TY - JOUR AU - Vámosi, György AU - Miller, Sarah AU - Sinha, Molika AU - Fernandez, Maria Kristha AU - Mocsár, Gábor AU - Renz, Malte TI - Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission (Apr, 10.3791/62241, 2021) JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2023 IS - 193 SP - 1 EP - 3 PG - 3 SN - 1940-087X DO - 10.3791/6546 UR - https://m2.mtmt.hu/api/publication/34352113 ID - 34352113 LA - English DB - MTMT ER - TY - GEN AU - Imre, László AU - Nánási, Péter Pál AU - Ibtissem, Benhamza AU - Kata, Nóra Enyedi AU - Mocsár, Gábor AU - Bosire, Rosevalentine AU - Hegedüs, Éva AU - Firouzi Niaki, Erfaneh AU - Csóti, Ágota AU - Zsuzsanna, Darula AU - Éva, Csősz AU - Szilárd, Póliska AU - Beáta, Scholtz AU - Gábor, Mező AU - Bacsó, Zsolt AU - T. Marc Timmers, H. AU - Masayuki, Kusakabe AU - Margit, Balázs AU - Vámosi, György AU - Juan, Ausio AU - Peter, Cheung AU - Katalin, Tóth AU - David, Tremethick AU - Masahiko, Harata AU - Szabó, Gábor TI - EPIGENETIC MODULATION VIA THE C-TERMINAL TAIL OF H2A.Z PY - 2023 SP - 2021.02.22.432230 UR - https://m2.mtmt.hu/api/publication/34191569 ID - 34191569 AB - H2A.Z-nucleosomes are present in both euchromatin and heterochromatin and it has proven difficult to interpret their disparate roles in the context of their stability features. Using an in situ assay of nucleosome stability and DT40 cells expressing engineered forms of the histone variant we show that native H2A.Z, but not C-terminally truncated H2A.Z (H2A.ZΔC), is released from nucleosomes of peripheral heterochromatin at unusually high salt concentrations. H2A.Z and H3K9me3 landscapes are reorganized in H2A.ZΔC-nuclei and overall sensitivity of chromatin to nucleases is increased. These tail-dependent differences are recapitulated upon treatment of HeLa nuclei with the H2A.Z-tail-peptide (C9), with MNase sensitivity being increased at specific regions including promoters. Introduced into live cells C9 elicits down-regulation of ∼560 genes with nonrandom chromosomal band-localization and pathway-spectrum. Thus, tail-dependent heterogeneity of H2A.Z-nucleosomes is revealed at all organization levels of chromatin and epigenetic modulation can be achieved by targeting molecular interactions involving its C-terminal tail.Competing Interest StatementThe authors have declared no competing interest. LA - English DB - MTMT ER - TY - JOUR AU - Pevná, Viktória AU - Zauška, Ľuboš AU - BENZIANE, ANASS AU - Vámosi, György AU - Girman, Vladimír AU - Miklóšová, Monika AU - Zeleňák, Vladimír AU - Huntošová, Veronika AU - Almáši, Miroslav TI - Effective transport of aggregated hypericin encapsulated in SBA-15 nanoporous silica particles for photodynamic therapy of cancer cells JF - JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY J2 - J PHOTOCH PHOTOBIO B VL - 247 PY - 2023 SN - 1011-1344 DO - 10.1016/j.jphotobiol.2023.112785 UR - https://m2.mtmt.hu/api/publication/34154220 ID - 34154220 LA - English DB - MTMT ER - TY - JOUR AU - Rehó, Bálint AU - FADEL, LINA AU - Brazda, Peter AU - BENZIANE, ANASS AU - Hegedüs, Éva AU - Sen, Pialy AU - Gadella, Theodorus W.J. AU - Tóth, Katalin AU - Nagy, László AU - Vámosi, György TI - Agonist-controlled competition of RAR and VDR nuclear receptors for heterodimerization with RXR is manifested in their DNA-binding JF - JOURNAL OF BIOLOGICAL CHEMISTRY J2 - J BIOL CHEM VL - 299 PY - 2023 IS - 2 PG - 16 SN - 0021-9258 DO - 10.1016/j.jbc.2023.102896 UR - https://m2.mtmt.hu/api/publication/33614780 ID - 33614780 AB - We found previously that nuclear receptors (NRs) compete for heterodimerization with their common partner, retinoid X receptor (RXR), in a ligand-dependent manner. To investigate potential competition in their DNA-binding, we monitored the mobility of retinoic acid receptor (RAR) and vitamin D receptor (VDR) in live cells by fluorescence correlation spectroscopy. First, specific agonist treatment and RXR co-expression additively increased RAR DNA-binding, while both agonist and RXR were required for increased VDR DNA-binding, indicating weaker DNA-binding of the VDR/RXR dimer. Second, co-expression of RAR, VDR, and RXR resulted in competition for DNA-binding. Without ligand, VDR reduced the DNA-bound fraction of RAR and vice versa, i.e., a fraction of RXR molecules was occupied by the competing partner. The DNA-bound fraction of either RAR or VDR was enhanced by its own and diminished by the competing NR's agonist. When treated with both ligands, the DNA-bound fraction of RAR increased as much as due to its own agonist, whereas that of VDR increased less. RXR agonist also increased DNA-binding of RAR at the expense of VDR. In summary, competition between RAR and VDR for RXR is also manifested in their DNA-binding in an agonist-dependent manner: RAR dominates over VDR in the absence of agonist or with both agonists present. Thus, side-effects of NR-ligand-based (retinoids, thiazolidinediones) therapies may be ameliorated by other NR ligands and be at least partly explained by reduced DNA-binding due to competition. Our results also complement the model of NR action by involving competition both for RXR and for DNA-sites. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Renátó AU - Erdélyi, Lóránd AU - Fenyvesi, Ferenc AU - Balla , Noémi AU - Kovács, Fruzsina AU - Vámosi, György AU - Klusóczki, Ágnes AU - Gyöngyösi, Alexandra AU - Bácskay, Ildikó AU - Vecsernyés, Miklós AU - Váradi, Judit TI - Concentration-Dependent Antibacterial Activity of Chitosan on Lactobacillus plantarum JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 1 SN - 1999-4923 DO - 10.3390/pharmaceutics15010018 UR - https://m2.mtmt.hu/api/publication/33434625 ID - 33434625 AB - The antimicrobial effect of chitosan and synthetic chitosan derivatives has been confirmed on many Gram-positive and Gram-negative bacteria and fungi. The tests were carried out on pathogenic microorganisms, so the mechanism and concentration dependence of the inhibitory effect of chitosan were revealed. We conducted our tests on a probiotic strain, Lactobacillus plantarum. Commercially available chitosan derivatives of different molecular weights were added to L. plantarum suspension in increasing concentrations. The minimum inhibitory concentration (MIC) value of chitosan was determined and confirmed the viability decreasing effect at concentrations above the MIC with a time-kill assay. The release of bacterium cell content was measured at 260 nm after treatment with 0.001–0.1% concentration chitosan solution. An increase in the permeability of the cell membrane was observed only with the 0.1% treatment. The interaction was also investigated by zeta potential measurement, and the irreversible interaction and concentration dependence were established in all concentrations. The interaction of fluorescein isothiocyanate (FITC) labeled low molecular weight chitosan and bacterial cells labeled with membrane dye (FM® 4–64) was confirmed by confocal microscopy. In conclusion, the inhibitory effect of chitosan was verified on a probiotic strain, which is an undesirable effect in probiotic preparations containing chitosan additives, while the inhibitory effect experienced with pathogenic strains is beneficial. LA - English DB - MTMT ER - TY - JOUR AU - Bosire, Rosevalentine AU - FADEL, LINA AU - Mocsár, Gábor AU - Nánási, Péter Pál AU - Sen, Pialy AU - Sharma, Anshu Kumar AU - Naseem, Muhammad Umair AU - Kovács, Attila AU - Kugel, Jennifer AU - Kroemer, Guido AU - Vámosi, György AU - Szabó, Gábor TI - Doxorubicin impacts chromatin binding of HMGB1, Histone H1 and retinoic acid receptor JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-022-11994-z UR - https://m2.mtmt.hu/api/publication/32832222 ID - 32832222 LA - English DB - MTMT ER - TY - JOUR AU - Sebestyén, Veronika AU - Nagy, Éva AU - Mocsár, Gábor AU - Volkó, Julianna AU - Szilágyi, Orsolya AU - Kenesei, Ádám AU - Panyi, György AU - Tóth, Katalin AU - Hajdu, Péter Béla AU - Vámosi, György TI - Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 6 PG - 15 SN - 1661-6596 DO - 10.3390/ijms23063313 UR - https://m2.mtmt.hu/api/publication/32776876 ID - 32776876 AB - Voltage-gated Kv1.3 potassium channels are essential for maintaining negative membrane potential during T-cell activation. They interact with membrane-associated guanylate kinases (MAGUK-s) via their C-terminus and with TCR/CD3, leading to enrichment at the immunological synapse (IS). Molecular interactions and mobility may impact each other and the function of these proteins. We aimed to identify molecular determinants of Kv1.3 mobility, applying fluorescence correlation spectroscopy on human Jurkat T-cells expressing WT, C-terminally truncated (Delta C), and non-conducting mutants of mGFP-Kv1.3. Delta C cannot interact with MAGUK-s and is not enriched at the IS, whereas cells expressing the non-conducting mutant are depolarized. Here, we found that in standalone cells, mobility of Delta C increased relative to the WT, likely due to abrogation of interactions, whereas mobility of the non-conducting mutant decreased, similar to our previous observations on other membrane proteins in depolarized cells. At the IS formed with Raji B-cells, mobility of WT and non-conducting channels, unlike Delta C, was lower than outside the IS. The Kv1.3 variants possessing an intact C-terminus had lower mobility in standalone cells than in IS-engaged cells. This may be related to the observed segregation of F-actin into a ring-like structure at the periphery of the IS, leaving much of the cell almost void of F-actin. Upon depolarizing treatment, mobility of WT and Delta C channels decreased both in standalone and IS-engaged cells, contrary to non-conducting channels, which themselves caused depolarization. Our results support that Kv1.3 is enriched at the IS via its C-terminal region regardless of conductivity, and that depolarization decreases channel mobility. LA - English DB - MTMT ER - TY - JOUR AU - Kenesei, Ádám AU - Volkó, Julianna AU - Szalóki, Nikoletta AU - Mocsár, Gábor AU - Jambrovics, Károly AU - Balajthy, Zoltán AU - Bodnár, Andrea AU - Tóth, Katalin AU - Waldmann, Thomas A. AU - Vámosi, György TI - IL-15 Trans-Presentation Is an Autonomous, Antigen-Independent Process JF - JOURNAL OF IMMUNOLOGY J2 - J IMMUNOL VL - 207 PY - 2021 IS - 10 SP - 2489 EP - 2500 PG - 12 SN - 0022-1767 DO - 10.4049/jimmunol.2100277 UR - https://m2.mtmt.hu/api/publication/32643972 ID - 32643972 AB - IL-15 plays a pivotal role in the long-term survival of T cells and immunological memory. Its receptor consists of three subunits (IL-15R alpha, IL-2/15R beta, and gamma(c)). IL-15 functions mainly via trans-presentation (TP), during which an APC expressing IL-15 bound to IL-15R alpha presents the ligand to the beta gamma(c) receptor-heterodimer on a neighboring T/NK cell. To date, no direct biophysical evidence for the intercellular assembly of the IL-15R heterotrimer exists. Ag presentation (AP), the initial step of T cell activation, is also based on APC-T cell interaction. We were compelled to ask whether AP has any effect on IL-15 TP or whether they are independent processes. In our human Raji B cell-Jurkat T cell model system, we monitored inter-/intracellular protein interactions upon formation of IL-15 TP and AP receptor complexes by Forster resonance energy transfer measurements. We detected enrichment of IL-15R alpha and IL-2/15R beta at the synapse and positive Forster resonance energy transfer efficiency if Raji cells were pretreated with IL-15, giving direct biophysical evidence for IL-15 TP. IL-15Ra and MHC class II interacted and translocated jointly to the immunological synapse when either ligand was present, whereas IL-2/15Rb and CD3 moved independently of each other. IL-15 TP initiated STAT5 phosphorylation in Jurkat cells, which was not further enhanced by AP. Conversely, IL-15 treatment slightly attenuated Ag-induced phosphorylation of the CD3 zeta chain. Our studies prove that in our model system, IL-15 TP and AP can occur independently, and although AP enhances IL-15R assembly, it has no significant effect on IL-15 signaling during TP. Thus, IL-15 TP can be considered an autonomous, Ag-independent process. LA - English DB - MTMT ER - TY - JOUR AU - Csomós, István AU - Nagy, Péter AU - Filep, Csenge Boróka AU - Rebenku, István AU - Nizsalóczki, Enikő AU - Kovács, Tamás AU - Vámosi, György AU - Mátyus, László AU - Bodnár, Andrea TI - Opposing Effects of Chelidonine on Tyrosine and Serine Phosphorylation of STAT3 in Human Uveal Melanoma Cells JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 22 PY - 2021 IS - 23 SN - 1661-6596 DO - 10.3390/ijms222312974 UR - https://m2.mtmt.hu/api/publication/32523431 ID - 32523431 LA - English DB - MTMT ER - TY - JOUR AU - Vámosi, György AU - Miller, Sarah AU - Sinha, Molika AU - Mocsár, Gábor AU - Renz, Malte TI - Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2021 IS - 170 SP - 1 EP - 15 PG - 15 SN - 1940-087X DO - 10.3791/62241 UR - https://m2.mtmt.hu/api/publication/32064566 ID - 32064566 N1 - Funding Agency and Grant Number: Stanford Cancer Institute; Gynecologic Oncology Division Stanford; National Research, Development and Innovation Office, Hungary [GINOP-2.3.2-15-2016-00026, GINOP-2.3.3-15-2016-00030, NN129371, ANN135107] Funding text: We would like to thank the Neuroscience Imaging Service at Stanford University School of Medicine for providing equipment and space for this project. This research was supported by intramural funding of the Stanford Cancer Institute and the Gynecologic Oncology Division Stanford as well as GINOP-2.3.2-15-2016-00026, GINOP-2.3.3-15-2016-00030, NN129371, ANN135107 from the National Research, Development and Innovation Office, Hungary. LA - English DB - MTMT ER -