.1088/1741-2552/aadc1d UR - https://m2.mtmt.hu/api/publication/30580269 ID - 30580269 N1 - Department of Bioelectronics, Mines Saint-Etienne, Center of Microelectronics in Provence, Gardanne, F-13541, France Panaxium SAS, 67 Cours Mirabeau, Aix-en-Provence, 13100, France Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, 13005, France Electrical Engineering Division, Department of Engineering, University of Cambridge, 9 JJ Thomson Ave, Cambridge, CB3 0FA, United Kingdom Aix-Marseille Univ, CNRS UMR 7289, Inst de Neurosci de la Timone, Marseille, 13005, France Cited By :13 Export Date: 17 March 2021 Correspondence Address: Pas, J.; Department of Bioelectronics, Center of Microelectronics in Provence, F-13541 Gardanne, France Chemicals/CAS: acetone, 67-64-1; agarose, 9012-36-6; polyglactin, 26780-50-7, 34346-01-5; polyvinyl alcohol, 37380-95-3, 9002-89-5; tenidap, 100599-27-7, 120210-48-2; Biocompatible Materials; Polylactic Acid-Polyglycolic Acid Copolymer; Polyvinyl Alcohol Funding details: Horizon 2020 Framework Programme, H2020, 716867, 732032 Department of Bioelectronics, Mines Saint-Etienne, Center of Microelectronics in Provence, Gardanne, F-13541, France Panaxium SAS, 67 Cours Mirabeau, Aix-en-Provence, 13100, France Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, 13005, France Electrical Engineering Division, Department of Engineering, University of Cambridge, 9 JJ Thomson Ave, Cambridge, CB3 0FA, United Kingdom Aix-Marseille Univ, CNRS UMR 7289, Inst de Neurosci de la Timone, Marseille, 13005, France Cited By :14 Export Date: 20 April 2021 Correspondence Address: Pas, J.; Department of Bioelectronics, Center of Microelectronics in Provence, F-13541 Gardanne, France Chemicals/CAS: acetone, 67-64-1; agarose, 9012-36-6; polyglactin, 26780-50-7, 34346-01-5; polyvinyl alcohol, 37380-95-3, 9002-89-5; tenidap, 100599-27-7, 120210-48-2; Biocompatible Materials; Polylactic Acid-Polyglycolic Acid Copolymer; Polyvinyl Alcohol Funding details: Horizon 2020 Framework Programme, H2020, 716867, 732032 AB - Objective. Neural electrophysiology is often conducted with traditional, rigid depth probes. The mechanical mismatch between these probes and soft brain tissue is unfavorable for tissue interfacing. Making probes compliant can improve biocompatibility, but as a consequence, they become more difficult to insert into the brain. Therefore, new methods for inserting compliant neural probes must be developed. Approach. Here, we present a new bioresorbable shuttle based on the hydrolytically degradable poly(vinyl alcohol) (PVA) and poly(lactic-coglycolic acid) (PLGA). We show how to fabricate the PVA/PLGA shuttles on flexible and thin parylene probes. The method consists of PDMS molding used to fabricate a PVA shuttle aligned with the probe and to also impart a sharp tip necessary for piercing brain tissue. The PVA shuttle is then dip-coated with PLGA to create a bi-layered shuttle. Main results. While single layered PVA shuttles are able to penetrate agarose brain models, only limited depths were achieved and repositioning was not possible due to the fast degradation. We demonstrate that a bilayered approach incorporating a slower dissolving PLGA layer prolongs degradation and enables facile insertion for at least several millimeters depth. Impedances of electrodes before and after shuttle preparation were characterized and showed that careful deposition of PLGA is required to maintain low impedance. Facilitated by the shuttles. compliant parylene probes were also successfully implanted into anaesthetized mice and enabled the recording of high quality local field potentials. Significance. This work thereby presents a solution towards addressing a key challenge of implanting compliant neural probes using a two polymer system. PVA and PLGA are polymers with properties ideal for translation: commercially available, biocompatible with FDA-approved uses and bioresorbable. By presenting new ways to implant compliant neural probes, we can begin to fully evaluate their chronic biocompatibility and performance compared to traditional, rigid electronics. LA - English DB - MTMT ER - TY - JOUR AU - Proctor, Christopher M. AU - Slézia, Andrea AU - Kaszas, Attila AU - Ghestem, Antoine AU - del Agua, Isabel AU - Pappa, Anna-Maria AU - Bernard, Christophe AU - Williamson, Adam AU - Malliaras, George G. TI - Electrophoretic drug delivery for seizure control JF - SCIENCE ADVANCES J2 - SCI ADV VL - 4 PY - 2018 IS - 8 PG - 8 SN - 2375-2548 DO - 10.1126/sciadv.aau1291 UR - https://m2.mtmt.hu/api/publication/30484866 ID - 30484866 N1 - CHRISTOPHER M. PROCTOR and ANDREA SLÉZIA contributed equally to this work. AB - The persistence of intractable neurological disorders necessitates novel therapeutic solutions. We demonstrate the utility of direct in situ electrophoretic drug delivery to treat neurological disorders. We present a neural probe incorporating a microfluidic ion pump (mu FIP) for on-demand drug delivery and electrodes for recording local neural activity. The mu FIP works by electrophoretically pumping ions across an ion exchange membrane and thereby delivers only the drug of interest and not the solvent. This "dry" delivery enables precise drug release into the brain region with negligible local pressure increase. The therapeutic potential of the mu FIP probe is tested in a rodent model of epilepsy. The mu FIP probe can detect pathological activity and then intervene to stop seizures by delivering inhibitory neurotransmitters directly to the seizure source. We anticipate that further tailored engineering of the mu FIP platform will enable additional applications in neural interfacing and the treatment of neurological disorders. LA - English DB - MTMT ER - TY - JOUR AU - Donahue, Mary J. AU - Kaszas, Attila AU - Turi, Gergely F. AU - Rózsa J., Balázs AU - Slézia, Andrea AU - Vanzetta, Ivo AU - Katona, Gergely AU - Bernard, Christophe AU - Malliaras, George G. AU - Williamson, Adam (John) TI - Multimodal Characterization of Neural Networks using Highly Transparent Electrode Arrays JF - ENEURO J2 - ENEURO VL - 5 PY - 2018 IS - 6 SN - 2373-2822 DO - 10.1523/ENEURO.0187-18.2018 UR - https://m2.mtmt.hu/api/publication/30382220 ID - 30382220 N1 - Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 30 July 2019 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Funding details: University of Cambridge Funding details: Ecole Supérieure des Communications de Tunis Funding details: Institut National de la Santé et de la Recherche Médicale, Inserm Funding details: Centre National de la Recherche Scientifique, CNRS Funding details: Marie Curie, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding details: 716867 Funding text 1: 1Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne 13541, France, 2Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France, 3Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, 4Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest H-1083, Hungary, 5Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France, 6Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged 6720, Hungary, 7Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest H-1083, Hungary, and 8Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge CB3 0FA, United Kingdom Funding text 2: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 15 February 2020 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: University of Cambridge Funding details: Ecole Supérieure des Communications de Tunis Funding details: Institut National de la Santé et de la Recherche Médicale, Inserm Funding details: Centre National de la Recherche Scientifique, CNRS Funding details: Marie Curie, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding details: 716867 Funding text 1: 1Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne 13541, France, 2Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France, 3Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, 4Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest H-1083, Hungary, 5Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France, 6Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged 6720, Hungary, 7Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest H-1083, Hungary, and 8Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge CB3 0FA, United Kingdom Funding text 2: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 17 April 2020 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Université Pierre et Marie Curie, UPMC, 625372 Funding details: European Research Council, ERC Funding details: Horizon 2020 Framework Programme, H2020, 716867 Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Export Date: 22 April 2020 Correspondence Address: Williamson, A.; Aix- Marseille Université, Inserm U1106 INS, Institut de Neurosciences des SystèmesFrance; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Université Pierre et Marie Curie, UPMC, 625372 Funding details: European Research Council, ERC Funding details: Horizon 2020 Framework Programme, H2020, 716867 Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, KFI-2016-0177, GINOP-2016-00979, NVKP-2016-0043, ERC682426 Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Cited By :1 Export Date: 17 March 2021 Correspondence Address: Williamson, A.; Aix- Marseille Université, France; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Horizon 2020 Framework Programme, H2020, 682426, 716867 Funding details: Seventh Framework Programme, FP7, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, ERC682426, GINOP-2016-00979, KFI-2016-0177, NVKP-2016-0043 Funding details: Université Pierre et Marie Curie, UPMC Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. Department of Bioelectronics, Ecole Nationale Supérieure des Mines, Centre of Microelectronics in Provence, Gardanne, 13541, France Aix Marseille Univ, CNRS, INT, Inst Neurosci Timone, Marseille, France Department of Psychiatry, Division of Systems Neuroscience, Columbia University and Research Foundation for Mental Hygiene, New York State Psychiatric Institute, New York, NY 10032, United States Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, H-1083, Hungary Aix Marseille Univ, INSERM, INS, Inst Neurosci Syst, Marseille, France Neuroengineering Research Group, Interdisciplinary Excellence Center, Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, 6720, Hungary Two-Photon Measurement Technology Research Group, Pázmány Péter Catholic University, Budapest, H-1083, Hungary Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA, United Kingdom Cited By :1 Export Date: 20 April 2021 Correspondence Address: Williamson, A.; Aix- Marseille Université, France; email: adam.williamson@univ-amu.fr Chemicals/CAS: calcium, 7440-70-2, 14092-94-5; fampridine, 1003-40-3, 504-24-5 Tradenames: Clevios, Heraeus, Germany Manufacturers: Femtonics; Heraeus, Germany; Scientifica Funding details: Horizon 2020 Framework Programme, H2020, 682426, 716867 Funding details: Seventh Framework Programme, FP7, 625372 Funding details: European Research Council, ERC Funding details: Fondation pour la Recherche Médicale, FRM, DBS20131128446, ERC682426, GINOP-2016-00979, KFI-2016-0177, NVKP-2016-0043 Funding text 1: This work was supported by the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme (Grant 716867). A.K. was supported by EC Marie Curie Intra-European Fellowship (ImagINE, Grant 625372). M.J.D. was supported by the Fondation pour la Recherche Médicale Grant DBS20131128446. B.R. and K.G. were supported by ERC682426, KFI-2016-0177, GINOP-2016-00979, and NVKP-2016-0043. *M.J.D. and A.K. contributed equally to this work. AB - Transparent and flexible materials are attractive for a wide range of emerging bioelectronic applications. These include neural interfacing devices for both recording and stimulation, where low electrochemical electrode impedance is valuable. Here the conducting polymer poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS) is utilized to fabricate electrodes that are small enough to allow unencumbered optical access for imaging a large cell population with two-photon (2P) microscopy, yet provide low impedance for simultaneous high quality recordings of neural activity in vivo. To demonstrate this, pathophysiological activity was induced in the mouse cortex using 4-aminopyridine (4AP) and the resulting electrical activity was detected with the PEDOT:PSS-based probe while imaging calcium activity directly below the probe area. The induced calcium activity of the neuronal network as measured by the fluorescence change in the cells correlated well with the electrophysiological recordings from the cortical grid of PEDOT:PSS microelectrodes. Our approach provides a valuable vehicle for complementing classical high temporal resolution electrophysiological analysis with optical imaging. LA - English DB - MTMT ER -