TY - JOUR AU - Dán, Kinga AU - Kocsubé, Sándor AU - Tóth, Liliána AU - Farkas, Attila AU - Rákhely, Gábor AU - Galgóczi, László Norbert TI - Isolation and identification of fungal biodeteriogens from the wall of a cultural heritage church and potential applicability of antifungal proteins in protection JF - JOURNAL OF CULTURAL HERITAGE J2 - J CULT HERIT VL - 67 PY - 2024 SP - 194 EP - 202 PG - 9 SN - 1296-2074 DO - 10.1016/j.culher.2024.03.002 UR - https://m2.mtmt.hu/api/publication/34749009 ID - 34749009 N1 - Export Date: 16 April 2024 Correspondence Address: Galgóczy, L.; Department of Biotechnology, Közép fasor 52, Hungary; email: galgoczi@bio.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - T., Kovács AU - N., Bounedjoum AU - Bali, Dominika AU - Rákhely, Gábor TI - A novel bacteriophage-based solution against Xanthomonas arboricola pv. juglandis: Natural approach for organic walnut production JF - ACTA HORTICULTURAE: TECHNICAL COMMUNICATIONS OF ISHS J2 - ACTA HORTICULTURAE PY - 2024 PG - 12 SN - 0567-7572 UR - https://m2.mtmt.hu/api/publication/34721913 ID - 34721913 LA - English DB - MTMT ER - TY - JOUR AU - Váradi, Györgyi AU - Bende, Gábor AU - Borics, Attila AU - Dán, Kinga AU - Rákhely, Gábor AU - Tóth, Gábor AU - Galgóczi, László Norbert TI - Rational Design of Antifungal Peptides Based on the γ-Core Motif of a Neosartorya (Aspergillus) fischeri Antifungal Protein to Improve Structural Integrity, Efficacy, and Spectrum JF - ACS OMEGA J2 - ACS OMEGA VL - 9 PY - 2024 IS - 6 SP - 7206 EP - 7214 PG - 9 SN - 2470-1343 DO - 10.1021/acsomega.3c09377 UR - https://m2.mtmt.hu/api/publication/34627084 ID - 34627084 N1 - Funding Agency and Grant Number: , Nemzeti Kutat?si Fejleszt?si ?s Innov?ci?s Hivatal [TKP2021-EGA-32]; Hungarian National Research, Development, and Innovation OfficeyNKFIH [FK 134343]; Hungarian National Research Development and Innovation OfficeyNKFIH; University of Szeged Open Access Fund [6653] Funding text: G.V. and G.K.T. were supported by the TKP2021-EGA-32 fund of the Hungarian National Research, Development, and Innovation OfficeyNKFIH. The present work of L.G. was financed by the Hungarian National Research Development and Innovation OfficeyNKFIH, FK 134343 project. The open-access publishing was supported by the University of Szeged Open Access Fund; grant number: 6653. LA - English DB - MTMT ER - TY - JOUR AU - Bodor, Attila AU - Feigl, Gábor AU - Kolossa, Bálint AU - Mészáros, Enikő AU - Laczi, Krisztián AU - Kovács, Etelka AU - Perei, Katalin AU - Rákhely, Gábor TI - Soils in distress: The impacts and ecological risks of (micro)plastic pollution in the terrestrial environment JF - ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY J2 - ECOTOX ENVIRON SAFE VL - 269 PY - 2024 PG - 23 SN - 0147-6513 DO - 10.1016/j.ecoenv.2023.115807 UR - https://m2.mtmt.hu/api/publication/34432038 ID - 34432038 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary (NKFIH); Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences, Hungary [UNKP-23-5-SZTE-701]; New National Excellence Program of the Ministry of Human Capacities, Hungary [FK 142475]; [BO/00181/21/4] Funding text: G. F. was supported by the National Research, Development and Innovation Office, Hungary (NKFIH FK 142475) , the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences, Hungary (Grant no. BO/00181/21/4) and the New National Excellence Program of the Ministry of Human Capacities, Hungary (UNKP-23-5-SZTE-701) . In memory of Gabor Rakhely, who passed away during the revision process of this manuscript, we honour his contributions to this work. LA - English DB - MTMT ER - TY - JOUR AU - Szuhaj, Márk AU - Kakuk, Balázs AU - Wirth, Roland AU - Rákhely, Gábor AU - Kovács, Kornél Lajos AU - Bagi, Zoltán TI - Regulation of the methanogenesis pathways by hydrogen at transcriptomic level in time JF - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY J2 - APPL MICROBIOL BIOT VL - 107 PY - 2023 IS - 20 SP - 6315 EP - 6324 PG - 10 SN - 0175-7598 DO - 10.1007/s00253-023-12700-3 UR - https://m2.mtmt.hu/api/publication/34130955 ID - 34130955 N1 - Funding Agency and Grant Number: Hungarian National Research, Development and Innovation Fund [20203.1.2-ZFR-KVG-202000009]; Hungarian NRDIF [PD 132145, K143198, FK123902, 20192.1.13-TET_IN-202000016]; University of Szeged Funding text: Open access funding provided by University of Szeged. This study has been supported in part by the Hungarian National Research, Development and Innovation Fund project 2020-3.1.2-ZFR-KVG-2020-00009. RW, ZB, and KLK received support from the Hungarian NRDIF fund projects, PD 132145, K143198, FK123902, and 2019-2.1.13-TET_IN-2020-00016. AB - The biomethane formation from 4 H-2 + CO2 by pure cultures of two methanogens, Methanocaldococcus fervens and Methanobacterium thermophilum, has been studied. The goal of the study was to understand the regulation of the enzymatic steps associated with biomethane biosynthesis by H-2, using metagenomic, pan-genomic, and transcriptomic approaches. Methanogenesis in the autotrophic methanogen M. fervens could be easily "switched off" and "switched on" by H-2/CO2 within about an hour. In contrast, the heterotrophic methanogen M. thermophilum was practically insensitive to the addition of the H-2/CO2 trigger although this methanogen also converted H-2/CO2 to CH4. From practical points of view, the regulatory function of H-2/CO2 suggests that in the power-to-gas (P2G) renewable excess electricity conversion and storage systems, the composition of the biomethane-generating methanogenic community is essential for sustainable operation. In addition to managing the specific hydrogenotrophic methanogenesis biochemistry, H-2/CO2 affected several, apparently unrelated, metabolic pathways. The redox-regulated overall biochemistry and symbiotic relationships in the methanogenic communities should be explored in order to make the P2G technology more efficient. LA - English DB - MTMT ER - TY - JOUR AU - Horváth-Gönczi, Noémi Nikolett AU - Bagi, Zoltán AU - Szuhaj, Márk AU - Rákhely, Gábor AU - Kovács, Kornél Lajos TI - Bioelectrochemical Systems (BES) for Biomethane Production-Review JF - FERMENTATION J2 - FERMENTATION-BASEL VL - 9 PY - 2023 IS - 7 PG - 33 SN - 2311-5637 DO - 10.3390/fermentation9070610 UR - https://m2.mtmt.hu/api/publication/34108330 ID - 34108330 N1 - Funding Agency and Grant Number: Hungarian National Research, Development, and Innovation Fund [2020-3.1.2-ZFR-KVG-2020-00009]; Hungarian NRDIF fund [PD132145, K143198, FK123902, 2019-2.1.13-TET_IN-2020-00016] Funding text: This study has been supported in part by the Hungarian National Research, Development, and Innovation Fund project 2020-3.1.2-ZFR-KVG-2020-00009. ZB and KLK received support from the Hungarian NRDIF fund projects PD132145, K143198, FK123902 and 2019-2.1.13-TET_IN-2020-00016. AB - Bioelectrochemical systems (BESs) have great potential in renewable energy production technologies. BES can generate electricity via Microbial Fuel Cell (MFC) or use electric current to synthesize valuable commodities in Microbial Electrolysis Cells (MECs). Various reactor configurations and operational protocols are increasing rapidly, although industrial-scale operation still faces difficulties. This article reviews the recent BES related to literature, with special attention to electrosynthesis and the most promising reactor configurations. We also attempted to clarify the numerous definitions proposed for BESs. The main components of BES are highlighted. Although the comparison of the various fermentation systems is, we collected useful and generally applicable operational parameters to be used for comparative studies. A brief overview links the appropriate microbes to the optimal reactor design. LA - English DB - MTMT ER - TY - JOUR AU - Váradi, Györgyi AU - Kele, Zoltán AU - Czajlik, András AU - Borics, Attila AU - Bende, Gábor AU - Papp, Csaba Gergő AU - Rákhely, Gábor AU - Tóth, Gábor AU - Batta, Gyula AU - Galgóczi, László Norbert TI - Hard nut to crack: Solving the disulfide linkage pattern of the Neosartorya (Aspergillus) fischeri antifungal protein 2 JF - PROTEIN SCIENCE J2 - PROTEIN SCI VL - 32 PY - 2023 IS - 7 PG - 13 SN - 0961-8368 DO - 10.1002/pro.4692 UR - https://m2.mtmt.hu/api/publication/34043893 ID - 34043893 N1 - Department of Medical Chemistry, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary Department of Organic Chemistry, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary Department of Biochemistry, Institute of Biochemistry and Molecular Biology, Semmelweis University, Budapest, Hungary Laboratory of Chemical Biology, Institute of Biochemistry, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Department of Biotechnology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary MTA-SZTE Biomimetic Systems Research Group, University of Szeged, Szeged, Hungary Fungal Genomics and Evolution Lab, Institute of Biochemistry, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary Export Date: 11 August 2023 CODEN: PRCIE Correspondence Address: Váradi, G.; Department of Medical Chemistry, Dóm tér 8, Hungary; email: varadi.gyorgyi@med.u-szeged.hu Correspondence Address: Galgóczy, L.; Department of Biotechnology, Közép fasor 52, Hungary; email: galgoczi@bio.u-szeged.hu Chemicals/CAS: disulfide, 16734-12-6; Antifungal Agents; Disulfides AB - As a consequence of the fast resistance spreading, a limited number of drugs are available to treat fungal infections. Therefore, there is an urgent need to develop new antifungal treatment strategies. The features of a disulfide bond-stabilized antifungal protein, NFAP2 secreted by the mold Neosartorya (Aspergillus) fischeri render it to be a promising template for future protein-based antifungal drug design, which requires knowledge about the native disulfide linkage pattern as it is one of the prerequisites for biological activity. However, in the lack of tryptic and chymotryptic proteolytic sites in the ACNCPNNCK sequence, the determination of the disulfide linkage pattern of NFAP2 is not easy with traditional mass spectrometry-based methods. According to in silico predictions working with a preliminary nuclear magnetic resonance (NMR) solution structure, two disulfide isomers of NFAP2 (abbacc and abbcac) were possible. Both were chemically synthesized; and comparative reversed-phase high-performance liquid chromatography, electronic circular dichroism and NMR spectroscopy analyses, and antifungal susceptibility and efficacy tests indicated that the abbcac is the native pattern. This knowledge allowed rational modification of NAFP2 to improve the antifungal efficacy and spectrum through the modulation of the evolutionarily conserved gamma-core region, which is responsible for the activity of several antimicrobial peptides. Disruption of the steric structure of NFAP2 upon gamma-core modification led to the conclusions that this motif may affect the formation of the biologically active three-dimensional structure, and that the gamma-core modulation is not an efficient tool to improve the antifungal efficacy or to change the antifungal spectrum of NFAP2. LA - English DB - MTMT ER - TY - CHAP AU - Dán, Kinga AU - Zoltán, Kele AU - Tóth, Liliána AU - Fanni, Marcsisák AU - Gábor, K. Tóth AU - Rákhely, Gábor AU - Galgóczi, László Norbert ED - Renata, Teparić ED - Andreja, Leboš Pavunc ED - Domagoj, Kifer TI - Intracellular ProteinTargets of Neosartorya (Aspergillus) fischeri Antifungal Proteins T2 - Power of Microbes in Industry and Environment 2023 PB - Croatian Microbiological Society CY - Zagreb SN - 9789537778194 PY - 2023 SP - 92 EP - 93 PG - 2 UR - https://m2.mtmt.hu/api/publication/34012624 ID - 34012624 LA - English DB - MTMT ER - TY - JOUR AU - Bérczi, Alajos AU - Márton, Zsuzsanna AU - Laskay, Krisztina AU - Tóth, András AU - Rákhely, Gábor AU - Duzs, Ágnes AU - Sebőkné Nagy, Krisztina AU - Páli, Tibor AU - Zimányi, László TI - Spectral and Redox Properties of a Recombinant Mouse Cytochrome b561 Protein Suggest Transmembrane Electron Transfer Function JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 5 PG - 16 SN - 1420-3049 DO - 10.3390/molecules28052261 UR - https://m2.mtmt.hu/api/publication/33687467 ID - 33687467 N1 - Funding Agency and Grant Number: Economic Development Operational Program of Hungary [GINOP 2.3.2-15-2016-00001, GINOP 2.3.2-15-2016-00060]; National Research, Development and Innovation Office of Hungary [OTKA K-108697, OTKA K-116323] Funding text: This research was funded by the Economic Development Operational Program of Hungary, grant numbers GINOP 2.3.2-15-2016-00001 and GINOP 2.3.2-15-2016-00060, as well as by the National Research, Development and Innovation Office of Hungary, grant numbers OTKA K-108697 and OTKA K-116323. AB - Cytochrome b561 proteins (CYB561s) are integral membrane proteins with six trans-membrane domains, two heme-b redox centers, one on each side of the host membrane. The major characteristics of these proteins are their ascorbate reducibility and trans-membrane electron transferring capability. More than one CYB561 can be found in a wide range of animal and plant phyla and they are localized in membranes different from the membranes participating in bioenergization. Two homologous proteins, both in humans and rodents, are thought to participate—via yet unidentified way—in cancer pathology. The recombinant forms of the human tumor suppressor 101F6 protein (Hs_CYB561D2) and its mouse ortholog (Mm_CYB561D2) have already been studied in some detail. However, nothing has yet been published about the physical-chemical properties of their homologues (Hs_CYB561D1 in humans and Mm_CYB561D1 in mice). In this paper we present optical, redox and structural properties of the recombinant Mm_CYB561D1, obtained based on various spectroscopic methods and homology modeling. The results are discussed in comparison to similar properties of the other members of the CYB561 protein family. LA - English DB - MTMT ER - TY - JOUR AU - Rácz, Bálint AU - Kincses, Annamária AU - Laczi, Krisztián AU - Rákhely, Gábor AU - Domínguez-Álvarez, Enrique AU - Spengler, Gabriella TI - Reversal of Multidrug Resistance by Symmetrical Selenoesters in Colon Adenocarcinoma Cells JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 2 PG - 13 SN - 1999-4923 DO - 10.3390/pharmaceutics15020610 UR - https://m2.mtmt.hu/api/publication/33641641 ID - 33641641 N1 - További támogatások: SZTE ÁOK-KKA 2018/270-62-2 of the University of Szeged, Faculty of Medicine, János Bolyai Research Scholarship (BO/00158/22/5) of the Hungarian Academy of Sciences, The study was supported by the Szeged Foundation for Cancer Research (Szegedi Rákkutatásért Alapítvány) AB - Recently, selenium containing derivatives have attracted more attention in medicinal chemistry. In the present work, the anticancer activity of symmetrical selenoesters was investigated by studying the reversal of efflux pump-related and apoptosis resistance in sensitive and resistant human colon adenocarcinoma cells expressing the ABCB1 protein. The combined effect of the compounds with doxorubicin was demonstrated with a checkerboard assay. The ABCB1 inhibitory and the apoptosis-inducing effects of the derivatives were measured with flow cytometry. Whole transcriptome sequencing was carried out on Illumina platform upon the treatment of resistant cells with the most potent derivatives. One ketone and three methyl ester selenoesters showed synergistic or weak synergistic interaction with doxorubicin, respectively. Ketone selenoesters were the most potent ABCB1 inhibitors and apoptosis inducers. Nitrile selenoesters could induce moderate early and late apoptotic processes that could be explained by their ABCB1 modulating properties. The transcriptome analysis revealed that symmetrical selenoesters may influence the redox state of the cells and interfere with metastasis formation. It can be assumed that these symmetrical selenocompounds possess toxic, DNA-damaging effects due to the presence of two selenium atoms in the molecule, which may be augmented by the presence of symmetrical groups. LA - English DB - MTMT ER -