TY - JOUR AU - Búzás, András AU - Makai, András AU - Groma, Géza AU - Dancsházy, Zsolt AU - Szendi, István AU - Kish, Laszlo B. AU - Santa Maria, Anaraquel AU - Dér, András TI - Hierarchical organization of human physical activity JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-024-56185-0 UR - https://m2.mtmt.hu/api/publication/34737118 ID - 34737118 N1 - Institute of Biophysics, HUN-REN Biological Research Centre, Temesvári Krt. 62, Szeged, 6701, Hungary Department of Psychiatry, Kiskunhalas Semmelweis Hospital, 1 Dr. Monszpart László Street, Kiskunhalas, 6400, Hungary Department of Electrical and Computer Engineering, Texas A & amp;M University, TAMUS 3128, College Station, TX 77843-3128, United States Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, United States Export Date: 19 April 2024 Correspondence Address: Santa-Maria, A.R.; Institute of Biophysics, Temesvári Krt. 62, Hungary; email: anaraquel.santamaria@wyss.harvard.edu Correspondence Address: Dér, A.; Institute of Biophysics, Temesvári Krt. 62, Hungary; email: der.andras@brc.hu AB - Human physical activity (HPA), a fundamental physiological signal characteristic of bodily motion is of rapidly growing interest in multidisciplinary research. Here we report the existence of hitherto unidentified hierarchical levels in the temporal organization of HPA on the ultradian scale: on the minute's scale, passive periods are followed by activity bursts of similar intensity (‘quanta’) that are organized into superstructures on the hours- and on the daily scale. The time course of HPA can be considered a stochastic, quasi-binary process, where quanta, assigned to task-oriented actions are organized into work packages on higher levels of hierarchy. In order to grasp the essence of this complex dynamic behaviour, we established a stochastic mathematical model which could reproduce the main statistical features of real activity time series. The results are expected to provide important data for developing novel behavioural models and advancing the diagnostics of neurological or psychiatric diseases. LA - English DB - MTMT ER - TY - JOUR AU - Laczkó-Dobos, Hajnalka AU - Bhattacharjee, Arindam AU - Maddali, Asha Kiran AU - Kincses, András AU - Abuammar, Hussein AU - Sebőkné Nagy, Krisztina AU - Páli, Tibor AU - Dér, András AU - Hegedűs, Tamás AU - Csordás, Gábor AU - Juhász, Gábor TI - PtdIns4p is required for the autophagosomal recruitment of STX17 (syntaxin 17) to promote lysosomal fusion JF - AUTOPHAGY J2 - AUTOPHAGY VL - AiP PY - 2024 PG - 12 SN - 1554-8627 DO - 10.1080/15548627.2024.2322493 UR - https://m2.mtmt.hu/api/publication/34724664 ID - 34724664 N1 - Institute of Genetics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Doctoral School of Biology, University of Szeged, Szeged, Hungary Institute of Biophysics, HUN-REN Biological Research Centre Szeged, Szeged, Hungary Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary HUN-REN Biophysical Virology Research Group, Budapest, Hungary Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary Export Date: 27 March 2024 Correspondence Address: Juhász, G.; HUN-REN Biological Research Centre Szeged, Temesvari krt. 62, Hungary; email: juhasz.gabor@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Deli, Mária Anna AU - Porkoláb, Gergő AU - Kincses, András AU - Mészáros, Mária AU - Szecskó, Anikó AU - Kocsis, Anna AU - Vigh, Judit Piroska AU - Valkai, Sándor AU - Veszelka, Szilvia AU - Walter, Fruzsina AU - Dér, András TI - Lab-on-a-chip models of the blood-brain barrier: evolution, problems, perspectives JF - LAB ON A CHIP J2 - LAB CHIP VL - 24 PY - 2024 IS - 5 SP - 1030 EP - 1063 PG - 34 SN - 1473-0197 DO - 10.1039/d3lc00996c UR - https://m2.mtmt.hu/api/publication/34673907 ID - 34673907 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [K-143766, K-124922, FK-143233, PD-138930, PD-143268]; Hungarian Research Network [SA-111/2021]; Centenarian Foundation; Talentum Foundation of Gedeon Richter Plc. [UNKP-23-3-SZTE-517, UNKP-23-3-SZTE-535]; National Academy of Scientist Education Program of the National Biomedical Foundation under Hungarian Ministry of Culture and Innovation; New National Excellence Program of the Hungarian Ministry of Culture and Innovation; [H-1103]; [19-21]; [UNKP-23-3-SZTE-497] Funding text: The following funding was received from the National Research, Development and Innovation Office, Hungary: grants K-143766 (to M. A. D.), K-124922 (to A. D.), FK-143233 (to S. V.), PD-138930 (to M. M.), PD-143268 (to A. K.). F. R. W. was supported by the grant SA-111/2021 from the Hungarian Research Network. M. M. was supported by the Centenarian Foundation, A. S. and J. P. V. by the Talentum Foundation of Gedeon Richter Plc. (H-1103 Budapest, Gyoemr & odblac;i str. 19-21. Hungary). G. P. was supported by the National Academy of Scientist Education Program of the National Biomedical Foundation under the sponsorship of the Hungarian Ministry of Culture and Innovation. The New National Excellence Program of the Hungarian Ministry of Culture and Innovation supported G. P. (UNKP-23-3-SZTE-497), A. S. (UNKP-23-3-SZTE-517), and J. P. V. (UNKP-23-3-SZTE-535). LA - English DB - MTMT ER - TY - JOUR AU - Sebőkné Nagy, Krisztina AU - Kóta, Zoltán AU - Kincses, András AU - Fazekas, Ákos Ferenc AU - Dér, András AU - László, Zsuzsanna AU - Páli, Tibor TI - Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 19 EP - 17 PG - 17 SN - 1420-3049 DO - 10.3390/molecules28196750 UR - https://m2.mtmt.hu/api/publication/34153921 ID - 34153921 N1 - Funding Agency and Grant Number: We would like to acknowledge the support provided by the National Research, Development, and Innovation Office of Hungary-PD-143268. [PD-143268]; National Research, Development, and Innovation Office of Hungary Funding text: We would like to acknowledge the support provided by the National Research, Development, and Innovation Office of Hungary-PD-143268. AB - The accumulation of proteins in filter membranes limits the efficiency of filtering technologies for cleaning wastewater. Efforts are ongoing to coat commercial filters with different materials (such as titanium dioxide, TiO2) to reduce the fouling of the membrane. Beyond monitoring the desired effect of the retention of biomolecules, it is necessary to understand what the biophysical changes are in water-soluble proteins caused by their interaction with the new coated filter membranes, an aspect that has received little attention so far. Using spin-label electron paramagnetic resonance (EPR), aided with native fluorescence spectroscopy and dynamic light scattering (DLS), here, we report the changes in the structure and dynamics of bovine serum albumin (BSA) exposed to TiO2 (P25) nanoparticles or passing through commercial polyvinylidene fluoride (PVDF) membranes coated with the same nanoparticles. We have found that the filtering process and prolonged exposure to TiO2 nanoparticles had significant effects on different regions of BSA, and denaturation of the protein was not observed, neither with the TiO2 nanoparticles nor when passing through the TiO2-coated filter membranes. LA - English DB - MTMT ER - TY - JOUR AU - Misra, Ramprasad AU - Das, Ishita AU - Dér, András AU - Steinbach, Gábor AU - Shim, Jin-gon AU - Busse, Wayne AU - Jung, Kwang-Hwan AU - Zimányi, László AU - Sheves, Mordechai TI - Impact of protein–chromophore interaction on the retinal excited state and photocycle of Gloeobacter rhodopsin: role of conserved tryptophan residues JF - CHEMICAL SCIENCE J2 - CHEM SCI VL - 14 PY - 2023 SP - 9951 EP - 9958 PG - 8 SN - 2041-6520 DO - 10.1039/D3SC02961A UR - https://m2.mtmt.hu/api/publication/34129738 ID - 34129738 N1 - Funding Agency and Grant Number: Kimmelman Center for Biomolecular Structure and Assembly, Israel; National Research and Development Office, Hungary [NKFI-1 K-124922, 2018-1.2.1-NKP-2018-00009]; Ministry for National Economy of Hungary [GINOP-2.3.2-15-2016-00001]; Katzir-Makineni Chair in Chemistry Funding text: The work was.nancially supported by the Kimmelman Center for Biomolecular Structure and Assembly, Israel, the National Research and Development Office, Hungary (NKFI-1 K-124922 and 2018-1.2.1-NKP-2018-00009), and the Ministry for National Economy of Hungary (GINOP-2.3.2-15-2016-00001). RM thanks Prof. P. Hegemann for constant encouragements. M. S. holds the Katzir-Makineni Chair in Chemistry. AB - The conserved tryptophan residues in the vicinity of the retinal in microbial rhodopsins affect the retinal excited state charge distribution, increase the isomerization quantum yield, and affect the retinal double bond reisomerization rate during photocycle. LA - English DB - MTMT ER - TY - JOUR AU - Petrovszki, Dániel AU - Valkai, Sándor AU - Kelemen, Lóránd AU - Nagy, László AU - Agarwal, Vivechana AU - Krekic, Szilvia AU - Zimányi, László AU - Dér, András TI - Microsecond All-Optical Modulation by Biofunctionalized Porous Silicon Microcavity JF - NANOMATERIALS J2 - NANOMATERIALS-BASEL VL - 13 PY - 2023 IS - 14 PG - 11 SN - 2079-4991 DO - 10.3390/nano13142070 UR - https://m2.mtmt.hu/api/publication/34064958 ID - 34064958 N1 - Funding Agency and Grant Number: National Research and Development Office, Hungary [NKFI-1 K-124922, ELKH KO-36/2021] Funding text: National Research and Development Office, Hungary (NKFI-1 K-124922) and Eotvos Lorand Research Network (ELKH KO-36/2021). AB - We successfully created a composite photonic structure out of porous silicon (PSi) microcavities doped by the photochromic protein, photoactive yellow protein (PYP). Massive incorporation of the protein molecules into the pores was substantiated by a 30 nm shift of the resonance dip upon functionalization, and light-induced reflectance changes of the device due to the protein photocycle were recorded. Model calculations for the photonic properties of the device were consistent with earlier results on the nonlinear optical properties of the protein, whose degree of incorporation into the PSi structure was also estimated. The successful proof-of-concept results are discussed in light of possible practical applications in the future. LA - English DB - MTMT ER - TY - JOUR AU - Dér, András TI - Keszthelyi Lajos, a karizmatikus vezető és tanítómester JF - FIZIKAI SZEMLE J2 - FIZIKAI SZEMLE VL - 73 PY - 2023 IS - 3 SP - 101 EP - 106 PG - 6 SN - 0015-3257 UR - https://m2.mtmt.hu/api/publication/34025208 ID - 34025208 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Krekic, Szilvia AU - Mero, Mark AU - Kuhl, Michel AU - Balasubramanian, Kannan AU - Dér, András AU - Heiner, Zsuzsanna TI - Photoactive Yellow Protein Adsorption at Hydrated Polyethyleneimine and Poly-l-Glutamic Acid Interfaces JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 10 PG - 16 SN - 1420-3049 DO - 10.3390/molecules28104077 UR - https://m2.mtmt.hu/api/publication/34012278 ID - 34012278 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [NKFI-1 K-124922]; Eotvos Lorand Research Network [ELKH KOE-36/2021]; Deutsche Forschungsgemeinschaft (DFG) [GSC 1013 SALSA, INST 276/754-1]; German Academic Exchange Service (DAAD); Eotvos Hungarian State Scholarship of Tempus Public Foundation - Hungarian Government Funding text: This work received funding from the National Research, Development and Innovation Office, Hungary (NKFI-1 K-124922), the Eotvos Lorand Research Network (ELKH KOE-36/2021), the Deutsche Forschungsgemeinschaft (DFG, no. GSC 1013 SALSA and INST 276/754-1), German Academic Exchange Service (DAAD), and the Eotvos Hungarian State Scholarship of Tempus Public Foundation funded by the Hungarian Government. AB - Chiral and achiral vibrational sum-frequency generation (VSFG) spectroscopy was performed in the 1400–1700 and 2800–3800 cm−1 range to study the interfacial structure of photoactive yellow protein (PYP) adsorbed on polyethyleneimine (PEI) and poly-l-glutamic acid (PGA) surfaces. Nanometer-thick polyelectrolyte layers served as the substrate for PYP adsorption, with 6.5-pair layers providing the most homogeneous surfaces. When the topmost material was PGA, it acquired a random coil structure with a small number of β2-fibrils. Upon adsorption on oppositely charged surfaces, PYP yielded similar achiral spectra. However, the VSFG signal intensity increased for PGA surfaces with a concomitant redshift of the chiral Cα-H and N–H stretching bands, suggesting increased adsorption for PGA compared to PEI. At low wavenumbers, both the backbone and the side chains of PYP induced drastic changes to all measured chiral and achiral VSFG spectra. Decreasing ambient humidity led to the loss of tertiary structure with a re-orientation of α-helixes, evidenced by a strongly blue-shifted chiral amide I band of the β-sheet structure with a shoulder at 1654 cm−1. Our observations indicate that chiral VSFG spectroscopy is not only capable of determining the main type of secondary structure of PYP, i.e., β-scaffold, but is also sensitive to tertiary protein structure. LA - English DB - MTMT ER - TY - JOUR AU - Ughy, Bettina AU - Nagyapáti, Sarolta AU - Lajkó, Dézi Bianka AU - Letoha, Tamas AU - Prohaszka, Adam AU - Deeb, Dima AU - Dér, András AU - Pettkó-Szandtner, Aladár AU - Szilák, László TI - Reconsidering Dogmas about the Growth of Bacterial Populations JF - CELLS J2 - CELLS-BASEL VL - 12 PY - 2023 IS - 10 PG - 15 SN - 2073-4409 DO - 10.3390/cells12101430 UR - https://m2.mtmt.hu/api/publication/33846356 ID - 33846356 N1 - Funding Agency and Grant Number: Hungarian Ministry for National Economy and National Research Development and Innovation Office of Hungary [GINOP-2.3.2-15-2016-00058]; European Union [101000501] Funding text: This research was funded by grants of the Hungarian Ministry for National Economy and National Research Development and Innovation Office of Hungary (GINOP-2.3.2-15-2016-00058) and the European Unions Horizon 2020 Research and Innovation Program under grant agreement No 101000501. AB - The growth of bacterial populations has been described as a dynamic process of continuous reproduction and cell death. However, this is far from the reality. In a well fed, growing bacterial population, the stationary phase inevitably occurs, and it is not due to accumulated toxins or cell death. A population spends the most time in the stationary phase, where the phenotype of the cells alters from the proliferating ones, and only the colony forming unit (CFU) decreases after a while, not the total cell concentration. A bacterial population can be considered as a virtual tissue as a result of a specific differentiation process, in which the exponential-phase cells develop to stationary-phase cells and eventually reach the unculturable form. The richness of the nutrient had no effect on growth rate or on stationary cell density. The generation time seems not to be a constant value, but it depended on the concentration of the starter cultures. Inoculations with serial dilutions of stationary populations reveal a so-called minimal stationary cell concentration (MSCC) point, up to which the cell concentrations remain constant upon dilutions; that seems to be universal among unicellular organisms. LA - English DB - MTMT ER - TY - JOUR AU - Kincses, András AU - Vigh, Judit Piroska AU - Petrovszki, Dániel AU - Valkai, Sándor AU - Kocsis, Anna AU - Walter, Fruzsina AU - Lin, Hung-Yin AU - Jan, Jeng-Shiung AU - Deli, Mária Anna AU - Dér, András TI - The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions JF - BIOSENSORS J2 - BIOSENSORS-BASEL VL - 13 PY - 2023 IS - 3 PG - 17 SN - 2079-6374 DO - 10.3390/bios13030357 UR - https://m2.mtmt.hu/api/publication/33688148 ID - 33688148 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Office, Hungary [NNE-129617, K-124922, PD-143268, SA-111/2021]; National Science Technology Council, Taiwan [NSTC107-2923-M-006-002-MY3] Funding text: The following funding was received from the National Research, Development and Innovation Office, Hungary: grants NNE-129617 (to M.A.D.), K-124922 (to A.D.), PD-143268 (to A.K.). F.R.W. was supported by the grant SA-111/2021 from Lorand Eotvos Research Network, Hungary. J.S.J. was supported by the National Science Technology Council, Taiwan: NSTC107-2923-M-006-002-MY3. AB - The application of lab-on-a-chip technologies in in vitro cell culturing swiftly resulted in improved models of human organs compared to static culture insert-based ones. These chip devices provide controlled cell culture environments to mimic physiological functions and properties. Models of the blood-brain barrier (BBB) especially profited from this advanced technological approach. The BBB represents the tightest endothelial barrier within the vasculature with high electric resistance and low passive permeability, providing a controlled interface between the circulation and the brain. The multi-cell type dynamic BBB-on-chip models are in demand in several fields as alternatives to expensive animal studies or static culture inserts methods. Their combination with integrated biosensors provides real-time and noninvasive monitoring of the integrity of the BBB and of the presence and concentration of agents contributing to the physiological and metabolic functions and pathologies. In this review, we describe built-in sensors to characterize BBB models via quasi-direct current and electrical impedance measurements, as well as the different types of biosensors for the detection of metabolites, drugs, or toxic agents. We also give an outlook on the future of the field, with potential combinations of existing methods and possible improvements of current techniques. LA - English DB - MTMT ER -