TY - CONF AU - Ormai, Edit AU - Kocsis, Béla AU - Böszörményi, Andrea AU - Horváth, Györgyi AU - Balázs, Viktória Lilla ED - Kovács, Bettina ED - Glázer-Kniesz, Adrienn ED - Tislér, Ádám TI - Nozokomiális patogének és a szegfűszeg illóolaj kapcsolatának bemutatása evidenciaalapú megközelítésben T2 - XII. Interdiszciplináris Doktorandusz Konferencia 2024. Április 5-6. Absztraktkötet=XII. Interdisciplinary Doctoral Conference 5-6th of April, 2024 Book of Abstracts PB - Pécsi Tudományegyetem Doktorandusz Önkormányzat C1 - Pécs SN - 9789636262457 PY - 2024 SP - 83 EP - 83 PG - 1 UR - https://m2.mtmt.hu/api/publication/34814410 ID - 34814410 LA - Hungarian DB - MTMT ER - TY - CONF AU - Nagy, Anett AU - Ormai, Edit AU - Horváth, Györgyi AU - Kocsis, Béla AU - Balázs, Viktória Lilla ED - Szabó, Szilvia TI - Négy gyógyszerkönyvi illóolaj antibakteriális hatásának vizsgálata a Pseudomonas aeruginosa baktériummal szemben T2 - Abstract Book of the 7th International Cholnoky Symposium PY - 2024 SP - 23 EP - 23 PG - 1 UR - https://m2.mtmt.hu/api/publication/34795108 ID - 34795108 LA - Hungarian DB - MTMT ER - TY - CONF AU - Koloh, Regina AU - Farkas, Ágnes AU - Kocsis, Béla AU - Kocsis, Marianna AU - Balázs, Viktória Lilla ED - Szabó, Szilvia TI - A gesztenyeméz biofilmgátló hatása az idő függvényében T2 - Abstract Book of the 7th International Cholnoky Symposium PY - 2024 SP - 21 EP - 21 PG - 1 UR - https://m2.mtmt.hu/api/publication/34795054 ID - 34795054 LA - Hungarian DB - MTMT ER - TY - CONF AU - Ormai, Edit AU - Kocsis, Béla AU - Böszörményi, Andrea AU - Horváth, Györgyi AU - Balázs, Viktória Lilla ED - Szabó, Szilvia TI - A borsmenta illóolaj hatása kórházi környezetben előforduló patogéneken T2 - Abstract Book of the 7th International Cholnoky Symposium PY - 2024 SP - 19 EP - 19 PG - 1 UR - https://m2.mtmt.hu/api/publication/34795038 ID - 34795038 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Balázs, Viktória Lilla AU - Böszörményi, Andrea AU - Kocsis, Béla AU - Horváth, Györgyi TI - Gram-negative rough mutants used as test bacteria can increase sensitivity of direct bioautography JF - JPC - JOURNAL OF PLANAR CHROMATOGRAPHY - MODERN TLC J2 - J PLANAR CHROMATOGR MOD TLC PY - 2024 SN - 0933-4173 DO - 10.1007/s00764-024-00293-0 UR - https://m2.mtmt.hu/api/publication/34753258 ID - 34753258 N1 - * Megosztott szerzőség AB - Currently, the antimicrobial activity of essential oils (EOs) is an outstanding research field due to antibiotic resistance of microorganisms. Thin-layer chromatography‒direct bioautography (TLC‒DB) is an effective, fast method to find components with antimicrobial activity in a mixture of plant compounds, e.g., in EOs. The volatility and hydrophobic characters of EOs require special experimental conditions, and disc diffusion assay is not appropriate to explore the antimicrobial activity of them. The aim of this study was to use “R” mutants, which are more sensitive to synthetic antimicrobial drugs, in DB to increase the sensitivity of this method. Our hypothesis was that these mutants show sensitivity to some EOs (thyme, clove, and peppermint) as well. The chemical composition of our tested EOs was measured with gas chromatography‒mass spectrometry (GC‒MS). The main compounds (39.8% thymol, 78.8% eugenol, and 50.4% menthol) of EOs showed notable antibacterial activity in TLC‒DB. Based on our results, we suggest to use Salmonella minnesota Re595 rough strain as test bacterium in bioautography, because it showed the highest sensitivity to the tested antibiotics (gentamicin and cephalexin) and EOs. Furthermore, this rough mutant could make TLC‒DB more faster, because only 4 h incubation time was enough to detect the inhibition zones of the active compounds used in this study. LA - English DB - MTMT ER - TY - JOUR AU - Marosvölgyi, Tamás AU - Mintál, Kitti AU - Borbásné Farkas, Kornélia AU - Sipos, Zoltán AU - Makszin, Lilla AU - Szabó, Éva AU - Tóth, Attila AU - Kocsis, Béla AU - Kovács, Krisztina AU - Hormay, Edina AU - Lénárd, László AU - Karádi, Zoltán György AU - Bufa, Anita TI - Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-024-57046-6 UR - https://m2.mtmt.hu/api/publication/34745300 ID - 34745300 N1 - * Megosztott szerzőség AB - Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host’s physiological processes. LA - English DB - MTMT ER - TY - JOUR AU - Koloh, Regina AU - Balázs, Viktória Lilla AU - Radványi, Lilla AU - Kocsis, Béla AU - Kerekes, Erika Beáta AU - Kocsis, Marianna AU - Farkas, Ágnes TI - Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 13 PY - 2024 IS - 3 PG - 11 SN - 2079-6382 DO - 10.3390/antibiotics13030255 UR - https://m2.mtmt.hu/api/publication/34738553 ID - 34738553 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office NKFIH K [132044]; New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development, and Innovation Fund [UNKP-23-3-I] Funding text: This research was funded by the grant from the National Research, Development, and Innovation Office NKFIH K 132044, and by the UNKP-23-3-I New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development, and Innovation Fund. AB - The irresponsible overuse of antibiotics has increased the occurrence of resistant bacterial strains, which represents one of the biggest patient safety risks today. Due to antibiotic resistance and biofilm formation in bacteria, it is becoming increasingly difficult to suppress the bacterial strains responsible for various chronic infections. Honey was proven to inhibit bacterial growth and biofilm development, offering an alternative solution in the treatment of resistant infections and chronic wounds. Our studies included chestnut honey, valued for its high antibacterial activity, and the bacteria Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and S. epidermidis, known to form multi-species biofilm communities. Minimum inhibitory concentrations (MIC) of chestnut honey were determined for each bacterial strain. Afterwards, the mixed bacterial biofilms were treated with chestnut honey at different stages of maturity (incubation times: 2, 4, 6, 12, 24 h). The extent of biofilm inhibition was measured with a crystal violet assay and demonstrated by scanning electron microscopy (SEM). As the incubation time increased and the biofilm became more mature, inhibition rates decreased gradually. The most sensitive biofilm was the combination MRSA-S. epidermidis, with a 93.5% inhibition rate after 2 h of incubation. Our results revealed that chestnut honey is suitable for suppressing the initial and moderately mature stages of mixed biofilms. LA - English DB - MTMT ER - TY - JOUR AU - Bácsi, Attila AU - Ágics, Beatrix AU - Pázmándi, Kitti Linda AU - Kocsis, Béla AU - Sándor, Viktor AU - Bertók, Lóránd AU - Bruckner, Geza AU - Sipka, Sándor TI - Radiation-Detoxified Form of Endotoxin Effectively Activates Th1 Responses and Attenuates Ragweed-Induced Th2-Type Airway Inflammation in Mice JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 PG - 22 SN - 1661-6596 DO - 10.3390/ijms25031581 UR - https://m2.mtmt.hu/api/publication/34574199 ID - 34574199 N1 - * Megosztott szerzőség AB - Urbanization with reduced microbial exposure is associated with an increased burden of asthma and atopic symptoms. Conversely, environmental exposure to endotoxins in childhood can protect against the development of allergies. Our study aimed to investigate whether the renaturation of the indoor environment with aerosolized radiation-detoxified lipopolysaccharide (RD-LPS) has a preventative effect against the development of ragweed-induced Th2-type airway inflammation. To explore this, cages of six-week-old BALB/c mice were treated daily with aerosolized native LPS (N-LPS) or RD-LPS. After a 10-week treatment period, mice were sensitized and challenged with ragweed pollen extract, and inflammatory cell infiltration into the airways was observed. As dendritic cells (DCs) play a crucial role in the polarization of T-cell responses, in our in vitro experiments, the effects of N-LPS and RD-LPS were compared on human monocyte-derived DCs (moDCs). Mice in RD-LPS-rich milieu developed significantly less allergic airway inflammation than mice in N-LPS-rich or common environments. The results of our in vitro experiments demonstrate that RD-LPS-exposed moDCs have a higher Th1-polarizing capacity than moDCs exposed to N-LPS. Consequently, we suppose that the aerosolized, non-toxic RD-LPS applied in early life for the renaturation of urban indoors may be suitable for the prevention of Th2-mediated allergies in childhood. LA - English DB - MTMT ER - TY - CONF AU - Mérész, Balázs AU - Inez, Bosnyak AU - Molitor, Dorottya AU - Kocsis, Béla AU - Borbásné Farkas, Kornélia AU - Reglődi, Dóra AU - Atlasz, Tamás AU - Váczy, Alexandra TI - Potential new treatment target in bacterial keratitis T2 - 7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet PY - 2024 SP - 69 EP - 69 PG - 1 UR - https://m2.mtmt.hu/api/publication/34566422 ID - 34566422 LA - English DB - MTMT ER - TY - CONF AU - Bosnyák, Inez AU - Váczy, Alexandra AU - Mérész, Balázs AU - Molitor, Dorottya AU - Kovács, Krisztina AU - Kocsis, Béla AU - Reglődi, Dóra AU - Atlasz, Tamás TI - Egy lehetséges új terápiás célpont retinagyulladásban T2 - A Magyar Anatómus Társaság 2023. évi konferenciája PY - 2023 SP - 78 EP - 79 PG - 2 UR - https://m2.mtmt.hu/api/publication/34566631 ID - 34566631 LA - Hungarian DB - MTMT ER -