TY - JOUR AU - Biró, Erika AU - Szegedi, István AU - Kiss, Csongor AU - Vargáné Oláh, Anna AU - Dockrell, Mark AU - Price, Robert G. AU - Szabó, Tamás TI - The role of urinary N-acetyl-beta-D-glucosaminidase in early detection of acute kidney injury among pediatric patients with neoplastic disorders in a retrospective study JF - BMC PEDIATRICS J2 - BMC PEDIATR VL - 22 PY - 2022 IS - 1 SN - 1471-2431 DO - 10.1186/s12887-022-03416-w UR - https://m2.mtmt.hu/api/publication/33131836 ID - 33131836 AB - Background: The 1-year cumulative incidence of AKI reportedly is high (52%) in pediatric neoplastic disorders. About half of these events occur within 2 weeks. However, subclinical AKI episodes may remain unrecognized by the conventional creatinine-based approaches. We investigated the diagnostic value of urinary N-acetyl-beta-D-glucosaminidase (uNAG) as an early marker of acute kidney injury (AKI). Methods: In our retrospective study, 33 children with neoplastic disorders were inculded who had serial uNAG tests (at least 5 samples/patient) with a total of 367 uNAG measurements. Renal function was determined by cystatin-C and creatinine based GFR, and relative increase of uNAG index -(uNAG(RI)). We focused on detecting both clinical and subclinical AKI episodes (according to Biomarker-Guided Risk Assessment using pRIFLE criteria and /or elevated uNAG levels) and the incidence of chronic kidney damage. Results: Sixty episodes in 26 patients, with positivity at least in one parameter of kidney panel, were identified during the observation period. We detected 18/60 clinical and 12/60 subclinical renal episodes. In 27/60 episodes only uNAG values was elevated with no therapeutic consequence at presentation. Two patients were detected with decreased initial creatinine levels with 3”silent” AKI. In 13 patients, modest elevation of uNAG persisted suggesting mild, reversible tubular damage, while chronic tubuloglomerular injury occurred in 5 patients. Based on ROC analysis for the occurence of AKI, uNAG(RI) significantly indicated the presence of AKI, the sensitivity and specificity are higher than the changes of -GFR(Creat). Serial uNAG measurements are recommended for the reduction of the great amount of false positive uNAG results, often due to overhydratation. Conclusion: Use of Biomarker-guided Risk Assessment for AKI identified 1.5 x more clinical and subclinical AKI episodes than with creatinine alone in our pediatric cancer patients. Based on the ROC curve for the occurence of AKI, uNAG(RI) has relatively high sensitivity and specificity comparable to changes of GFR(CysC). The advantage of serial uNAG measurements is to decrease the number of false positive results. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Judit AU - Vargáné Oláh, Anna AU - Petercsák, Tamás AU - Kovács, Tamás AU - Kappelmayer, János TI - Detection of haemolysis, a frequent preanalytical problem in the serum of newborns and adults. JF - ELECTRONIC JOURNAL OF THE INTERNATIONAL FEDERATION OF CLINICAL CHEMISTRY AND LABORATORY MEDICINE J2 - E JIFCC VL - 31 PY - 2020 IS - 1 SP - 6 EP - 14 PG - 9 SN - 1650-3414 UR - https://m2.mtmt.hu/api/publication/31892935 ID - 31892935 AB - Preanalytical problems can be more frequent in case of preterm and term newborns as compared to the general patient population. Here we present the leading preanalytical errors in our laboratory, the prevalence of haemolysis and its impact on laboratory test results, and our efforts to improve the diagnostic workup of newborns' samples.Preanalytical quality indicators were analysed in all samples in 2018. The haemolysis index was measured spectrophotometrically in serum samples in the period of 2012-2018, and the ratio of haemolysed samples and the test rejection rates were analysed. The data of newborns and other patients were analysed separately.During the tested year, the leading preanalytical errors were haemolysis in serum samples, inadequate sample identification and clotting of anticoagulated blood regarding all samples or newborns. In this seven-year period the ratio of haemolysed serum samples was 4.00% in all patients and 46.4% in newborns, while the test rejection rates due to haemolysis were 0.57% and 3.71%, respectively. Haemolysis indices were significantly higher in case of newborns than in patients with documented severe intravascular haemolysis which suggests that the major reason of elevated haemolysis indices in newborns was in vitro haemolysis. Accordingly, all C-reactive protein (CRP) results which were rejected by severe haemolysis became reliable after repeating blood sampling.Haemolysis is the leading preanalytical problem not only in newborns but also in the general patient population. Our study highlights the importance of automated assessment of serum indices and continuous monitoring of the preanalytical quality indicators and suggests the need for education and blood collection trainings. LA - English DB - MTMT ER - TY - JOUR AU - Lénárt, Kinga AU - Pap, Attila AU - Pórszász, Róbert AU - Vargáné Oláh, Anna AU - Fésüs, László AU - Mádi, András TI - Transglutaminase 2 Has Metabolic and Vascular Regulatory Functions Revealed by In Vivo Activation of Alpha1-Adrenergic Receptor JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 21 PY - 2020 IS - 11 SN - 1661-6596 DO - 10.3390/ijms21113865 UR - https://m2.mtmt.hu/api/publication/31332668 ID - 31332668 LA - English DB - MTMT ER - TY - JOUR AU - Hajas, Orsolya AU - Bagoly, Zsuzsa AU - Tóth, Noémi Klára AU - Urbancsek, Réka AU - Kiss, Alexandra AU - Kovács, Kitti Bernadett AU - Sarkady, Ferenc AU - Nagy, Attila Csaba AU - Vargáné Oláh, Anna AU - Nagy, László Tibor AU - Clemens, Marcell AU - Csiba, László AU - Csanádi, Zoltán TI - Intracardiac Fibrinolysis and Endothelium Activation Related to Atrial Fibrillation Ablation with Different Techniques JF - CARDIOLOGY RESEARCH AND PRACTICE J2 - CARDIOL RES PRAC VL - 2020 PY - 2020 PG - 8 SN - 2090-0597 DO - 10.1155/2020/1570483 UR - https://m2.mtmt.hu/api/publication/31143304 ID - 31143304 N1 - 281437 LA - English DB - MTMT ER - TY - JOUR AU - Mádi, András AU - Cuaranta-Monroy, I AU - Lénárt, Kinga AU - Pap, Attila AU - Mezei, Zoltán András AU - Kristóf, Endre AU - Vargáné Oláh, Anna AU - Vámosi, György AU - Bacso, Z AU - Bay, Péter AU - Fésüs, László TI - Browning deficiency and low mobilization of fatty acids in gonadal white adipose tissue leads to decreased cold-tolerance of transglutaminase 2 knock-out mice. JF - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS J2 - BBA-MOL CELL BIOL L VL - 1862 PY - 2017 IS - 12 SP - 1575 EP - 1586 PG - 12 SN - 1388-1981 DO - 10.1016/j.bbalip.2017.07.014 UR - https://m2.mtmt.hu/api/publication/3289057 ID - 3289057 AB - During cold-exposure 'beige' adipocytes with increased mitochondrial content are activated in white adipose tissue (WAT). These cells, similarly to brown adipose tissue (BAT), dissipate stored chemical energy in the form of heat with the help of uncoupling protein 1 (UCP1). We investigated the effect of tissue transglutaminase (TG2) ablation on the function of ATs in mice. Although TG2+/+ and TG2-/- mice had the same amount of WAT and BAT, we found that TG2+/+ animals could tolerate acute cold exposure for 4h, whereas TG2-/- mice only for 3h. Both TG2-/- and TG2+/+ animals used up half of the triacylglycerol content of subcutaneous WAT (SCAT) after 3h treatment; however, TG2-/- mice still possessed markedly whiter and higher amount of gonadal WAT (GONAT) as reflected in the larger size of adipocytes and lower free fatty acid levels in serum. Furthermore, lower expression of 'beige' marker genes such as UCP1, TBX1 and TNFRFS9 was observed after cold exposure in GONAT of TG2-/- mice, paralleled with a lower level of UCP1 protein and a decreased mitochondrial content. The detected changes in gene expression of Resistin and Adiponectin did not provoke glucose intolerance in the investigated TG2-/- mice, and TG2 deletion did not influence adrenaline, noradrenaline, glucagon and insulin production. Our data suggest that TG2 has a tissue-specific role in GONAT function and browning, which becomes apparent under acute cold exposure. LA - English DB - MTMT ER - TY - JOUR AU - Vargáné Oláh, Anna AU - Szalka, T AU - Szantha, E AU - Varga, Éva AU - Mátyus, János AU - Varga, József TI - High Sensitive Troponin T has an Incremental Value in Estimation of Cardiovascular Risk in Chronic Kidney Disease. JF - JOURNAL OF NEPHROLOGY AND THERAPEUTICS J2 - J NEPHROL THERAP VL - 3 PY - 2017 IS - 1 SP - 1 EP - 6 PG - 6 SN - 2161-0959 UR - https://m2.mtmt.hu/api/publication/3188640 ID - 3188640 LA - English DB - MTMT ER - TY - JOUR AU - Mikó, Irén AU - Németh, Norbert AU - Somogyi, Viktória AU - Kiss, F AU - Toth, E AU - Pető, Katalin AU - Furka, Andrea AU - Ványolos, Erzsébet AU - Tóth, László József AU - Varga, József AU - Szigeti, Krisztián AU - Benkő, Ilona AU - Vargáné Oláh, Anna AU - Furka, István TI - Comparative erythrocyte deformability investigations by filtrometry, slit-flow and rotational ektacytometry in a long-term follow-up animal study on splenectomy and different spleen preserving operative techniques. Partial or subtotal spleen resection and spleen autotransplantation TS - Partial or subtotal spleen resection and spleen autotransplantation JF - CLINICAL HEMORHEOLOGY AND MICROCIRCULATION J2 - CLIN HEMORHEOL MICRO VL - 66 PY - 2017 IS - 1 SP - 83 EP - 96 PG - 14 SN - 1386-0291 DO - 10.3233/CH-160231 UR - https://m2.mtmt.hu/api/publication/3178666 ID - 3178666 LA - English DB - MTMT ER - TY - CHAP AU - Vargáné Oláh, Anna AU - Bhattoa Harjit, Pál TI - Autovalidation in laboratory medicine, clinical chemistry. Register of laboratory examinations TS - Register of laboratory examinations T2 - Az élettudományi - klinikai felsőoktatás gyakorlatorientált és hallgatóbarát korszerűsítése a vidéki képzőhelyek nemzetközi versenyképességének erősítésére. PB - Debreceni Egyetem CY - Debrecen PY - 2015 SP - 1 EP - 31 PG - 31 UR - https://m2.mtmt.hu/api/publication/3194060 ID - 3194060 LA - English DB - MTMT ER - TY - JOUR AU - Mikó, Irén AU - Tóth, E AU - Kiss, F AU - Furka, István AU - Pető, Katalin AU - Furka, Andrea AU - Deák, Ádám AU - Ványolos, Erzsébet AU - Vargáné Oláh, Anna AU - Tóth, L AU - Benkő, I AU - Németh, Norbert TI - Change of leukocyte antisedimentation rate and other hematological and hemorheological parameters in beagle dogs with splenectomy and different amount of residual spleen after the mandatory vaccination JF - EUROPEAN SURGICAL RESEARCH J2 - EUR SURG RES VL - 55 PY - 2015 IS - Suppl.1 SP - 158 EP - 159 PG - 2 SN - 0014-312X DO - 10.1159/000381839 UR - https://m2.mtmt.hu/api/publication/3069497 ID - 3069497 LA - English DB - MTMT ER - TY - JOUR AU - Vargáné Oláh, Anna AU - P, Szabó Gabriella AU - Varga, József AU - Koczok, Katalin AU - Balogh, István AU - Harangi, János TI - A veleszületett koleszterinhiány jellemző biomarkerei Smith-Lemli-opitz-szindrómás betegekben és hordozókban JF - METABOLIZMUS J2 - METABOLIZMUS VL - 13 PY - 2015 IS - 3 SP - 196 EP - 201 PG - 6 SN - 1589-7311 UR - https://m2.mtmt.hu/api/publication/3029072 ID - 3029072 LA - Hungarian DB - MTMT ER -