TY - JOUR AU - Skopkó, Boglárka Emese AU - Homoki, Judit Rita AU - Fazekas, Mónika Éva AU - Paholcsek, Melinda AU - Fauszt, Péter AU - Dávid, Péter AU - Stündl, László AU - Molnár, Piroska Bíróné AU - Forgács, Ildikó Noémi AU - Váradi, Judit AU - Bágyi, Kinga, Ágnes AU - Gálné Remenyik, Judit TI - Changes in the Composition of Unstimulated and Stimulated Saliva Due to Chewing Sour Cherry Gum and a Toothbrush Change JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 3 SN - 2073-4409 DO - 10.3390/cells13030251 UR - https://m2.mtmt.hu/api/publication/34546012 ID - 34546012 AB - Background: Our previous studies demonstrated that sour cherry anthocyanins (AC) reduce the salivary count of Streptococcus mutans and inhibit salivary amylase activity within 30 minutes after chewing AC gum. AC gum and changing toothbrushes after scaling reduced the Gram-negative species in the unstimulated salivary microbiota. The present study examined the effect of AC gums on salivary factors, including changes in microbiome. Methods: The study was conducted over three weeks with two groups; young adults (18–30) and adults (30–45). Ten participants changed their toothbrushes, while the other 10 participants did not change after the control period. After scaling, all participants received three doses of AC gum daily. The salivary mRNA and protein levels of cytokines, mucins, melatonin, and the microbiota of unstimulated and stimulated saliva were determined by polymerase chain reaction, enzyme-linked immunosorbent assay, and 16S rRNA gene sequencing. Results: Significantly higher levels of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β), mucin5B (MUC5B), mucin7 (MUC7), and melatonin were detected in stimulated saliva. Correlation analysis of these factors with the microbiota showed positive correlations with the genera Lachnospiraceae, Eikenella, Saccharibacteria_(TM7), Streptococcus, Prevotella, and Haemophilus. Conclusions: AC chewing gum has a beneficial effect on the composition of the oral microbiome, and toothbrush replacement leads to changes in the levels of salivary pro-inflammatory cytokines. LA - English DB - MTMT ER - TY - JOUR AU - Hermenean, Anca AU - Dossi, Eleftheria AU - Hamilton, Alex AU - Trotta, Maria Consiglia AU - Russo, Marina AU - Lepre, Caterina Claudia AU - Sajtos, Csilla AU - Rusznyák, Ágnes AU - Váradi, Judit AU - Bácskay, Ildikó AU - Budai, István AU - D’Amico, Michele AU - Fenyvesi, Ferenc TI - Chrysin Directing an Enhanced Solubility through the Formation of a Supramolecular Cyclodextrin–Calixarene Drug Delivery System: A Potential Strategy in Antifibrotic Diabetes Therapeutics JF - PHARMACEUTICALS J2 - PHARMACEUTICALS-BASE VL - 17 PY - 2024 IS - 1 SP - 1 EP - 20 PG - 20 SN - 1424-8247 DO - 10.3390/ph17010107 UR - https://m2.mtmt.hu/api/publication/34500966 ID - 34500966 AB - Calixarene 0118 (OTX008) and chrysin (CHR) are promising molecules for the treatment of fibrosis and diabetes complications but require an effective delivery system to overcome their low solubility and bioavailability. Sulfobutylated β-cyclodextrin (SBECD) was evaluated for its ability to increase the solubility of CHR by forming a ternary complex with OTX008. The resulting increase in solubility and the mechanisms of complex formation were identified through phase-solubility studies, while dynamic light-scattering assessed the molecular associations within the CHR-OTX008-SBECD system. Nuclear magnetic resonance, differential scanning calorimetry, and computational studies elucidated the interactions at the molecular level, and cellular assays confirmed the system’s biocompatibility. Combining SBECD with OTX008 enhances CHR solubility more than using SBECD alone by forming water-soluble molecular associates in a ternary complex. This aids in the solubilization and delivery of CHR and OTX008. Structural investigations revealed non-covalent interactions essential to complex formation, which showed no cytotoxicity in hyperglycemic in vitro conditions. A new ternary complex has been formulated to deliver promising antifibrotic agents for diabetic complications, featuring OTX008 as a key structural and pharmacological component. LA - English DB - MTMT ER - TY - JOUR AU - Rusznyák, Ágnes AU - Szászné Réti-Nagy, Katalin AU - Váradi, Judit AU - Bácskay, Ildikó AU - Vecsernyés, Miklós AU - Fenyvesi, Ferenc TI - Hidroxi-propil-béta-ciklodextrin celluláris internalizációjának és lizoszómákra gyakorolt hatásának vizsgálata JF - EGÉSZSÉGÜGYI INNOVÁCIÓS SZEMLE J2 - EÜ INNOV SZLE VL - 2 PY - 2023 IS - 2 SP - 57 EP - 64 PG - 8 SN - 2939-6026 DO - 10.56626/egis.v2i2.12924 UR - https://m2.mtmt.hu/api/publication/34688027 ID - 34688027 AB - A ciklodextrinek széles körben alkalmazott segédanyagok a lipofil gyógyszerek vízoldhatóságának és biológiai hasznosíthatóságának növelésére. Kutatócsoportunk korábban kimutatta, hogy a ciklodextrinek endocitózissal képesek bejutni a Caco-2 intesztinális epitél sejtekbe, azonban a különböző sejtek esetén történő celluláris internalizációt és az intracelluláris hatásait eddig még nem vizsgálták. Mindemellett a 2-hidroxi-propil-béta-ciklodextrin (HPBCD) koleszterin komplexáló tulajdonságának köszönhetően a C típusú Niemann Pick szindróma kezelésére alkalmazott molekula. Munkánk célja a HPBCD celluláris internalizációjának és a sejtekben található lizoszómákra gyakorolt hatásának vizsgálata és összehasonlítása Caco-2 intesztinális epitél, HeLa méhnyak epitél és hCMEC/D3 agyi endotél sejteken. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Klusóczki, Ágnes AU - Oláh, Boglárka AU - Hosszú, Dominik AU - Fenyvesi, Ferenc AU - Gálné Remenyik, Judit AU - Homoki, Judit Rita AU - Gyöngyösi, Alexandra AU - Bácskay, Ildikó AU - Váradi, Judit TI - Effectiveness of Anthocyanin-Rich Sour Cherry Extract on Gliadin-Induced Caco-2 Barrier Damage JF - NUTRIENTS J2 - NUTRIENTS VL - 15 PY - 2023 IS - 18 SN - 2072-6643 DO - 10.3390/nu15184022 UR - https://m2.mtmt.hu/api/publication/34160368 ID - 34160368 AB - Several types of gluten-related disorders are known, in which the common starting point is gluten-induced zonulin release. Zonulin results in varying degrees of increased permeability in certain gluten-related disorders but is largely responsible for the development of further pathogenic processes and symptoms. Therefore, it is important to know the barrier-modulating role of individual nutritional components and to what extent the antioxidant substance supports the protection of gliadin-induced membrane damage with its radical scavenging capacity. We investigated the pH dependence of the gliadin-anthocyanin interaction using UV photometry, during which a concentration-dependent interaction was observed at pH 6.8. The barrier modulatory effect of the anthocyanin-rich sour cherry extract (AC) was analyzed on Caco-2 cell culture with pepsin-trypsin-resistant gliadin (PT-gliadin) exposure by TEER measurement, zonula occludens-1 (ZO-1), and Occludin immunohistochemistry. In addition to the TEER-reducing and TJ-rearranging effects of PT-gliadin, NF-κB activation, an increase in cytokine (TNF-α, IFN-γ, and IL-8) release, and mitochondrial ROS levels were observed. We confirmed the anti-inflammatory, stabilizing, and restoring roles of AC extract during gliadin treatment on the Caco-2 monolayer. The extract was able to significantly reduce cytokine and ROS levels despite the known interaction of the main components of the extract with PT-gliadin. LA - English DB - MTMT ER - TY - JOUR AU - Erdélyi, Lóránd AU - Váradi, Judit TI - Lokális antibiotikum-felhasználás csökkentése, illetve kiváltása sebkezelésben JF - GYÓGYSZERÉSZET J2 - GYÓGYSZERÉSZET VL - 67 PY - 2023 SP - 199 EP - 202 PG - 4 SN - 0017-6036 UR - https://m2.mtmt.hu/api/publication/33770210 ID - 33770210 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Skopkó, Boglárka Emese AU - Paholcsek, Melinda AU - Szilágyi-Rácz, Anna Anita AU - Fauszt, Péter AU - Dávid, Péter AU - Stündl, László AU - Váradi, Judit AU - Kovács, Renátó AU - Bágyi, Kinga, Ágnes AU - Gálné Remenyik, Judit TI - High-Throughput Sequencing Analysis of the Changes in the Salivary Microbiota of Hungarian Young and Adult Subpopulation by an Anthocyanin Chewing Gum and Toothbrush Change JF - DENTISTRY JOURNAL J2 - DENT J-BASEL VL - 11 PY - 2023 IS - 2 PG - 16 SN - 2304-6767 DO - 10.3390/dj11020044 UR - https://m2.mtmt.hu/api/publication/33637085 ID - 33637085 AB - The sour cherry contains anthocyanins, which have bactericide action against some oral bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa). Sour cherry also has antibiofilm action against Streptococcus mutans, Candida albicans, and Fusobacterium nucleatum. Our earlier research proved that chewing sour cherry anthocyanin gum significantly reduces the amount of human salivary alpha-amylase and Streptococcus mutans levels. The microbiota of a toothbrush affects oral health and regular toothbrush change is recommended. A total of 20 healthy participants were selected for the study. We analysed saliva samples with 16S rRNA sequencing to investigate the effect of 2 weeks (daily three times, after main meals) of chewing sour cherry anthocyanin gum—supplemented by toothbrush change in half of our case–control study cohort—after scaling on human oral microbiota. A more stable and diverse microbiome could be observed after scaling by the anthocyanin gum. Significant differences between groups (NBR: not toothbrush changing; BR: toothbrush changing) were evaluated by log2 proportion analysis of the most abundant family and genera. The analysis showed that lower level of some Gram-negative anaerobic (Prevotella melaninogenica, Porphyromonas pasteri, Fusobacterium nucleatum subsp. vincentii) and Gram-positive (Rothia mucilaginosa) bacteria could be observed in the case group (BR), accompanied by build-up of health-associated Streptococcal network connections. LA - English DB - MTMT ER - TY - JOUR AU - Demeter, Fruzsina AU - Török, Patrik AU - Kiss, Alexandra AU - Kovásznai-Oláh, Richárd AU - Máthéné Szigeti, Zsuzsa AU - Baksa, Viktória AU - Kovács, Fruzsina AU - Balla , Noémi AU - Fenyvesi, Ferenc AU - Váradi, Judit AU - Borbás, Anikó AU - Herczeg, Mihály TI - First Synthesis of DBU-Conjugated Cationic Carbohydrate Derivatives and Investigation of Their Antibacterial and Antifungal Activity JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 4 PG - 20 SN - 1661-6596 DO - 10.3390/ijms24043550 UR - https://m2.mtmt.hu/api/publication/33635459 ID - 33635459 AB - The emergence of drug-resistant bacteria and fungi represents a serious health problem worldwide. It has long been known that cationic compounds can inhibit the growth of bacteria and fungi by disrupting the cell membrane. The advantage of using such cationic compounds is that the microorganisms would not become resistant to cationic agents, since this type of adaptation would mean significantly altering the structure of their cell walls. We designed novel, DBU (1,8-diazabicyclo[5.4.0]undec-7-ene)-derived amidinium salts of carbohydrates, which may be suitable for disturbing the cell walls of bacteria and fungi due to their quaternary ammonium moiety. A series of saccharide-DBU conjugates were prepared from 6-iodo derivatives of d-glucose, d-mannose, d-altrose and d-allose by nucleophilic substitution reactions. We optimized the synthesis of a d-glucose derivative, and studied the protecting group free synthesis of the glucose-DBU conjugates. The effect of the obtained quaternary amidinium salts against Escherichia coli and Staphylococcus aureus bacterial strains and Candida albicans yeast was investigated, and the impact of the used protecting groups and the sugar configuration on the antimicrobial activity was analyzed. Some of the novel sugar quaternary ammonium compounds with lipophilic aromatic groups (benzyl and 2-napthylmethyl) showed particularly good antifungal and antibacterial activity. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Renátó AU - Erdélyi, Lóránd AU - Fenyvesi, Ferenc AU - Balla , Noémi AU - Kovács, Fruzsina AU - Vámosi, György AU - Klusóczki, Ágnes AU - Gyöngyösi, Alexandra AU - Bácskay, Ildikó AU - Vecsernyés, Miklós AU - Váradi, Judit TI - Concentration-Dependent Antibacterial Activity of Chitosan on Lactobacillus plantarum JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 1 SN - 1999-4923 DO - 10.3390/pharmaceutics15010018 UR - https://m2.mtmt.hu/api/publication/33434625 ID - 33434625 AB - The antimicrobial effect of chitosan and synthetic chitosan derivatives has been confirmed on many Gram-positive and Gram-negative bacteria and fungi. The tests were carried out on pathogenic microorganisms, so the mechanism and concentration dependence of the inhibitory effect of chitosan were revealed. We conducted our tests on a probiotic strain, Lactobacillus plantarum. Commercially available chitosan derivatives of different molecular weights were added to L. plantarum suspension in increasing concentrations. The minimum inhibitory concentration (MIC) value of chitosan was determined and confirmed the viability decreasing effect at concentrations above the MIC with a time-kill assay. The release of bacterium cell content was measured at 260 nm after treatment with 0.001–0.1% concentration chitosan solution. An increase in the permeability of the cell membrane was observed only with the 0.1% treatment. The interaction was also investigated by zeta potential measurement, and the irreversible interaction and concentration dependence were established in all concentrations. The interaction of fluorescein isothiocyanate (FITC) labeled low molecular weight chitosan and bacterial cells labeled with membrane dye (FM® 4–64) was confirmed by confocal microscopy. In conclusion, the inhibitory effect of chitosan was verified on a probiotic strain, which is an undesirable effect in probiotic preparations containing chitosan additives, while the inhibitory effect experienced with pathogenic strains is beneficial. LA - English DB - MTMT ER - TY - JOUR AU - Balog, Katalin Ágnes AU - Váradi, Judit TI - Az insomnia terápiás lehetőségei a gyógyszerészi gondozás keretein belül JF - GYÓGYSZERÉSZET J2 - GYÓGYSZERÉSZET VL - 66 PY - 2022 SP - 410 EP - 419 PG - 10 SN - 0017-6036 UR - https://m2.mtmt.hu/api/publication/34048178 ID - 34048178 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Trotta, Maria Consiglia AU - Petrillo, Francesco AU - Gesualdo, Carlo AU - Rossi, Settimio AU - Corte, Alberto Della AU - Váradi, Judit AU - Fenyvesi, Ferenc AU - D’Amico, Michele AU - Hermenean, Anca TI - Effects of the Calix[4]arene Derivative Compound OTX008 on High Glucose-Stimulated ARPE-19 Cells: Focus on Galectin-1/TGF-β/EMT Pathway JF - MOLECULES J2 - MOLECULES VL - 27 PY - 2022 IS - 15 SN - 1420-3049 DO - 10.3390/molecules27154785 UR - https://m2.mtmt.hu/api/publication/33338835 ID - 33338835 AB - Diabetic retinopathy (DR) is a neurovascular disease characterized by the reduction of retina integrity and functionality, as a consequence of retinal pigment epithelial cell fibrosis. Although galectin-1 (a glycan-binding protein) has been associated with dysregulated retinal angiogenesis, no evidence has been reported about galectin-1 roles in DR-induced fibrosis. ARPE-19 cells were cultured in normal (5 mM) or high glucose (35 mM) for 3 days, then exposed to the selective galectin-1 inhibitor OTX008 (2.5–5–10 μM) for 6 days. The determination of cell viability and ROS content along with the analysis of specific proteins (by immunocytochemistry, Western blotting, and ELISA) or mRNAs (by real time-PCR) were performed. OTX008 5 μM and 10 μM improved cell viability and markedly reduced galectin-1 protein expression in cells exposed to high glucose. This was paralleled by a down-regulation of the TGF-β/, NF-kB p65 levels, and ROS content. Moreover, epithelial–mesenchymal transition markers were reduced by OTX008 5 μM and 10 μM. The inhibition of galectin-1 by OTX008 in DR may preserve retinal pigment epithelial cell integrity and functionality by reducing their pro-fibrotic phenotype and epithelial–mesenchymal transition phenomenon induced by diabetes. LA - English DB - MTMT ER -