TY - CHAP AU - Major, Fanni AU - Pintér, Tímea AU - Erdélyi, Márta AU - Balogh, Krisztián Milán AU - Petheő, Gábor L. AU - Geiszt, Miklós AU - Bodrogi, Lilla ED - Hagymási, Krisztina ED - Janda, Tibor ED - Pál, Magda ED - Poór, Péter ED - Szalai, Gabriella TI - Hozzáadott koleszterint tartalmazó takarmány fogyasztásának hatása a szervezet antioxidáns rendszerére NOX4 génkiütött nyulakban T2 - A Magyar Szabadgyökkutató Társaság XII. Kongresszusa PB - Agrártudományi Kutatóközpont, Mezőgazdasági Intézet CY - Martonvásár SN - 9786156203021 PY - 2023 SP - 42 PG - 1 UR - https://m2.mtmt.hu/api/publication/34529069 ID - 34529069 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Pintér, Tímea AU - Major, Fanni AU - Urbán, Martin AU - Erdélyi, Márta AU - Balogh, Krisztián Milán AU - Skoda, Gabriella AU - Kerekes, Andrea AU - Lipták, Nándor AU - Petheő, Gábor L. AU - Szeles, Zsolt AU - Gócza, Elen AU - Hiripi, László AU - Hoffmann, Orsolya Ivett AU - Szőke, Zsuzsanna AU - Geiszt, Miklós AU - Bodrogi, Lilla ED - Hagymási, Krisztina ED - Janda, Tibor ED - Pál, Magda ED - Poór, Péter ED - Szalai, Gabriella TI - Genomszerkesztéssel előállított nyúlmodellek a szabadgyökkutatásban T2 - A Magyar Szabadgyökkutató Társaság XII. Kongresszusa PB - Agrártudományi Kutatóközpont, Mezőgazdasági Intézet CY - Martonvásár SN - 9786156203021 PY - 2023 SP - 51 PG - 1 UR - https://m2.mtmt.hu/api/publication/34528981 ID - 34528981 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Janovicz, Anna AU - Majer, Aliz AU - Kosztelnik, Mónika AU - Geiszt, Miklós AU - Chun, Jerold AU - Ishii, Satoshi AU - Tigyi, Gabor AU - Benyó, Zoltán AU - Ruisanchez, Éva TI - Autotaxin–lysophosphatidic acid receptor 5 axis evokes endothelial dysfunction via reactive oxygen species signaling JF - EXPERIMENTAL BIOLOGY AND MEDICINE J2 - EXP BIOL MED VL - 248 PY - 2023 IS - 20 SP - 1887 EP - 1894 PG - 8 SN - 1535-3702 DO - 10.1177/15353702231199081 UR - https://m2.mtmt.hu/api/publication/34213267 ID - 34213267 AB - Lysophosphatidylcholine (LPC) is a bioactive lipid that has been shown to attenuate endothelium-dependent vasorelaxation contributing to endothelial dysfunction; however, the underlying mechanisms are not well understood. In this study, we investigated the molecular mechanisms involved in the development of LPC-evoked impairment of endothelium-dependent vasorelaxation. In aortic rings isolated from wild-type (WT) mice, a 20-min exposure to LPC significantly reduced the acetylcholine chloride (ACh)–induced vasorelaxation indicating the impairment of normal endothelial function. Interestingly, pharmacological inhibition of autotaxin (ATX) by GLPG1690 partially reversed the endothelial dysfunction, suggesting that lysophosphatidic acid (LPA) derived from LPC may be involved in the effect. Therefore, the effect of LPC was also tested in aortic rings isolated from different LPA receptor knock-out (KO) mice. LPC evoked a marked reduction in ACh-dependent vasorelaxation in Lpar1, Lpar2, and Lpar4 KO, but its effect was significantly attenuated in Lpar5 KO vessels. Furthermore, addition of superoxide dismutase reduced the LPC-induced endothelial dysfunction in WT but not in the Lpar5 KO mice. In addition, LPC increased H 2 O 2 release from WT vessels, which was significantly reduced in Lpar5 KO vessels. Our findings indicate that the ATX–LPA–LPA 5 receptor axis is involved in the development of LPC-induced impairment of endothelium-dependent vasorelaxation via LPA 5 receptor–mediated reactive oxygen species production. Taken together, in this study, we identified a new pathway contributing to the development of LPC-induced endothelial dysfunction. LA - English DB - MTMT ER - TY - JOUR AU - Szeles, Zsolt AU - Petheő, Gábor L. AU - Szikora, Bence AU - Kacskovics, Imre AU - Geiszt, Miklós TI - A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-44018-5 UR - https://m2.mtmt.hu/api/publication/34198665 ID - 34198665 AB - Members of the NOX/DUOX family of NADPH oxidases are responsible for regulated ROS production in diverse cells and tissues. Detection of NOX/DUOX proteins at the protein level remains an important challenge in the field. Here we report the development and characterization of a novel anti-NOX5 monoclonal antibody, which recognizes the human NOX5 protein in both Western blot, immunocytochemistry, and histochemistry applications. With the help of the antibody we could successfully detect both heterologously and endogenously expressed NOX5 in mammalian cells. Furthermore, we could also detect NOX5 protein in the human spleen, testis, and ovary. Immunohistochemical studies on human testis revealed that NOX5 localized to spermatogenic cells. This expression pattern was also supported by the result of in silico analysis of single-cell RNA sequencing data that indicated that NOX5 protein is present in developing spermatids and spermatocytes. Mature spermatozoa, however, did not contain detectable NOX5. In the human ovary, both immunostaining and single-cell RNA sequencing suggest that NOX5 is expressed in interstitial fibroblasts and theca cells. We also analyzed vascular cells for the presence of NOX5 and we found that NOX5 expression is a fairly specific feature of splenic endothelial cells. LA - English DB - MTMT ER - TY - CHAP AU - Leto, T.L. AU - Geiszt, Miklós ED - Edgar, Pick TI - NADPH Oxidase 1: At the Interface of the Intestinal Epithelium and Gut Microbiota T2 - NADPH Oxidases Revisited: From Function to Structure PB - Springer International Publishing CY - Cham SN - 9783031237515 T3 - NADPH Oxidases Revisit.: From Function to Structure PY - 2023 SP - 147 EP - 163 PG - 17 DO - 10.1007/978-3-031-23752-2_10 UR - https://m2.mtmt.hu/api/publication/34157366 ID - 34157366 LA - English DB - MTMT ER - TY - CONF AU - Durst, Máté AU - Sípos, Klaudia AU - Matuska, Rita AU - Várnai, Péter AU - Soltész-Katona, Eszter AU - Geiszt, Miklós AU - Tóth, Zsuzsanna TI - Involvement of nesfatin-1/NUCB2 in glucose homeostasis in the arcuate nucleus T2 - Joint Neuroscience Meeting of the Hungarian Neuroscience Society (MITT) & the Austrian Neuroscience Association (ANA) PY - 2023 UR - https://m2.mtmt.hu/api/publication/33780242 ID - 33780242 LA - English DB - MTMT ER - TY - JOUR AU - Pató, Anna Terézia AU - Bölcskei, Kata AU - Donkó, Ágnes AU - Kaszás, Diána Patricia AU - Boros, Melinda AU - Bodrogi, Lilla AU - Várady, György AU - Pape, Veronika AU - Roux, Benoit AU - Enyedi, Balázs AU - Helyes, Zsuzsanna AU - Watt, F.M. AU - Sirokmány, Gábor AU - Geiszt, Miklós TI - Hydrogen peroxide production by epidermal dual oxidase 1 regulates nociceptive sensory signals JF - REDOX BIOLOGY J2 - REDOX BIOL VL - 62 PY - 2023 PG - 13 SN - 2213-2317 DO - 10.1016/j.redox.2023.102670 UR - https://m2.mtmt.hu/api/publication/33718115 ID - 33718115 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Habibovic, A. AU - Hristova, M. AU - Morris, C.R. AU - Lin, M.-C.J. AU - Cruz, L.C. AU - Ather, J.L. AU - Geiszt, Miklós AU - Anathy, V. AU - Janssen-Heininger, Y.M.W. AU - Poynter, M.E. AU - Dixon, A.E. AU - van, der Vliet A. TI - Diet-induced obesity worsens allergen-induced type 2/type 17 inflammation in airways by enhancing DUOX1 activation JF - AMERICAN JOURNAL OF PHYSIOLOGY: LUNG CELLULAR AND MOLECULAR PHYSIOLOGY J2 - AM J PHYSIOL LUNG C VL - 324 PY - 2023 IS - 2 SP - L228 EP - L242 SN - 1040-0605 DO - 10.1152/ajplung.00331.2022 UR - https://m2.mtmt.hu/api/publication/33666662 ID - 33666662 LA - English DB - MTMT ER - TY - GEN AU - Pintér, Tímea AU - Muhammad, F AU - Major, Fanni AU - Petheő, Gábor L. AU - Szeles, Zsolt AU - Skoda, Gabriella AU - Kerekes, Andrea AU - Hiripi, László AU - Geiszt, Miklós AU - Bodrogi, Lilla TI - Examination of the antioxidant system in knock-out rabbits on cholesterolrich diet PY - 2022 SP - 110 UR - https://m2.mtmt.hu/api/publication/33602930 ID - 33602930 LA - English DB - MTMT ER - TY - CHAP AU - Pintér, Tímea AU - Lóki, Lilla AU - Petheő, Gábor L. AU - Szeles, Zsolt AU - Skoda, Gabriella AU - Kerekes, Andrea AU - Hiripi, László AU - Geiszt, Miklós AU - Bodrogi, Lilla ED - Bánfalvi, Zsófia ED - Gócza, Elen ED - Olasz, Ferenc ED - Pál, Magda ED - Posta, Katalin ED - Várallyay, Éva TI - The effect of cholesterol-rich diet on the antioxidant system of NOX5-deficient rabbit T2 - „FIBOK 2022” : Fiatal Biotechnológusok V. Országos Konferenciája PB - MATE Genetika és Biotechnológia Intézet CY - Gödöllő SN - 9789632699998 PY - 2022 SP - 121 EP - 121 PG - 1 UR - https://m2.mtmt.hu/api/publication/33299292 ID - 33299292 LA - Hungarian DB - MTMT ER -