TY - JOUR AU - Magyar, Melinda AU - Akhtar, Parveen AU - Sipka, Gábor AU - Racskóné Domonkos, Ildikó AU - Han, W. AU - Li, X. AU - Han, G. AU - Shen, J.-R. AU - Lambrev, Petar AU - Garab, Győző TI - Effects of lipids on the rate-limiting steps in the dark-to-light transition of Photosystem II core complex of Thermostichus vulcanus JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 15 PY - 2024 SN - 1664-462X DO - 10.3389/fpls.2024.1381040 UR - https://m2.mtmt.hu/api/publication/34797803 ID - 34797803 N1 - Institute of Plant Biology, HUN-REN Biological Research Centre, Szeged, Hungary Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China Research Institute for Interdisciplinary Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan Department of Physics, Faculty of Science, University of Ostrava, Ostrava, Czech Republic Export Date: 16 April 2024 Correspondence Address: Garab, G.; Institute of Plant Biology, Hungary; email: garab.gyozo@brc.hu AB - In our earlier works, we have shown that the rate-limiting steps, associated with the dark-to-light transition of Photosystem II (PSII), reflecting the photochemical activity and structural dynamics of the reaction center complex, depend largely on the lipidic environment of the protein matrix. Using chlorophyll-a fluorescence transients (ChlF) elicited by single-turnover saturating flashes, it was shown that the half-waiting time (Δτ1/2) between consecutive excitations, at which 50% of the fluorescence increment was reached, was considerably larger in isolated PSII complexes of Thermostichus (T.) vulcanus than in the native thylakoid membrane (TM). Further, it was shown that the addition of a TM lipid extract shortened Δτ1/2 of isolated PSII, indicating that at least a fraction of the ‘missing’ lipid molecules, replaced by detergent molecules, caused the elongation of Δτ1/2. Here, we performed systematic experiments to obtain information on the nature of TM lipids that are capable of decreasing Δτ1/2. Our data show that while all lipid species shorten Δτ1/2, the negatively charged lipid phosphatidylglycerol appears to be the most efficient species – suggesting its prominent role in determining the structural dynamics of PSII reaction center. Copyright © 2024 Magyar, Akhtar, Sipka, Domonkos, Han, Li, Han, Shen, Lambrev and Garab. LA - English DB - MTMT ER - TY - JOUR AU - Benyó, Dániel AU - Bato, Emese AU - Faragó, Dóra AU - Rigó, Gábor AU - Racskóné Domonkos, Ildikó AU - Labhane, Nitin AU - Zsigmond, Laura AU - Prasad, Melvin AU - Nagy, István AU - Szabados, László TI - The zinc finger protein 3 of Arabidopsis thaliana regulates vegetative growth and root hair development JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 14 PY - 2024 PG - 16 SN - 1664-462X DO - 10.3389/fpls.2023.1221519 UR - https://m2.mtmt.hu/api/publication/34547379 ID - 34547379 N1 - Funding Agency and Grant Number: Young Researcher Fellowship of the Hungarian Academy of Sciences [NKFI NN-118089, NKFI K-128728, NKFI K-143620, GINOP-2.3.3-15-2016-00023, TT_IN-2020-00034]; Tempus Fellowship Funding text: The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. Research was supported by research grants NKFI NN-118089, NKFI K-128728, NKFI K-143620, GINOP-2.3.3-15-2016-00023. TET_IN-2020-00034. DB and DF were supported by Young Researcher Fellowship of the Hungarian Academy of Sciences. NL was supported by Tempus Fellowship. AB - Introduction Zinc finger protein 3 (ZFP3) and closely related C2H2 zinc finger proteins have been identified as regulators of abscisic acid signals and photomorphogenic responses during germination. Whether ZFP3 and related ZFP factors regulate plant development is, however, not known.Results ZFP3 overexpression reduced plant growth, limited cell expansion in leaves, and compromised root hair development. The T-DNA insertion zfp3 mutant and transgenic lines with silenced ZFP1, ZFP3, ZFP4, and ZFP7 genes were similar to wild-type plants or had only minor differences in plant growth and morphology, probably due to functional redundancy. RNAseq transcript profiling identified ZFP3-controlled gene sets, including targets of ABA signaling with reduced transcript abundance. The largest gene set that was downregulated by ZFP3 encoded regulatory and structural proteins in cell wall biogenesis, cell differentiation, and root hair formation. Chromatin immunoprecipitation confirmed ZFP3 binding to several target promoters.Discussion Our results suggest that ZFP3 and related ZnF proteins can modulate cellular differentiation and plant vegetative development by regulating the expression of genes implicated in cell wall biogenesis. LA - English DB - MTMT ER - TY - JOUR AU - Szűts, Viktória AU - Kelemen-Valkony, Ildikó AU - Ötvös, Ferenc AU - Repas, Zoltan AU - Kovács, András Sándor AU - Tóth, László AU - Kiss, András Attila AU - Lőrincz, Ádám AU - Szabó, P. Balázs AU - Gajdán, Krisztina AU - Kertai, Zoltán AU - Houshmand, Nazanin AU - Szutcs, Marcella AU - Obistioui, Diana AU - Velciov, Ariana B. AU - Halasy, Katalin AU - Deim, Zoltán AU - Deák, Dalma AU - Gál, József AU - Csanádi, József AU - Racskóné Domonkos, Ildikó TI - Life style and structure differences between species of freshwater fish JF - MEDICINA INTERNACIA REVUO J2 - MED INT REV VL - 30 PY - 2022 IS - 119 SP - 98 EP - 106 PG - 9 SN - 0465-5435 UR - https://m2.mtmt.hu/api/publication/33713093 ID - 33713093 LA - English DB - MTMT ER - TY - CONF AU - Jakabné Sándor, Zsuzsanna AU - Ardó, László AU - Kanna, Sai Divya AU - Racskóné Domonkos, Ildikó AU - Ughy, Bettina AU - Perjéssy, Judit AU - Kóbori, Tímea Ottilia AU - Dergez, Ágnes Karolina TI - BIOACTIVE COMPOUNDS IN NEWLY ISOLATED FRESHWATER MICROALGAE STRAINS: IMPACT TO THE FLESH QUALITY OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) T2 - XX. TOWARDS PRECISION FISH NUTRITION AND FEEDING ( International Symposium on Fish Nutrition and Feeding PY - 2022 SP - 56 UR - https://m2.mtmt.hu/api/publication/32871973 ID - 32871973 LA - English DB - MTMT ER - TY - JOUR AU - Pleckaitis, Marijus AU - Habach, Fayez AU - Kontenis, Lukas AU - Steinbach, Gábor AU - Jarockyte, Greta AU - Kalnaityte, Agne AU - Racskóné Domonkos, Ildikó AU - Akhtar, Parveen AU - Alizadeh, Mehdi AU - Bagdonas, Saulius AU - Karabanovas, Vitalijus AU - Garab, Győző AU - Rotomskis, Ricardas AU - Barzda, Virginijus TI - Structure and principles of self-assembly of giant "sea urchin" type sulfonatophenyl porphine aggregates JF - NANO RESEARCH J2 - NANO RES VL - 15 PY - 2022 IS - 6 SP - 5527 EP - 5537 PG - 11 SN - 1998-0124 DO - 10.1007/s12274-021-4048-x UR - https://m2.mtmt.hu/api/publication/32763244 ID - 32763244 N1 - Funding Agency and Grant Number: European Regional Development FundEuropean Commission [01.2.2.-LMT-K-718-02-0016]; Research Council of Lithuania (LMTLT)Research Council of Lithuania (LMTLT); Natural Sciences and Engineering Research Council of Canada (NSERC)Natural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2017-06923, DGDND-2017-00099]; Hungarian Ministry for National Economy [GINOP-2.3.2-15-2016-00058, GINOP-2.3.2-15-2016-00001, ELKH KU-37/2020]; Eotvos Lorand Research Network Funding text: This work was supported by European Regional Development Fund (No. 01.2.2.-LMT-K-718-02-0016) under grant agreement with the Research Council of Lithuania (LMTLT). Support was also provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) (Nos. RGPIN-2017-06923 and DGDND-2017-00099). I. D. was supported by GINOP-2.3.2-15-2016-00058 and G. S. by GINOP-2.3.2-15-2016-00001 and ELKH KU-37/2020 grants from the Hungarian Ministry for National Economy and the Eotvos Lorand Research Network, respectively. AB - Principles of molecular self-assembly into giant hierarchical structures of hundreds of micrometers in size are studied in aggregates of meso-tetra(4-sulfonatophenyl)porphine (TPPS4). The aggregates form a central tubular core, which is covered with radially protruding filamentous non-branching aggregates. The filaments cluster and orient at varying angles from the core surface and some filaments form bundles. Due to shape resemblance, the structures are termed giant sea urchin (GSU) aggregates. Spectrally resolved fluorescence microscopy reveals J- and H-bands of TPPS4 aggregates in both the central core and the filaments. The fluorescence of the core is quenched while filaments exhibit strong fluorescence. Upon drying, the filament fluorescence gets quenched while the core is less affected, showing stronger relative fluorescence. Fluorescence-detected linear dichroism (FDLD) microscopy reveals that absorption dipoles corresponding to J-bands are oriented along the filament axis. The comparison of FDLD with scanning electron microscopy (SEM) reveals the structure of central core comprised of multilayer ribbons, which wind around the core axis forming a tube. Polarimetric second-harmonic generation (SHG) and third-harmonic generation microscopy exhibits strong signal from the filaments with nonlinear dipoles oriented close to the filament axis, while central core displays very low SHG due to close to centrosymmetric organization. Large chiral nonlinear susceptibility points to helical arrangement of the filaments. The investigation shows that TPPS4 molecules form distinct aggregate types, including chiral nanotubes and nanogranular aggregates that associate into the hierarchical GSU structure, prototypical to complex biological structures. The chiral TPPS4 aggregates can serve as harmonophores for nonlinear microscopy. LA - English DB - MTMT ER - TY - JOUR AU - Akhtar, Parveen AU - Biswas, Avratanu AU - Balog-Vig, Fanny Kata AU - Racskóné Domonkos, Ildikó AU - Kovács, László AU - Lambrev, Petar TI - Trimeric Photosystem I facilitates energy transfer from phycobilisomes in Synechocystis sp. PCC 6803 JF - PLANT PHYSIOLOGY J2 - PLANT PHYSIOL VL - 189 PY - 2022 IS - 2 SP - 827 EP - 838 PG - 12 SN - 0032-0889 DO - 10.1093/plphys/kiac130 UR - https://m2.mtmt.hu/api/publication/32749652 ID - 32749652 N1 - Funding Agency and Grant Number: National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [FK-139067, 2018-1.2.1-NKP-2018-00009]; Hungarian Ministry for National Economy [GINOP-2.3.2-152016-00058]; Eotvos Lorand Research Network [SA-76/2021] Funding text: This work was supported by the National Research, Development and Innovation Office (grants FK-139067 to P.A. and 2018-1.2.1-NKP-2018-00009 to P.H.L.), the Hungarian Ministry for National Economy (GINOP-2.3.2-152016-00058 to I.D.), and the Eotvos Lorand Research Network (SA-76/2021 to P.A.). LA - English DB - MTMT ER - TY - JOUR AU - Kanna, Sai Divya AU - Racskóné Domonkos, Ildikó AU - Kóbori, T.O. AU - Dergez, Ágnes Karolina AU - Böde, Kinga AU - Nagyapáti, Sarolta AU - Zsíros, Ottó AU - Ünnep, Renáta AU - Nagy, Gergely AU - Garab, Győző AU - Szilák, László AU - Solymosi, Katalin AU - Kovács, László AU - Ughy, Bettina TI - Salt Stress Induces Paramylon Accumulation and Fine-Tuning of the Macro-Organization of Thylakoid Membranes in Euglena gracilis Cells JF - FRONTIERS IN PLANT SCIENCE J2 - FRONT PLANT SCI VL - 12 PY - 2021 PG - 15 SN - 1664-462X DO - 10.3389/fpls.2021.725699 UR - https://m2.mtmt.hu/api/publication/32823174 ID - 32823174 LA - English DB - MTMT ER - TY - CONF AU - Jakabné Sándor, Zsuzsanna AU - Bíró, Janka AU - Kóbori, Tímea Ottilia AU - Dergez, Ágnes Karolina AU - Sai, Divya Kanna AU - Racskóné Domonkos, Ildikó AU - Ughy, Bettina AU - Ardó, László ED - European, Aquaculture Society TI - FIRST EXPERIENCE OF NEWLY ISOLATED FRESHWATER ALGAE STRAINS AS IMMUNSTIMULANTS FOR STERLET (ACIPENSER RUTHENUS) JUVENILES T2 - Aquaculture Europe 21 : Abstracts PB - European Aquaculture Society (EAS) C1 - Oostende PY - 2021 SP - 596 PG - 1 UR - https://m2.mtmt.hu/api/publication/32349113 ID - 32349113 LA - English DB - MTMT ER - TY - JOUR AU - Dlouhý, Ondřej AU - Karlický, Václav AU - Arshad, Rameez AU - Zsíros, Ottó AU - Racskóné Domonkos, Ildikó AU - Kurasová, Irena AU - Wacha, András Ferenc AU - Morosinotto, Tomas AU - Bóta, Attila AU - Kouřil, Roman AU - Špunda, Vladimír AU - Garab, Győző TI - Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane–Particles. II. Structure and Functions JF - CELLS J2 - CELLS-BASEL VL - 10 PY - 2021 IS - 9 PG - 19 SN - 2073-4409 DO - 10.3390/cells10092363 UR - https://m2.mtmt.hu/api/publication/32258462 ID - 32258462 N1 - Funding Agency and Grant Number: Czech Science FoundationGrant Agency of the Czech Republic [GACR 19-13637S]; National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [OTKA K 128679]; Hungarian Ministry for National Economy [GINOP-2.3.2-152016-00058]; project "SustES-Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions" [CZ.02.1.01/0.0/0.0/16_019/0000797]; Ministry of Education, Youth and Sports of CR within the CzeCOS program [LM2018123]; European Regional Development Fund (ERDF) project 'Plants as a tool for sustainable global development' [CZ.02.1.01/0.0/0.0/16_019/0000827]; Silesian Region [07359/2019/RRC]; University of Ostrava [SGS02/P.rF/2021]; MEYS CR [LM2015043]; CF Cryo-electron Microscopy and Tomography Funding text: This work was supported by grants from the Czech Science Foundation (GAC. R 19-13637S to G.G., V.S., V.K., I.K. and O.D.), the National Research Development and Innovation Office of Hungary (OTKA K 128679 to G.G.), the Hungarian Ministry for National Economy (GINOP-2.3.2-152016-00058 to I.D.), the project "SustES-Adaptation strategies for sustainable ecosystem services and food security under adverse environmental conditions" (CZ.02.1.01/0.0/0.0/16_019/0000797, to V.S. and V.K.), by the Ministry of Education, Youth and Sports of CR within the CzeCOS program (no. LM2018123 to I.K. and V.K.), by the European Regional Development Fund (ERDF) project `Plants as a tool for sustainable global development' (no. CZ.02.1.01/0.0/0.0/16_019/0000827), the Silesian Region (07359/2019/RRC to O.D.) and the University of Ostrava (SGS02/P.rF/2021 to O.D.). The 31P-NMR measurements were performed in the NMR Center of CERIC-ERIC. We thank CIISB research infrastructure project LM2015043, funded by MEYS CR; financial support of the measurements at the CF Cryo-electron Microscopy and Tomography is gratefully acknowledged. AB - In Part I, by using P-31-NMR spectroscopy, we have shown that isolated granum and stroma thylakoid membranes (TMs), in addition to the bilayer, display two isotropic phases and an inverted hexagonal (H-II) phase; saturation transfer experiments and selective effects of lipase and thermal treatments have shown that these phases arise from distinct, yet interconnectable structural entities. To obtain information on the functional roles and origin of the different lipid phases, here we performed spectroscopic measurements and inspected the ultrastructure of these TM fragments. Circular dichroism, 77 K fluorescence emission spectroscopy, and variable chlorophyll-a fluorescence measurements revealed only minor lipase- or thermally induced changes in the photosynthetic machinery. Electrochromic absorbance transients showed that the TM fragments were re-sealed, and the vesicles largely retained their impermeabilities after lipase treatments-in line with the low susceptibility of the bilayer against the same treatment, as reflected by our P-31-NMR spectroscopy. Signatures of H-II-phase could not be discerned with small-angle X-ray scattering-but traces of H-II structures, without long-range order, were found by freeze-fracture electron microscopy (FF-EM) and cryo-electron tomography (CET). EM and CET images also revealed the presence of small vesicles and fusion of membrane particles, which might account for one of the isotropic phases. Interaction of VDE (violaxanthin de-epoxidase, detected by Western blot technique in both membrane fragments) with TM lipids might account for the other isotropic phase. In general, non-bilayer lipids are proposed to play role in the self-assembly of the highly organized yet dynamic TM network in chloroplasts. LA - English DB - MTMT ER - TY - JOUR AU - Kanna, Sai Divya AU - Racskóné Domonkos, Ildikó AU - Hódi, Barbara AU - Tóth, Viktória AU - Nagyapáti, Sarolta AU - Böde, Kinga AU - Lajkó, Dézi AU - Ardó, László AU - Jakabné Sándor, Zsuzsanna AU - Kóbori, Tímea Ottilia AU - Dergez, Ágnes Karolina AU - Ughy, Bettina TI - A mikroalgákban rejlő biotechnológiai lehetőségek, különös tekintettel az állati takarmányozásban JF - HALÁSZATFEJLESZTÉS J2 - HALÁSZATFEJLESZTÉS VL - 38 PY - 2021 SP - 62 EP - 63 PG - 2 SN - 9789632699615 SN - 1219-4816 UR - https://m2.mtmt.hu/api/publication/32225509 ID - 32225509 LA - Hungarian DB - MTMT ER -