@article{MTMT:34763514,
	title = {Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development},
	url = {https://m2.mtmt.hu/api/publication/34763514},
	author = {Gödöny, Krisztina and Herczeg, Róbert and Gyenesei, Attila and Várnagy, Ákos and Bognár, Zoltán and Kovács, L. Gábor and Szekeres, Júlia and Mauchart, Péter and Nagy, Bernadett and Erostyák, János and Kovács, Kálmán András and Bódis, József},
	doi = {10.1159/000536358},
	journal-iso = {MED PRIN PRACT},
	journal = {MEDICAL PRINCIPLES AND PRACTICE},
	unique-id = {34763514},
	issn = {1011-7571},
	abstract = {<b><i>Objective:</i></b> Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). <b><i>Materials and Methods:</i></b> We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. <b><i>Results:</i></b> Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. <b><i>Conclusions:</i></b> The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety.},
	keywords = {blastocyst; abortion; intracytoplasmic sperm injection; In vitro fertilization; Live birth rate; light protection},
	year = {2024},
	eissn = {1423-0151},
	orcid-numbers = {Herczeg, Róbert/0000-0002-5903-0082; Kovács, L. Gábor/0000-0001-5298-5401}
}

@article{MTMT:34689825,
	title = {Cyclodextrin derivatives decrease Transient Receptor Potential vanilloid 1 and Ankyrin 1 ion channel activation via altering the surrounding membrane microenvironment by cholesterol depletion},
	url = {https://m2.mtmt.hu/api/publication/34689825},
	author = {Nehr-Majoros, Andrea Kinga and Erostyák, János and Fenyvesi, Éva and Szabó-Meleg, Edina and Szőcs, Levente and Sétáló, György (ifj.) and Helyes, Zsuzsanna and Szőke, Éva},
	doi = {10.3389/fcell.2024.1334130},
	journal-iso = {FRONT CELL DEV BIOL},
	journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY},
	volume = {12},
	unique-id = {34689825},
	issn = {2296-634X},
	abstract = {Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) are nonselective cation channels expressed in primary sensory neurons and several other non-neuronal structures such as immune cells, keratinocytes, and vascular smooth muscle cells. They play important roles in nociception, pain processing and their chanellopathies are associated with the development of several pathological conditions. They are located in cholesterol- and sphingolipid-rich membrane lipid raft regions serving as platforms to modulate their activations. We demonstrated earlier that disruption of these lipid rafts leads to decreased TRP channel activation and exerts analgesic effects. Cyclodextrins are macrocyclic molecules able to form host-guest complexes with cholesterol and deplete it from the membrane lipid rafts. The aim of this study was to investigate 8 structurally different (methylated and non-methylated) CD derivatives on cell viability, mitochondrial membrane potential, membrane composition and activation abilities of the TRPV1 and TRPA1 channels. We showed that non-methylated derivatives have preferable safety profiles compared to methylated ones. Furthermore, methylated derivatives reduced mitochondrial membrane potential. However, all investigated derivatives influence the ordered cell membrane structure depleting membrane cholesterol and inhibit the TRPV1 agonist capsaicin- and the TRPA1 agonist allyl isothiocyanate-induced Ca 2+− influx. This mechanism of action might provide novel perspectives for the development of peripherally acting analgesics via indirectly decreasing the generation and transmission of nociceptive signals.},
	year = {2024},
	eissn = {2296-634X},
	orcid-numbers = {Szőcs, Levente/0000-0002-9521-3628}
}

@article{MTMT:34212239,
	title = {Lipid raft disruption affects membrane fluidity in CHO cells and Transient Receptor Potential Melastatin 8 ion channel activation in in vivo mouse model},
	url = {https://m2.mtmt.hu/api/publication/34212239},
	author = {Szoke, Eva and Horvath, Adam and Nehr-Majoros, Andrea and Berenkei, Gabor and Erostyák, János and Helyes, Zsuzsanna},
	journal-iso = {BR J PHARMACOL},
	journal = {BRITISH JOURNAL OF PHARMACOLOGY},
	volume = {180},
	unique-id = {34212239},
	issn = {0007-1188},
	year = {2023},
	eissn = {1476-5381},
	pages = {1011-1012}
}

@article{MTMT:33219649,
	title = {Effect of Lipid Raft Disruptors on Cell Membrane Fluidity Studied by Fluorescence Spectroscopy},
	url = {https://m2.mtmt.hu/api/publication/33219649},
	author = {Horváth, Ádám and Erostyák, János and Szőke, Éva},
	doi = {10.3390/ijms232213729},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {23},
	unique-id = {33219649},
	issn = {1661-6596},
	abstract = {Lipid rafts are specialized microdomains in cell membranes, rich in cholesterol and sphingolipids, and play an integrative role in several physiological and pathophysiological processes. The integrity of rafts can be disrupted via their cholesterol content—with methyl-β-cyclodextrin (MCD) or with our own carboxamido-steroid compound (C1)—or via their sphingolipid content—with sphingomyelinase (SMase) or with myriocin (Myr). We previously proved by the fluorescent spectroscopy method with LAURDAN that treatment with lipid raft disruptors led to a change in cell membrane polarity. In this study, we focused on the alteration of parameters describing membrane fluidity, such as generalized polarization (GP), characteristic time of the GP values change—Center of Gravity (τCoG)—and rotational mobility (τrot) of LAURDAN molecules. Myr caused a blue shift of the LAURDAN spectrum (higher GP value), while other agents lowered GP values (red shift). MCD decreased the CoG values, while other compounds increased it, so MCD lowered membrane stiffness. In the case of τrot, only Myr lowered the rotation of LAURDAN, while the other compounds increased the speed of τrot, which indicated a more disordered membrane structure. Overall, MCD appeared to increase the fluidity of the membranes, while treatment with the other compounds resulted in decreased fluidity and increased stiffness of the membranes.},
	year = {2022},
	eissn = {1422-0067}
}

@article{MTMT:31848850,
	title = {Analgesic Effects of Lipid Raft Disruption by Sphingomyelinase and Myriocin via Transient Receptor Potential Vanilloid 1 and Transient Receptor Potential Ankyrin 1 Ion Channel Modulation},
	url = {https://m2.mtmt.hu/api/publication/31848850},
	author = {Horváth, Ádám and Payrits, Maja and Steib, Anita and Kántás, Boglárka and Biró-Sütő, Tünde and Erostyák, János and Makkai, Géza and Sághy, Éva and Helyes, Zsuzsanna and Szőke, Éva},
	doi = {10.3389/fphar.2020.593319},
	journal-iso = {FRONT PHARMACOL},
	journal = {FRONTIERS IN PHARMACOLOGY},
	volume = {11},
	unique-id = {31848850},
	year = {2021},
	eissn = {1663-9812},
	orcid-numbers = {Sághy, Éva/0000-0002-4031-3461}
}

@article{MTMT:31677101,
	title = {How to reduce the potential harmful effects of light on blastocyst development during IVF},
	url = {https://m2.mtmt.hu/api/publication/31677101},
	author = {Bódis, József and Gödöny, Krisztina and Várnagy, Ákos and Kovács, Kálmán András and Koppán, Miklós and Nagy, Bernadett and Erostyák, János and Herczeg, Róbert and Szekeres, Júlia and Gyenesei, Attila and Kovács, L. Gábor},
	doi = {10.1159/000509016},
	journal-iso = {MED PRIN PRACT},
	journal = {MEDICAL PRINCIPLES AND PRACTICE},
	volume = {29},
	unique-id = {31677101},
	issn = {1011-7571},
	abstract = {Earlier findings revealed the damaging effect of visible light on zygotes and gametes. The aim of our study is to eliminate or significantly reduce the potential harmful effect of light exposure during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and to investigate the effect of light protection on embryo development and implantation.To protect sperm cells, oocytes and embryos from the potential harmful effects of light exposure during laboratory procedures, we created a dark environment for the cells and applied red filters on laboratory lamps and UV or infrared filters in the microscopes - to eliminate white light exposure of the cells throughout all work stages.The fertilization rate was significantly (p = 0.011) higher in light protected ICSI cycles. Blastocyst development rates (blastocyst/embryo) were significantly (p<0.001) higher in light protected embryos than in those manipulated in conventional light conditions both in IVF (20.9% difference) and ICSI (38.6% difference). Number of clinical pregnancies/transfers of ICSI fertilized day 5 blastocysts were significantly (p=0.040) higher in light protected conditions.This data show that light protection has a positive effect on fertilization rate and increases the blastocyst development as well as number of clinical pregnancies/transfers. Implementation of this light protection method in IVF centers may improve the success rate while maintaining maximal embryo safety.},
	year = {2020},
	eissn = {1423-0151},
	pages = {558-564},
	orcid-numbers = {Herczeg, Róbert/0000-0002-5903-0082; Kovács, L. Gábor/0000-0001-5298-5401}
}

@article{MTMT:31625361,
	title = {In vitro and in vivo evidence for the role of lipid rafts in Ca2+-gating of the Transient Receptor Potential channels in sensory neurons},
	url = {https://m2.mtmt.hu/api/publication/31625361},
	author = {Szőke, Éva and Horvath, Adam and Biró-Sütő, Tünde and Sághy, Éva and Payrits, Maja and Erostyák, János and Makkai, Géza and Szolcsányi, János and Helyes, Zsuzsanna},
	doi = {10.1096/fasebj.2020.34.s1.05167},
	journal-iso = {FASEB J},
	journal = {FASEB JOURNAL},
	volume = {34},
	unique-id = {31625361},
	issn = {0892-6638},
	keywords = {Biology; Biochemistry & Molecular Biology},
	year = {2020},
	eissn = {1530-6860},
	orcid-numbers = {Sághy, Éva/0000-0002-4031-3461}
}

@article{MTMT:31387590,
	title = {Resolvin D1 and D2 inhibit transient receptor potential vanilloid 1 and ankyrin 1 ion channel activation on sensory neurons via lipid raft modification},
	url = {https://m2.mtmt.hu/api/publication/31387590},
	author = {Payrits, Maja and Horváth, Ádám and Biró-Sütő, Tünde and Erostyák, János and Makkai, Géza and Sághy, Éva and Pohóczky, Krisztina and Kecskés, Angéla and Kecskés, Miklós and Szolcsányi, János and Helyes, Zsuzsanna and Szőke, Éva},
	doi = {10.3390/ijms21145019},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {21},
	unique-id = {31387590},
	issn = {1661-6596},
	abstract = {Transient Receptor Potential Vanilloid 1 and Ankyrin 1 (TRPV1, TRPA1) cation channels are expressed in nociceptive primary sensory neurons and regulate nociceptor and inflammatory functions. Resolvins are endogenous lipid mediators. Resolvin D1 (RvD1) is described as a selective inhibitor of TRPA1-related postoperative and inflammatory pain in mice acting on the G protein-coupled receptor DRV1/GPR32. Resolvin D2 (RvD2) is a very potent TRPV1 and TRPA1 inhibitor in DRG neurons, and decreases inflammatory pain in mice acting on the GPR18 receptor, via TRPV1/TRPA1-independent mechanisms. We provided evidence that resolvins inhibited neuropeptide release from the stimulated sensory nerve terminals by TRPV1 and TRPA1 activators capsaicin (CAPS) and allyl-isothiocyanate (AITC), respectively. We showed that RvD1 and RvD2 in nanomolar concentrations significantly decreased TRPV1 and TRPA1 activation on sensory neurons by fluorescent calcium imaging and inhibited the CAPS-and AITC-evoked45Ca-uptake on TRPV1-and TRPA1-expressing CHO cells. Since CHO cells are unlikely to express resolvin receptors, resolvins are suggested to inhibit channel opening through surrounding lipid raft disruption. Here, we proved the ability of resolvins to alter the membrane polarity related to cholesterol composition by fluorescence spectroscopy. It is concluded that targeting lipid raft integrity can open novel peripheral analgesic opportunities by decreasing the activation of nociceptors. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.},
	keywords = {LIPID RAFTS; transient receptor potential channel; sensory neuron; resolvin D1; nerve terminal; Resolvin D2},
	year = {2020},
	eissn = {1422-0067},
	orcid-numbers = {Sághy, Éva/0000-0002-4031-3461; Pohóczky, Krisztina/0000-0003-0385-5162}
}

@misc{MTMT:33575473,
	title = {Luminescence spectroscopy study of silicon carbide nanoparticles of 1-10 nm Complex analysis of spectroscopy measurements data},
	url = {https://m2.mtmt.hu/api/publication/33575473},
	author = {Erostyák, János},
	unique-id = {33575473},
	year = {2019}
}

@misc{MTMT:33575151,
	title = {Transition from solid state to molecular-like optical properties in siliconcarbide nanoparticles},
	url = {https://m2.mtmt.hu/api/publication/33575151},
	author = {Erostyák, János},
	unique-id = {33575151},
	year = {2019}
}