TY - JOUR AU - Oprisan, Sorinel A AU - Clementsmith, Xandre AU - Tompa, Tamás AU - Lavin, Antonieta TI - Empirical mode decomposition of local field potential data from optogenetic experiments. JF - FRONTIERS IN COMPUTATIONAL NEUROSCIENCE J2 - FRONT COMPUT NEUROSC VL - 17 PY - 2023 PG - 16 SN - 1662-5188 DO - 10.3389/fncom.2023.1223879 UR - https://m2.mtmt.hu/api/publication/34082472 ID - 34082472 AB - This study investigated the effects of cocaine administration and parvalbumin-type interneuron stimulation on local field potentials (LFPs) recorded in vivo from the medial prefrontal cortex (mPFC) of six mice using optogenetic tools.The local network was subject to a brief 10 ms laser pulse, and the response was recorded for 2 s over 100 trials for each of the six subjects who showed stable coupling between the mPFC and the optrode. Due to the strong non-stationary and nonlinearity of the LFP, we used the adaptive, data-driven, Empirical Mode Decomposition (EMD) method to decompose the signal into orthogonal Intrinsic Mode Functions (IMFs).Through trial and error, we found that seven is the optimum number of orthogonal IMFs that overlaps with known frequency bands of brain activity. We found that the Index of Orthogonality (IO) of IMF amplitudes was close to zero. The Index of Energy Conservation (IEC) for each decomposition was close to unity, as expected for orthogonal decompositions. We found that the power density distribution vs. frequency follows a power law with an average scaling exponent of ~1.4 over the entire range of IMF frequencies 2-2,000 Hz.The scaling exponent is slightly smaller for cocaine than the control, suggesting that neural activity avalanches under cocaine have longer life spans and sizes. LA - English DB - MTMT ER - TY - CONF AU - Nagy, Dominika AU - Plauska, Andrius AU - Szalay, Gergely AU - Marosi, Máté Gábor AU - Pinke, Domonkos Péter AU - Csupernyák, Csaba AU - Bojdán, Alexandra AU - Szepesi, Áron AU - Tompa, Tamás AU - Ócsai, Katalin AU - Katona, Gergely AU - Rózsa J., Balázs TI - Stable, multi-day, functional measurement to study cellular level plasticity in mouse primary visual cortex by fast 3D imaging T2 - IBRO Workshop PY - 2020 UR - https://m2.mtmt.hu/api/publication/31348265 ID - 31348265 N1 - Poster AB - We investigated how 3D representation of the visual information, which is perceived and understood by the behaving animals in the primary visual cortex (V1), is changed while the animal engaged in a visual task. To reach this goal we use in vivo two-photon acusto-optic microscopy (with AAV-Syn-GCaMP6s or AAV-Syn-jRGECO1a) to record cellular responses (through different cortical layers) to visual stimuli in V1 before (baseline) and after (effect) visual training. During training period, the mice learn to discriminate visual landmarks in a virtual reality.To study cellular plasticity in time we had to measure the same neuronal ensembles (up to 200 cells) during the baseline and effect period which can be apart in time (10-20 days). As neuronal responses are very sensitive to the spatial inconsistency of recording coordinates, therefore, orientation tuning and other properties of the recorded visually evoked responses could be contaminated with recording artifacts. To resolve this critical issue, we used 3D AO drift scanning microscopy, which can extend each scanning point to small 3D line-, surface- or volume-elements. Furthermore, we are able to scan small volumes (40x40x40 µm) around each cell bodies in 3D, and use these mini-volume information to carefully and precisely realign all individual scanning regions at each measurement day, significantly reducing the chance of mismatching the recording coordinates. AO scanning of hundreds of cells at cortical depths up to 1 mm makes it feasible to examine the effect of learning in behavior experiments both at single-cell and at network scales. We found that, in contrast to previous theories, adult mice brain is plastic as the temporal dynamics of the visual representation can change at multiple temporal scales following visual learning in individual neurons. LA - English DB - MTMT ER - TY - CHAP AU - Szalay, Gergely AU - Szadai, Zoltán AU - Judák, Linda AU - Maák, Pál AU - Ócsai, K. AU - Veress, Máté AU - Tompa, Tamás AU - Chiovini, Balázs AU - Katona, G. AU - Rózsa J., Balázs TI - Fast 3D imaging and photostimulation by 3D acousto-optical microscopy revealed spatiotemporally orchestrated clusters in the visual cortex T2 - Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP) PB - Optical Society of America (OSA) CY - Washington DC SN - 9781943580545 PY - 2019 DO - 10.1364/BRAIN.2019.BM3A.1 UR - https://m2.mtmt.hu/api/publication/31920226 ID - 31920226 N1 - Laboratory of 3D Functional Network and Dendritic Imaging, Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony street 43, Budapest, 1083, Hungary Pázmány Péter Catholic University, Szentkirályi street 28, Budapest, 1088, Hungary Department of Atomic Physics, Budapest University of Technology and Economics, Budafoki street 8, Budapest, H-1111, Hungary Conference code: 158803 Export Date: 15 February 2024 Correspondence Address: Szalay, G.; Laboratory of 3D Functional Network and Dendritic Imaging, Szigony street 43, Hungary; email: rozsabal@koki.hu AB - Simultaneously with fast 3D imaging, we generated holograms from multiple spots by introducing sine waves with non-linear frequency chirps into the acousto-optical deflectors and activated over 40cells with high resolution within 16ms in large volumes. © 2019 The Authors. LA - English DB - MTMT ER - TY - JOUR AU - Oprisan, Sorinel A AU - Clementsmith, Xandre AU - Tompa, Tamás AU - Lavin, Antonieta TI - Dopamine receptor antagonists effects on low-dimensional attractors of local field potentials in optogenetic mice. JF - PLOS ONE J2 - PLOS ONE VL - 14 PY - 2019 IS - 10 SP - e0223469 SN - 1932-6203 DO - 10.1371/journal.pone.0223469 UR - https://m2.mtmt.hu/api/publication/30923743 ID - 30923743 AB - The goal of this study was to investigate the effects of acute cocaine injection or dopamine (DA) receptor antagonists on the medial prefrontal cortex (mPFC) gamma oscillations and their relationship to short term neuroadaptation that may mediate addiction. For this purpose, optogenetically evoked local field potentials (LFPs) in response to a brief 10 ms laser light pulse were recorded from 17 mice. D1-like receptor antagonist SCH 23390 or D2-like receptor antagonist sulpiride, or both, were administered either before or after cocaine. A Euclidian distance-based dendrogram classifier separated the 100 trials for each animal in disjoint clusters. When baseline and DA receptor antagonists trials were combined in a single trial, a minimum of 20% overlap occurred in some dendrogram clusters, which suggests a possible common, invariant, dynamic mechanism shared by both baseline and DA receptor antagonists data. The delay-embedding method of neural activity reconstruction was performed using the correlation time and mutual information to determine the lag/correlation time of LFPs and false nearest neighbors to determine the embedding dimension. We found that DA receptor antagonists applied before cocaine cancels out the effect of cocaine and leaves the lag time distributions at baseline values. On the other hand, cocaine applied after DA receptor antagonists shifts the lag time distributions to longer durations, i.e. increase the correlation time of LFPs. Fourier analysis showed that a reasonable accurate decomposition of the LFP data can be obtained with a relatively small (less than ten) Fourier coefficients. LA - English DB - MTMT ER - TY - CONF AU - Nagy, D. AU - Szalay, Gergely AU - Marosi, Máté Gábor AU - Plauska, Andrius AU - Pinke, D. AU - Csupernyák, Cs. AU - Heizer, B. AU - Bojdán, Alexandra AU - Horváth, G. AU - Tompa, Tamás AU - Judák, Linda AU - Maák, Pál AU - Veress, Máté AU - Fehér, András AU - Ócsai, K. AU - Katona, Gergely AU - Rózsa J., Balázs TI - Longterm Calcium Imaging With 3D-Acusto-Optic Microscopy at more than 1 mm Cortical Depth During Learning T2 - 16th Annual Conference of the Hungarian Neuroscience Society, Hungarian Academy of Sciences, Debrecen PY - 2019 SP - 92 UR - https://m2.mtmt.hu/api/publication/30659378 ID - 30659378 LA - English DB - MTMT ER - TY - CONF AU - Ócsai, K. AU - Plauska, Andrius AU - Szalay, Gergely AU - Marosi, Máté Gábor AU - Tompa, Tamás AU - Judák, Linda AU - Pinke, D. AU - Nagy, D. AU - Csupernyák, Cs. AU - Heizer, B. AU - Horváth, G. AU - Maák, Pál AU - Veress, Máté AU - Fehér, András AU - Rózsa J., Balázs AU - Katona, Gergely TI - Signal shape based functional neuron clusters from two photon imaging of learning mice T2 - 16th Annual Conference of the Hungarian Neuroscience Society, Hungarian Academy of Sciences, Debrecen PY - 2019 SP - 89 UR - https://m2.mtmt.hu/api/publication/30659344 ID - 30659344 LA - English DB - MTMT ER - TY - CONF AU - Szalay, Gergely AU - Marosi, Máté Gábor AU - D, Pinke AU - Plauska, Andrius AU - Cs, Csupernyák AU - D, Nagy AU - B, Heizer AU - G, Horváth AU - Tompa, Tamás AU - Katona, Gergely AU - Rózsa J., Balázs TI - Multi-day, stable, functional neuronal network measurement in three dimension from a large volume at more than 1 mm cortical depth T2 - 11th Forum of European Neuroscience PY - 2018 SP - n/a UR - https://m2.mtmt.hu/api/publication/30659329 ID - 30659329 LA - English DB - MTMT ER - TY - CONF AU - Plauska, Andrius AU - Szalay, Gergely AU - Marosi, Máté Gábor AU - Tompa, Tamás AU - D., Pinke AU - G., Horváth AU - D., Nagy AU - C., Csupernyák AU - B., Heizler AU - Judák, Linda AU - Maák, Pál AU - Veress, Máté AU - Fehér, András AU - Katona, Gergely AU - Rózsa J., Balázs TI - Multi-day, stable, functional neuronal network measurement in three dimensions from a depth exceeding 1mm T2 - SFN 2018 - the annual meeting of the SOCIETY OF NEUROSCIENCE PY - 2018 UR - https://m2.mtmt.hu/api/publication/30659322 ID - 30659322 LA - English DB - MTMT ER - TY - JOUR AU - Oprisan, Sorinel A AU - Imperatore, Julia AU - Helms, Jessica AU - Tompa, Tamás AU - Lavin, Antonieta TI - Cocaine-Induced Changes in Low-Dimensional Attractors of Local Field Potentials in Optogenetic Mice JF - FRONTIERS IN COMPUTATIONAL NEUROSCIENCE J2 - FRONT COMPUT NEUROSC VL - 12 PY - 2018 PG - 16 SN - 1662-5188 DO - 10.3389/fncom.2018.0002 UR - https://m2.mtmt.hu/api/publication/27317346 ID - 27317346 LA - English DB - MTMT ER - TY - CHAP AU - Szalay, Gergely AU - Szadai, Zoltán AU - Judák, Linda AU - Pál, Maák AU - Katalin, Ócsai AU - Máté, Veress AU - Tompa, Tamás AU - Chiovini, Balázs AU - Katona, Gergely AU - Rózsa J., Balázs TI - Fast 3D imaging and re-activation of neuronal networks, dendrites, and spines in several cubic millimeter volumes during visual learning to understand visual representation T2 - Clinical and Translational Biophotonics, TRANSLATIONAL 2018 PB - Optical Society of America (OSA) CY - Washington DC SN - 9781943580415 T3 - Optics InfoBase Conference Papers ; Part F91-TRANSLATIONAL 2018. PY - 2018 DO - 10.1364/BRAIN.2018.BTu2C.1 UR - https://m2.mtmt.hu/api/publication/3398282 ID - 3398282 AB - Our long-term aim is to assess the feasibility of creating an “artificial sense” and, thereby, a possible sensory (visual) prosthetic. While working towards this goal, we are investigating how neural assembly activity relates to subjective perceptions. Recording, finding and understanding these functional assemblies will make it possible to reactivate them in a precise, biologically relevant manner to elicit cortical activation similar to visual stimulation. LA - English DB - MTMT ER -