TY - JOUR AU - Visnyei, Merve AU - Bakonyi, Péter AU - Rózsenberszki, Tamás AU - Koók, László AU - Komáromy, Péter AU - Bélafiné Bakó, Katalin AU - Nemestóthy, Nándor TI - Mitigated CH4 release of anaerobic waste fermentation is enabled through effluent degassing system equipped with a polydimethylsiloxane membrane contactor JF - CHEMICAL ENGINEERING JOURNAL ADVANCES J2 - CHEM ENG J ADV VL - 18 PY - 2024 SP - 100607 SN - 2666-8211 DO - 10.1016/j.ceja.2024.100607 UR - https://m2.mtmt.hu/api/publication/34794782 ID - 34794782 LA - English DB - MTMT ER - TY - JOUR AU - Szakács, Szabolcs AU - Martínez, Eduardo Ortega AU - Koók, László AU - Santos, Gabriela Medeiros AU - Alarcon, Javiera Toledo AU - Jeison, David AU - Pientka, Zbynek AU - Nemestóthy, Nándor AU - Bélafiné Bakó, Katalin AU - Bakonyi, Péter TI - Biofouling-focused assessment of a novel, cellulose-based ionogel membrane applied in a microbial fuel cell JF - BIORESOURCE TECHNOLOGY REPORTS J2 - BIORESOURCE TECHNO REP VL - 26 PY - 2024 SP - 101817 SN - 2589-014X DO - 10.1016/j.biteb.2024.101817 UR - https://m2.mtmt.hu/api/publication/34753995 ID - 34753995 LA - English DB - MTMT ER - TY - CHAP AU - Szakács, Szabolcs AU - Bakonyi, Péter AU - Bélafiné Bakó, Katalin AU - Nemestóthy, Nándor TI - Cellulóz feldolgozhatósága és hasznosítása ionos folyadék felhasználásával = Processability and utilization of cellulose using ionic liquid T2 - Műszaki Kémiai Napok 2023 Konferencia PB - Pannon Egyetem CY - Veszprém SN - 9789633962565 PY - 2023 SP - 77 UR - https://m2.mtmt.hu/api/publication/34734774 ID - 34734774 AB - Cellulóz kémiailag és fizikailag ellenálló összetett szénhidrát, amely a növények vázanyagát adják, ezáltal a Föld leggyakoribb szerves vegyülete. Gyakorisága okán könnyen és olcsón elérhető, de kémiai ellenállóságának kö- szönhetően a vegyipari hasznosíthatósága limitált. Az elmúlt évtizedekben sokat kutatott ionos folyadékok képe- sek a cellulózt kolloid oldatba vinni viszonylag alacsony hőmérsékleten (~100 °C), ezáltal utat nyitva további felhasználási területek felé. E terület lehet például membrán technológiai, amelyben a korábban említett kolloid oldat vízfürdőben fázis inverziót követően gél állapotba kerül, ezáltal formázható és membránként hasznosítható bioelektrokémiai rendszerben. A kolloid oldatkészítési eljárás akár különféle cellulóz alapú hulladékok feldolgo- zására is alkalmas lehet, illetve a hőkezelési paraméterek változtatásával a cellulóz szénhidrát egységekre is bontható, amely még további lehetőségeket nyit meg miközben textil, élelmiszeripari és fa/papír ipari hulladék hasznosul. Summary Cellulose is chemically and physically the most resilient complex carbohydrate, which is the frame material of various plants, thus this is the Earths most abundant organic compound. Thanks to its abundance this material is cheap and easily accessible, but because of its high chemical resilience its chemical utilization is limited. In the last couple centuries ionic liquids are commonly researched topic, one of the reasons is because it can make a colloid solution with cellulose relatively on low temperature (~100 °C), thus opening certain areas of utilization. One field is on membrane technology in which the aforementioned colloid solution under water suffers phase inversion and leads to a gel state in which the material can be shaped and used as membrane in bio electrochemi- cal systems. This colloid solution process allows certain cellulose based wastes utilization as well and if the curing parameters are changed the cellulose can be degraded into individual carbohydrates which can lead to another utilization field all that while cellulose based wastes have been refurbished. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hülberné Beyer, Éva Anna AU - Nemestóthy, Nándor AU - Bélafiné Bakó, Katalin TI - Case Study of Continuous Itaconic Acid Fermentation by Aspergillus Terreus in a Bench-Scale Bioreactor JF - HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY J2 - HUNG J IND CHEM VL - 51 PY - 2023 IS - 2 SP - 57 EP - 63 PG - 7 SN - 0133-0276 DO - 10.33927/hjic-2023-19 UR - https://m2.mtmt.hu/api/publication/34564462 ID - 34564462 AB - Itaconic acid (IA) is a highly important bioproduct. The traditional biotechnological production of organic acids generates large quantities of biomass and water streams as waste. Bipolar membrane electrodialysis is a suitable technique for directly recovering IA from clarified fermentation broth whereby itaconic acid is separated without chemical loading. As a result, the remaining diluate stream can be reintroduced into the fermentation process. This membrane operation can be integrated into a continuous fermentation process in order to carry out the process using a high glucose concentration in the effluent, opening up new possibilities for the implementation of continuous itaconic acid fermentation. In this study, a possible highly successful implementation of this technique is presented. The applied gentle stirring technique as well as the low dilution rate of 0.007 s-1 is necessary to be complemented by highly efficient oxygenation, thereby promoting itaconic acid generation. With an inlet supplying pure oxygen gas, the acid accumulated and an average of 30.1 g·L-1 titer of IA was achieved in the effluent. The volumetric oxygen uptake rate was monitored during the fermentation which fluctuated from 40-88% so further investigation of this may be worthwhile in the future. LA - English DB - MTMT ER - TY - JOUR AU - Furgani, G.M. AU - Makrai, L. AU - Vozik, Dávid AU - Kiss, J. AU - Olasz, F. AU - Klein, M.G. AU - Ghaffar, M.B. AU - Szabados, L. AU - Noureldeen, A. AU - Bélafiné Bakó, Katalin AU - Fodor, L. AU - Forst, S.A. AU - Fodor, A. TI - Agrobacterium Is a Proposed Suitable System for Bioassays and Resistance Studies on Antimicrobial Peptides with Plant Protection Potential JF - PREPRINTS J2 - PREPRINTS PY - 2023 SN - 2310-287X DO - 10.20944/preprints202311.0120.v1 UR - https://m2.mtmt.hu/api/publication/34523826 ID - 34523826 AB - (1) Background: The biosynthetic antimicrobial compounds released by Xenorhabdus budapestensis (EMA) are mostly non-ribosomal templated oligopeptides, (NR-AMPs). We are interested in their agricultural application potential. (2) Methods: To concentrate the antimicrobial potential the native cell-free culture media (CFCM) of EMA peptide-fraction were isolated by amberlite adsorption and following washing and elution, subfractions were separated an bioassayed on different test organisms. Antibacterial potentials were determined (by the agar-diffusion technique) of the following samples: EMA-PF2 peptide fraction and its subfractions (with reproducible HPLC, and MALDI profiles). The test organisms were bio-assayed on Gram-positive, multi-resistant Gram-negative laboratory bacterium strains, and Candida targets for sensitive/resistant (S/R) phenotypes. The same was screened for in the wild-type Agrobacterium strain A281, disarmed derivatives, and plasmid-cured strains of different genotypes and compared. Liquid culture bioassays and agar-diffusion data were compared. (3) Results: (a) The EMA_CFCM is an abundant source of AMPs usable as plant-protecting biopreparations (like compost supplements). HPLC and RP-HPLC purified fractions were extremely potent on the tested organisms. (b) The wild-type and plasmid-cured Agrobacterium strains proved resistant; all the studied disarmed strains proved sensitive to EMA PF2. (4) Discussion: =(a) The anti-Gram-positive and anti-Gram-negative activities could not be separated in such a way, that all the antimicrobialactive subfractions were effective against each target. (b) Neither the chromosome (C58 or Ach5) nor the opine type seems to be a determining factor of EMA-PF2 resistance/sensitivity, but the TIplasmid genotype seems to be important. (5) Conclusions (a) No cross-resistances were observed with clinically used antibiotics, which is an argument for the application potential of EMA-derived AMPs against plant pathogen bacteria. (b) The surprising S phenotype of the disarmed Agrobacterium strains may provide an exceptional option for genetic (complementation) analysis but these data should be confirmed by similar results of similar experiments carried out at all the available helper-plasmid harboring strains in different labs. LA - English DB - MTMT ER - TY - JOUR AU - Hülberné Beyer, Éva Anna AU - Bélafiné Bakó, Katalin AU - Rózsenberszki, Tamás AU - Komáromy, Péter AU - Nemestóthy, Nándor TI - Evaluating the potential of semi-continuous itaconic acid fermentation by Aspergillus terreus: operational profile and experiences JF - WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY J2 - WORLD J MICROB BIOT VL - 39 PY - 2023 IS - 12 PG - 11 SN - 0959-3993 DO - 10.1007/s11274-023-03797-9 UR - https://m2.mtmt.hu/api/publication/34204453 ID - 34204453 AB - Itaconic acid is an important bio-based chemical. The present study aims to evaluate the applicability of semi-continuous fermentation technique for itaconic acid production by Aspergillus terreus. The fermentation is planned to be connected with bipolar membrane electrodialysis unit for acid recovery. This process allows the reuse of residual glucose from the effluent. Our particular attention was focused on the effect of glucose concentration. Two different glucose supplementation strategies were tested: constant glucose concentration in the refilling medium and adjusted glucose concentration in order to maintain a continuously high – 120 g/L – glucose concentration in the fermentor. The itaconic acid titre, yield and productivity for the 24 h time periods between draining/refilling interventions were investigated. The constantly high glucose concentration in the fermentor resulted in doubled biomass formation. The average itaconic acid titre was 32.9 ± 2.7 g/L. The producing strain formed numerous spores during semi-continuous fermentation that germinated continuously. Yield and volumetric productivity showed a periodic pattern during the procedure. LA - English DB - MTMT ER - TY - JOUR AU - Koók, László AU - Nagy, Kristóf Bence AU - Nyirő-Kósa, Ilona AU - Kovács, Szilveszter AU - Žitka, Jan AU - Otmar, Miroslav AU - Bakonyi, Péter AU - Nemestóthy, Nándor AU - Bélafiné Bakó, Katalin TI - Simultaneous Urea and Phosphate Recovery from Synthetic Urine by Electrochemical Stabilization JF - MEMBRANES (BASEL) J2 - MEMBRANES-BASEL VL - 13 PY - 2023 IS - 8 SP - 699 SN - 2077-0375 DO - 10.3390/membranes13080699 UR - https://m2.mtmt.hu/api/publication/34086157 ID - 34086157 AB - Urine is a widely available renewable source of nitrogen and phosphorous. The nitrogen in urine is present in the form of urea, which is rapidly hydrolyzed to ammonia and carbonic acid by the urease enzymes occurring in nature. In order to efficiently recover urea, the inhibition of urease must be done, usually by increasing the pH value above 11. This method, however, usually is based on external chemical dosing, limiting the sustainability of the process. In this work, the simultaneous recovery of urea and phosphorous from synthetic urine was aimed at by means of electrochemical pH modulation. Electrochemical cells were constructed and used for urea stabilization from synthetic urine by the in situ formation of OH- ions at the cathode. In addition, phosphorous precipitation with divalent cations (Ca2+, Mg2+) in the course of pH elevation was studied. Electrochemical cells equipped with commercial (Fumasep FKE) and developmental (PSEBS SU) cation exchange membranes (CEM) were used in this study to carry out urea stabilization and simultaneous P-recovery at an applied current density of 60 A m−2. The urea was successfully stabilized for a long time (more than 1 month at room temperature and nearly two months at 4 °C) at a pH of 11.5. In addition, >82% P-recovery could be achieved in the form of precipitate, which was identified as amorphous calcium magnesium phosphate (CMP) by using transmission electron microscopy (TEM). LA - English DB - MTMT ER - TY - JOUR AU - Rózsenberszki, Tamás AU - Komáromy, Péter AU - Hülberné Beyer, Éva Anna AU - Pesti, Andrea AU - Koók, László AU - Bakonyi, Péter AU - Bélafiné Bakó, Katalin AU - Nemestóthy, Nándor TI - Bipolar membrane electrodialysis integration into the biotechnological production of itaconic acid: a proof-of-concept study JF - CHEMICAL ENGINEERING RESEARCH AND DESIGN J2 - CHEM ENG RES DES VL - 2023 PY - 2023 SN - 0263-8762 DO - 10.1016/j.cherd.2022.12.023 UR - https://m2.mtmt.hu/api/publication/33531397 ID - 33531397 LA - English DB - MTMT ER - TY - JOUR AU - Bakonyi, Péter AU - Koók, László AU - Rózsenberszki, Tamás AU - Kalauz-Simon, Veronika AU - Bélafiné Bakó, Katalin AU - Nemestóthy, Nándor TI - CO2-refinery through microbial electrosynthesis (MES): A concise review on design, operation, biocatalysts and perspectives JF - JOURNAL OF CO2 UTILIZATION J2 - J CO2 UTIL VL - 67 PY - 2023 SN - 2212-9820 DO - 10.1016/j.jcou.2022.102348 UR - https://m2.mtmt.hu/api/publication/33295669 ID - 33295669 LA - English DB - MTMT ER - TY - CHAP AU - Visnyei, Merve AU - Bakonyi, Péter AU - Nemestóthy, Nándor AU - Bélafiné Bakó, Katalin ED - Balogh, András ED - Klein, Mónika TI - Separation of dissolved gases from aqueous anaerobic effluents using gas-liquid membrane contactors T2 - 50. Műszaki Kémiai Napok Jubileumi Konferencia PB - Pannon Egyetem Mérnöki Kar CY - Veszprém SN - 9789633962343 PY - 2022 SP - 77 UR - https://m2.mtmt.hu/api/publication/33823942 ID - 33823942 LA - English DB - MTMT ER -