@article{MTMT:34198665,
	title = {A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells},
	url = {https://m2.mtmt.hu/api/publication/34198665},
	author = {Szeles, Zsolt and Petheő, Gábor L. and Szikora, Bence and Kacskovics, Imre and Geiszt, Miklós},
	doi = {10.1038/s41598-023-44018-5},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {34198665},
	issn = {2045-2322},
	abstract = {Members of the NOX/DUOX family of NADPH oxidases are responsible for regulated ROS production in diverse cells and tissues. Detection of NOX/DUOX proteins at the protein level remains an important challenge in the field. Here we report the development and characterization of a novel anti-NOX5 monoclonal antibody, which recognizes the human NOX5 protein in both Western blot, immunocytochemistry, and histochemistry applications. With the help of the antibody we could successfully detect both heterologously and endogenously expressed NOX5 in mammalian cells. Furthermore, we could also detect NOX5 protein in the human spleen, testis, and ovary. Immunohistochemical studies on human testis revealed that NOX5 localized to spermatogenic cells. This expression pattern was also supported by the result of in silico analysis of single-cell RNA sequencing data that indicated that NOX5 protein is present in developing spermatids and spermatocytes. Mature spermatozoa, however, did not contain detectable NOX5. In the human ovary, both immunostaining and single-cell RNA sequencing suggest that NOX5 is expressed in interstitial fibroblasts and theca cells. We also analyzed vascular cells for the presence of NOX5 and we found that NOX5 expression is a fairly specific feature of splenic endothelial cells.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Petheő, Gábor L./0000-0002-1773-4129; Szikora, Bence/0000-0002-0766-5684; Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:33893006,
	title = {Liquid biopsy-based monitoring of residual disease in multiple myeloma by analysis of the rearranged immunoglobulin genes–A feasibility study},
	url = {https://m2.mtmt.hu/api/publication/33893006},
	author = {Marx, Anita and Osváth, Magdolna and Szikora, Bence and Pipek, Orsolya Anna and Csabai, István and Nagy, Ákos and Bödör, Csaba and Matula, Zsolt and Nagy, Ginette and Bors, András and Uher, Ferenc and Mikala, Gábor and Vályi-Nagy, István and Kacskovics, Imre},
	doi = {10.1371/journal.pone.0285696},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {18},
	unique-id = {33893006},
	issn = {1932-6203},
	abstract = {The need for sensitive monitoring of minimal/measurable residual disease (MRD) in multiple myeloma emerged as novel therapies led to deeper responses. Moreover, the potential benefits of blood-based analyses, the so-called liquid biopsy is prompting more and more studies to assess its feasibility. Considering these recent demands, we aimed to optimize a highly sensitive molecular system based on the rearranged immunoglobulin (Ig) genes to monitor MRD from peripheral blood. We analyzed a small group of myeloma patients with the high-risk t(4;14) translocation, using next-generation sequencing of Ig genes and droplet digital PCR of patient-specific Ig heavy chain (IgH) sequences. Moreover, well established monitoring methods such as multiparametric flow cytometry and RT-qPCR of the fusion transcript IgH :: MMSET (IgH and multiple myeloma SET domain-containing protein) were utilized to evaluate the feasibility of these novel molecular tools. Serum measurements of M-protein and free light chains together with the clinical assessment by the treating physician served as routine clinical data. We found significant correlation between our molecular data and clinical parameters, using Spearman correlations. While the comparisons of the Ig-based methods and the other monitoring methods (flow cytometry, qPCR) were not statistically evaluable, we found common trends in their target detection. Regarding longitudinal disease monitoring, the applied methods yielded complementary information thus increasing the reliability of MRD evaluation. We also detected indications of early relapse before clinical signs, although this implication needs further verification in a larger patient cohort.},
	keywords = {multiple myeloma; liquid biopsy; circulating tumor DNA; next-generation sequencing (NGS); Circulating tumor cells; minimal residual disease (MRD); Droplet digital PCR (ddPCR); immunoglobulin genes},
	year = {2023},
	eissn = {1932-6203},
	orcid-numbers = {Marx, Anita/0000-0001-8969-5766; Szikora, Bence/0000-0002-0766-5684; Pipek, Orsolya Anna/0000-0001-8109-0340; Csabai, István/0000-0001-9232-9898; Bödör, Csaba/0000-0002-0729-692X; Bors, András/0000-0003-2109-4678; Uher, Ferenc/0000-0001-7997-6142; Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:32918558,
	title = {Measuring peroxidasin activity in live cells using bromide addition for signal amplification},
	url = {https://m2.mtmt.hu/api/publication/32918558},
	author = {Pape, Veronika and Kovács, Hajnal Anna and Szatmári, István and Ugrai, Imre and Szikora, Bence and Kacskovics, Imre and May, Zoltán and Szoboszlai, Norbert and Sirokmány, Gábor and Geiszt, Miklós},
	doi = {10.1016/j.redox.2022.102385},
	journal-iso = {REDOX BIOL},
	journal = {REDOX BIOLOGY},
	volume = {54},
	unique-id = {32918558},
	issn = {2213-2317},
	year = {2022},
	eissn = {2213-2317},
	orcid-numbers = {Pape, Veronika/0000-0001-6589-6950; Kovács, Hajnal Anna/0000-0001-7928-7531; Szatmári, István/0000-0002-8571-5229; Szikora, Bence/0000-0002-0766-5684; Kacskovics, Imre/0000-0002-0402-3862; Szoboszlai, Norbert/0000-0002-3991-3996; Sirokmány, Gábor/0000-0002-9473-2434}
}

@article{MTMT:32369543,
	title = {Monoklonális antitestek és egyéb biológiai terápiák a COVID–19 kezelésére},
	url = {https://m2.mtmt.hu/api/publication/32369543},
	author = {Kacskovics, Imre},
	doi = {10.1556/112.2021.00047},
	journal-iso = {SCI SEC},
	journal = {SCIENTIA ET SECURITAS},
	volume = {2},
	unique-id = {32369543},
	abstract = {A SARS-CoV-2 koronavírus által kiváltott pandémia az elmúlt mintegy 100 év legsúlyosabb közegészségügyi, gazda-sági  és  társadalmi  válságát  okozza.  Az  emberiség  soha  nem  látott  tudással,  példa  nélküli  sebességgel  állította  elő  azokat a hatékony védőoltásokat, amelyek a megfelelő átoltottság mellett kontrollálhatják a COVID–19-járványt.A SARS-CoV-2 fertőzéssel az emberiségnek meg kell tanulnia együtt élni, és mivel a vakcinák nem mindenkinek adhatók, vagy nem mindenkiben váltanak ki immunvédelmet, szükséges a jelenleginél hatékonyabb COVID–19-spe-cifikus  terápiák  kifejlesztése.  Több  COVID–19  kezelésére  kifejlesztett  gyógyszerhatóanyagot  is  sikerrel  tesztelnek,  közülük is kiemelkednek a monoklonális antitestek, illetve más biológiai terápiák. Ezek egyfelől olyan gyógyszerek, amelyek a betegség korai fázisában semlegesítik a vírust, azaz megakadályozzák, hogy a sejteket megfertőzze, míg mások a már kialakult súlyos megbetegedésben csökkenthetik a gyulladás mértékét. Biologikumok közé tartozik a SARS-CoV-2-t semlegesítő hACE2-Fc fúziós fehérje is, amely a neutralizáló monoklonális antitestek hatásához hasonlítható; előnye, hogy minden mutáns ellen hatékony lehet.Virológusok,  járványügyi  szakemberek  szerint  fel  kell  készülnünk  arra,  hogy  a  jelenlegihez  hasonló  járványok  rendszeressé válnak. Ökológiai okok miatt egyre nő az állati eredetű kórokozók adaptálódása az emberhez, de nem zárhatók ki az ún. laborszökevény vírusok, sőt a bioterrorizmus veszélye sem. Mindezek hatékony kezelésére erősítenünk kell a hazai biotechnológiai kapacitásokat. A hatóanyagfejlesztésben a már kialakult hazai egyetemi-kutatóintézeti-ipari együttműködésekre számíthatunk, a gyártás során pedig a hazai korszerű biotechnológiai, gyógyszeripari kapacitásra, amelyek növelhetik az önellátásból származó biztonságot.},
	keywords = {COVID-19; koronavírus; terápiás monoklonális antitestek; Fc-fúziós fehérjék; biologikumok},
	year = {2021},
	eissn = {2732-2688},
	pages = {200-206},
	orcid-numbers = {Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:32021120,
	title = {Disruption of the NOX5 Gene Aggravates Atherosclerosis in Rabbits},
	url = {https://m2.mtmt.hu/api/publication/32021120},
	author = {Petheő, Gábor L. and Kerekes, Andrea and Mihálffy, Máté and Donkó, Ágnes and Bodrogi, Lilla and Skoda, Gabriella and Baráth, M. and Hoffmann, Orsolya Ivett and Szeles, Zsolt and Balázs, Bernadett and Sirokmány, Gábor and Fábián, J.R. and Tóth, Zsuzsanna and Baksa, I. and Kacskovics, Imre and Hunyady, László and Hiripi, László and Bősze, Zsuzsanna and Geiszt, Miklós},
	doi = {10.1161/CIRCRESAHA.120.318611},
	journal-iso = {CIRC RES},
	journal = {CIRCULATION RESEARCH},
	volume = {128},
	unique-id = {32021120},
	issn = {0009-7330},
	keywords = {RABBITS; ATHEROSCLEROSIS; Reactive oxygen species; Blood Vessels; NADPH Oxidase 5},
	year = {2021},
	eissn = {1524-4571},
	pages = {1320-1322},
	orcid-numbers = {Petheő, Gábor L./0000-0002-1773-4129; Bodrogi, Lilla/0000-0003-4696-9282; Balázs, Bernadett/0000-0003-3046-0780; Sirokmány, Gábor/0000-0002-9473-2434; Tóth, Zsuzsanna/0000-0002-0628-1320; Kacskovics, Imre/0000-0002-0402-3862; Hunyady, László/0000-0002-8438-7251}
}

@article{MTMT:31655245,
	title = {Creation of the first monoclonal antibody recognizing an extracellular epitope of hABCC6.},
	url = {https://m2.mtmt.hu/api/publication/31655245},
	author = {Kozák, Eszter Zita and Szikora, Bence and Iliás, Attila and Jani, Péter Károly and Hegyi, Zoltán and Matula, Zsolt and Dedinszki, Dóra and Tökési, Natália and Fülöp, Krisztina and Pomozi, Viola and Várady, György and Bakos, Éva and Tusnády, Gábor and Kacskovics, Imre and Váradi, András},
	doi = {10.1002/1873-3468.13991},
	journal-iso = {FEBS LETT},
	journal = {FEBS LETTERS},
	volume = {595},
	unique-id = {31655245},
	issn = {0014-5793},
	abstract = {Mutations in the ABCC6 gene result in calcification diseases such as pseudoxanthoma elasticum or Generalized Arterial Calcification of Infancy. Generation of antibodies recognizing an extracellular epitope of ABCC6 has been hampered by the short extracellular segments of the protein. To overcome this limitation, we immunized Bovine FcRn transgenic mice exhibiting an augmented humoral immune response with HEK cells expressing human ABCC6 (hABCC6). We obtained a monoclonal antibody recognizing an extracellular epitope of hABCC6 that we named mEChC6. Limited proteolysis revealed that the epitope is within a loop in the N-terminal half of ABCC6 and probably spans amino acids 338-347. mEChC6 recognizes hABCC6 in the liver of hABCC6 transgenic mice, verifying both specificity and extracellular binding to intact hepatocytes.},
	keywords = {monoclonal antibody; Ectopic calcification; ABCC6; extracellular epitope},
	year = {2021},
	eissn = {1873-3468},
	pages = {789-798},
	orcid-numbers = {Szikora, Bence/0000-0002-0766-5684; Iliás, Attila/0000-0003-3196-0795; Várady, György/0000-0003-2012-9680; Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:31639880,
	title = {Folyadékbiopszia-vizsgálatok alkalmazási lehetőségei az onkohematológiában},
	url = {https://m2.mtmt.hu/api/publication/31639880},
	author = {Nagy, Ákos and Bátai, Bence and Kiss, Laura and Marx, Anita and Pinczés, László Imre and Papp, Gergő and Kacskovics, Imre and Alpár, Donát and Bödör, Csaba},
	doi = {10.1556/2068.2020.53.3.3},
	journal-iso = {HEMATOLÓGIA-TRANSZFUZIOLÓGIA},
	journal = {HEMATOLÓGIA-TRANSZFUZIOLÓGIA},
	volume = {53},
	unique-id = {31639880},
	issn = {1786-5913},
	year = {2020},
	pages = {144-156},
	orcid-numbers = {Marx, Anita/0000-0001-8969-5766; Pinczés, László Imre/0000-0003-0453-1709; Papp, Gergő/0000-0001-5840-2369; Kacskovics, Imre/0000-0002-0402-3862; Bödör, Csaba/0000-0002-0729-692X}
}

@article{MTMT:31550862,
	title = {FcRn Overexpression Expands Diversity of the Humoral Immune Response in bFcRn Transgenic Mice},
	url = {https://m2.mtmt.hu/api/publication/31550862},
	author = {Szikora, Bence and Marx, Anita and Jani, Péter Károly and Pipek, Orsolya Anna and Müller, Viktor and Csabai, István and Kacskovics, Imre},
	doi = {10.3389/fimmu.2020.01887},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {11},
	unique-id = {31550862},
	issn = {1664-3224},
	year = {2020},
	eissn = {1664-3224},
	orcid-numbers = {Szikora, Bence/0000-0002-0766-5684; Marx, Anita/0000-0001-8969-5766; Pipek, Orsolya Anna/0000-0001-8109-0340; Müller, Viktor/0000-0001-8212-4880; Csabai, István/0000-0001-9232-9898; Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:31523670,
	title = {ABHD4-dependent developmental anoikis safeguards the embryonic brain},
	url = {https://m2.mtmt.hu/api/publication/31523670},
	author = {László, Zsófia Ilona and Lele, Zsolt and Zöldi, Miklós and Csapóné Miczán, Vivien and Mógor, Fruzsina and Simon, Gabriel M. and Mackie, Ken and Kacskovics, Imre and Cravatt, Benjamin F. and Katona, István},
	doi = {10.1038/s41467-020-18175-4},
	journal-iso = {NAT COMMUN},
	journal = {NATURE COMMUNICATIONS},
	volume = {11},
	unique-id = {31523670},
	issn = {2041-1723},
	year = {2020},
	eissn = {2041-1723},
	orcid-numbers = {Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:31323384,
	title = {EARLY PHASE ECONOMIC EVALUATION OF NEW GENERATION SEQUENCE DIAGNOSTICS IN MULTIPLE MYELOMA},
	url = {https://m2.mtmt.hu/api/publication/31323384},
	author = {Kaló, Zoltán and Vályi-Nagy, István and Szekely, A. and Szikora, Bence and Bendes, R. and Varga, G. and Szilberhorn, László and Nagy, Balázs and Mikala, Gábor and Kacskovics, Imre},
	doi = {10.1016/j.jval.2020.04.1222},
	journal-iso = {VALUE HEALTH},
	journal = {VALUE IN HEALTH},
	volume = {23},
	unique-id = {31323384},
	issn = {1098-3015},
	year = {2020},
	eissn = {1524-4733},
	pages = {S326-S327},
	orcid-numbers = {Kaló, Zoltán/0000-0001-7762-2607; Szikora, Bence/0000-0002-0766-5684; Nagy, Balázs/0000-0003-4125-7905; Kacskovics, Imre/0000-0002-0402-3862}
}