TY - JOUR AU - Rani, Vaishali AU - Shetty, Prateek AU - Maróti, Gergely TI - Comparative transcriptome study highlights the versatility of nitrogen metabolism in Chlamydomonas JF - ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS J2 - ALGAL RES VL - 79 PY - 2024 SP - 1 EP - 13 PG - 13 SN - 2211-9264 DO - 10.1016/j.algal.2024.103458 UR - https://m2.mtmt.hu/api/publication/34764951 ID - 34764951 AB - Nitrogen is an essential macronutrient and nitrate is one of the main forms of this macronutrient available for plants and microbes. Nitrate is not only the substrate for the nitrate assimilation pathway, but also a crucial signal for the regulation of numerous metabolic, developmental, and cellular differentiation processes. In the present study, two species of the Chlamydomonas genus, Chlamydomonas reinhardtii cc124 and Chlamydomonas sp. MACC-216 were used to investigate the versatility of nitrate metabolism in green microalgae. Quantification of nitrate removal efficiency showed that Chlamydomonas sp. MACC-216 strongly outperforms C. reinhardtii cc124. Transcriptional changes occurring under nitrate-replete and nitrate-deplete conditions were specifically investigated in the selected species of Chlamydomonas. Whole transcriptome analysis revealed that the genes playing a role in nitrate assimilation did not show differential expression in C. reinhardtii cc124 under changing nitrate conditions (only 45 genes exhibited differential regulation), while in Chlamydomonas sp. MACC-216 a large set of genes (3143) showed altered expression. Furthermore, genes responsible for urea metabolism, like DUR3A gene corresponding to urea transport, were found to be upregulated in Chlamydomonas sp. MACC-216 under nitrate-deplete condition, while the same gene showed elevated expression level in C. reinhardtii cc124 under nitrate-replete condition. The present study indicated the diverseness of nitrate metabolism among species within the Chlamydomonas genus. LA - English DB - MTMT ER - TY - JOUR AU - Papp, Márton AU - Tóth, Adrienn Gréta AU - Békési, László AU - Farkas, Róbert László AU - Makrai, László AU - Maróti, Gergely AU - Solymosi, Norbert TI - Apis mellifera filamentous virus from a honey bee gut microbiome survey in Hungary JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 SP - 1 EP - 8 PG - 8 SN - 2045-2322 DO - 10.1038/s41598-024-56320-x UR - https://m2.mtmt.hu/api/publication/34730183 ID - 34730183 N1 - Funding Agency and Grant Number: European Union [874735]; Hungarian Academy of Sciences [LP2020-5/2020]; NKP-22-3-II. New National Excellence program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund; University of Veterinary Medicine Funding text: In memory of Rajnald Andras Koveshegyi OCist. We would like to say thanks to the beekeepers for giving us their indispensable help. It has also received funding from the European Union's Horizon 2020 research and innovation program under Grant Agreement No. 874735 (VEO). GM received support from the Hungarian Academy of Sciences through the Lenduelet-Programme (LP2020-5/2020). Supported by the & Uacute;NKP-22-3-II. New National Excellence program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund.Open access funding provided by University of Veterinary Medicine. AB - In Hungary, as part of a nationwide, climatically balanced survey for a next-generation sequencing-based study of the honey bee (Apis mellifera) gut microbiome, repeated sampling was carried out during the honey production season (March and May 2019). Among other findings, the presence of Apis mellifera filamentous virus (AmFV) was detected in all samples, some at very high levels. AmFV-derived reads were more abundant in the March samples than in the May samples. In March, a higher abundance of AmFV-originated reads was identified in samples collected from warmer areas compared to those collected from cooler areas. A lower proportion of AmFV-derived reads were identified in samples collected in March from the wetter areas than those collected from the drier areas. AmFV-read abundance in samples collected in May showed no significant differences between groups based on either environmental temperature or precipitation. The AmFV abundance correlated negatively with Bartonella apihabitans, Bartonella choladocola, and positively with Frischella perrara, Gilliamella apicola, Gilliamella sp. ESL0443, Lactobacillus apis, Lactobacillus kullabergensis, Lactobacillus sp. IBH004. De novo metagenome assembly of four samples resulted in almost the complete AmFV genome. According to phylogenetic analysis based on DNA polymerase, the Hungarian strains are closest to the strain CH-05 isolated in Switzerland. LA - English DB - MTMT ER - TY - JOUR AU - Papp, Márton AU - Békési, László AU - Farkas, Róbert László AU - Makrai, László AU - Maróti, Gergely AU - Solymosi, Norbert TI - Vairimorpha (Nosema) ceranae előfordulása Magyarországon, mézelő méhek (Apis mellifera) bélmikrobiom-felmérése alapján JF - MAGYAR ÁLLATORVOSOK LAPJA J2 - MAGY ALLATORVOSOK VL - 146 PY - 2024 IS - 3 SP - 149 EP - 159 PG - 11 SN - 0025-004X DO - 10.56385/magyallorv.2024.03.149-159 UR - https://m2.mtmt.hu/api/publication/34728404 ID - 34728404 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Vályi, Péter AU - Wirth, Roland AU - Minárovits, János AU - Strang, Orsolya AU - Maróti, Gergely AU - Kovács, Kornél Lajos TI - The oral microbiome of a family including Papillon-Lefèvre-syndrome patients and clinically healthy members JF - BMC ORAL HEALTH J2 - BMC ORAL HEALTH VL - 24 PY - 2024 IS - 1 PG - 17 SN - 1472-6831 DO - 10.1186/s12903-024-03856-z UR - https://m2.mtmt.hu/api/publication/34558800 ID - 34558800 N1 - Department of Oral Diagnostics, Faculty of Dentistry, Semmelweis University, Szentkirályi u 47, Budapest, H1085, Hungary Department of Biotechnology, University of Szeged, Közép fasor 52, Szeged, H6726, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Tisza L. krt 64, Szeged, H6720, Hungary Institute of Biophysics, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Institute of Plant Biology, Biological Research Center, Temesvári krt 62, Szeged, H6726, Hungary Export Date: 1 May 2024 Correspondence Address: Vályi, P.; Department of Oral Diagnostics, Szentkirályi u 47, Hungary; email: valyi.peter@dent.semmelweis-univ.hu Chemicals/CAS: dipeptidyl peptidase I, 9032-68-2 Funding details: European Regional Development Fund, ERDF, FK123899, GINOP-2.3.2-15-2016-00011, PD132145 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding text 1: This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. Funding text 2: Open access funding provided by Semmelweis University. This work was supported by the European Regional Development Fund to a project led by JM; grant No.: GINOP-2.3.2-15-2016-00011. RW (PD132145) and GM (FK123899) received support from the National Research, Development and Innovation Office (NKFIH), Hungary. AB - The oral microbiota composition of patients diagnosed with Papillon-Lefèvre-syndrome and treated for several years were compared to those existing in the oral cavity of the clinically healthy family members and a cohort of patients having various stages of chronic periodontitis. LA - English DB - MTMT ER - TY - JOUR AU - Stirk, Wendy A. AU - Bálint, Péter AU - Široká, Jitka AU - Novák, Ondřej AU - Rétfalvi, Tamás AU - Berzsenyi, Zoltán AU - Notterpek T., Jácint AU - Varga, Zoltán AU - Maróti, Gergely AU - van Staden, Johannes AU - Strnad, Miroslav AU - Ördög, Vince TI - Comparison of plant biostimulating properties of Chlorella sorokiniana biomass produced in batch and semi-continuous systems supplemented with pig manure or acetate JF - JOURNAL OF BIOTECHNOLOGY J2 - J BIOTECHNOL VL - 381 PY - 2024 SP - 27 EP - 35 PG - 9 SN - 0168-1656 DO - 10.1016/j.jbiotec.2024.01.002 UR - https://m2.mtmt.hu/api/publication/34498476 ID - 34498476 N1 - Funding Agency and Grant Number: European Union [727874]; Lendulet-Programme of the Hungarian Academy of Sciences [LP2020-5/2020]; Czech Grant Agency [19-00973S]; European Regional Development Fund Project [CZ.02.1.01/0.0/0.0/16_019/0000827]; University of KwaZulu-Natal Funding text: The research was funded by the project SABANA (grant number 727874) from the European Union Horizon 2020 Research and Innovation Program (VO) and the Lendulet-Programme of the Hungarian Academy of Sciences (LP2020-5/2020; GM) . This research was also supported by the Czech Grant Agency (No. 19-00973S) and European Regional Development Fund Project "Plants as a tool for sustainable global development" No. CZ.02.1.01/0.0/0.0/16_019/0000827) . The University of KwaZulu-Natal is also thanked for financial support. AB - Microalgae-derived biostimulants provide an eco-friendly biotechnology for improving crop productivity. The strategy of circular economy includes reducing biomass production costs of new and robust microalgae strains grown in nutrient-rich wastewater and mixotrophic culture where media is enriched with organic carbon. In this study, Chlorella sorokiniana was grown in 100 l bioreactors under sub-optimal conditions in a greenhouse. A combination of batch and semi-continuous cultivation was used to investigate the growth, plant hormone and biostimulating effect of biomass grown in diluted pig manure and in nutrient medium supplemented with Naacetate. C. sorokiniana tolerated the low light (sum of PAR 0.99 ± 0.18 mol/photons/(m2 /day)) and temperature (3.7–23.7◦ C) conditions to maintain a positive growth rate and daily biomass productivity (up to 149 mg/l/ day and 69 mg/l/day dry matter production in pig manure and Na-acetate supplemented cultures respectively). The protein and lipid content was significantly higher in the biomass generated in batch culture and dilute pig manure (1.4x higher protein and 2x higher lipid) compared to the Na-acetate enriched culture. Auxins indole-3- acetic acid (IAA) and 2-oxindole-3-acetic acid (oxIAA) and salicylic acid (SA) were present in the biomass with significantly higher auxin content in the biomass generated using pig manure (> 350 pmol/g DW IAA and > 84 pmol/g DW oxIAA) compared to cultures enriched with Na-acetate and batch cultures (< 200 pmol/g DW IAA and < 27 pmol/g DW oxIAA). No abscisic acid and jasmonates were detected. All samples had plant biostimulating activity measured in the mungbean rooting bioassay with the Na-acetate supplemented biomass eliciting higher rooting activity (equivalent to 1–2 mg/l IBA) compared to the pig manure (equivalent to 0.5–1 mg/l IBA) and batch culture (equivalent to water control) generated biomass. Thus C. sorokiniana MACC728 is a robust new strain for biotechnology, tolerating low light and temperature conditions. The strain can adapt to alternative nutrient (pig manure) and carbon (acetate) sources with the generated biomass having a high auxin concentration and plant biostimulating activity detected with the mungbean rooting bioassay. LA - English DB - MTMT ER - TY - JOUR AU - Zlatkova Petrova, Nia AU - Tóth, Tünde AU - Shetty, Prateek AU - Maróti, Gergely AU - Tóth, Szilvia Zita TI - Enhancing biophotovoltaic efficiency: Study on a highly productive green algal strain Parachlorella kessleri MACC-38 JF - BIORESOURCE TECHNOLOGY J2 - BIORESOUR TECHNOL VL - 394 PY - 2024 SP - 130206 SN - 0960-8524 DO - 10.1016/j.biortech.2023.130206 UR - https://m2.mtmt.hu/api/publication/34493118 ID - 34493118 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Office [PD143438, K132600]; Lenduelet-Programme of the Hungarian Academy of Sciences [LP2020-5/2020]; European Union [892632] Funding text: This work was supported by the National Research, Development, and Innovation Office (PD143438 and K132600, research grants to N. Z. P. and S.Z.T.), by the Lenduelet-Programme of the Hungarian Academy of Sciences (LP2020-5/2020 to G.M.), and by the European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie grant agreement No 892632 (EnergUP project, to T.N. T.). AB - Biophotovoltaic (BPV) devices are a potential decentralized and environmentally friendly energy source that harness solar energy through photosynthesis. BPV devices are self-regenerating, promising long-term usability. A practical strategy for enhancing BPV performance is to systematically screen for highly exoelectrogenic algal strains capable of generating large electric current density. In this study, a previously uncharacterized green algal strain -Parachlorella kessleri MACC-38 was found to generate over 340 mu A mg � 1 Chl cm-2. This output is approximately ten-fold higher than those of Chlamydomonas reinhardtii and Chlorella species. The current production of MACC-38 primarily originates from photosynthesis, and the strain maintains its physiological integrity throughout the process. MACC-38 exhibits unique traits such as low extracellular O2 and Fe(III) reduction, substantial copper (II) reduction, and significant extracellular acidification during current generation, contributing to its high productivity. The exoelectrogenic and growth characteristics of MACC-38 suggest that it could markedly boost BPV efficiency. LA - English DB - MTMT ER - TY - JOUR AU - Junga, Anna AU - Pilmane, Māra AU - Šerstņova, Ksenija AU - Lohova, Elizabeta AU - Melderis, Ivars AU - Gontar, Łukasz AU - Kochański, Maksymilian AU - Drutowska, Andżelika AU - Maróti, Gergely AU - Prieto-Simón, Beatriz TI - Composition of Mastitis Causing Microorganisms and Cytokines in Healthy Cow’s Milk: A Pilot Study JF - PROCEEDINGS OF THE LATVIAN ACADEMY OF SCIENCES SECTION B: NATURAL EXACT AND APPLIED SCIENCES J2 - PROC LATVIAN ACAD SCI SECT B VL - 77 PY - 2023 IS - 3-4 SP - 169 EP - 177 PG - 9 SN - 1407-009X DO - 10.2478/prolas-2023-0024 UR - https://m2.mtmt.hu/api/publication/34750600 ID - 34750600 AB - The aim of this study was to examine clinically healthy cow’s udder milk microbiota and presence of cytokines in different seasons. Milk samples taken from the cows were checked for the presence of Gram-positive and Gram-negative bacteria, and the somatic cell count was detected. Immunohistochemistry methods were performed to detect interleukin (IL) -2, IL-4, IL-8, IL-10, IL-12, IL-17a, β-defensin-3, transforming growth factor (TGF)-ā1, interferon-ć and nuclear factor (NF)-źB presence in the milk. S. agalactiae, S. uberis, S. aureus, E. coli, and Klebsiella, Enterobacter, Citrobacter spp. were found in healthy cow’s milk. In the first round, the highest prevalence was observed for S. aureus . In the second round, the highest mean levels were observed for S. uberis , then followed S. aureus . IL-4, IL-17a and TGF-ā1 demonstrated the highest expression in the milk samples. NF-źB had the lowest expression among all factors. The presence of a rich bacterial microbiome (mostly S.aureus , S.uberis ) in the milk of healthy animals, as well as changing bacterial species between in spring and autumn seasons occur as a result of both the immune state of the animal and many external factors, which consequently affects the amount of expressed cytokines. LA - English DB - MTMT ER - TY - JOUR AU - Xu, Xinming AU - Nielsen, Lasse Johan Dyrbye AU - Song, Lijie AU - Maróti, Gergely AU - Strube, Mikael Lenz AU - Kovács, Ákos T. TI - Enhanced specificity of Bacillus metataxonomics using a tuf-targeted amplicon sequencing approach JF - ISME COMMUNICATIONS J2 - ISME COMMUN VL - 3 PY - 2023 IS - 1 SN - 2730-6151 DO - 10.1038/s43705-023-00330-9 UR - https://m2.mtmt.hu/api/publication/34489404 ID - 34489404 N1 - Funding Agency and Grant Number: Chinese Scholarship Council; Lenduelet-Programme of the Hungarian Academy of Sciences [LP2020-5/2020]; Danish National Research Foundation for the Center for Microbial Secondary Metabolites [DNRF137]; Novo Nordisk Foundation within the INTERACT project of the Collaborative Crop Resiliency Program [NNF19SA0059360] Funding text: XX was supported by a Chinese Scholarship Council fellowship. GM was supported by the Lenduelet-Programme of the Hungarian Academy of Sciences (LP2020-5/2020). This project was supported by the Danish National Research Foundation (DNRF137) for the Center for Microbial Secondary Metabolites and the Novo Nordisk Foundation within the INTERACT project of the Collaborative Crop Resiliency Program (NNF19SA0059360). AB - Bacillus species are ubiquitous in nature and have tremendous application potential in agriculture, medicine, and industry. However, the individual species of this genus vary widely in both ecological niches and functional phenotypes, which, hence, requires accurate classification of these bacteria when selecting them for specific purposes. Although analysis of the 16S rRNA gene has been widely used to disseminate the taxonomy of most bacterial species, this gene fails proper classification of Bacillus species. To circumvent this restriction, we designed novel primers and optimized them to allow exact species resolution of Bacillus species in both synthetic and natural communities using high-throughput amplicon sequencing. The primers designed for the tuf gene were not only specific for the Bacillus genus but also sufficiently discriminated species both in silico and in vitro in a mixture of 11 distinct Bacillus species. Investigating the primers using a natural soil sample, 13 dominant species were detected including Bacillus badius , Bacillus velezensis , and Bacillus mycoides as primary members, neither of which could be distinguished with 16S rRNA sequencing. In conclusion, a set of high-throughput primers were developed which allows unprecedented species-level identification of Bacillus species and aids the description of the ecological distribution of Bacilli in various natural environment. LA - English DB - MTMT ER - TY - GEN AU - Patyi, Gábor AU - Hódi, Barbara AU - Mallick, Ivy AU - Maróti, Gergely AU - Kós, Péter AU - Vass, Imre TI - Investigation of singlet oxygen sensitive genes in the cyanobacterium Synechocystis PCC 6803 PY - 2023 UR - https://m2.mtmt.hu/api/publication/34477263 ID - 34477263 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Adrienn Gréta AU - Farkas, Róbert László AU - Papp, Márton AU - Kilim, Oz Sam AU - Yun, H. AU - Makrai, László AU - Maróti, Gergely AU - Gyurkovszky, Mónika AU - Krikó, Eszter AU - Solymosi, Norbert TI - Ixodes ricinus tick bacteriome alterations based on a climatically representative survey in Hungary JF - MICROBIOLOGY SPECTRUM J2 - MICROBIOL SPEC VL - 11 PY - 2023 IS - 6 SP - 1 EP - 17 PG - 17 SN - 2165-0497 DO - 10.1128/spectrum.01243-23 UR - https://m2.mtmt.hu/api/publication/34471601 ID - 34471601 N1 - Centre for Bioinformatics, University of Veterinary Medicine, Budapest, Hungary Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary Department of Physics of Complex Systems, Eötvös Loránd University, Budapest, Hungary Budapest, Hungary Institute of Plant Biology, Biological Research Center, Szeged, Hungary Faculty of Water Sciences, University of Public Service, Baja, Hungary Export Date: 02 January 2024; Cited By: 0 AB - The microbial communities of disease vectors may represent a key feature in several biological functions and thus deserve special attention in light of climate change and the consequent need to develop novel control strategies. Nevertheless, vector-borne microbial networks are still poorly understood. Assessing vectors’ microbial interactions and climatic dependencies may contribute to better estimating pathogen transmission characteristics and public health risks. In a climatically representative country-wide survey, Ixodes ricinus ticks were collected from 17 locations in Hungary. Using shotgun metagenomic sequencing, the bacteriome composition was analyzed by investigating the relationship between the abundance of nymphs and females in various climatic environments. Bacterial composition on the genus level revealed a significant difference between the samples from females and nymphs. Within the core bacteriome, females and nymphs showed significant variation in the following genera: Arsenophonus, Bacillus, Candidatus Midichloria, Rhodococcus, Sphingomonas, Staphylococcus, and Wolbachia. Among females, according to temperature strata, the following were found differentiating: Curtobacterium, Pseudomonas, and Sphingomonas. There was no genus with a significant difference in precipitation categories for females. Curtobacterium showed significant variation between temperature and Bacillus and Curtobacterium for various precipitation levels in the nymphs. The composition of vector-borne bacteriome members showed significant alterations at sampling points with different climatic conditions and development stages of the tick hosts. Our findings not only pave the way toward understanding tick-borne bacterial networks and interdependencies but also shed light on the high potential for the presence of a possible biological tick control species, the tick parasitoid, Ixodiphagus hookeri based on related bacteriome patterns. LA - English DB - MTMT ER -