TY - JOUR AU - Kocsis, László AU - Bruckdorfer, T AU - Orosz, György TI - The concept of internal solubilization in peptide synthesis: ethylene glycol-based protecting groups JF - TETRAHEDRON LETTERS J2 - TETRAHEDRON LETT VL - 49 PY - 2008 SP - 7015 EP - 7017 PG - 3 SN - 0040-4039 UR - https://m2.mtmt.hu/api/publication/117352 ID - 117352 LA - English DB - MTMT ER - TY - JOUR AU - Rónai, András AU - Szemenyei, Erzsébet AU - Kató, Erzsébet AU - Kocsis, László AU - Orosz, György AU - Al-Khrasani, Mahmoud AU - Tóth, Géza TI - Endomorphin synthesis in rat brain from intracerebroventricularly injected [H-3]-Tyr-Pro: A possible biosynthetic route for endomorphins JF - REGULATORY PEPTIDES J2 - REGUL PEPTIDES VL - 134 PY - 2006 IS - 1 SP - 54 EP - 60 PG - 7 SN - 0167-0115 DO - 10.1016/j.regpep.2005.12.004 UR - https://m2.mtmt.hu/api/publication/1626916 ID - 1626916 N1 - Cited By :17 Export Date: 29 May 2022 CODEN: REPPD AB - in spite of concentrated efforts, the biosynthetic route of mu-opioid receptor agonist brain tetrapeptide endomorphins (Tyr-Pro-Trp-Phe-NH2 and Tyr-Pro-Phe-Phe-NH2), discovered in 1997, is still obscure. We report presently that 30 min after intracerebroventricular injection of 20 or 200 mu Ci [H-3]Tyr-Pro (49.9 Ci mmol(-1)) the incorporated radioactivity was found in endomorphin-related tetra- and tripeptides in rat brain extracts. As detected by the combination of HPLC with radiodetection, a peak corresponding to endomorphin-2-OH could be identified in two of four extracts of "20 mu Ci" series. Radioactive peaks in position of Tyr, Tyr-Pro, Tyr-Pro-Phe or Tyr-Pro-Trp appeared regularly in both series and also in the "tetrapeptide cluster" constituted by endomorphins and their free carboxylic forms. In one of the four extracts in the "200 mu Ci" series a robust active peak in the position of endomorphin 2 could be detected. Intracerebroventricularly injected 100 mmol, but not 10 or 1000 nmol cold Tyr-Pro (devoid of opioid activity in vitro), caused a naloxone-reversible prolongation of tail-flick latency in rats, peaking between 15 and 30 min. We suggest that Tyr-Pro may serve as a biosynthetic precursor to endomorphin synthesis. (C) 2006 Elsevier B.V. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Rónai, András AU - Al-Khrasani, Mahmoud AU - Benyhe, Sándor AU - Lengyel, Imre AU - Kocsis, L AU - Orosz, György AU - Tóth, Géza AU - Kató, Erzsébet AU - Tóthfalusi, László TI - Partial and full agonism in endomorphin derivatives: Comparison by null and operational model JF - PEPTIDES J2 - PEPTIDES VL - 27 PY - 2006 IS - 6 SP - 1507 EP - 1513 PG - 7 SN - 0196-9781 DO - 10.1016/j.peptides.2005.12.003 UR - https://m2.mtmt.hu/api/publication/1599754 ID - 1599754 N1 - Cited By :17 Export Date: 29 May 2022 CODEN: PEPTD AB - The partial mu-opioid receptor pool inactivation strategy in isolated mouse vas deferens was used to determine partial agonism of endomorphins and their analogs (endomorphin-1-ol, 2',6'-dimethyltyrosine (Dmt)-endomorphin-1, endomorphin-2-ol and (D-Met 2)-endomorphin-2) using morphine, normorphine, morphiceptin, (D-Ala(2), MePhe(4) Glys-ol)-enkephal\in (DAMGO) and its amide (DAMGA) as reference opioid agonists. Agonist affinities (KA) and efficacies were assessed both by the "null" and the "operational" method. The KA values determined by the two methods correlated significantly with each other and also with the displacing potencies against H-3-naloxone in the receptor binding assay in the presence of Na+. DAMGO and DAMGA were full agonist prototypes, morphine, endomorphin-1, endomorphin-1-ol, Dmt-endomorphin-1, endomorphin-2-ol and (D-Met(2))-endomorphin-2 were found by both methods to be partial agonists whereas the parameters for normorphine, morphiceptin and endomorphin-2 were intermediate. (c) 2005 Elsevier Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Gündüz, Özge AU - Sipos, F AU - Spagnolo, B AU - Kocsis, László AU - Magyar, Anna (Biczókné) AU - Orosz, György AU - Borsodi, Anna AU - Calo, G AU - Benyhe, Sándor TI - In vitro binding and functional studies of Ac-RYYRIK-ol and its derivatives, Novel partial agonists of the nociceptin/orphanin F/Q receptor JF - NEUROSIGNALS J2 - NEUROSIGNALS VL - 15 PY - 2006 IS - 2 SP - 91 EP - 101 PG - 11 SN - 1424-862X DO - 10.1159/000094743 UR - https://m2.mtmt.hu/api/publication/117616 ID - 117616 N1 - Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Budapest, Hungary Reanal Finechemical Co., Budapest, Hungary Department of Experimental and Clinical Medicine, Section of Pharmacology and Neuroscience Center, University of Ferrara, Ferrara, Italy Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, HU-6701 Szeged, Hungary Cited By :9 Export Date: 9 October 2019 CODEN: NEURI Correspondence Address: Gündüz, Ö.; Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, HU-6701 Szeged, Hungary; email: ozge@nucleus.szbk.u-szeged.hu LA - English DB - MTMT ER - TY - CHAP AU - KOCSIS, L AU - Magyar, Anna (Biczókné) AU - Orosz, György ED - Epton, R TI - PEG-PS resins in the synthesis of different peptide sequences. Innovation and Perspectives in Solid Phase Synthesis and Combinatorial Libraries TS - Innovation and Perspectives in Solid Phase Synthesis and Combinatorial Libraries T2 - Innovation and Perspectives in Solid Phase Synthesis & Combinatorial Libraries : peptides, proteins and nucleic acids : small molecule organic chemical diversity: 2004 PB - Mayflower Worldwide Ltd CY - Kingswinford SN - 0951573551 PY - 2004 SP - 263 EP - 264 PG - 2 UR - https://m2.mtmt.hu/api/publication/3115803 ID - 3115803 LA - English DB - MTMT ER - TY - JOUR AU - Kocsis, László AU - Magyar, Anna (Biczókné) AU - Orosz, György TI - Ethylene glycol based protecting groups JF - JOURNAL OF PEPTIDE SCIENCE J2 - J PEPT SCI VL - 10 PY - 2004 IS - S2 SP - 128 SN - 1075-2617 UR - https://m2.mtmt.hu/api/publication/117570 ID - 117570 LA - English DB - MTMT ER - TY - JOUR AU - Kocsis, László AU - Orosz, György AU - Magyar, Anna (Biczókné) AU - Al-Khrasani, Mahmoud AU - Kató, Erzsébet AU - Rónai, András AU - BES, B AU - MEUNIER, JC AU - Gündüz, Özge AU - Tóth, Géza AU - Borsodi, Anna AU - Benyhe, Sándor TI - Nociceptin antagonism: probing the receptor by N-acetyl oligopeptides JF - REGULATORY PEPTIDES J2 - REGUL PEPTIDES VL - 122 PY - 2004 SP - 199 EP - 207 PG - 9 SN - 0167-0115 DO - 10.1016/j.regpep.2004.06.019 UR - https://m2.mtmt.hu/api/publication/117569 ID - 117569 LA - English DB - MTMT ER - TY - JOUR AU - Benyhe, S AU - Gunduz, O AU - Farkas, J AU - Kocsis, László AU - Sipos, F AU - Ligeti, Melinda AU - Magyar, Anna (Biczókné) AU - Orosz, György AU - Toth, G AU - Borsodi, A TI - Characterization of nociceptin binding sites by novel peptide analogs and radioprobes JF - JOURNAL OF NEUROCHEMISTRY J2 - J NEUROCHEM VL - 85 PY - 2003 IS - 2 SP - 32 EP - 32 PG - 1 SN - 0022-3042 DO - 10.1046/j.1471-4159.85.s2.20_6.x UR - https://m2.mtmt.hu/api/publication/2529091 ID - 2529091 N1 - CT 14th Meeting of the European-Society-for-Neurochemistry CY JUN 01-04, 2003 CL WARSAW, POLAND SU 2 LA - English DB - MTMT ER - TY - CONF AU - Lengyel, A AU - Dudar, E AU - Orosz, György ED - Epton, R TI - Infrared studies on polystyrene matrix T2 - Innovation and Perspectives in Solid Phase Synthesis and Combinatorial Libraries 2001. Proceedings of the 6th International Symposium PB - Mayflower Worldwide Ltd C1 - Kingswinford PY - 2003 SP - 221 EP - 224 PG - 4 UR - https://m2.mtmt.hu/api/publication/121416 ID - 121416 LA - English DB - MTMT ER - TY - JOUR AU - Lengyel, Imre AU - Orosz, György AU - Biyashev, Daureen AU - KOCSIS, L AU - Al-Khrasani, Mahmoud AU - Rónai, András AU - Tömböly, Csaba AU - Fürst, Zsuzsanna AU - Tóth, Géza AU - Borsodi, Anna TI - Side chain modifications change the binding and agonist properties of endomorphin 2 JF - BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS J2 - BIOCHEM BIOPH RES CO VL - 290 PY - 2002 SP - 153 EP - 161 PG - 9 SN - 0006-291X DO - 10.1006/bbrc.2001.6136 UR - https://m2.mtmt.hu/api/publication/1912438 ID - 1912438 AB - Side chain modifications were introduced to endomorphin 2 (E2) to improve its binding properties and biological activity. A number of C-terminal modifications decreased the binding affinity to the mu-opioid receptor and the intrinsic activity in rat brain membranes. The exception was E2-ol, which showed increased binding affinity to MOR and higher potency in stimulating [S-35]GTPgammaS binding. N-methylation of Phe(3) (MePhe(3)) attenuated the binding affinity and produced a rightward shift of [S-35]GTPgammaS binding curves. All derivatives had lower intrinsic activity than E2. Some of the modified peptides partially inhibited, while YPF-benzyl-allyl- amide fully inhibited, the E2 or [D- Ala(2),MePhe(4),Gly(5)ol]enkephalin stimulated [S-35]GTPgammaS binding. Marked differences were found between the results obtained using tritiated E2, tritiated naloxone, and [S- 35]GTPgammaS binding, indicating the possible involvement of multiple binding sites. The data presented demonstrate that the C-terminal amide group has an essential role in the regulation of the binding and the agonist/antagonist properties of E2. (C) 2002 Elsevier Science. LA - English DB - MTMT ER -