@article{MTMT:32201080,
	title = {The retention of Zr from potential therapeutic silica-zirconia core-shell nanoparticles in aquatic organisms},
	url = {https://m2.mtmt.hu/api/publication/32201080},
	author = {Sajtos, Zsófi and Fehér, Milán and Molnár, Áron and Stündl, László and Nagyné Naszályi, Lívia and Martins, José C. and Harangi, Sándor and Magyar, István and Fehér, Krisztina and Baranyai, Edina},
	doi = {10.1016/j.enmm.2021.100572},
	journal-iso = {ENVIRON NANOTECH MONITOR MANAG},
	journal = {ENVIRONMENTAL NANOTECHNOLOGY MONITORING AND MANAGEMENT},
	volume = {16},
	unique-id = {32201080},
	abstract = {Model experiments under laboratory conditions were carried out to assess the accumulation tendency of Zr from a silica-zirconia core-shell nanoparticles, synthesised for the assay. Acute exposition tests were conducted with Daphnia magna and Danio rerio and the accumulation tendency of the nano and the molecular form was compared. Significant elevation of Zr was found in the tissue of the test organisms treated by the NP compared to the control, however, the retention of zebrafish was lower than that of the daphnids. Increased level of bioconcentration factor and the strong correlation of the redundancy analysis data suggest accumulation tendency in D. magna, yet long-term experiments are required to prove and further assess the environmental risk of food-chain bioconcentration. The BCF results were under the REACH limit for the D. rerio groups indicating the low short-term accumulation tendency of Zr from the NP. However, Zr level was significantly higher in zebrafish individuals originating from the SiO2@ZrO2 NP exposed treatments compared to the groups supplemented by the same concentration of ZrOCl2, which did not result in the elevation of Zr in fish tissue. The trace element homeostasis of D. rerio was not affected by the acute SiO2@ZrO2 NP exposition and no lethality occurred.},
	year = {2021},
	eissn = {2215-1532},
	orcid-numbers = {Fehér, Milán/0000-0002-4816-9329; Nagyné Naszályi, Lívia/0000-0003-2799-1150}
}

@article{MTMT:32164498,
	title = {Silica@zirconia Core@shell Nanoparticles for Nucleic Acid Building Block Sorption},
	url = {https://m2.mtmt.hu/api/publication/32164498},
	author = {Nagyné Naszályi, Lívia and Dhaene, Evert and Van Zele, Matthias and Mihály, Judith and Klébert, Szilvia and Varga, Zoltán and E Kövér, Katalin and De Buysser, Klaartje and Van Driessche, Isabel and Martins, José C. and Fehér, Krisztina},
	doi = {10.3390/nano11092166},
	journal-iso = {NANOMATERIALS-BASEL},
	journal = {NANOMATERIALS},
	volume = {11},
	unique-id = {32164498},
	abstract = {The development of delivery systems for the immobilization of nucleic acid cargo molecules is of prime importance due to the need for safe administration of DNA or RNA type of antigens and adjuvants in vaccines. Nanoparticles (NP) in the size range of 20–200 nm have attractive properties as vaccine carriers because they achieve passive targeting of immune cells and can enhance the immune response of a weakly immunogenic antigen via their size. We prepared high capacity 50 nm diameter silica@zirconia NPs with monoclinic/cubic zirconia shell by a green, cheap and up-scalable sol–gel method. We studied the behavior of the particles upon water dialysis and found that the ageing of the zirconia shell is a major determinant of the colloidal stability after transfer into the water due to physisorption of the zirconia starting material on the surface. We determined the optimum conditions for adsorption of DNA building blocks, deoxynucleoside monophosphates (dNMP), the colloidal stability of the resulting NPs and its time dependence. The ligand adsorption was favored by acidic pH, while colloidal stability required neutral-alkaline pH; thus, the optimal pH for the preparation of nucleic acid-modified particles is between 7.0–7.5. The developed silica@zirconia NPs bind as high as 207 mg dNMPs on 1 g of nanocarrier at neutral-physiological pH while maintaining good colloidal stability. We studied the influence of biological buffers and found that while phosphate buffers decrease the loading dramatically, other commonly used buffers, such as HEPES, are compatible with the nanoplatform. We propose the prepared silica@zirconia NPs as promising carriers for nucleic acid-type drug cargos.},
	year = {2021},
	eissn = {2079-4991},
	orcid-numbers = {Nagyné Naszályi, Lívia/0000-0003-2799-1150; Dhaene, Evert/0000-0002-1604-0408; Van Zele, Matthias/0000-0001-5809-4108; Klébert, Szilvia/0000-0002-3107-3371; Varga, Zoltán/0000-0002-5741-2669; De Buysser, Klaartje/0000-0001-7462-2484; Martins, José C./0000-0001-7350-2253}
}

@article{MTMT:31312924,
	title = {An unsought and expensive way to make gold nanoparticles on the way to the development of SiO2@ZrO2 nanocarriers for cancer vaccination},
	url = {https://m2.mtmt.hu/api/publication/31312924},
	author = {Nagyné Naszályi, Lívia and Dhaene, E and Szigyártó, Imola Csilla and Mihály, Judith and May, Zoltán and Varga, Zoltán and Van Driessche, I and Martins, JC. and Fehér, Krisztina},
	doi = {10.1016/j.molliq.2020.113307},
	journal-iso = {J MOL LIQ},
	journal = {JOURNAL OF MOLECULAR LIQUIDS},
	volume = {311},
	unique-id = {31312924},
	issn = {0167-7322},
	abstract = {Silica@zirconia core@shell oxide nanoparticles of approx. 50 nm diameter were developed in view of an application as a novel carrier with adjuvant activities for cancer vaccines. To this end, the aim was to cover the surface of the nanocarrier by potent single stranded DNA (ssDNA) type immune stimulators used in cancer vaccination. Prior to characterizing the binding of ssDNA to the nanocarrier surface, the adsorption of deoxynucleoside monophosphate building blocks (dNMPs) was studied. After optimization of conditions for synthesis, solvent exchange and surface modification, the effects and possible interference of several buffers, such as HEPES, PIPES, MOPS and MOPSO, were also investigated. Formation of a new compound was revealed by UV–VIS spectroscopy during the recording of zeta potential vs. pH titration curves in the presence of HEPES and PIPES buffer in the neutral-acidic pH region. We identified this new species as gold ions etched from the electrode of the zeta cuvette, stabilized by the buffers via chelation, which is then followed by their conversion into gold nanoparticles.},
	year = {2020},
	eissn = {1873-3166},
	orcid-numbers = {Nagyné Naszályi, Lívia/0000-0003-2799-1150; Varga, Zoltán/0000-0002-5741-2669}
}

@misc{MTMT:30452624,
	title = {Ceramic core@shell nanospheres as vaccine carriers},
	url = {https://m2.mtmt.hu/api/publication/30452624},
	author = {Nagyné Naszályi, Lívia and Rein, Verbeke and Heleen, Dewitte and Fehér, Krisztina and Stefaan, De Smedt and José, Martins},
	unique-id = {30452624},
	abstract = {The need for new vaccines is evident, but avoiding side effects is crucial for patient compliance. To achieve this, new generation vaccines are being developed. They combine an antigen as a target with the inclusion of an immunostimulant to ensure the induction of immunity, rather than tolerance. 
		Nanoparticles (NPs) are also used as vaccine-delivery systems. With appropriate size and well-chosen injection they are prone to accumulate in immune cells. However, their successful integration into nanovaccines requires thorough analysis of the inorganic/organic interface. 
		I aimed at the elaboration of new vaccine carrier nanosystems, characterized them first by routinely used analytical methods and then by the recently developed solution state NMR spectroscopy toolbox developed at Ghent University [1].
		The synthesis of silica@zirconia core@shell nanoparticles was based on Stöber et al. and Kim and coworkers’ method [2, 3]. I decorated the surface of these ceramic NPs with model biomolecules and immune stimulators. The physico-chemical characterization of the particles was carried out using transmission electron microscopy, dynamic light scattering, zeta potential measurements, UV-visible and Fourier-transform infrared spectroscopies. The surface ligand structure was studied by NMR toolbox. Preliminary experiments on biological fate of the as-prepared NPs were carried out (blood stability tests, cell viability assays etc).},
	year = {2018},
	orcid-numbers = {Nagyné Naszályi, Lívia/0000-0003-2799-1150}
}

@article{MTMT:3409584,
	title = {Role of oligo(malic acid) on the formation of unilamellar vesicles},
	url = {https://m2.mtmt.hu/api/publication/3409584},
	author = {Bóta, Attila and Wacha, András Ferenc and Varga, Zoltán and Szigyártó, Imola Csilla and Kristyán, Sándor and Lőrincz, András and Szabó, Pál Tamás and Kálmán, Miklós and Nagyné Naszályi, Lívia and Mihály, Judith},
	doi = {10.1016/j.jcis.2018.08.020},
	journal-iso = {J COLLOID INTERF SCI},
	journal = {JOURNAL OF COLLOID AND INTERFACE SCIENCE},
	volume = {532},
	unique-id = {3409584},
	issn = {0021-9797},
	keywords = {freeze fracture; small-angle X-ray scattering (SAXS); Oligo(malic acid); Sterically stabilized vesicle},
	year = {2018},
	eissn = {1095-7103},
	pages = {782-789},
	orcid-numbers = {Wacha, András Ferenc/0000-0002-9609-0893; Varga, Zoltán/0000-0002-5741-2669; Szabó, Pál Tamás/0000-0003-2260-4641; Nagyné Naszályi, Lívia/0000-0003-2799-1150}
}

@article{MTMT:3215017,
	title = {Extracellular Vesicles Mediate Radiation-Induced Systemic Bystander Signals in the Bone Marrow and Spleen},
	url = {https://m2.mtmt.hu/api/publication/3215017},
	author = {Szatmári, Tünde and Kis, Dávid and Bogdandi, EN and Benedek, Anett and Bright, S and Bowler, D and Persa, Eszter and Kis, Enikő and Balogh, Andrea and Nagyné Naszályi, Lívia and Kadhim, M and Sáfrány, Géza and Lumniczky, Katalin},
	doi = {10.3389/fimmu.2017.00347},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {8},
	unique-id = {3215017},
	issn = {1664-3224},
	abstract = {Radiation-induced bystander effects refer to the induction of biological changes in cells not directly hit by radiation implying that the number of cells affected by radiation is larger than the actual number of irradiated cells. Recent in vitro studies suggest the role of extracellular vesicles (EVs) in mediating radiation-induced bystander signals, but in vivo investigations are still lacking. Here, we report an in vivo study investigating the role of EVs in mediating radiation effects. C57BL/6 mice were total-body irradiated with X-rays (0.1, 0.25, 2 Gy), and 24 h later, EVs were isolated from the bone marrow (BM) and were intravenously injected into unirradiated (so-called bystander) animals. EV-induced systemic effects were compared to radiation effects in the directly irradiated animals. Similar to direct radiation, EVs from irradiated mice induced complex DNA damage in EV-recipient animals, manifested in an increased level of chromosomal aberrations and the activation of the DNA damage response. However, while DNA damage after direct irradiation increased with the dose, EV-induced effects peaked at lower doses. A significantly reduced hematopoietic stem cell pool in the BM as well as CD4+ and CD8+ lymphocyte pool in the spleen was detected in mice injected with EVs isolated from animals irradiated with 2 Gy. These EV-induced alterations were comparable to changes present in the directly irradiated mice. The pool of TLR4-expressing dendritic cells was different in the directly irradiated mice, where it increased after 2 Gy and in the EV-recipient animals, where it strongly decreased in a dose-independent manner. A panel of eight differentially expressed microRNAs (miRNA) was identified in the EVs originating from both low- and high-dose-irradiated mice, with a predicted involvement in pathways related to DNA damage repair, hematopoietic, and immune system regulation, suggesting a direct involvement of these pathways in mediating radiation-induced systemic effects. In conclusion, we proved the role of EVs in transmitting certain radiation effects, identified miRNAs carried by EVs potentially responsible for these effects, and showed that the pattern of changes was often different in the directly irradiated and EV-recipient bystander mice, suggesting different mechanisms.},
	year = {2017},
	eissn = {1664-3224},
	orcid-numbers = {Szatmári, Tünde/0000-0001-7727-9589; Balogh, Andrea/0000-0002-3007-0793; Nagyné Naszályi, Lívia/0000-0003-2799-1150}
}

@article{MTMT:3208794,
	title = {Direct immobilization of manganese chelates on silica nanospheres for MRI applications},
	url = {https://m2.mtmt.hu/api/publication/3208794},
	author = {Pálmai, Marcell and Pethő, A and Nagyné Naszályi, Lívia and Klébert, Szilvia and May, Zoltán and Mihály, Judith and Wacha, András Ferenc and Jemnitz, Katalin and Veres, Zsuzsa and Horváth, I and Szigeti, Krisztián and Máthé, Domokos and Varga, Zoltán},
	doi = {10.1016/j.jcis.2017.03.053},
	journal-iso = {J COLLOID INTERF SCI},
	journal = {JOURNAL OF COLLOID AND INTERFACE SCIENCE},
	volume = {498},
	unique-id = {3208794},
	issn = {0021-9797},
	abstract = {The development of tissue specific magnetic resonance imaging (MRI) contrast agents (CAs) is very desirable to achieve high contrast ratio combined with excellent anatomical details. To this end, we introduce a highly effective manganese(II) containing silica material, with the aim to shorten the longitudinal (T1) relaxation time. The microporous silica nanospheres (MSNSs) with enlarged porosity and specific surface area were prepared by a surfactant assisted aqueous method. Subsequently, the surface silanol groups were amino-functionalized, reacted with diethylenetriaminepentaacetic (DTPA) dianhydride and finally deposited with Mn2+. After comprehensive characterization, the MRI properties of functionalized MSNSs were investigated. The resulting nanospheres demonstrated substantial contrast enhancement during the in vitro MRI investigations, which was also evidenced by significant contrast enhancement on T1-weighted MR images in vivo. Moreover, in vitro cytotoxicity assay of functionalized MSNSs on hepatocyte mono- and hepatocyte-Kuppfer cell co-cultures showed no significant decrease in cell viability. Our findings confirmed our hypothesis, that Mn2+-chelating MSNSs are appropriate candidates for liver-specific T1-weighted MRI CAs with high relaxivities (r1 = 7.18 mM−1 s−1). © 2017 Elsevier Inc.},
	keywords = {SILICA; Magnetic Resonance Imaging; Manganese; MRI CONTRAST AGENTS; DTPA; contrast enhancement; chelation; Nanospheres; Magnetic resonance imaging contrast agents; Silica nanospheres; Surfactant assisted; Microporous silica; High contrast ratio; Nanoparticulate MRI contrast agent; Manganese(II) chelate},
	year = {2017},
	eissn = {1095-7103},
	pages = {298-305},
	orcid-numbers = {Nagyné Naszályi, Lívia/0000-0003-2799-1150; Klébert, Szilvia/0000-0002-3107-3371; Wacha, András Ferenc/0000-0002-9609-0893; Varga, Zoltán/0000-0002-5741-2669}
}

@article{MTMT:3171083,
	title = {Preparation and 68Ga-radiolabeling of porous zirconia nanoparticle platform for PET/CT-imaging guided drug delivery},
	url = {https://m2.mtmt.hu/api/publication/3171083},
	author = {Polyák, András and Nagyné Naszályi, Lívia and Mihály, Judith and Görres, S and Wittneben, A and Leiter, I and Bankstahl, JP and Sajti, L and Kellermayer, Miklós and Zrínyi, Miklós and Ross, TL},
	doi = {10.1016/j.jpba.2017.01.028},
	journal-iso = {J PHARMACEUT BIOMED ANAL},
	journal = {JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS},
	volume = {137},
	unique-id = {3171083},
	issn = {0731-7085},
	keywords = {PET; NANOPARTICLES; DOTA; Imaging guided therapy; Theranostics; 68Ga},
	year = {2017},
	eissn = {1873-264X},
	pages = {146-150},
	orcid-numbers = {Nagyné Naszályi, Lívia/0000-0003-2799-1150; Kellermayer, Miklós/0000-0002-5553-6553; Zrínyi, Miklós/0000-0002-9362-3199}
}

@article{MTMT:3164343,
	title = {Biodistribution investigations of technetium-labelled murine bone marrow-derived extracellular vesicles by nanospect/Ct},
	url = {https://m2.mtmt.hu/api/publication/3164343},
	author = {Balogh, Andrea and Polyák, András and Péteri, A Zs and Benedek, A and Pöstényi, Zita and Nagyné Naszályi, Lívia and Balogh, Lajos and Persa, Eszter and Sáfrány, Géza and Kadhim, M and Lumniczky, Katalin},
	journal-iso = {CENTR EUR J OCUPP ENVIRON MED},
	journal = {CENTRAL EUROPEAN JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE},
	volume = {22},
	unique-id = {3164343},
	issn = {1219-1221},
	year = {2016},
	pages = {206-2016},
	orcid-numbers = {Balogh, Andrea/0000-0002-3007-0793; Nagyné Naszályi, Lívia/0000-0003-2799-1150}
}

@article{MTMT:3071427,
	title = {Silica@zirconia@poly(malic acid) nanoparticle: a promising nanocarrier for theranostic applications},
	url = {https://m2.mtmt.hu/api/publication/3071427},
	author = {Nagyné Naszályi, Lívia and Polyák, András and Mihály, Judith and Szécsényi, Á and Szigyártó, Imola Csilla and Czégény, Zsuzsanna and Pekkerné Jakab, Emma and Németh, Péter and Magda, Balázs and Szabó, Pál Tamás and Veres, Zsuzsa and Jemnitz, Katalin and Bertóti, Imre and Jóba, Róbert Péter and Trencsényi, György and Balogh, L and Bóta, Attila},
	doi = {10.1039/c6tb01102k},
	journal-iso = {J MATER CHEM B},
	journal = {JOURNAL OF MATERIALS CHEMISTRY B},
	volume = {4},
	unique-id = {3071427},
	issn = {2050-750X},
	year = {2016},
	eissn = {2050-7518},
	pages = {4420-4429},
	orcid-numbers = {Nagyné Naszályi, Lívia/0000-0003-2799-1150; Németh, Péter/0000-0001-5592-5877; Szabó, Pál Tamás/0000-0003-2260-4641; Trencsényi, György/0000-0001-6456-6212}
}