@article{MTMT:34797924,
	title = {Transient Receptor Potential Ankyrin 1 Ion Channel Is Expressed in Osteosarcoma and Its Activation Reduces Viability},
	url = {https://m2.mtmt.hu/api/publication/34797924},
	author = {Hudhud, Lina and Kovács-Rozmer, Katalin and Kecskés, Angéla and Pohóczky, Krisztina and Bencze, Noémi and Buzás, Krisztina and Szőke, Éva and Helyes, Zsuzsanna},
	doi = {10.3390/ijms25073760},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34797924},
	issn = {1661-6596},
	abstract = {Osteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR gel electrophoresis and RNAscope in situ hybridization. The function of these channels was confirmed by their radioactive 45Ca2+ uptake in response to the TRPA1 agonist, Allyl-isothiocyanate (AITC), and TRPV1 agonist, capsaicin, in K7M2 cells. An ATP-based K2M7 cell viability luminescence assay was used to determine cell viability after AITC or capsaicin treatments. Both TRPA1/Trpa1 and TRPV1/Trpv1 were expressed similarly in human and mouse osteosarcoma tissues, while Trpa1 transcripts were more abundantly present in K7M2 cells. TRPA1 activation with 200 µM AITC induced a significant 45Ca2+ influx into K7M2 cells, and the antagonist attenuated this effect. In accordance with the lower Trpv1 expression, capsaicin induced a moderate 45Ca2+ uptake, which did not reach the level of statistical significance. Both AITC and capsaicin significantly reduced K7M2 cell viability, demonstrating EC50 values of 22 µM and 74 µM. The viability-decreasing effect of AITC was significantly but only partially antagonized by HC-030031, but the action of capsaicin was not affected by the TRPV1 antagonist capsazepine. We provide here the first data on the functional expression of the TRPA1 and TRPV1 ion channels in osteosarcoma, suggesting novel diagnostic and/or therapeutic perspectives.},
	keywords = {CAPSAICIN; TRPV1; Cell viability; mustard oil; TRPA1; Osteosarcoma; RNAscope in situ hybridization; radioactive 45Ca2+ uptake},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Pohóczky, Krisztina/0000-0003-0385-5162; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:34567532,
	title = {Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches},
	url = {https://m2.mtmt.hu/api/publication/34567532},
	author = {Welsh, Joshua A. and Goberdhan, Deborah C. I. and O'Driscoll, Lorraine and Buzás, Edit Irén and Blenkiron, Cherie and Bussolati, Benedetta and Cai, Houjian and Di Vizio, Dolores and Driedonks, Tom A. P. and Erdbrügger, Uta and Falcon‐Perez, Juan M. and Fu, Qing‐Ling and Hill, Andrew F. and Lenassi, Metka and Lim, Sai Kiang and Mahoney, Mỹ G. and Mohanty, Sujata and Möller, Andreas and Nieuwland, Rienk and Ochiya, Takahiro and Sahoo, Susmita and Torrecilhas, Ana C. and Zheng, Lei and Zijlstra, Andries and Abuelreich, Sarah and Bagabas, Reem and Bergese, Paolo and Bridges, Esther M. and Brucale, Marco and Burger, Dylan and Carney, Randy P. and Cocucci, Emanuele and Colombo, Federico and Crescitelli, Rossella and Hanser, Edveena and Harris, Adrian L. and Haughey, Norman J. and Hendrix, An and Ivanov, Alexander R. and Jovanovic‐Talisman, Tijana and Kruh‐Garcia, Nicole A. and Ku'ulei‐Lyn Faustino, Vroniqa and Kyburz, Diego and Lässer, Cecilia and Lennon, Kathleen M. and Lötvall, Jan and Maddox, Adam L. and Martens‐Uzunova, Elena S. and Mizenko, Rachel R. and Newman, Lauren A. and Ridolfi, Andrea and Rohde, Eva and Rojalin, Tatu and Rowland, Andrew and Saftics, Andras and Sandau, Ursula S. and Saugstad, Julie A. and Shekari, Faezeh and Swift, Simon and Ter‐Ovanesyan, Dmitry and Tosar, Juan P. and Useckaite, Zivile and Valle, Francesco and Varga, Zoltán and van der Pol, Edwin and van Herwijnen, Martijn J. C. and Wauben, Marca H. M. and Wehman, Ann M. and Williams, Sarah and Zendrini, Andrea and Zimmerman, Alan J. and Théry, Clotilde and Witwer, Kenneth W. and Haseeb, Zubair},
	doi = {10.1002/jev2.12404},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {13},
	unique-id = {34567532},
	abstract = {Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.},
	year = {2024},
	eissn = {2001-3078},
	orcid-numbers = {Welsh, Joshua A./0000-0002-1097-9756; Goberdhan, Deborah C. I./0000-0003-0645-6714; Buzás, Edit Irén/0000-0002-3744-206X; Bussolati, Benedetta/0000-0002-3663-5134; Cai, Houjian/0000-0003-4887-2652; Falcon‐Perez, Juan M./0000-0003-3133-0670; Hill, Andrew F./0000-0001-5581-2354; Lenassi, Metka/0000-0002-9488-6855; Mohanty, Sujata/0000-0002-0047-4914; Nieuwland, Rienk/0000-0002-5671-3400; Ochiya, Takahiro/0000-0002-0776-9918; Sahoo, Susmita/0000-0002-7279-1564; Torrecilhas, Ana C./0000-0001-5724-2199; Zheng, Lei/0000-0003-2576-8780; Zijlstra, Andries/0000-0001-8460-8803; Brucale, Marco/0000-0001-7244-4389; Carney, Randy P./0000-0001-8193-1664; Crescitelli, Rossella/0000-0002-1714-3169; Haughey, Norman J./0000-0001-5194-4122; Martens‐Uzunova, Elena S./0000-0002-5363-2525; Newman, Lauren A./0000-0003-3303-1666; Rohde, Eva/0000-0001-8692-886X; Sandau, Ursula S./0000-0002-3646-7089; Saugstad, Julie A./0000-0002-2996-9611; Shekari, Faezeh/0000-0001-6026-5412; Tosar, Juan P./0000-0002-2021-2479; Varga, Zoltán/0000-0002-5741-2669; Wauben, Marca H. M./0000-0003-0360-0311; Wehman, Ann M./0000-0001-9826-4132; Zimmerman, Alan J./0000-0001-6280-4790; Théry, Clotilde/0000-0001-8294-6884; Witwer, Kenneth W./0000-0003-1664-4233; Bodnár, Bernadett Réka/0000-0003-3347-9225; Bukva, Mátyás/0000-0002-5225-0285; Buzás, Edit Irén/0000-0002-3744-206X; Buzás, Krisztina/0000-0001-8933-2033; Dobra, Gabriella/0000-0002-2814-7720; Försönits, András/0000-0002-9298-8890; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Koncz, Anna/0000-0003-2511-2394; Lőrincz, Márton Ákos/0000-0002-2819-5116; Németh, Krisztina/0000-0002-3825-2137; Oláh, Attila/0000-0003-4122-5639; Osteikoetxea, Xabier/0000-0003-3628-0174; Pálóczi, Krisztina/0000-0001-7065-3582; Stepanova, Ganna/0000-0002-8285-2762; Visnovitz, Tamás/0000-0002-7962-5083; Wiener, Zoltán/0000-0001-7056-4926; Harmati, Mária/0000-0002-4875-5723; Hegyesi, Hargita/0000-0002-8800-5169}
}

@article{MTMT:34417027,
	title = {Machine learning-based analysis of cancer cell-derived vesicular proteins revealed significant tumor-specificity and predictive potential of extracellular vesicles for cell invasion and proliferation - A meta-analysis.},
	url = {https://m2.mtmt.hu/api/publication/34417027},
	author = {Bukva, Mátyás and Dobra, Gabriella and Gyukity-Sebestyén, Edina and Böröczky, Timea and Korsós, Marietta Margaréta and David G, Meckes and Horváth, Péter and Buzás, Krisztina and Harmati, Mária},
	doi = {10.1186/s12964-023-01344-5},
	journal-iso = {CELL COMM SIGN},
	journal = {CELL COMMUNICATION AND SIGNALING},
	volume = {21},
	unique-id = {34417027},
	issn = {1478-811X},
	abstract = {Although interest in the role of extracellular vesicles (EV) in oncology is growing, not all potential aspects have been investigated. In this meta-analysis, data regarding (i) the EV proteome and (ii) the invasion and proliferation capacity of the NCI-60 tumor cell lines (60 cell lines from nine different tumor types) were analyzed using machine learning methods.On the basis of the entire proteome or the proteins shared by all EV samples, 60 cell lines were classified into the nine tumor types using multiple logistic regression. Then, utilizing the Least Absolute Shrinkage and Selection Operator, we constructed a discriminative protein panel, upon which the samples were reclassified and pathway analyses were performed. These panels were validated using clinical data (n = 4,665) from Human Protein Atlas.Classification models based on the entire proteome, shared proteins, and discriminative protein panel were able to distinguish the nine tumor types with 49.15%, 69.10%, and 91.68% accuracy, respectively. Invasion and proliferation capacity of the 60 cell lines were predicted with R2 = 0.68 and R2 = 0.62 (p < 0.0001). The results of the Reactome pathway analysis of the discriminative protein panel suggest that the molecular content of EVs might be indicative of tumor-specific biological processes.Integrating in vitro EV proteomic data, cell physiological characteristics, and clinical data of various tumor types illuminates the diagnostic, prognostic, and therapeutic potential of EVs. Video Abstract.},
	keywords = {CLASSIFICATION; PROLIFERATION; INVASION; PREDICTION; machine learning; Extracellular vesicles; NCI-60},
	year = {2023},
	eissn = {1478-811X},
	orcid-numbers = {Bukva, Mátyás/0000-0002-5225-0285; Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Böröczky, Timea/0009-0009-3390-7809; Buzás, Krisztina/0000-0001-8933-2033; Harmati, Mária/0000-0002-4875-5723}
}

@article{MTMT:34231255,
	title = {Extracellular vesicle-mediated intercellular communication in cancer},
	url = {https://m2.mtmt.hu/api/publication/34231255},
	author = {Harmati, Mária and Bukva, Mátyás and Dobra, Gabriella and Gyukity-Sebestyén, Edina and Böröczky, Timea and Szabó, Zoltán and Kónya, Zoltán and Horvath, Peter and Klekner, Almos and Buzás, Krisztina},
	journal-iso = {EUR J IMMUNOL},
	journal = {EUROPEAN JOURNAL OF IMMUNOLOGY},
	volume = {53},
	unique-id = {34231255},
	issn = {0014-2980},
	year = {2023},
	eissn = {1521-4141},
	pages = {39-40},
	orcid-numbers = {Harmati, Mária/0000-0002-4875-5723; Bukva, Mátyás/0000-0002-5225-0285; Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Böröczky, Timea/0009-0009-3390-7809; Szabó, Zoltán/0000-0001-8278-8038; Kónya, Zoltán/0000-0002-9406-8596; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:34107493,
	title = {Image-based and machine learning-guided multiplexed serology test for SARS-CoV-2},
	url = {https://m2.mtmt.hu/api/publication/34107493},
	author = {Pietiäinen, Vilja and Polso, Minttu and Migh, Ede and Guckelsberger, Christian and Harmati, Mária and Diósdi, Ákos and Turunen, Laura and Hassinen, Antti and Potdar, Swapnil and Koponen, Annika and Gyukity-Sebestyén, Edina and Kovács, Ferenc and Kriston, András and Hollandi, Réka and Burián, Katalin and Terhes, Gabriella and Visnyovszki, Ádám and Fodor, Eszter and Lacza, Zsombor and Kantele, Anu and Kolehmainen, Pekka and Kakkola, Laura and Strandin, Tomas and Levanov, Lev and Kallioniemi, Olli and Kemény, Lajos and Julkunen, Ilkka and Vapalahti, Olli and Buzás, Krisztina and Paavolainen, Lassi and Horváth, Péter and Hepojoki, Jussi},
	doi = {10.1016/j.crmeth.2023.100565},
	journal-iso = {CELL REP METH},
	journal = {CELL REPORTS METHODS},
	volume = {3},
	unique-id = {34107493},
	year = {2023},
	eissn = {2667-2375},
	orcid-numbers = {Harmati, Mária/0000-0002-4875-5723; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Burián, Katalin/0000-0003-1300-2374; Terhes, Gabriella/0000-0002-7301-9672; Kemény, Lajos/0000-0002-2119-9501; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:33592853,
	title = {MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles},
	url = {https://m2.mtmt.hu/api/publication/33592853},
	author = {Dobra, Gabriella and Gyukity-Sebestyén, Edina and Bukva, Mátyás and Harmati, Mária and Nagy, Valentina and Szabó, Zoltán and Pankotai, Tibor and Klekner, Álmos and Buzás, Krisztina},
	doi = {10.3390/cancers15030712},
	journal-iso = {CANCERS},
	journal = {CANCERS},
	volume = {15},
	unique-id = {33592853},
	abstract = {Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood–brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles (sEVs)—which can cross the BBB and are stable in body fluids—to characterise tumours with different invasion capacity. From four patient groups (glioblastoma multiforme, brain metastases of lung cancer, meningioma, and lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating and characterising sEVs, their MMP-9 content was measured by ELISA and assessed statistically (correlation, paired t-test, Welch’s test, ANOVA, ROC). We found that the MMP-9 content of sEVs is independent of gender and age, but is affected by surgical intervention, treatment, and recurrence. We found a relation between low MMP-9 level in sEVs (<28 ppm) and improved survival (8-month advantage) of glioblastoma patients, and MMP-9 levels showed a positive correlation with aggressiveness. These findings suggest that vesicular MMP-9 level might be a useful prognostic marker for brain tumours.},
	year = {2023},
	eissn = {2072-6694},
	orcid-numbers = {Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Bukva, Mátyás/0000-0002-5225-0285; Harmati, Mária/0000-0002-4875-5723; Szabó, Zoltán/0000-0001-8278-8038; Pankotai, Tibor/0000-0001-9810-5465; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:33554742,
	title = {Impact of Experimental Conditions on Extracellular Vesicles’ Proteome: A Comparative Study},
	url = {https://m2.mtmt.hu/api/publication/33554742},
	author = {Böröczky, Timea and Dobra, Gabriella and Bukva, Mátyás and Gyukity-Sebestyén, Edina and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Horváth, Péter and Buzás, Krisztina and Harmati, Mária},
	doi = {10.3390/life13010206},
	journal-iso = {LIFE-BASEL},
	journal = {LIFE-BASEL},
	volume = {13},
	unique-id = {33554742},
	abstract = {Extracellular vesicle (EV) research is a rapidly developing field, mainly due to the key role of EVs in intercellular communication and pathophysiological processes. However, the heterogeneity of EVs challenges their exploration and the establishment of gold-standard methods. Here, we aimed to reveal the influence of technical changes on EV biology and the reliability of experimental data. We used B16F1 melanoma cells as a model and applied nanoparticle tracking analysis, mass spectrometry (LC-MS/MS) and pathway enrichment analysis to analyze the quantity, size distribution, proteome and function of their small EVs (sEVs) produced in sEV-depleted fetal bovine serum (FBS)-containing medium or serum-free medium. Additionally, we investigated the effects of minor technical variances on the quality of sEV preparations. We found that storage of the isolates at −80 °C has no adverse effect on LC-MS/MS analysis, and an additional washing step after differential ultracentrifugation has a minor influence on the sEV proteome. In contrast, FBS starvation affects the production and proteome of sEVs; moreover, these vesicles may have a greater impact on protein metabolism, but a smaller impact on cell adhesion and membrane raft assembly, than the control sEVs. As we demonstrated that FBS starvation has a strong influence on sEV biology, applying serum-free conditions might be considered in in vitro sEV studies.},
	year = {2023},
	eissn = {2075-1729},
	orcid-numbers = {Böröczky, Timea/0009-0009-3390-7809; Dobra, Gabriella/0000-0002-2814-7720; Bukva, Mátyás/0000-0002-5225-0285; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Buzás, Krisztina/0000-0001-8933-2033; Harmati, Mária/0000-0002-4875-5723}
}

@misc{MTMT:33644437,
	title = {A vezikuláris cargo hatása a veleszületett immunitás egyes elemeire},
	url = {https://m2.mtmt.hu/api/publication/33644437},
	author = {Buzás, Krisztina},
	unique-id = {33644437},
	year = {2022},
	orcid-numbers = {Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:33610029,
	title = {Raman spectral signatures of plasma-derived extracellular vesicle-enriched isolates may support the diagnosis of different cancerous diseases},
	url = {https://m2.mtmt.hu/api/publication/33610029},
	author = {Matyas, Bukva and Edina, Gyukity-Sebestyen and Timea, Boroczky and Yasmin, Ranjous and Maria, Harmati and Gabriella, Dobra and Adrienn, Jenei and Laszlo, Szivos and Hideghéty, Katalin and Krisztina, Budai and Oláh, Judit Magdolna and Peter, Horvath and Lázár, György ifj and Tamas, Biro and Zoltan, Konya and Pal, Barzo and Almos, Klekner and Buzás, Krisztina},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {11},
	unique-id = {33610029},
	year = {2022},
	eissn = {2001-3078},
	pages = {263-264},
	orcid-numbers = {Hideghéty, Katalin/0000-0001-7080-2365; Lázár, György ifj/0000-0001-7155-2978; Buzás, Krisztina/0000-0001-8933-2033}
}

@article{MTMT:33609933,
	title = {Donor cell-specific aspects of the small extracellular vesicle production},
	url = {https://m2.mtmt.hu/api/publication/33609933},
	author = {Maria, Harmati and Timea, Boroczky and Matyas, Bukva and Gabriella, Dobra and Edina, Gyukity-Sebestyen and Buzás, Krisztina},
	doi = {10.1002/jev2.12224},
	journal-iso = {J EXTRACELLULAR VESICL},
	journal = {JOURNAL OF EXTRACELLULAR VESICLES},
	volume = {11},
	unique-id = {33609933},
	year = {2022},
	eissn = {2001-3078},
	pages = {222},
	orcid-numbers = {Buzás, Krisztina/0000-0001-8933-2033}
}