TY - JOUR AU - Bókony, Veronika AU - Balogh, Emese AU - Ujszegi, János AU - Ujhegyi, Nikolett AU - Szederkényi, Márk AU - Hettyey, Attila TI - Tadpoles Develop Elevated Heat Tolerance in Urban Heat Islands Regardless of Sex JF - EVOLUTIONARY BIOLOGY J2 - EVOL BIOL PY - 2024 SN - 0071-3260 DO - 10.1007/s11692-024-09626-7 UR - https://m2.mtmt.hu/api/publication/34561992 ID - 34561992 N1 - Department of Evolutionary Ecology, HUN-REN Centre for Agricultural Research, Plant Protection Institute, Herman Ottó út 15, Budapest, 1022, Hungary Molecular Ecology Research Group, Department of Zoology, University of Veterinary Medicine Budapest, István u. 2, Budapest, 1078, Hungary Department of Systematic Zoology and Ecology, Institute of Biology, Eötvös Loránd University, Pázmány Péter stny. 1/C, Budapest, 1117, Hungary Export Date: 20 February 2024 Correspondence Address: Bókony, V.; Department of Evolutionary Ecology, Herman Ottó út 15, Hungary; email: bokony.veronika@atk.hun-ren.hu AB - The ability of wildlife to endure the effects of high temperatures is increasingly important for biodiversity conservation under climate change and spreading urbanization. Organisms living in urban heat islands can have elevated heat tolerance via phenotypic or transgenerational plasticity or microevolution. However, the prevalence and mechanisms of such thermal adaptations are barely known in aquatic organisms. Furthermore, males and females can differ in heat tolerance, which may lead to sex-biased mortality, yet it is unknown how sex differences in thermal biology influence urban phenotypic divergence. To address these knowledge gaps, we measured critical thermal maxima (CTmax) in male and female agile frog (Rana dalmatina) tadpoles captured from warm urban ponds and cool woodland ponds, and in a common-garden experiment where embryos collected from both habitat types were raised in the laboratory. We found higher CTmax in urban-dwelling tadpoles compared to their counterparts living in woodland ponds. This difference was reversed in the common-garden experiment: tadpoles originating from urban ponds had lower CTmax than tadpoles originating from woodland ponds. We found no effect of sex on CTmax or its difference between habitats. These results demonstrate that aquatic amphibian larvae can respond to the urban heat island effect with increased heat tolerance similarly to other, mostly terrestrial taxa studied so far, and that phenotypic plasticity may be the main driver of this response. Our findings also suggest that heat-induced mortality may be independent of sex in tadpoles, but research is needed in many more taxa to explore potentially sex-dependent urban thermal responses. LA - English DB - MTMT ER - TY - JOUR AU - Hettyey, Attila AU - Páll-Gergely, Barna TI - Editorial JF - ACTA ZOOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE J2 - ACTA ZOOLOG ACAD SCI HUNG VL - 69 PY - 2023 IS - 3 SP - 197 EP - 198 PG - 2 SN - 1217-8837 DO - 10.17109/AZH.69.3.197.2023 UR - https://m2.mtmt.hu/api/publication/34740499 ID - 34740499 LA - English DB - MTMT ER - TY - CHAP AU - Mikó, Zsanett AU - Bókony, Veronika AU - Ujszegi, János AU - Hettyey, Attila ED - Kontschán, Jenő TI - Egy glifozátalapú gyomirtó ökotoxikológiai hatásai a vízi szervezetekre T2 - Tudományos kutatások a környezetbarát növényvédelemért a HUN-REN ATK Növényvédelmi Intézetében PB - Inform Kiadó és Nyomda Kft. CY - Budapest SN - 9786155666636 PY - 2023 SP - 8 EP - 11 PG - 4 UR - https://m2.mtmt.hu/api/publication/34547623 ID - 34547623 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hettyey, Attila AU - Kásler, Andrea AU - Ujszegi, János AU - Herczeg, Dávid TI - A felülfertőződés ökológiája kétéltűeknél: Különböző paraziták, ha egyszerre támadnak JF - ÉLET ÉS TUDOMÁNY J2 - ÉLET ÉS TUDOMÁNY VL - 47 PY - 2023 SP - 1484 EP - 1487 PG - 4 SN - 0013-6077 UR - https://m2.mtmt.hu/api/publication/34506237 ID - 34506237 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Ujszegi, János AU - Boros, Zsófia AU - Fodor, András AU - Vajna, Balázs AU - Hettyey, Attila TI - Metabolites of Xenorhabdus bacteria are potent candidates for mitigating amphibian chytridiomycosis JF - AMB EXPRESS J2 - AMB EXPRESS VL - 13 PY - 2023 IS - 1 SN - 2191-0855 DO - 10.1186/s13568-023-01585-0 UR - https://m2.mtmt.hu/api/publication/34111344 ID - 34111344 N1 - Department of Evolutionary Ecology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Budapest, Hungary Department of Systematic Zoology and Ecology, Eötvös Loránd University, Budapest, Hungary Department of Genetics, Eötvös Loránd University, Budapest, Hungary Department of Microbiology, Eötvös Loránd University, Budapest, Hungary Export Date: 8 September 2023 Correspondence Address: Ujszegi, J.; Department of Systematic Zoology and Ecology, Hungary; email: ujszegi.janos@gmail.com AB - Chytridiomycosis, caused by the chytrid fungus Batrachochytrium dendrobatidis (Bd), has caused extreme losses in amphibian biodiversity. Finding bacteria that produce metabolites with antifungal properties may turn out to be invaluable in the fight against this devastating disease. The entomopathogenic bacteria, Xenorhabdus szentirmaii and X. budapestensis produce secondary metabolites that are effective against a wide range of fungal plant pathogens. To assess whether they may also be effective against Bd, we extracted cell-free culture media (CFCM) from liquid cultures of X. szentirmaii and X. budapestensis and tested their ability to inhibit Bd growth in vitro. As a second step, using juvenile common toads ( Bufo bufo ) experimentally infected with Bd we also tested the in vivo antifungal efficacy of X. szentirmaii CFCM diluted to 2 and 10% (v/v), while also assessing possible malign side effects on amphibians. Results of the in vitro experiment documented highly effective growth inhibition by CFCMs of both Xenorhabdus species. The in vivo experiment showed that treatment with CFCM of X. szentirmaii applied at a dilution of 10% resulted in infection intensities reduced by ca. 73% compared to controls and to juvenile toads treated with CFCM applied at a dilution of 2%. At the same time, we detected no negative side effects of treatment with CFCM on toad survival and development. Our results clearly support the idea that metabolites of X. szentirmaii , and perhaps of several other Xenorhabdus species as well, may prove highly useful for the treatment of Bd infected amphibians. LA - English DB - MTMT ER - TY - JOUR AU - Herczeg, Dávid AU - Holly, Dóra AU - Kásler, Andrea AU - Bókony, Veronika AU - Papp, Tibor AU - Takács-Vágó, Hunor AU - Ujszegi, János AU - Hettyey, Attila TI - Amphibian larvae benefit from a warm environment under simultaneous threat from chytridiomycosis and ranavirosis JF - OIKOS J2 - OIKOS VL - 11 PY - 2023 PG - 11 SN - 0030-1299 DO - 10.1111/oik.09953 UR - https://m2.mtmt.hu/api/publication/34074949 ID - 34074949 N1 - Department of Evolutionary Ecology, Centre for Agricultural Research, Plant Protection Institute, Eötvös Loránd Research Network, Budapest, Hungary ELKH-ELTE-MTM Integrative Ecology Research Group, Budapest, Hungary Department of Systematic Zoology and Ecology, Eötvös Loránd University, Budapest, Hungary Doctoral School of Biology, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Hungary Institute for Veterinary Medical Research, Eötvös Loránd Research Network, Budapest, Hungary Department of Ecology, University of Veterinary Medicine, Budapest, Hungary Export Date: 29 August 2023 CODEN: OIKSA Correspondence Address: Herczeg, D.; Department of Evolutionary Ecology, Hungary; email: herczegdavid88@gmail.com LA - English DB - MTMT ER - TY - JOUR AU - Üveges, Bálint AU - Kalina, C AU - Szabó, Krisztina AU - Móricz, Ágnes AU - Holly, Dóra AU - Gabor, C R AU - Hettyey, Attila AU - Bókony, Veronika TI - Does The Glucocorticoid Stress Response Make Toads More Toxic? An Experimental Study on The Regulation of Bufadienolide Toxin Synthesis JF - Integrative Organismal Biology VL - 5 PY - 2023 IS - 1 SP - 1 EP - 13 PG - 13 SN - 2517-4843 DO - 10.1093/iob/obad021 UR - https://m2.mtmt.hu/api/publication/34010493 ID - 34010493 N1 - Department of Evolutionary Ecology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Herman Ottó út 15, Budapest, 1022, Hungary Molecular Ecology and Evolution at Bangor, School of Natural Sciences, Bangor University, Environment Centre Wales, Bangor, LL57 2UW, United Kingdom Department of Ecology, Institute of Biology, University of Veterinary Medicine, István u. 2, Budapest, 1078, Hungary Division of Clinical Immunology, Department for Internal Medicine, Faculty of Medicine, University of Debrecen, Móricz Zsigmond út 22, Debrecen, 4032, Hungary Department of Pathophysiology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Herman Ottóút 15, Budapest, 1022, Hungary Department of Biology, College of Science and Engineering, Texas State University, 601 University Dr., San Marcos, TX 78666, United States Export Date: 29 August 2023 Correspondence Address: Üveges, B.; Department of Evolutionary Ecology, Herman Ottó út 15, Hungary; email: uveges.balint@yahoo.de AB - Chemical defence is a crucial component of fitness in many organisms, yet the physiological regulation of defensive toxin synthesis is poorly understood, especially in vertebrates. Bufadienolides, the main defensive compounds of toads, are toxic to many predators and other natural enemies, and their synthesis can be upregulated by stressors including predation risk, high conspecific density, and pollutants. Thus, higher toxin content may be the consequence of a general endocrine stress response in toads. Therefore, we hypothesized that bufadienolide synthesis may be stimulated by elevated levels of corticosterone (CORT), the main glucocorticoid hormone of amphibians, or by upstream regulators that stimulate CORT production. To test these alternatives, we treated common toad tadpoles with exogenous CORT (exoCORT) or metyrapone (MTP, a CORT-synthesis inhibitor that stimulates upstream regulators of CORT by negative feedback) in the presence or absence of predation cues for 2 or 6 days, and subsequently measured their CORT release rates and bufadienolide content. We found that CORT release rates were elevated by exoCORT, and to a lesser extent also by MTP, regardless of treatment length. Bufadienolide content was significantly decreased by treatment with exoCORT for 6 days, but was unaffected by exposure to exoCORT for 2 days or to MTP for either 6 or 2 days. Presence or absence of predation cues affected neither CORT release rate nor bufadienolide content. Our results suggest that changes in bufadienolide synthesis in response to environmental challenges are not driven by CORT but may rather be regulated by upstream hormones of the stress response. LA - English DB - MTMT ER - TY - CONF AU - Kásler, Andrea AU - Bókony, Veronika AU - Mikó, Zsanett AU - Herczeg, Dávid AU - Ujszegi, János AU - Holly, Dóra AU - Hettyey, Attila ED - HALTRICH, ATTILA TI - A klímaváltozás és két piretroid rovarölőszer együttes hatásai juvenilis barna varangyok (Bufo bufo) fejlődésére és túlélésére T2 - 69. NÖVÉNYVÉDELMI TUDOMÁNYOS NAPOK PB - Magyar Növényvédelmi Társaság C1 - Budapest T3 - Növényvédelmi Tudományos Napok, ISSN 0231-2956 ; 69. PY - 2023 SP - 36 UR - https://m2.mtmt.hu/api/publication/33661348 ID - 33661348 LA - Hungarian DB - MTMT ER - TY - CONF AU - Hettyey, Attila AU - Kásler, Andrea AU - Ujszegi, János AU - Holly, Dóra AU - Herczeg, Dávid ED - HALTRICH, ATTILA TI - Ökotoxikológia egy komplex világban: peszticidek és patogének együttes hatásai erdei békákra (Rana dalmatina) a korai életszakaszban T2 - 69. NÖVÉNYVÉDELMI TUDOMÁNYOS NAPOK PB - Magyar Növényvédelmi Társaság C1 - Budapest T3 - Növényvédelmi Tudományos Napok, ISSN 0231-2956 ; 69. PY - 2023 SP - 34 UR - https://m2.mtmt.hu/api/publication/33661330 ID - 33661330 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Mikó, Zsanett AU - Hettyey, Attila TI - Toxicity of POEA-containing glyphosate-based herbicides to amphibians is mainly due to the surfactant, not to the active ingredient JF - ECOTOXICOLOGY J2 - ECOTOXICOLOGY VL - 32 PY - 2023 IS - 2 SP - 150 EP - 159 PG - 10 SN - 0963-9292 DO - 10.1007/s10646-023-02626-x UR - https://m2.mtmt.hu/api/publication/33657273 ID - 33657273 N1 - Export Date: 8 May 2023 CODEN: ECOTE Correspondence Address: Mikó, Z.; Department of Evolutionary Ecology, Herman Ottó út 15, Hungary; email: miko.zsanett@atk.hu AB - Current international legislation regarding agrochemicals requires thorough toxicological testing mainly of the active ingredients. In a 96-h acute toxicity test we exposed Rana dalmatina and Bufo bufo tadpoles to either one of three concentrations of glyphosate, three concentrations of the surfactant (POEA), three concentrations of the two components together, or to non-contaminated water (control), and subsequently assessed mortality and body mass. To investigate whether simultaneous exposure to another stress factor influences effects of the contaminants, we performed tests both in the presence or absence of predator chemical cues. We found that the surfactant had significant harmful effects on tadpoles; survival was lowered by the highest concentration of the surfactant in case of R. dalmatina , while in B. bufo tadpoles it reduced survival already at medium concentrations. Body mass was significantly influenced by medium and high surfactant concentrations in both species. The presence of glyphosate did not have a significant effect by itself, but it slightly increased mortality in tadpoles exposed to medium concentrations of the surfactant in both species. The presence of chemical cues did not have an effect on the examined variables. Our study confirms that the toxicity of glyphosate-based herbicides is mainly due to the examined surfactant. Nonetheless, we found that glyphosate can enhance the harmful effect of the surfactant. These results stress that during the authorization process of new pesticide formulations, not only the active ingredients would need to be examined but the excipients should also be taken into account in an obligatory and systematic manner. LA - English DB - MTMT ER -