TY - JOUR AU - Barabási, Beáta AU - Barna, Lilla AU - Santa Maria, Anaraquel AU - Harazin, András AU - Molnár, Réka AU - Kincses, András AU - Vigh, Judit Piroska AU - Dukay, Brigitta AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda AU - Walter, Fruzsina AU - Deli, Mária Anna AU - Hoyk, Zsófia TI - Role of interleukin-6 and interleukin-10 in morphological and functional changes of the blood–brain barrier in hypertriglyceridemia JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 20 PY - 2023 IS - 1 PG - 20 SN - 2045-8118 DO - 10.1186/s12987-023-00418-3 UR - https://m2.mtmt.hu/api/publication/33688118 ID - 33688118 N1 - Funding Agency and Grant Number: ELKH Biological Research Center - National Research, Development, and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00060]; European Training Network [H2020-MSCA-ITN-2015, 675619] Funding text: Open access funding provided by ELKH Biological Research Center. This work was funded by the National Research, Development, and Innovation Office of Hungary, Grant Number GINOP-2.3.2-15-2016-00060. ARSM was supported by the European Training Network H2020-MSCA-ITN-2015 [Grant Number 675619]. LA - English DB - MTMT ER - TY - JOUR AU - Kata, Diána AU - Gróf, Ilona AU - Hoyk, Zsófia AU - Ducza, Eszter AU - Deli, Mária Anna AU - Zupkó, István AU - Földesi, Imre TI - Immunofluorescent Evidence for Nuclear Localization of Aromatase in Astrocytes in the Rat Central Nervous System JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 16 PG - 21 SN - 1661-6596 DO - 10.3390/ijms23168946 UR - https://m2.mtmt.hu/api/publication/33079552 ID - 33079552 N1 - Export Date: 18 April 2023 AB - Estrogens regulate a variety of neuroendocrine, reproductive and also non-reproductive brain functions. Estradiol biosynthesis in the central nervous system (CNS) is catalyzed by the enzyme aromatase, which is expressed in several brain regions by neurons, astrocytes and microglia. In this study, we performed a complex fluorescent immunocytochemical analysis which revealed that aromatase is colocalized with the nuclear stain in glial fibrillary acidic protein (GFAP) positive astrocytes in cell cultures. Confocal immunofluorescent Z-stack scanning analysis confirmed the colocalization of aromatase with the nuclear DAPI signal. Nuclear aromatase was also detectable in the S100 beta positive astrocyte subpopulation. When the nuclear aromatase signal was present, estrogen receptor alpha was also abundant in the nucleus. Immunostaining of frozen brain tissue sections showed that the nuclear colocalization of the enzyme in GFAP-positive astrocytes is also detectable in the adult rat brain. CD11b/c labelled microglial cells express aromatase, but the immunopositive signal was distributed only in the cytoplasm both in the ramified and amoeboid microglial forms. Immunostaining of rat ovarian tissue sections and human granulosa cells revealed that aromatase was present only in the cytoplasm. This novel observation suggests a new unique mechanism in astrocytes that may regulate certain CNS functions via estradiol production. LA - English DB - MTMT ER - TY - JOUR AU - Becskeházi, Eszter AU - Korsós, Marietta Margaréta AU - Gál, Eleonóra AU - Tiszlavicz, László AU - Hoyk, Zsófia AU - Deli, Mária Anna AU - Köhler, Zoltán Márton AU - Keller-Pintér, Anikó AU - Horváth, Attila AU - Csekő, Kata AU - Helyes, Zsuzsanna AU - Hegyi, Péter AU - Venglovecz, Viktória TI - Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett’s Esophageal Cell Line JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 19 PG - 16 SN - 1661-6596 DO - 10.3390/ijms221910581 UR - https://m2.mtmt.hu/api/publication/32258572 ID - 32258572 LA - English DB - MTMT ER - TY - JOUR AU - Dulka, Karolina AU - Szabó, Melinda AU - Biróné Lajkó, Noémi AU - Belecz, István AU - Hoyk, Zsófia AU - Gulya, Károly TI - Epigenetic Consequences of in Utero Exposure to Rosuvastatin: Alteration of Histone Methylation Patterns in Newborn Rat Brains JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 7 PG - 17 SN - 1661-6596 DO - 10.3390/ijms22073412 UR - https://m2.mtmt.hu/api/publication/31997620 ID - 31997620 AB - Rosuvastatin (RST) is primarily used to treat high cholesterol levels. As it has potentially harmful but not well-documented effects on embryos, RST is contraindicated during pregnancy. To demonstrate whether RST could induce molecular epigenetic events in the brains of newborn rats, pregnant mothers were treated daily with oral RST from the 11th day of pregnancy for 10 days (or until delivery). On postnatal day 1, the brains of the control and RST-treated rats were removed for Western blot or immunohistochemical analyses. Several antibodies that recognize different methylation sites for H2A, H2B, H3, and H4 histones were quantified. Analyses of cell-type-specific markers in the newborn brains demonstrated that prenatal RST administration did not affect the composition and cell type ratios as compared to the controls. Prenatal RST administration did, however, induce a general, nonsignificant increase in H2AK118me1, H2BK5me1, H3, H3K9me3, H3K27me3, H3K36me2, H4, H4K20me2, and H4K20me3 levels, compared to the controls. Moreover, significant changes were detected in the number of H3K4me1 and H3K4me3 sites (134.3% +/- 19.2% and 127.8% +/- 8.5% of the controls, respectively), which are generally recognized as transcriptional activators. Fluorescent/confocal immunohistochemistry for cell-type-specific markers and histone methylation marks on tissue sections indicated that most of the increase at these sites belonged to neuronal cell nuclei. Thus, prenatal RST treatment induces epigenetic changes that could affect neuronal differentiation and development. LA - English DB - MTMT ER - TY - JOUR AU - Dukay, Brigitta AU - Walter, Fruzsina AU - Vigh, Judit Piroska AU - Barabási, Beáta AU - Hajdu, Petra AU - Balassa, Tamás AU - Migh, Ede AU - Kincses, András AU - Hoyk, Zsófia AU - Szögi, Titanilla AU - Borbély, Emőke AU - Csoboz, Bálint AU - Horváth, Péter AU - Fülöp, Lívia AU - Penke, Botond AU - Vigh, László AU - Deli, Mária Anna AU - Sántha, Miklós AU - Tóth, Erzsébet Melinda TI - Neuroinflammatory processes are augmented in mice overexpressing human heat-shock protein B1 following ethanol-induced brain injury JF - JOURNAL OF NEUROINFLAMMATION J2 - J NEUROINFLAMM VL - 18 PY - 2021 IS - 1 PG - 24 SN - 1742-2094 DO - 10.1186/s12974-020-02070-2 UR - https://m2.mtmt.hu/api/publication/31807147 ID - 31807147 N1 - Miklós Sántha and Melinda E. Tóth are shared last authors. LA - English DB - MTMT ER - TY - CHAP AU - Campos-Bedolla, P AU - Walter, Fruzsina AU - Del Pino, NM AU - Morales-Sosa, M AU - Hoyk, Zsófia AU - Deli, Mária Anna ED - Rojo, ME ED - Lopez, Valdes HE ED - Hernández, PA TI - Monocultivo de células endoteliales de la microvasculatura cerebral de rata T2 - Cultivo de células del sistema nervioso PB - Universidad Autónoma de Guerrero, Departamento Editorial CY - México SN - 9786079440824 PY - 2020 SP - 252 EP - 271 PG - 20 UR - https://m2.mtmt.hu/api/publication/32167190 ID - 32167190 LA - Spanish DB - MTMT ER - TY - JOUR AU - Tóth, Erzsébet Melinda AU - Dukay, Brigitta AU - Hoyk, Zsófia AU - Sántha, Miklós TI - Cerebrovascular Changes and Neurodegeneration Related to Hyperlipidemia. Characteristics of the Human ApoB-100 Transgenic Mice. TS - Characteristics of the Human ApoB-100 Transgenic Mice. JF - CURRENT PHARMACEUTICAL DESIGN J2 - CURR PHARM DESIGN VL - 26 PY - 2020 IS - 13 SP - 1486 EP - 1494 PG - 9 SN - 1381-6128 DO - 10.2174/1381612826666200218101818 UR - https://m2.mtmt.hu/api/publication/31195696 ID - 31195696 N1 - Cited By :6 Export Date: 15 September 2022 CODEN: CPDEF AB - Serum lipid levels are closely related to the structure and function of blood vessels. Chronic hyperlipidemia may lead to damage in both the cardio- and the cerebrovascular systems. Vascular dysfunctions, including impairments of the blood-brain barrier, are known to be associated with neurodegenerative diseases. A growing number of evidence suggests that cardiovascular risk factors, such as hyperlipidemia, may increase the likelihood of developing dementia. Due to differences in lipoprotein metabolism, wild-type mice are protected against diet-induced hypercholesterolemia, and their serum lipid profile is different from that observed in humans. Therefore, several transgenic mouse models have been established to study the role of different apolipoproteins and their receptors in lipid metabolism, as well as the complications related to pathological lipoprotein levels. This mini-review will focus on a transgenic mouse model overexpressing an apolipoprotein, the human ApoB-100. We will discuss literature data and current advancements on the understanding of ApoB-100 induced cardio- and cerebrovascular lesions in order to demonstrate the involvement of this type of apolipoprotein in a wide range of pathologies, and a link between hyperlipidemia and neurodegeneration. LA - English DB - MTMT ER - TY - JOUR AU - Hoyk, Zsófia AU - Tóth, Erzsébet Melinda AU - Lénárt, Nikolett AU - Nagy, Dóra AU - Dukay, Brigitta AU - Csefová, Alexandra AU - Zvara, Ágnes AU - Seprényi, György AU - Kincses, András AU - Walter, Fruzsina AU - Veszelka, Szilvia AU - Vigh, Judit Piroska AU - Barabási, Beáta AU - Harazin, András AU - Kittel, Ágnes AU - Puskás, László AU - Penke, Botond AU - Vigh, László AU - Deli, Mária Anna AU - Sántha, Miklós TI - Cerebrovascular Pathology in Hypertriglyceridemic APOB-100 Transgenic Mice JF - FRONTIERS IN CELLULAR NEUROSCIENCE J2 - FRONT CELL NEUROSCI VL - 12 ET - 0 PY - 2018 PG - 17 SN - 1662-5102 DO - 10.3389/fncel.2018.00380 UR - https://m2.mtmt.hu/api/publication/30312141 ID - 30312141 N1 - These authors have contributed equally to this work: Zsófia Hoyk, Melinda E. Tóth. Funding text: This work was supported by funding from National Research, Development and Innovation Office, Hungary (GINOP2.3.2.-15.2016-00060) and the Hungarian Basic Research Fund (OTKA NN111006). AB - Hypertriglyceridemia is not only a serious risk factor in the development of cardiovascular diseases, but it is linked to neurodegeneration, too. Previously, we generated transgenic mice overexpressing the human APOB-100 protein, a mouse model of human atherosclerosis. In this model we observed high plasma levels of triglycerides, oxidative stress, tau hyperphosphorylation, synaptic dysfunction, cognitive impairment, increased neural apoptosis and neurodegeneration. Neurovascular dysfunction is recognized as a key factor in the development of neurodegenerative diseases, but the cellular and molecular events linking cerebrovascular pathology and neurodegeneration are not fully understood. Our aim was to study cerebrovascular changes in APOB-100 transgenic mice. We described the kinetics of the development of chronic hypertriglyceridemia in the transgenic animals. Increased blood-brain barrier permeability was found in the hippocampus of APOB-100 transgenic mice which was accompanied by structural changes. Using transmission electron microscopy, we detected changes in the brain capillary endothelial tight junction structure and edematous swelling of astrocyte endfeet. In brain microvessels isolated from APOB-100 transgenic animals increased Lox-1, Aqp4, and decreased Meox-2, Mfsd2a, Abcb1a, Lrp2, Glut-1, Nos2, Nos3, Vim, and in transgenic brains reduced Cdh2 and Gfap-σ gene expressions were measured using quantitative real-time PCR. We confirmed the decreased P-glycoprotein (ABCB1) and vimentin expression related to the neurovascular unit by immunostaining in transgenic brain sections using confocal microscopy. We conclude that in chronic hypertriglyceridemic APOB-100 transgenic mice both functional and morphological cerebrovascular pathology can be observed, and this animal model could be a useful tool to study the link between cerebrovascular pathology and neurodegeneration. LA - English DB - MTMT ER - TY - JOUR AU - Veszelka, Szilvia AU - Mészáros, Mária AU - Kiss, Lóránd AU - Kóta, Zoltán AU - Páli, Tibor AU - Hoyk, Zsófia AU - Bozsó, Zsolt AU - Fülöp, Lívia AU - Tóth, András AU - Rákhely, Gábor AU - Deli, Mária Anna TI - Biotin and glutathione targeting of solid nanoparticles to cross human brain endothelial cells JF - CURRENT PHARMACEUTICAL DESIGN J2 - CURR PHARM DESIGN VL - 23 PY - 2017 IS - 28 SP - 4198 EP - 4205 PG - 8 SN - 1381-6128 DO - 10.2174/1381612823666170727144450 UR - https://m2.mtmt.hu/api/publication/3251708 ID - 3251708 LA - English DB - MTMT ER - TY - JOUR AU - Barabási, Beáta AU - Csondor, A AU - Martin-Pozas, T AU - Sanchez, AM AU - Antalffy, G AU - Siklós, László AU - Gomez-Pinedo, U AU - Párducz, Árpád AU - Hoyk, Zsófia TI - Effect of axotomy and 17beta-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice. JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 319 PY - 2016 SP - 107 EP - 115 PG - 9 SN - 0306-4522 DO - 10.1016/j.neuroscience.2016.01.049 UR - https://m2.mtmt.hu/api/publication/3013488 ID - 3013488 N1 - Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain 3DHISTECH Ltd., Konkoly-Thege M. út 29-33, Budapest, 1121, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain Cited By :2 Export Date: 26 March 2020 CODEN: NRSCD Correspondence Address: Hoyk, Z.; Laboratory for Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: hoyk.zsofia@brc.mta.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol; Receptors, Purinergic P2X7 Funding text 1: This work was supported by TÁMOP-4.2.2. A-11/1/KONV-2012-0052 and by Erasmus scholarship granted to Tamara Martín-Pozas and Adriana Marisol Pulupa Sánchez. Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain 3DHISTECH Ltd., Konkoly-Thege M. út 29-33, Budapest, 1121, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain Cited By :2 Export Date: 28 April 2020 CODEN: NRSCD Correspondence Address: Hoyk, Z.; Laboratory for Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: hoyk.zsofia@brc.mta.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol; Receptors, Purinergic P2X7 Funding text 1: This work was supported by TÁMOP-4.2.2. A-11/1/KONV-2012-0052 and by Erasmus scholarship granted to Tamara Martín-Pozas and Adriana Marisol Pulupa Sánchez. AB - The objective of the study was to examine whether axotomy and 17beta-estradiol affects P2X7 receptor expression and distribution in the hypoglossal nucleus. The left hypoglossal nerve of ovariectomized mice was cut and animals received a single injection of 17beta-estradiol (25mug/100 g b. w. in 20% (2- hydroxypropyl) - beta- cyclodextrin) or vehicle one hour after axotomy. Mice were sacrificed on day 4 following surgery. The area fraction of P2X7 receptor immunoreactive structures and of CD11b immunolabeled microglia, P2X7 protein concentration, and the immunoreactivity pattern of estrogen receptor alpha / beta were analyzed on both sides of the hypoglossal nucleus. Following axotomy the area fraction of P2X7 immunoreactive neurons showed a decreasing tendency, while the area fraction of P2X7 immunolabeled microglia increased significantly on the axotomized side compared with the control side in mice injected with vehicle. In animals treated with 17beta-estradiol the decrease in area fraction of neural and the increase in area fraction of microglial P2X7 immunostaining on the axotomized side were significantly enhanced compared with animals injected with vehicle. The P2X7 immunoreactivity pattern on the control side of the nucleus remained unchanged after 17beta-estradiol injection. Semi-quantitative Western blots revealed no significant difference in P2X7 protein concentration comparing the axotomized side with the control side in either experimental group. The CD11b immunoreactive microglia area fraction increased significantly following axotomy, but was not affected by 17beta-estradiol. Neither estrogen receptor alpha, nor beta colocalized with CD11b. Our results suggest that axotomy induces cell-type specific changes in P2X7 receptor expression, which may be directly regulated by 17beta-estradiol through estrogen receptor alpha or beta in neurons, but not in activated microglia. LA - English DB - MTMT ER -