TY - JOUR AU - Vedelek, Viktor AU - Jankovics, Ferenc AU - Zádori, János AU - Sinka, Rita TI - Mitochondrial Differentiation during Spermatogenesis: Lessons from Drosophila melanogaster JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 25 SN - 1661-6596 DO - 10.3390/ijms25073980 UR - https://m2.mtmt.hu/api/publication/34772438 ID - 34772438 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office Funding text: No Statement Available AB - Numerous diseases can arise as a consequence of mitochondrial malfunction. Hence, there is a significant focus on studying the role of mitochondria in cancer, ageing, neurodegenerative diseases, and the field of developmental biology. Mitochondria could exist as discrete organelles in the cell; however, they have the ability to fuse, resulting in the formation of interconnected reticular structures. The dynamic changes between these forms correlate with mitochondrial function and mitochondrial health, and consequently, there is a significant scientific interest in uncovering the specific molecular constituents that govern these transitions. Moreover, the specialized mitochondria display a wide array of variable morphologies in their cristae formations. These inner mitochondrial structures are closely associated with the specific functions performed by the mitochondria. In multiple cases, the presence of mitochondrial dysfunction has been linked to male sterility, as it has been observed to cause a range of abnormal spermatogenesis and sperm phenotypes in different species. This review aims to elucidate the dynamic alterations and functions of mitochondria in germ cell development during the spermatogenesis of Drosophila melanogaster. LA - English DB - MTMT ER - TY - CHAP AU - Abu Saleem, Tammam Khaliefeh Siliman AU - Molnár, Anna AU - Kovács, Vanda AU - Kiss, Karina AU - Rafael, Bence AU - SZEGEDI, Botond AU - Sinka, Rita AU - NÉMETH, Dóra AU - Homa, Mónika AU - Vágvölgyi, Csaba AU - Papp, Tamás AU - Szebenyi, Csilla AU - NÉMETH, Dóra TI - CRISPR-Cas9-based mutant library construction and characterization in Mucor lusitanicus T2 - Biotechnológiai Szakmai Nap Absztraktfüzet PB - Doktoranduszok Országos Szövetsége (DOSZ) CY - Budapest SN - 9786156457448 PY - 2024 SP - 37 EP - 39 PG - 3 UR - https://m2.mtmt.hu/api/publication/34727182 ID - 34727182 LA - English DB - MTMT ER - TY - CONF AU - Viktor, Vedelek AU - Balázs, Vedelek AU - Gabor, Juhasz AU - Péter, Lőrincz AU - Sinka, Rita TI - Convergent evolution in glutamate dehydrogenase activity in Drosophila and human T2 - 27th European Drosophila Research Conference PY - 2023 PG - 1 UR - https://m2.mtmt.hu/api/publication/34684460 ID - 34684460 LA - English DB - MTMT ER - TY - CONF AU - Bayan, Kharrat AU - Nikolett, Virág AU - Erika, Gábor AU - Ildikó, Kristó AU - Aladár, Pettkó-Szandtner AU - Sinka, Rita AU - Ferenc, Jankovics AU - Viktor, Honti TI - Dual role for the orthologue of HECA, Headcase, in blood cell progenitor maintenance in the Drosophila lymph gland T2 - 27th European Drosophila Research Conference PY - 2023 PG - 2 UR - https://m2.mtmt.hu/api/publication/34684451 ID - 34684451 LA - English DB - MTMT ER - TY - CONF AU - Sinka, Rita AU - Alzyoud, Elham AU - Dóra, Németh AU - Mónika, Krecsmarik AU - Viktor, Vedelek AU - Zsuzsánna, Réthi-Nagy AU - Zoltán, Lipinszki TI - Versatile ncMTOC organization during spermatogenesis T2 - 27th European Drosophila Research Conference PY - 2023 PG - 1 UR - https://m2.mtmt.hu/api/publication/34684442 ID - 34684442 LA - English DB - MTMT ER - TY - CHAP AU - Sinka, Rita ED - Poór, Péter TI - A Drosophila kutatás története a Szegedi Tudományegyetem Genetika Tanszékén T2 - Centenáriumi Tanulmánykötet PB - Szegedi Tudományegyetem Természettudományi és Informatikai Kar CY - Szeged SN - 9789633069134 PY - 2023 SP - 149 EP - 163 PG - 15 UR - https://m2.mtmt.hu/api/publication/34680638 ID - 34680638 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Darula, Zsuzsanna AU - Vedelek, Viktor AU - Hunyadi-Gulyás Éva, Csilla AU - Dingmann, Brian J. AU - Vedelek, Balázs AU - Kalman, Janos AU - Urban, Peter AU - Gyenesei, Attila AU - Galik-Olah, Zita AU - Gálik, Bence AU - Sinka, Rita TI - Biofilm formation initiating rotifer-specific biopolymer and its predicted components JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 253 PY - 2023 IS - Part 5 PG - 14 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2023.127157 UR - https://m2.mtmt.hu/api/publication/34334193 ID - 34334193 N1 - Megosztott szerzőség AB - The rotifer-specific biopolymer, namely Rotimer, is a recently discovered group of the biomolecule family. Rotimer has an active role in the biofilm formation initiated by rotifers (e.g., Euchlanis dilatata or Adineta vaga) or in the female-male sexual interaction of monogononts. To understand the Ca2+- and polarity-dependent formation of this multifunctional viscoelastic material, it is essential to explore its molecular composition. The investigation of the rotifer-enhanced biofilm and Rotimer-inductor conglomerate (RIC) formation yielded several protein candidates to predict the Rotimer-specific main components. The exudate of E. dilatata males was primarily applied from different biopolimer-containing samples (biofilm or RIC). The advantage of males over females lies in their degenerated digestive system and simple anatomy. Thus, their exudate is less contaminated with food and endosymbiont elements. The sequenced and annotated genome and transcriptome of this species opened the way for identifying Rotimer proteins by mass spectrometry. The predicted rotifer-biopolymer forming components are SCO-spondins and 14-3-3 protein. The characteristics of Rotimer are similar to Reissner's fiber, which is found in the central nervous system of vertebrates and is mainly formed from SCO-spondins. This molecular information serves as a starting point for its interdisciplinary investigation and application in biotechnology, biomedicine, or neurodegeneration-related drug development. LA - English DB - MTMT ER - TY - JOUR AU - Vedelek, Viktor AU - Vedelek, Balázs AU - Lőrincz, Péter AU - Juhász, Gábor AU - Sinka, Rita TI - A comparative analysis of fruit fly and human glutamate dehydrogenases in Drosophila melanogaster sperm development JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 11 PY - 2023 PG - 16 SN - 2296-634X DO - 10.3389/fcell.2023.1281487 UR - https://m2.mtmt.hu/api/publication/34239207 ID - 34239207 N1 - Funding Agency and Grant Number: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by NKFIH (PD137914 to VV, K132155 to RS, and FK138851 to PL), National Research, Development, and Innovation Offi [PD137914, K132155, FK138851]; NKFIH [2022-2.1.1-NL-2022-00008, EKA 2022/045-P101-2, LP 2022-13/2022]; National Research, Development, and Innovation Office of Hungary (Biotechnology National Laboratory) Funding text: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by NKFIH (PD137914 to VV, K132155 to RS, and FK138851 to PL), National Research, Development, and Innovation Office of Hungary (Biotechnology National Laboratory 2022-2.1.1-NL-2022-00008 to GJ), and Eotvos Lorand University Excellence Fund (EKA 2022/045-P101-2), MTA-t (LP 2022-13/2022) to PL.r The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by NKFIH (PD137914 to VV, K132155 to RS, and FK138851 to PL), National Research, Development, and Innovation Office of Hungary (Biotechnology National Laboratory 2022-2.1.1-NL-2022-00008 to GJ), and Eotvos Lorand University Excellence Fund (EKA 2022/045-P101-2), MTA-t (LP 2022-13/2022) to PL. AB - Glutamate dehydrogenases are enzymes that take part in both amino acid and energy metabolism. Their role is clear in many biological processes, from neuronal function to cancer development. The putative testis-specific Drosophila glutamate dehydrogenase, Bb8, is required for male fertility and the development of mitochondrial derivatives in spermatids. Testis-specific genes are less conserved and could gain new functions, thus raising a question whether Bb8 has retained its original enzymatic activity. We show that while Bb8 displays glutamate dehydrogenase activity, there are significant functional differences between the housekeeping Gdh and the testis-specific Bb8. Both human GLUD1 and GLUD2 can rescue the bb8 ms mutant phenotype, with superior performance by GLUD2. We also tested the role of three conserved amino acids observed in both Bb8 and GLUD2 in Gdh mutants, which showed their importance in the glutamate dehydrogenase function. The findings of our study indicate that Drosophila Bb8 and human GLUD2 could be novel examples of convergent molecular evolution. Furthermore, we investigated the importance of glutamate levels in mitochondrial homeostasis during spermatogenesis by ectopic expression of the mitochondrial glutamate transporter Aralar1, which caused mitochondrial abnormalities in fly spermatids. The data presented in our study offer evidence supporting the significant involvement of glutamate metabolism in sperm development. LA - English DB - MTMT ER - TY - JOUR AU - Réthi-Nagy, Zsuzsánna AU - Ábrahám, Edit AU - Sinka, Rita AU - Juhász, Szilvia AU - Lipinszki, Zoltán TI - Protein Phosphatase 4 Is Required for Centrobin Function in DNA Damage Repair JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 18 PG - 17 SN - 2073-4409 DO - 10.3390/cells12182219 UR - https://m2.mtmt.hu/api/publication/34129677 ID - 34129677 N1 - Export Date: 26 October 2023 Correspondence Address: Lipinszki, Z.; MTA SZBK Lendület Laboratory of Cell Cycle Regulation, Hungary; email: lipinszki.zoltan@brc.hu Correspondence Address: Juhász, S.; Institute of Biochemistry, Hungary; email: juhasz.szilvia@brc.hu AB - Genome stability in human cells relies on the efficient repair of double-stranded DNA breaks, which is mainly achieved by homologous recombination (HR). Among the regulators of various cellular functions, Protein phosphatase 4 (PP4) plays a pivotal role in coordinating cellular response to DNA damage. Meanwhile, Centrobin (CNTRB), initially recognized for its association with centrosomal function and microtubule dynamics, has sparked interest due to its potential contribution to DNA repair processes. In this study, we investigate the involvement of PP4 and its interaction with CNTRB in HR-mediated DNA repair in human cells. Employing a range of experimental strategies, we investigate the physical interaction between PP4 and CNTRB and shed light on the importance of two specific motifs in CNTRB, the PP4-binding FRVP and the ATR kinase recognition SQ sequences, in the DNA repair process. Moreover, we examine cells depleted of PP4 or CNTRB and cells harboring FRVP and SQ mutations in CNTRB, which result in similar abnormal chromosome morphologies. This phenomenon likely results from the impaired resolution of Holliday junctions, which serve as crucial intermediates in HR. Taken together, our results provide new insights into the intricate mechanisms of PP4 and CNTRB-regulated HR repair and their interrelation. LA - English DB - MTMT ER - TY - BOOK ED - László, Buday ED - Gábor, Juhász ED - Beáta, Lontay ED - József, Mihály ED - Sinka, Rita ED - László, Virág ED - Attila, Varga ED - Gergely, Szakáts TI - Hungarian Molecular Life Sciences 2023: Book of Abstracts PB - Diamond Congress Kft. CY - Budapest PY - 2023 SP - 226 SN - 9786155270772 UR - https://m2.mtmt.hu/api/publication/33806112 ID - 33806112 LA - English DB - MTMT ER -