@article{MTMT:34743720, title = {Selenosugars targeting the infective stage of Trypanosoma brucei with high selectivity}, url = {https://m2.mtmt.hu/api/publication/34743720}, author = {Dibello, Estefanía and Oddone, Natalia and Franco, Jaime and Tóthné Illyés, Tünde Zita and Medeiros, Andrea and Kiss, Attila and Hőgye, Fanni and E Kövér, Katalin and Szilágyi, László and Comini, Marcelo A.}, doi = {10.1016/j.ijpddr.2024.100529}, journal-iso = {INT J PARASITOL-DRUG}, journal = {INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE}, volume = {24}, unique-id = {34743720}, issn = {2211-3207}, abstract = {Earlier evidences showed that diglycosyl diselenides are active against the infective stage of African trypanosomes (top hits IC50 0.5 and 1.5 μM) but poorly selective (selectivity index <10). Here we extended the study to 33 new seleno-glycoconjugates with the aim to improve potency and selectivity. Three selenoglycosides and three glycosyl selenenylsulfides displayed IC50 against bloodstream Trypanosoma brucei in the sub-μM range (IC50 0.35–0.77 μM) and four of them showed an improved selectivity (selectivity index >38-folds vs. murine and human macrohages). For the glycosyl selenylsulfides, the anti-trypanosomal activity was not significantly influenced by the nature of the moiety attached to the sulfur atom. Except for a quinoline-, and to a minor extent a nitro-derivative, the most selective hits induced a rapid (within 60 min) and marked perturbation of the LMWTredox homeostasis. The formation of selenenylsulfide glycoconjugates with free thiols has been identified as a potential mechanism involved in this process.}, keywords = {MACROPHAGE; Selenoglycosides; Redox biosensor; Oxidative stress; Bloodstream trypanosoma}, year = {2024}, eissn = {2211-3207}, orcid-numbers = {Dibello, Estefanía/0000-0001-6378-3899; Oddone, Natalia/0009-0006-4884-9398; Kiss, Attila/0000-0003-3601-5143; Comini, Marcelo A./0000-0001-5000-1333} } @article{MTMT:34081680, title = {Antifungal Activity of Glucosinolate-Derived Nitriles and Their Synergistic Activity with Glucosinolate-Derived Isothiocyanates Distinguishes Various Taxa of Brassicaceae Endophytes and Soil Fungi}, url = {https://m2.mtmt.hu/api/publication/34081680}, author = {Szűcs, Zsolt and Plaszkó, Tamás and Bódor, Eszter and Csoma, Hajnalka and Ács-Szabó, Lajos and Kiss, Attila and Vasas, Gábor and Gonda, Sándor}, doi = {10.3390/plants12142741}, journal-iso = {PLANTS-BASEL}, journal = {PLANTS-BASEL}, volume = {12}, unique-id = {34081680}, abstract = {The glucosinolates of Brassicaceae plants are converted into bioactive isothiocyanates and other volatiles during a challenge by pathogens and other biotic stressors. However, the role of alternative downstream products with weaker potency (e.g., nitriles) is far from being fully understood. This study tested the possible synergistic antifungal interaction between various glucosinolate-derived nitriles and 2-phenylethyl isothiocyanate (PEITC) on 45 fungal strains, including endophytes from horseradish roots (Brassicaceae) and soil fungi, using an airtight system enabling the accurate study of extremely volatile antifungal agents. The median minimal inhibitory concentrations (MICs) were 1.28, 6.10, 27.00 and 49.72 mM for 1H-indole-3-acetonitrile (IAN), 3-phenylpropanenitrile (PPN), 4-(methylsulfanyl)-butanenitrile (MSBN) and 3-butenenitrile (BN, = allyl cyanide), respectively. Thus, nitriles were considerably weaker antifungal agents compared to PEITC with a median MIC of 0.04 mM. For the same nitriles, the median fractional inhibitory concentration indices (FICIs) of the combinations were 0.562, 0.531, 0.562 and 0.625, respectively. Altogether, 47.7%, 56.8%, 50.0% and 27.3% of tested fungal strains showed a synergistic antifungal activity (FICI ≤ 0.5) for the nitrile–isothiocyanate combinations, respectively. Hypocreales strains showed the least sensitivity towards the GSL decomposition products and their combinations. The mean MIC values for PEITC showed 0.0679 ± 0.0358, 0.0400 ± 0.0214, 0.0319 ± 0.0087 and 0.0178 ± 0.0171 mM for Hypocreales, Eurotiales, Glomerellales and Pleosporales, respectively. In addition, nitriles, especially IAN, also showed significant differences. For the same fungi, the median FICI values fell in the ranges of 0.61–0.67, 0.52–0.61, 0.40–0.50 and 0.48–0.67, respectively, depending on the nitrile. Our results suggest that glucosinolate-derived nitriles may enhance isothiocyanate antifungal activity and that they may play an active role in shaping the plant microbiome and contribute to the filtering of microbes by plants.}, keywords = {ISOTHIOCYANATE; Antifungal activity; nitrile; ITC; Synergy; Soil fungi; Endophyte fungi}, year = {2023}, eissn = {2223-7747}, orcid-numbers = {Plaszkó, Tamás/0000-0003-4211-3823; Kiss, Attila/0000-0003-3601-5143; Gonda, Sándor/0000-0001-9776-0249} } @article{MTMT:33938541, title = {Development and Evaluation of an FDM Printed Nasal Device for CPZ Solid Nanoparticles}, url = {https://m2.mtmt.hu/api/publication/33938541}, author = {To Quoc, Thinh and Bíró, Krisztina and Pető, Ágota and Kósa, Dóra and Sinka, Dávid and Lekli, István and Kiss, Attila and Budai, István and Béresová, Monika and Vecsernyés, Miklós and Siposné Fehér, Pálma and Bácskay, Ildikó and Ujhelyi, Zoltán}, doi = {10.3390/molecules28114406}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {28}, unique-id = {33938541}, issn = {1420-3049}, abstract = {Nasal drug delivery has been a focus of scientific interest for decades. A number of drug delivery systems and devices are available and have been highly successful in providing better and more comfortable therapy. The benefits of nasal drug delivery are not in question. The nasal surface provides an excellent context for the targeted delivery of active substances. In addition to the large nasal surface area and intensive absorption, the active substances delivered through the nose overcome the blood–brain barrier and can be delivered directly to the central nervous system. Formulations for nasal administration are typically solutions or liquid dispersed systems such as emulsions or suspensions. Formulation techniques for nanostructures have recently undergone intensive development. Solid-phase heterogeneous dispersed systems represent a new direction in pharmaceutical formulations. The wide range of possible examples and the variety of excipients allow for the delivery of a wide range of active ingredients. The aim of our experimental work was to develop a solid drug delivery system that possesses all of the above-mentioned advantageous properties. In developing solid nanosystems, we not only exploited the advantages of size but also the adhesive and penetration-enhancing properties of excipients. During formulation, several amphiphilic compounds with adhesion properties and penetration enhancing effects were incorporated. We used chlorpromazine (CPZ), which is mainly used in the treatment of psychotic disorders such as schizophrenia and bipolar disorder. Chlorpromazine has been previously investigated by our team in other projects. With the availability of previous methods, the analytical characterization of the drug was carried out effectively. Due to the frequent and severe side effects of the drug, the need for therapeutic dose reduction is indisputable. In this series of experiments, we succeeded in constructing drug delivery systems. Finely divided Na nanoparticles were formed using a Büchi B90 nanospray dryer. An important step in the development of the drug carrier was the selection of suitable inert carrier compounds. Particle size determination and particle size distribution analysis were performed to characterize the prepared nanostructures. As safety is the most important aspect of any drug formulation, all components and systems were tested with different biocompatibility assays. The tests performed demonstrated the safe applicability of our systems. The bioavailability of chlorpromazine was studied as a function of the ratio of the active ingredient administered nasally and intravenously. As described above, most nasal formulations are liquids, but our system is solid, so there is currently no tool available to accurately target this system. As a supplement of the project, a nasal dosing device was developed, corresponding to the anatomical structure; a prototype of the device was made using 3D FDM technology. Our results lay the foundation for the design and industrial scaling of a new approach to the design and production of a high-bioavailability nasal medicinal product.}, keywords = {chlorpromazine; Nanostructures; spray drying; TEER; penetration enhancers; MTT test; cytotoxicity investigation; RPMI cells}, year = {2023}, eissn = {1420-3049}, orcid-numbers = {Kiss, Attila/0000-0003-3601-5143; Budai, István/0000-0002-8966-3817; Béresová, Monika/0000-0001-8610-3788; Bácskay, Ildikó/0000-0001-8663-2890} } @article{MTMT:33683947, title = {Multifaceted Domino Knoevenagel‐Cyclization Reactions; Four Movements for 2H‐Chromenes and Chromans}, url = {https://m2.mtmt.hu/api/publication/33683947}, author = {Király, Sándor Balázs and Tóth, László and Kovács, Tibor and Bényei, Attila Csaba and Lisztes, Erika and Tóth, Balázs István and Bíró, Tamás and Kiss, Attila and E Kövér, Katalin and Mándi, Attila and Kurtán, Tibor}, doi = {10.1002/adsc.202300083}, journal-iso = {ADV SYNTH CATAL}, journal = {ADVANCED SYNTHESIS & CATALYSIS}, volume = {365}, unique-id = {33683947}, issn = {1615-4150}, abstract = {Domino Knoevenagel-cyclization reactions of 2H-chromene and chroman derivatives containing o-formylaryl amine or ether side-chain was carried out to produce four series of chiral condensed heterocycles representing four novel skeletons and exhibiting antiproliferative activity. The cyclization step occurred with four different mechanisms: a concerted intramolecular hetero Diels-Alder reaction (IMHDA), a stepwise polar [2+2] cycloaddition, a [1,5]-hydride shift-6-endo cyclization or a multi-step nitro hetero Diels-Alder-ring-opening-Cadogan-type cyclization sequence. The latter reaction provided a new route to hydroxyindoles by an inverse Cadogan-type cyclization, in which the nitro group is deoxygenated by a nitro IMHDA-ring-opening sequence. The cyclization mechanisms and their stereoselectivity were studied by DFT calculations, based on which we proposed a mechanism for the multi-step cyclization to hydroxyindoles and explained the observed diastereoselectivity.}, keywords = {HETEROCYCLES; configuration determination; diastereoselectivity; domino reactions; cycloaddition; MOLECULAR DIVERSITY}, year = {2023}, eissn = {1615-4169}, pages = {3301-3319}, orcid-numbers = {Kiss, Attila/0000-0003-3601-5143} } @article{MTMT:33526936, title = {Quality-controlled LC-ESI-MS food metabolomics of fenugreek (Trigonella foenum-graecum) sprouts: insights into changes in primary and specialized metabolites}, url = {https://m2.mtmt.hu/api/publication/33526936}, author = {Gonda, Sándor and Szűcs, Zsolt and Plaszkó, Tamás and Cziáky, Zoltán and Kiss, Attila and Sinka, Dávid and Bácskay, Ildikó and Vasas, Gábor}, doi = {10.1016/j.foodres.2022.112347}, journal-iso = {FOOD RES INT}, journal = {FOOD RESEARCH INTERNATIONAL}, volume = {164}, unique-id = {33526936}, issn = {0963-9969}, abstract = {Fenugreek (Trigonella foenum-graecum L.) is an important food and spice with bioactive compounds against diabetes. In this study, fenugreek seeds germinating in darkness for 72 h were studied using quantification of trigonelline and 4-hydroxyisoleucine and an LC-ESI-MS/MS-based metabolomic approach capable of accurately estimating 237 features from various primary and specialized compound classes. During germination, the concentrations of trigonelline and 4-hydroxyisoleucine rose by 33.5% and 33.3%, respectively. At the same time, untargeted metabolomics revealed 9 putative flavonoids increasing 1.19- to 2.77- fold compared to the dormant seeds. A set of 19 steroid saponins rose by 1.08- to 31.86-fold. Primary metabolites however showed much more variability: abundance changes in amino acid derivatives, peptides and saccharides fell in the 0.09- to 22.25-fold, 0.93- to 478.79-fold and 0.36- to 941.58-fold ranges, respectively. To increase biosynthesis of specialized metabolites during germination, sprouts were exposed to 1–100 mM methyl jasmonate (MeJA) and methyl salicylate (MeSA). The hormone treatments affected normal metabolism: 67.1–83.1 % and 64.1–83.5 % of compounds showed a reduction compared to the controls in 100 mM MeJA and MeSA treatments at different sampling time points. Contrary to expectations, the abundance of flavonoids decreased, compared to the control sprouts (0.75- and 0.68-fold change medians, respectively). The same was observed for most, but not all steroid saponins. The quality-controlled untargeted metabolomics approach proved to yield excellent insight into the metabolic changes during germination of fenugreek. The results suggest that although fenugreek germination causes major shifts in plant metabolism, there are no major qualitative changes in bioactive specialized metabolites during the first three days. This stability likely translates into good bioactivity that is similar to that of the seeds. Because the large changes in the primary metabolites likely alter the nutritive value of the seed, further studies are warranted.}, keywords = {Pulse; legume; seedling; germination; Untargeted metabolomics; fenugreek; sprout}, year = {2023}, eissn = {1873-7145}, orcid-numbers = {Gonda, Sándor/0000-0001-9776-0249; Plaszkó, Tamás/0000-0003-4211-3823; Kiss, Attila/0000-0003-3601-5143} } @article{MTMT:32801731, title = {Formulation, Characterization and Permeability Studies of Fenugreek (Trigonella foenum-graecum) Containing Self-Emulsifying Drug Delivery System (SEDDS)}, url = {https://m2.mtmt.hu/api/publication/32801731}, author = {Sinka, Dávid and Doma, Enikő and Szendi, Nóra and Páll, Jázmin and Kósa, Dóra and Pető, Ágota and Siposné Fehér, Pálma and Ujhelyi, Zoltán and Fenyvesi, Ferenc and Váradi, Judit and Vecsernyés, Miklós and Szűcs, Zsolt and Gonda, Sándor and Cziáky, Zoltán and Kiss, Attila and Vasas, Gábor and Bácskay, Ildikó}, doi = {10.3390/molecules27092846}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {27}, unique-id = {32801731}, issn = {1420-3049}, abstract = {Fenugreek is used as a spice and a traditional herbal medicine for a variety of purposes, given its antidiabetic and antioxidant effects. Self-emulsifying drug delivery systems (SEDDS) of herbal drugs are targets of extensive research aiming to increase bioavailability and stability. The study's objective was to formulate SEDDS containing Trigonella foenum-graecum extract to improve the stability of herbal extract and to increase their permeability through a Caco-2 monolayer. A characterized fenugreek dry extract was used for the formulations, while the SEDDS properties were examined by particle size analysis and zeta potential measurements. Permeability assays were carried out on Caco-2 cell monolayers, the integrity of which was monitored by follow-up trans-epithelial electric resistance measurements (TEER). Cytocompatibility was tested by the MTT method, and an indirect dissolution test was performed, using DPPH antioxidant reagent. Two different SEDDS compositions were formulated from a standardized fenugreek dry extract at either the micro- or the nanoemulsion scale with sufficient stability, enhanced bioavailability of the compounds, and sustained release from HPMC capsules. Based on our results, a modern, non-toxic, cytocompatible fenugreek SEDDS formulation with high antioxidant capacity was developed in order to improve the permeability and bioavailability of all components.}, keywords = {CYTOTOXICITY; Dissolution test; fenugreek; SEDDS; Trigonella foenum-graecum; Permeability assay}, year = {2022}, eissn = {1420-3049}, orcid-numbers = {Szűcs, Zsolt/0000-0002-6963-6360; Gonda, Sándor/0000-0001-9776-0249; Cziáky, Zoltán/0000-0002-1846-3236; Kiss, Attila/0000-0003-3601-5143} } @article{MTMT:31841366, title = {Basic Pharmacological Characterization of EV-34, a New H2S-Releasing Ibuprofen Derivative.}, url = {https://m2.mtmt.hu/api/publication/31841366}, author = {Gyöngyösi, Alexandra and Vivien, Verner and Bakai-Bereczki, Ilona and Kiss, Attila and Rita, Zilinyi and Tósaki, Árpád and Bak, István and Borbás, Anikó and Herczegh, Pál and Lekli, István}, doi = {10.3390/molecules26030599}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {26}, unique-id = {31841366}, issn = {1420-3049}, abstract = {Cardioprotective effects of H2S are being suggested by numerous studies. Furthermore, H2S plays a role in relaxation of vascular smooth muscle, protects against oxidative stress, and modulates inflammation. Long-term high-dose use of NSAIDs, such as ibuprofen, have been associated with enhanced cardiovascular risk. The goal of the present work is the synthesis and basic pharmacological characterization of a newly designed H2S-releasing ibuprofen derivative.Following the synthesis of EV-34, a new H2S-releasing derivative of ibuprofen, oxidative stability assays were performed (Fenton and porphyrin assays). Furthermore, stability of the molecule was studied in rat serum and liver lysates. H2S-releasing ability of the EC-34 was studied with a hydrogen sulfide sensor. MTT (3-(4,5-dimethylthiazol 2-yl)-2,5-(diphenyltetrazolium bromide)) assay was carried out to monitor the possible cytotoxic effect of the compound. Cyclooxygenase (COX) inhibitory property of EV-34 was also evaluated. Carrageenan assay was carried out to compare the anti-inflammatory effect of EV-34 to ibuprofen in rat paws.The results revealed that the molecule is stable under oxidative condition of Fenton reaction. However, EV-34 undergoes biodegradation in rat serum and liver lysates. In cell culture medium H2S is being released from EV-34. No cytotoxic effect was observed at concentrations of 10, 100, 500 µM. The COX-1 and COX-2 inhibitory effects of the molecule are comparable to those of ibuprofen. Furthermore, based on the carrageenan assay, EV-34 exhibits the same anti-inflammatory effect to that of equimolar amount of ibuprofen (100 mg/bwkg).The results indicate that EV-34 is a safe H2S releasing ibuprofen derivative bearing anti-inflammatory properties.}, keywords = {HYDROGEN-SULFIDE; Gasotransmitter; anti-inflammatory property; H2S-releasing-ibuprofen; thiolacetic acid}, year = {2021}, eissn = {1420-3049}, orcid-numbers = {Bakai-Bereczki, Ilona/0000-0003-4601-7257; Kiss, Attila/0000-0003-3601-5143; Borbás, Anikó/0000-0001-8462-4547} } @article{MTMT:31636673, title = {Synthesis and HPLC-ECD Study of Cytostatic Condensed O,N-Heterocycles Obtained from 3-Aminoflavanones}, url = {https://m2.mtmt.hu/api/publication/31636673}, author = {Szappanos, Ádám and Mándi, Attila and Gulácsi, Katalin and Lisztes, Erika and Tóth, Balázs István and Bíró, Tamás and Kónya-Ábrahám, Anita and Kiss, Attila and Bényei, Attila Csaba and Antus, Sándor and Kurtán, Tibor}, doi = {10.3390/biom10101462}, journal-iso = {BIOMOLECULES}, journal = {BIOMOLECULES}, volume = {10}, unique-id = {31636673}, issn = {2218-273X}, abstract = {Racemic chiral O,N-heterocycles containing 2-arylchroman or 2-aryl-2H-chromene subunit condensed with morpholine, thiazole or pyrrole moieties at the C-3-C-4 bond were synthesized with various substitution patterns of the aryl group by the cyclization of cis- or trans-3-aminoflavanone analogues. The 3-aminoflavanone precursors were obtained in a Neber rearrangement of oxime tosylates of flavanones, which provided the trans diastereomer as the major product and enabled the isolation of both the cis- and trans-diastereomers. The cis- and trans-aminoflavanones were utilized to prepare three diastereomers of 5-aryl-chromeno[4,3-b][1,4]oxazines. Antiproliferative activity of the condensed heterocycles and precursors was evaluated against A2780 and WM35 cancer cell lines. For a 3-(N-chloroacetylamino)-flavan-4-ol derivative, showing structural analogy with acyclic acid ceramidase inhibitors, 0.15 M, 3.50 and 6.06 M IC50 values were measured against A2780, WM35 and HaCat cell lines, and apoptopic mechanism was confirmed. Low micromolar IC50 values down to 2.14 M were identified for the thiazole- and pyrrole-condensed 2H-chromene derivatives. Enantiomers of the condensed heterocycles were separated by HPLC using chiral stationary phase, HPLC-ECD spectra were recorded and TDDFT-ECD calculations were performed to determine the absolute configuration and solution conformation. Characteristic ECD transitions of the separated enantiomers were correlated with the absolute configuration and effect of substitution pattern on the HPLC elution order was determined.}, keywords = {ANTIPROLIFERATIVE ACTIVITY; HPLC-ECD; TDDFT-ECD calculations; neber rearrangement; 3-aminoflavanones; 3-(N-chloroacetylamino)-flavan-4-ol; thiazole-condensed 2H-chromene; pyrrole-condensed 2H-chromene; lamellarin analogues}, year = {2020}, eissn = {2218-273X}, orcid-numbers = {Kiss, Attila/0000-0003-3601-5143} } @article{MTMT:31259883, title = {[1,5]-Hydride Shift-Cyclization versus C(sp2)-H Functionalization in the Knoevenagel-Cyclization Domino Reactions of 1,4- and 1,5-Benzoxazepines}, url = {https://m2.mtmt.hu/api/publication/31259883}, author = {Vargáné Szalóki, Dóra and Tóth, László and Buglyó, Balázs and Kiss, Attila and Mándi, Attila and Mátyus, Péter and Antus, Sándor and Chen, Yinghan and Li, Dehai and Tao, Lingxue and Zhang, Haiyan and Kurtán, Tibor}, doi = {10.3390/molecules25061265}, journal-iso = {MOLECULES}, journal = {MOLECULES}, volume = {25}, unique-id = {31259883}, issn = {1420-3049}, abstract = {Domino cyclization reactions of N-aryl-1,4- and 1,5-benzoxazepine derivatives involving [1,5]-hydride shift or C(sp2)-H functionalization were investigated. Neuroprotective and acetylcholinesterase activities of the products were studied. Domino Knoevenagel-[1,5]-hydride shift-cyclization reaction of N-aryl-1,4-benzoxazepine derivatives with 1,3-dicarbonyl reagents having active methylene group afforded the 1,2,8,9-tetrahydro-7bH-quinolino [1,2-d][1,4]benzoxazepine scaffold with different substitution pattern. The C(sp3)-H activation step of the tertiary amine moiety occurred with complete regioselectivity and the 6-endo cyclization took place in a complete diastereoselective manner. In two cases, the enantiomers of the chiral condensed new 1,4-benzoxazepine systems were separated by chiral HPLC, HPLC-ECD spectra were recorded, and absolute configurations were determined by time-dependent density functional theory- electronic circular dichroism (TDDFT-ECD) calculations. In contrast, the analogue reaction of the regioisomeric N-aryl-1,5-benzoxazepine derivative did not follow the above mechanism but instead the Knoevenagel intermediate reacted in an SEAr reaction [C(sp2)-H functionalization] resulting in a condensed acridane derivative. The AChE inhibitory assays of the new derivatives revealed that the acridane derivative had a 6.98 μM IC50 value.}, keywords = {TDDFT-ECD calculation; acetylcholinesterase inhibitory activity; domino Knoevenagel-[1,5]-hydride shift-cyclization; 1,4-benzoxazepine; 1,5-benzoxazepine}, year = {2020}, eissn = {1420-3049}, orcid-numbers = {Kiss, Attila/0000-0003-3601-5143; Mátyus, Péter/0000-0003-3963-9445} } @article{MTMT:31142376, title = {Chemotyping of terrestrial Nostoc-like isolates from alkali grassland areas by non-targeted peptide analysis}, url = {https://m2.mtmt.hu/api/publication/31142376}, author = {Riba, Milán and Kiss, Attila and Gonda, Sándor and Parizsa, Péter and Deák, Balázs and Török, Péter and Valkó, Orsolya and Felföldi, Tamás and Vasas, Gábor}, doi = {10.1016/j.algal.2020.101798}, journal-iso = {ALGAL RES}, journal = {ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS}, volume = {46}, unique-id = {31142376}, issn = {2211-9264}, abstract = {The Nostoc genus is a well-known heterocytous, filamentous cyanobacterium which can be found all over the world. The size of terrestrial and/or freshwater colonies can be microscopic and macroscopic as well. In addition, Nostoc species are one of the most common photosynthetic cyanobacterial partners in symbiotic interactions. Terrestrial cyanobacterial colonies were collected and isolated in this study from various alkali grassland habitats (Great Hungarian Plain). Altogether 133 colonies were isolated from the 65 collected samples. The peptide patterns of the Nostoc-like strains were examined using HPLC-ESI-MS/MS and 41 peptides were identified from 45 isolated Nostoc-like strains; these compounds belonged to 4 different peptide classes. Twelve nostoginin/microginin, 16 anabaenopeptin, 12 banyaside/suomilide variants were identified. 37% of our isolated Nostoc-like strains produced some of the peptide metabolites we tested. These strains showed distinct chemotypes according to their peptide patterns, and can be divided into 4 groups based on their metabolisms. Strains either contained: (1) nostoginins/microginins, (2) anabaenopeptins, (3) anabaenopeptins and banyasides or (4) banyasides as major compounds. Banyasides were present in many of our strains and showed very high intensity in some cases. A number of previously unknown banyaside variants have been identified.}, keywords = {Nostoc; chemotype; Saline grassland; Anabaenopeptin; Nostoginin; Banyaside}, year = {2020}, eissn = {2211-9264}, orcid-numbers = {Riba, Milán/0009-0004-5366-0456; Kiss, Attila/0000-0003-3601-5143; Gonda, Sándor/0000-0001-9776-0249; Deák, Balázs/0000-0001-6938-1997; Valkó, Orsolya/0000-0001-7919-6293; Felföldi, Tamás/0000-0003-2009-2478} }