@article{MTMT:34352113, title = {Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission (Apr, 10.3791/62241, 2021)}, url = {https://m2.mtmt.hu/api/publication/34352113}, author = {Vámosi, György and Miller, Sarah and Sinha, Molika and Fernandez, Maria Kristha and Mocsár, Gábor and Renz, Malte}, doi = {10.3791/6546}, journal-iso = {JOVE-J VIS EXP}, journal = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, unique-id = {34352113}, issn = {1940-087X}, year = {2023}, eissn = {1940-087X}, pages = {1-3} } @misc{MTMT:34191569, title = {EPIGENETIC MODULATION VIA THE C-TERMINAL TAIL OF H2A.Z}, url = {https://m2.mtmt.hu/api/publication/34191569}, author = {Imre, László and Nánási, Péter Pál and Ibtissem, Benhamza and Kata, Nóra Enyedi and Mocsár, Gábor and Bosire, Rosevalentine and Hegedüs, Éva and Firouzi Niaki, Erfaneh and Csóti, Ágota and Zsuzsanna, Darula and Éva, Csősz and Szilárd, Póliska and Beáta, Scholtz and Gábor, Mező and Bacsó, Zsolt and T. Marc Timmers, H. and Masayuki, Kusakabe and Margit, Balázs and Vámosi, György and Juan, Ausio and Peter, Cheung and Katalin, Tóth and David, Tremethick and Masahiko, Harata and Szabó, Gábor}, unique-id = {34191569}, abstract = {H2A.Z-nucleosomes are present in both euchromatin and heterochromatin and it has proven difficult to interpret their disparate roles in the context of their stability features. Using an in situ assay of nucleosome stability and DT40 cells expressing engineered forms of the histone variant we show that native H2A.Z, but not C-terminally truncated H2A.Z (H2A.ZΔC), is released from nucleosomes of peripheral heterochromatin at unusually high salt concentrations. H2A.Z and H3K9me3 landscapes are reorganized in H2A.ZΔC-nuclei and overall sensitivity of chromatin to nucleases is increased. These tail-dependent differences are recapitulated upon treatment of HeLa nuclei with the H2A.Z-tail-peptide (C9), with MNase sensitivity being increased at specific regions including promoters. Introduced into live cells C9 elicits down-regulation of ∼560 genes with nonrandom chromosomal band-localization and pathway-spectrum. Thus, tail-dependent heterogeneity of H2A.Z-nucleosomes is revealed at all organization levels of chromatin and epigenetic modulation can be achieved by targeting molecular interactions involving its C-terminal tail.Competing Interest StatementThe authors have declared no competing interest.}, year = {2023}, pages = {2021.02.22.432230} } @article{MTMT:34154220, title = {Effective transport of aggregated hypericin encapsulated in SBA-15 nanoporous silica particles for photodynamic therapy of cancer cells}, url = {https://m2.mtmt.hu/api/publication/34154220}, author = {Pevná, Viktória and Zauška, Ľuboš and BENZIANE, ANASS and Vámosi, György and Girman, Vladimír and Miklóšová, Monika and Zeleňák, Vladimír and Huntošová, Veronika and Almáši, Miroslav}, doi = {10.1016/j.jphotobiol.2023.112785}, journal-iso = {J PHOTOCH PHOTOBIO B}, journal = {JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY}, volume = {247}, unique-id = {34154220}, issn = {1011-1344}, year = {2023}, eissn = {1873-2682} } @article{MTMT:33614780, title = {Agonist-controlled competition of RAR and VDR nuclear receptors for heterodimerization with RXR is manifested in their DNA-binding}, url = {https://m2.mtmt.hu/api/publication/33614780}, author = {Rehó, Bálint and FADEL, LINA and Brazda, Peter and BENZIANE, ANASS and Hegedüs, Éva and Sen, Pialy and Gadella, Theodorus W.J. and Tóth, Katalin and Nagy, László and Vámosi, György}, doi = {10.1016/j.jbc.2023.102896}, journal-iso = {J BIOL CHEM}, journal = {JOURNAL OF BIOLOGICAL CHEMISTRY}, volume = {299}, unique-id = {33614780}, issn = {0021-9258}, abstract = {We found previously that nuclear receptors (NRs) compete for heterodimerization with their common partner, retinoid X receptor (RXR), in a ligand-dependent manner. To investigate potential competition in their DNA-binding, we monitored the mobility of retinoic acid receptor (RAR) and vitamin D receptor (VDR) in live cells by fluorescence correlation spectroscopy. First, specific agonist treatment and RXR co-expression additively increased RAR DNA-binding, while both agonist and RXR were required for increased VDR DNA-binding, indicating weaker DNA-binding of the VDR/RXR dimer. Second, co-expression of RAR, VDR, and RXR resulted in competition for DNA-binding. Without ligand, VDR reduced the DNA-bound fraction of RAR and vice versa, i.e., a fraction of RXR molecules was occupied by the competing partner. The DNA-bound fraction of either RAR or VDR was enhanced by its own and diminished by the competing NR's agonist. When treated with both ligands, the DNA-bound fraction of RAR increased as much as due to its own agonist, whereas that of VDR increased less. RXR agonist also increased DNA-binding of RAR at the expense of VDR. In summary, competition between RAR and VDR for RXR is also manifested in their DNA-binding in an agonist-dependent manner: RAR dominates over VDR in the absence of agonist or with both agonists present. Thus, side-effects of NR-ligand-based (retinoids, thiazolidinediones) therapies may be ameliorated by other NR ligands and be at least partly explained by reduced DNA-binding due to competition. Our results also complement the model of NR action by involving competition both for RXR and for DNA-sites.}, year = {2023}, eissn = {1083-351X}, orcid-numbers = {Brazda, Peter/0000-0002-0215-1692; Tóth, Katalin/0000-0002-0081-4720} } @article{MTMT:33434625, title = {Concentration-Dependent Antibacterial Activity of Chitosan on Lactobacillus plantarum}, url = {https://m2.mtmt.hu/api/publication/33434625}, author = {Kovács, Renátó and Erdélyi, Lóránd and Fenyvesi, Ferenc and Balla , Noémi and Kovács, Fruzsina and Vámosi, György and Klusóczki, Ágnes and Gyöngyösi, Alexandra and Bácskay, Ildikó and Vecsernyés, Miklós and Váradi, Judit}, doi = {10.3390/pharmaceutics15010018}, journal-iso = {PHARMACEUTICS}, journal = {PHARMACEUTICS}, volume = {15}, unique-id = {33434625}, issn = {1999-4923}, abstract = {The antimicrobial effect of chitosan and synthetic chitosan derivatives has been confirmed on many Gram-positive and Gram-negative bacteria and fungi. The tests were carried out on pathogenic microorganisms, so the mechanism and concentration dependence of the inhibitory effect of chitosan were revealed. We conducted our tests on a probiotic strain, Lactobacillus plantarum. Commercially available chitosan derivatives of different molecular weights were added to L. plantarum suspension in increasing concentrations. The minimum inhibitory concentration (MIC) value of chitosan was determined and confirmed the viability decreasing effect at concentrations above the MIC with a time-kill assay. The release of bacterium cell content was measured at 260 nm after treatment with 0.001–0.1% concentration chitosan solution. An increase in the permeability of the cell membrane was observed only with the 0.1% treatment. The interaction was also investigated by zeta potential measurement, and the irreversible interaction and concentration dependence were established in all concentrations. The interaction of fluorescein isothiocyanate (FITC) labeled low molecular weight chitosan and bacterial cells labeled with membrane dye (FM® 4–64) was confirmed by confocal microscopy. In conclusion, the inhibitory effect of chitosan was verified on a probiotic strain, which is an undesirable effect in probiotic preparations containing chitosan additives, while the inhibitory effect experienced with pathogenic strains is beneficial.}, year = {2023}, eissn = {1999-4923}, orcid-numbers = {Kovács, Renátó/0000-0003-3946-2424} } @article{MTMT:32832222, title = {Doxorubicin impacts chromatin binding of HMGB1, Histone H1 and retinoic acid receptor}, url = {https://m2.mtmt.hu/api/publication/32832222}, author = {Bosire, Rosevalentine and FADEL, LINA and Mocsár, Gábor and Nánási, Péter Pál and Sen, Pialy and Sharma, Anshu Kumar and Naseem, Muhammad Umair and Kovács, Attila and Kugel, Jennifer and Kroemer, Guido and Vámosi, György and Szabó, Gábor}, doi = {10.1038/s41598-022-11994-z}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {12}, unique-id = {32832222}, issn = {2045-2322}, year = {2022}, eissn = {2045-2322} } @article{MTMT:32776876, title = {Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells}, url = {https://m2.mtmt.hu/api/publication/32776876}, author = {Sebestyén, Veronika and Nagy, Éva and Mocsár, Gábor and Volkó, Julianna and Szilágyi, Orsolya and Kenesei, Ádám and Panyi, György and Tóth, Katalin and Hajdu, Péter Béla and Vámosi, György}, doi = {10.3390/ijms23063313}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {23}, unique-id = {32776876}, issn = {1661-6596}, abstract = {Voltage-gated Kv1.3 potassium channels are essential for maintaining negative membrane potential during T-cell activation. They interact with membrane-associated guanylate kinases (MAGUK-s) via their C-terminus and with TCR/CD3, leading to enrichment at the immunological synapse (IS). Molecular interactions and mobility may impact each other and the function of these proteins. We aimed to identify molecular determinants of Kv1.3 mobility, applying fluorescence correlation spectroscopy on human Jurkat T-cells expressing WT, C-terminally truncated (Delta C), and non-conducting mutants of mGFP-Kv1.3. Delta C cannot interact with MAGUK-s and is not enriched at the IS, whereas cells expressing the non-conducting mutant are depolarized. Here, we found that in standalone cells, mobility of Delta C increased relative to the WT, likely due to abrogation of interactions, whereas mobility of the non-conducting mutant decreased, similar to our previous observations on other membrane proteins in depolarized cells. At the IS formed with Raji B-cells, mobility of WT and non-conducting channels, unlike Delta C, was lower than outside the IS. The Kv1.3 variants possessing an intact C-terminus had lower mobility in standalone cells than in IS-engaged cells. This may be related to the observed segregation of F-actin into a ring-like structure at the periphery of the IS, leaving much of the cell almost void of F-actin. Upon depolarizing treatment, mobility of WT and Delta C channels decreased both in standalone and IS-engaged cells, contrary to non-conducting channels, which themselves caused depolarization. Our results support that Kv1.3 is enriched at the IS via its C-terminal region regardless of conductivity, and that depolarization decreases channel mobility.}, year = {2022}, eissn = {1422-0067}, orcid-numbers = {Kenesei, Ádám/0000-0002-3950-7007; Panyi, György/0000-0001-6227-3301} } @article{MTMT:32643972, title = {IL-15 Trans-Presentation Is an Autonomous, Antigen-Independent Process}, url = {https://m2.mtmt.hu/api/publication/32643972}, author = {Kenesei, Ádám and Volkó, Julianna and Szalóki, Nikoletta and Mocsár, Gábor and Jambrovics, Károly and Balajthy, Zoltán and Bodnár, Andrea and Tóth, Katalin and Waldmann, Thomas A. and Vámosi, György}, doi = {10.4049/jimmunol.2100277}, journal-iso = {J IMMUNOL}, journal = {JOURNAL OF IMMUNOLOGY}, volume = {207}, unique-id = {32643972}, issn = {0022-1767}, abstract = {IL-15 plays a pivotal role in the long-term survival of T cells and immunological memory. Its receptor consists of three subunits (IL-15R alpha, IL-2/15R beta, and gamma(c)). IL-15 functions mainly via trans-presentation (TP), during which an APC expressing IL-15 bound to IL-15R alpha presents the ligand to the beta gamma(c) receptor-heterodimer on a neighboring T/NK cell. To date, no direct biophysical evidence for the intercellular assembly of the IL-15R heterotrimer exists. Ag presentation (AP), the initial step of T cell activation, is also based on APC-T cell interaction. We were compelled to ask whether AP has any effect on IL-15 TP or whether they are independent processes. In our human Raji B cell-Jurkat T cell model system, we monitored inter-/intracellular protein interactions upon formation of IL-15 TP and AP receptor complexes by Forster resonance energy transfer measurements. We detected enrichment of IL-15R alpha and IL-2/15R beta at the synapse and positive Forster resonance energy transfer efficiency if Raji cells were pretreated with IL-15, giving direct biophysical evidence for IL-15 TP. IL-15Ra and MHC class II interacted and translocated jointly to the immunological synapse when either ligand was present, whereas IL-2/15Rb and CD3 moved independently of each other. IL-15 TP initiated STAT5 phosphorylation in Jurkat cells, which was not further enhanced by AP. Conversely, IL-15 treatment slightly attenuated Ag-induced phosphorylation of the CD3 zeta chain. Our studies prove that in our model system, IL-15 TP and AP can occur independently, and although AP enhances IL-15R assembly, it has no significant effect on IL-15 signaling during TP. Thus, IL-15 TP can be considered an autonomous, Ag-independent process.}, year = {2021}, eissn = {1550-6606}, pages = {2489-2500}, orcid-numbers = {Kenesei, Ádám/0000-0002-3950-7007} } @article{MTMT:32523431, title = {Opposing Effects of Chelidonine on Tyrosine and Serine Phosphorylation of STAT3 in Human Uveal Melanoma Cells}, url = {https://m2.mtmt.hu/api/publication/32523431}, author = {Csomós, István and Nagy, Péter and Filep, Csenge Boróka and Rebenku, István and Nizsalóczki, Enikő and Kovács, Tamás and Vámosi, György and Mátyus, László and Bodnár, Andrea}, doi = {10.3390/ijms222312974}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {22}, unique-id = {32523431}, issn = {1661-6596}, year = {2021}, eissn = {1422-0067}, orcid-numbers = {Nagy, Péter/0000-0002-7466-805X; Kovács, Tamás/0000-0002-1084-9847; Bodnár, Andrea/0000-0002-9610-4589} } @article{MTMT:32064566, title = {Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission}, url = {https://m2.mtmt.hu/api/publication/32064566}, author = {Vámosi, György and Miller, Sarah and Sinha, Molika and Mocsár, Gábor and Renz, Malte}, doi = {10.3791/62241}, journal-iso = {JOVE-J VIS EXP}, journal = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS}, unique-id = {32064566}, issn = {1940-087X}, year = {2021}, eissn = {1940-087X}, pages = {1-15} }