TY - JOUR AU - Szeles, Zsolt AU - Petheő, Gábor L. AU - Szikora, Bence AU - Kacskovics, Imre AU - Geiszt, Miklós TI - A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-44018-5 UR - https://m2.mtmt.hu/api/publication/34198665 ID - 34198665 AB - Members of the NOX/DUOX family of NADPH oxidases are responsible for regulated ROS production in diverse cells and tissues. Detection of NOX/DUOX proteins at the protein level remains an important challenge in the field. Here we report the development and characterization of a novel anti-NOX5 monoclonal antibody, which recognizes the human NOX5 protein in both Western blot, immunocytochemistry, and histochemistry applications. With the help of the antibody we could successfully detect both heterologously and endogenously expressed NOX5 in mammalian cells. Furthermore, we could also detect NOX5 protein in the human spleen, testis, and ovary. Immunohistochemical studies on human testis revealed that NOX5 localized to spermatogenic cells. This expression pattern was also supported by the result of in silico analysis of single-cell RNA sequencing data that indicated that NOX5 protein is present in developing spermatids and spermatocytes. Mature spermatozoa, however, did not contain detectable NOX5. In the human ovary, both immunostaining and single-cell RNA sequencing suggest that NOX5 is expressed in interstitial fibroblasts and theca cells. We also analyzed vascular cells for the presence of NOX5 and we found that NOX5 expression is a fairly specific feature of splenic endothelial cells. LA - English DB - MTMT ER - TY - JOUR AU - Marx, Anita AU - Osváth, Magdolna AU - Szikora, Bence AU - Pipek, Orsolya Anna AU - Csabai, István AU - Nagy, Ákos AU - Bödör, Csaba AU - Matula, Zsolt AU - Nagy, Ginette AU - Bors, András AU - Uher, Ferenc AU - Mikala, Gábor AU - Vályi-Nagy, István AU - Kacskovics, Imre TI - Liquid biopsy-based monitoring of residual disease in multiple myeloma by analysis of the rearranged immunoglobulin genes–A feasibility study JF - PLOS ONE J2 - PLOS ONE VL - 18 PY - 2023 IS - 5 PG - 23 SN - 1932-6203 DO - 10.1371/journal.pone.0285696 UR - https://m2.mtmt.hu/api/publication/33893006 ID - 33893006 N1 - * Megosztott szerzőség AB - The need for sensitive monitoring of minimal/measurable residual disease (MRD) in multiple myeloma emerged as novel therapies led to deeper responses. Moreover, the potential benefits of blood-based analyses, the so-called liquid biopsy is prompting more and more studies to assess its feasibility. Considering these recent demands, we aimed to optimize a highly sensitive molecular system based on the rearranged immunoglobulin (Ig) genes to monitor MRD from peripheral blood. We analyzed a small group of myeloma patients with the high-risk t(4;14) translocation, using next-generation sequencing of Ig genes and droplet digital PCR of patient-specific Ig heavy chain (IgH) sequences. Moreover, well established monitoring methods such as multiparametric flow cytometry and RT-qPCR of the fusion transcript IgH :: MMSET (IgH and multiple myeloma SET domain-containing protein) were utilized to evaluate the feasibility of these novel molecular tools. Serum measurements of M-protein and free light chains together with the clinical assessment by the treating physician served as routine clinical data. We found significant correlation between our molecular data and clinical parameters, using Spearman correlations. While the comparisons of the Ig-based methods and the other monitoring methods (flow cytometry, qPCR) were not statistically evaluable, we found common trends in their target detection. Regarding longitudinal disease monitoring, the applied methods yielded complementary information thus increasing the reliability of MRD evaluation. We also detected indications of early relapse before clinical signs, although this implication needs further verification in a larger patient cohort. LA - English DB - MTMT ER - TY - JOUR AU - Pape, Veronika AU - Kovács, Hajnal Anna AU - Szatmári, István AU - Ugrai, Imre AU - Szikora, Bence AU - Kacskovics, Imre AU - May, Zoltán AU - Szoboszlai, Norbert AU - Sirokmány, Gábor AU - Geiszt, Miklós TI - Measuring peroxidasin activity in live cells using bromide addition for signal amplification JF - REDOX BIOLOGY J2 - REDOX BIOL VL - 54 PY - 2022 PG - 14 SN - 2213-2317 DO - 10.1016/j.redox.2022.102385 UR - https://m2.mtmt.hu/api/publication/32918558 ID - 32918558 N1 - Department of Physiology, Semmelweis University, Faculty of Medicine, Tűzoltó utca 37-47, Budapest, H-1094, Hungary Institute of Pharmaceutical Chemistry and Stereochemistry Research Group of Hungarian Academy of Sciences, University of Szeged, Eötvös u. 6, Szeged, H-6720, Hungary ImmunoGenes Ltd., Budakeszi, Hungary Research Centre for Natural Sciences, Eötvös Loránd Research Network, Magyar Tudósok körútja 2, Budapest, H-1117, Hungary Institute of Chemistry, Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, H-1117, Hungary Export Date: 20 July 2022 Correspondence Address: Geiszt, M.; Department of Physiology, Tűzoltó utca 37-47, Hungary; email: geiszt.miklos@med.semmelweis-univ.hu Correspondence Address: Pape, V.F.S.; Department of Physiology, Tűzoltó utca 37-47, Hungary; email: veronika.pape@med.semmelweis-univ.hu LA - English DB - MTMT ER - TY - JOUR AU - Kacskovics, Imre TI - Monoklonális antitestek és egyéb biológiai terápiák a COVID–19 kezelésére JF - SCIENTIA ET SECURITAS J2 - SCI SEC VL - 2 PY - 2021 IS - 2 SP - 200 EP - 206 PG - 7 SN - 2732-2688 DO - 10.1556/112.2021.00047 UR - https://m2.mtmt.hu/api/publication/32369543 ID - 32369543 AB - A SARS-CoV-2 koronavírus által kiváltott pandémia az elmúlt mintegy 100 év legsúlyosabb közegészségügyi, gazda-sági és társadalmi válságát okozza. Az emberiség soha nem látott tudással, példa nélküli sebességgel állította elő azokat a hatékony védőoltásokat, amelyek a megfelelő átoltottság mellett kontrollálhatják a COVID–19-járványt.A SARS-CoV-2 fertőzéssel az emberiségnek meg kell tanulnia együtt élni, és mivel a vakcinák nem mindenkinek adhatók, vagy nem mindenkiben váltanak ki immunvédelmet, szükséges a jelenleginél hatékonyabb COVID–19-spe-cifikus terápiák kifejlesztése. Több COVID–19 kezelésére kifejlesztett gyógyszerhatóanyagot is sikerrel tesztelnek, közülük is kiemelkednek a monoklonális antitestek, illetve más biológiai terápiák. Ezek egyfelől olyan gyógyszerek, amelyek a betegség korai fázisában semlegesítik a vírust, azaz megakadályozzák, hogy a sejteket megfertőzze, míg mások a már kialakult súlyos megbetegedésben csökkenthetik a gyulladás mértékét. Biologikumok közé tartozik a SARS-CoV-2-t semlegesítő hACE2-Fc fúziós fehérje is, amely a neutralizáló monoklonális antitestek hatásához hasonlítható; előnye, hogy minden mutáns ellen hatékony lehet.Virológusok, járványügyi szakemberek szerint fel kell készülnünk arra, hogy a jelenlegihez hasonló járványok rendszeressé válnak. Ökológiai okok miatt egyre nő az állati eredetű kórokozók adaptálódása az emberhez, de nem zárhatók ki az ún. laborszökevény vírusok, sőt a bioterrorizmus veszélye sem. Mindezek hatékony kezelésére erősítenünk kell a hazai biotechnológiai kapacitásokat. A hatóanyagfejlesztésben a már kialakult hazai egyetemi-kutatóintézeti-ipari együttműködésekre számíthatunk, a gyártás során pedig a hazai korszerű biotechnológiai, gyógyszeripari kapacitásra, amelyek növelhetik az önellátásból származó biztonságot. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Petheő, Gábor L. AU - Kerekes, Andrea AU - Mihálffy, Máté AU - Donkó, Ágnes AU - Bodrogi, Lilla AU - Skoda, Gabriella AU - Baráth, M. AU - Hoffmann, Orsolya Ivett AU - Szeles, Zsolt AU - Balázs, Bernadett AU - Sirokmány, Gábor AU - Fábián, J.R. AU - Tóth, Zsuzsanna AU - Baksa, I. AU - Kacskovics, Imre AU - Hunyady, László AU - Hiripi, László AU - Bősze, Zsuzsanna AU - Geiszt, Miklós TI - Disruption of the NOX5 Gene Aggravates Atherosclerosis in Rabbits JF - CIRCULATION RESEARCH J2 - CIRC RES VL - 128 PY - 2021 IS - 9 SP - 1320 EP - 1322 PG - 3 SN - 0009-7330 DO - 10.1161/CIRCRESAHA.120.318611 UR - https://m2.mtmt.hu/api/publication/32021120 ID - 32021120 N1 - Funding Agency and Grant Number: National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [K119955, K133002, K115422, FK124708, PD103960, NVKP_16-1-2016-0039, 2020-4.1.1.-TKP2020]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences; [VE-KOP-2.3.2-16-2016-00002] Funding text: This work was supported by grants from the National Research, Development and Innovation Office (K119955, K133002, K115422, FK124708, PD103960, NVKP_16-1-2016-0039, 2020-4.1.1.-TKP2020) and by grant VE-KOP-2.3.2-16-2016-00002 and by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. LA - English DB - MTMT ER - TY - JOUR AU - Kozák, Eszter Zita AU - Szikora, Bence AU - Iliás, Attila AU - Jani, Péter Károly AU - Hegyi, Zoltán AU - Matula, Zsolt AU - Dedinszki, Dóra AU - Tökési, Natália AU - Fülöp, Krisztina AU - Pomozi, Viola AU - Várady, György AU - Bakos, Éva AU - Tusnády, Gábor AU - Kacskovics, Imre AU - Váradi, András TI - Creation of the first monoclonal antibody recognizing an extracellular epitope of hABCC6. JF - FEBS LETTERS J2 - FEBS LETT VL - 595 PY - 2021 IS - 6 SP - 789 EP - 798 PG - 10 SN - 0014-5793 DO - 10.1002/1873-3468.13991 UR - https://m2.mtmt.hu/api/publication/31655245 ID - 31655245 N1 - Imre Kacskovics and Andras Váradi shared senior authorship. AB - Mutations in the ABCC6 gene result in calcification diseases such as pseudoxanthoma elasticum or Generalized Arterial Calcification of Infancy. Generation of antibodies recognizing an extracellular epitope of ABCC6 has been hampered by the short extracellular segments of the protein. To overcome this limitation, we immunized Bovine FcRn transgenic mice exhibiting an augmented humoral immune response with HEK cells expressing human ABCC6 (hABCC6). We obtained a monoclonal antibody recognizing an extracellular epitope of hABCC6 that we named mEChC6. Limited proteolysis revealed that the epitope is within a loop in the N-terminal half of ABCC6 and probably spans amino acids 338-347. mEChC6 recognizes hABCC6 in the liver of hABCC6 transgenic mice, verifying both specificity and extracellular binding to intact hepatocytes. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Ákos AU - Bátai, Bence AU - Kiss, Laura AU - Marx, Anita AU - Pinczés, László Imre AU - Papp, Gergő AU - Kacskovics, Imre AU - Alpár, Donát AU - Bödör, Csaba TI - Folyadékbiopszia-vizsgálatok alkalmazási lehetőségei az onkohematológiában JF - HEMATOLÓGIA-TRANSZFUZIOLÓGIA J2 - HEMATOLÓGIA-TRANSZFUZIOLÓGIA VL - 53 PY - 2020 IS - 3 SP - 144 EP - 156 PG - 13 SN - 1786-5913 DO - 10.1556/2068.2020.53.3.3 UR - https://m2.mtmt.hu/api/publication/31639880 ID - 31639880 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szikora, Bence AU - Marx, Anita AU - Jani, Péter Károly AU - Pipek, Orsolya Anna AU - Müller, Viktor AU - Csabai, István AU - Kacskovics, Imre TI - FcRn Overexpression Expands Diversity of the Humoral Immune Response in bFcRn Transgenic Mice JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 11 PY - 2020 PG - 12 SN - 1664-3224 DO - 10.3389/fimmu.2020.01887 UR - https://m2.mtmt.hu/api/publication/31550862 ID - 31550862 N1 - Department of Immunology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary ImmunoGenes Ltd, Budakeszi, Hungary Department of Physics of Complex Systems, Institute of Physics, Eötvös Loránd University, Budapest, Hungary Department of Plant Systematics, Ecology and Theoretical Biology, Institute of Biology, Eötvös Loránd University, Budapest, Hungary Cited By :2 Export Date: 2 January 2023 Correspondence Address: Kacskovics, I.; Department of Immunology, Hungary; email: imre.kacskovics@ttk.elte.hu Correspondence Address: Kacskovics, I.; ImmunoGenes LtdHungary; email: imre.kacskovics@ttk.elte.hu LA - English DB - MTMT ER - TY - JOUR AU - László, Zsófia Ilona AU - Lele, Zsolt AU - Zöldi, Miklós AU - Csapóné Miczán, Vivien AU - Mógor, Fruzsina AU - Simon, Gabriel M. AU - Mackie, Ken AU - Kacskovics, Imre AU - Cravatt, Benjamin F. AU - Katona, István TI - ABHD4-dependent developmental anoikis safeguards the embryonic brain JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 11 PY - 2020 IS - 1 PG - 16 SN - 2041-1723 DO - 10.1038/s41467-020-18175-4 UR - https://m2.mtmt.hu/api/publication/31523670 ID - 31523670 N1 - Momentum Laboratory of Molecular Neurobiology, Institute of Experimental Medicine, 1450 Budapest Pf. 67., Budapest, Hungary School of Ph.D. Studies, Semmelweis University, Budapest, Hungary Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, Budapest, Hungary The Skaggs Institute for Chemical Biology, Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92307, United States Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN 47405, United States Department of Immunology, Eötvös Loránd University, Pázmány Péter stny 1/A., Budapest, 1117, Hungary ImmunoGenes Ltd, Makkosi út 86., Budakeszi, 2092, Hungary Cited By :11 Export Date: 4 May 2023 Correspondence Address: Katona, I.; Momentum Laboratory of Molecular Neurobiology, 1450 Budapest Pf. 67., Hungary; email: katona@koki.hu Chemicals/CAS: protein, 67254-75-5; lysophospholipase, 9001-85-8; ABHD4 protein, human; Lysophospholipase Funding details: 129961, K116915, VEKOP-2.3.3-15-2016-00013, VKSZ-14-1-2O15-0155 Funding details: National Institutes of Health, NIH, DA011322, DA037660, R01DA044925 Funding details: National Institute on Drug Abuse, NIDA, K05DA021696 Funding details: National Institute of Neurological Disorders and Stroke, NINDS, R01NS099457 Funding details: Semmelweis Egyetem, EFOP-3.6.3-VEKOP-16-2017-00009 Funding text 1: The authors thank Dr. E. Horváth, B. Pintér, E. Tischler for laboratory support, Drs. G. Balogh, Z. Balogi, A. Dorning, B. Dudok, M. Melis, M. Péter, S. Prokop, L. Vígh for help with preliminary experiments and comments. The authors appreciate the help of Dr. Ozge Gunduz-Cinar and Dr. Andrew Holmes with the RNAscope protocol. The authors are grateful to the IEM Medical Genetics Unit for mouse colony management, to S.S.J. Hu, K. Nagy for antibody generation, to Dr. B. Gereben for providing HEK293 cells and to K. Balogh for artwork. The help of Dr. L. Barna, the Nikon Microscopy Center at the Institute of Experimental Medicine, Nikon Europe B.V., Nikon Austria GmbH and Auro-Science Consulting is acknowledged for kindly providing microscopy support. This work was supported by the National Brain Research Program (2017-1.2.1-NKP-2017-00002), by the National Research, Development and Innovation Office, Hungary (VKSZ-14-1-2O15-0155 for antibody generation; VEKOP-2.3.3-15-2016-00013 for super-resolution microscopy development; K116915 for ABHD4 research and Frontier Program 129961 for cannabinoid research). I.K. holds the Naus Family Chair in Addiction Sciences in the Department of Psychological and Brain Sciences at Indiana University Bloomington and his work is also supported by the National Institutes of Health (R01NS099457 and R01DA044925). Additional support was provided by the Semmelweis University Grant (EFOP-3.6.3-VEKOP-16-2017-00009 to Z.I.L.), the US National Institutes of Health Grant (DA021696 and DA011322) to K.M. and (DA037660) to B.F.C. LA - English DB - MTMT ER - TY - JOUR AU - Kaló, Zoltán AU - Vályi-Nagy, István AU - Szekely, A. AU - Szikora, Bence AU - Bendes, R. AU - Varga, G. AU - Szilberhorn, László AU - Nagy, Balázs AU - Mikala, Gábor AU - Kacskovics, Imre TI - EARLY PHASE ECONOMIC EVALUATION OF NEW GENERATION SEQUENCE DIAGNOSTICS IN MULTIPLE MYELOMA JF - VALUE IN HEALTH J2 - VALUE HEALTH VL - 23 PY - 2020 IS - Supplement 1 SP - S326 EP - S327 SN - 1098-3015 DO - 10.1016/j.jval.2020.04.1222 UR - https://m2.mtmt.hu/api/publication/31323384 ID - 31323384 LA - English DB - MTMT ER -