TY - JOUR AU - Hrabák, András AU - Bőgel, Gábor AU - Murányi, József AU - Tamási, Viola AU - Németh, Krisztina AU - Szokol, Bálint AU - Kukor, Zoltán AU - Kardon Tamás, Zoltán AU - Őrfi, László TI - Decreasing effects of protein kinase inhibitors on the expression of NOS2 and inflammatory cytokines and on phagocytosis in rat peritoneal macrophages is partly related to repolarization JF - MOLECULAR IMMUNOLOGY J2 - MOL IMMUNOL VL - 153 PY - 2023 SP - 10 EP - 24 PG - 15 SN - 0161-5890 DO - 10.1016/j.molimm.2022.11.002 UR - https://m2.mtmt.hu/api/publication/33254004 ID - 33254004 LA - English DB - MTMT ER - TY - JOUR AU - Csomó, Krisztián Benedek AU - Belik, Andrea AU - Hrabák, András AU - Kovács, Bence Géza AU - Fábián, Orsolya AU - Valent, Sándor AU - Varga, Gábor AU - Kukor, Zoltán TI - Effect of Pravastatin and Simvastatin on the Reduction of Cytochrome C. JF - JOURNAL OF PERSONALIZED MEDICINE J2 - J PERS MED VL - 12 PY - 2022 IS - 7 PG - 10 SN - 2075-4426 DO - 10.3390/jpm12071121 UR - https://m2.mtmt.hu/api/publication/33034057 ID - 33034057 AB - Statins are used to treat hypercholesterolemia, with several pleiotropic effects. Alongside their positive effects (for example, decreasing blood pressure), they can also bring about negative effects/symptoms (such as myopathy). Their main mechanism of action is inducing apoptosis, the key step being the release of cytochrome c from the mitochondria. This can be facilitated by oxidative stress, through which glutathione is oxidized. In this research, glutathione was used as a respiratory substrate to measure the mitochondrial oxygen consumption of rat liver with an O2 electrode. The reduction of cytochrome c was monitored photometrically. Hydrophilic (pravastatin) and lipophilic (simvastatin) statins were used for the measurements. Pravastatin reduces the reduction of cytochrome c and the oxygen consumption of the mitochondria, while simvastatin, on the other hand, increases the reduction of cytochrome c and the mitochondrial oxygen consumption. The results make it seem probable that statins influence the mitochondrial oxygen consumption through cytochrome c. Simvastatin could enhance the oxidizing capacity of free cytochrome c, thereby increasing oxidative stress and thus facilitating apoptosis. The observed effects could further the understanding of the mechanism of action of statins and thereby aid in constructing optimal statin therapy for every patient. LA - English DB - MTMT ER - TY - JOUR AU - Bőgel, Gábor AU - Murányi, József AU - Szokol, Bálint AU - Kukor, Zoltán AU - Móra , István András AU - Kardon Tamás, Zoltán AU - Őrfi, László AU - Hrabák, András TI - Production of NOS2 and inflammatory cytokines is reduced by selected protein kinase inhibitors with partial repolarization of HL-60 derived and human blood macrophages JF - HELIYON J2 - HELIYON VL - 8 PY - 2022 IS - 1 PG - 16 SN - 2405-8440 DO - 10.1016/j.heliyon.2021.e08670 UR - https://m2.mtmt.hu/api/publication/32574856 ID - 32574856 LA - English DB - MTMT ER - TY - JOUR AU - Csomó, Krisztián Benedek AU - Alasztics, Bálint AU - Sándor, A. P. AU - Belik, Andrea AU - Varga, Gábor AU - Hrabák, András AU - Kukor, Zoltán TI - Characterization of oxidation of glutathione by cytochrome c JF - JOURNAL OF BIOENERGETICS AND BIOMEMBRANES J2 - J BIOENERG BIOMEMBR VL - 54 PY - 2022 IS - 1 SP - 1 EP - 8 PG - 8 SN - 0145-479X DO - 10.1007/s10863-021-09926-z UR - https://m2.mtmt.hu/api/publication/32534532 ID - 32534532 LA - English DB - MTMT ER - TY - JOUR AU - Pánczél, Zita AU - Kukor, Zoltán AU - Supák, Dorina AU - Kovács, Bence Géza AU - Kecskeméti, András AU - Czizel, Rita AU - Djurecz, Magdolna AU - Alasztics, Bálint AU - Csomó, Krisztián Benedek AU - Hrabák, András AU - Valent, Sándor TI - Pravastatin induces NO synthesis by enhancing microsomal arginine uptake in healthy and preeclamptic placentas JF - BMC PREGNANCY AND CHILDBIRTH J2 - BMC PREGNANCY CHILDB VL - 19 PY - 2019 IS - 1 PG - 9 SN - 1471-2393 DO - 10.1186/s12884-019-2507-0 UR - https://m2.mtmt.hu/api/publication/30929732 ID - 30929732 LA - English DB - MTMT ER - TY - CHAP AU - Hrabák, András ED - Bánhegyi, Gábor ED - Sipeki, Szabolcs TI - Lipidek anyagcseréje T2 - Biokémia, molekuláris és sejtbiológia PB - Semmelweis Kiadó CY - Budapest SN - 9789633314258 PY - 2018 SP - 304 EP - 338 PG - 35 UR - https://m2.mtmt.hu/api/publication/30556140 ID - 30556140 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Hrabák, András AU - Kukor, Zoltán TI - Is Arginine Truly a “Miracle Molecule” ?: A Critical Evaluation of Nutritional Arginine Supplementation from a Biochemical Point of View T2 - L-Arginine: Structure, Dietary Sources and Beneficial Effects PB - Nova Science Publishers CY - New York, New York SN - 9781634853293 T3 - Biochemistry Research Trends PY - 2016 SP - 1 EP - 46 PG - 46 UR - https://m2.mtmt.hu/api/publication/3105245 ID - 3105245 LA - English DB - MTMT ER - TY - JOUR AU - Marczell, István AU - Hrabák, András AU - Nyírő, Gábor AU - Patócs, Attila Balázs AU - Stark, Júlia AU - Dinya, Elek AU - Kukor, Zoltán AU - Tóth, Sára AU - Tulassay, Zsolt AU - Rácz, Károly AU - Békési, Gábor TI - 17-beta-estradiol Decreases Neutrophil Superoxide Production through Rac1 JF - EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES J2 - EXP CLIN ENDOCR DIAB VL - 124 PY - 2016 IS - 10 SP - 588 EP - 592 PG - 5 SN - 0947-7349 DO - 10.1055/s-0042-105556 UR - https://m2.mtmt.hu/api/publication/3102371 ID - 3102371 N1 - 2nd Department of Medicine, Semmelweis University, Szentkirályi utca 46, Budapest, H-1088, Hungary Department of Medical Chemistry Molecular Biology and Pathobiochemistry, Semmelweis University, TÅzoltó u. 37-47, Budapest, H-1094, Hungary Office for Research Groups Attached to Universities and Other Institutions of the Hungarian, Academy of Sciences, Molecular Medicine Research Group, Szentkirályi str. 46, Budapest, H-1088, Hungary Hungarian Academy of Sciences, Lendulet, 2013, Hungary Faculty of Health and Public Services, Institute of Health Informatics Development and Further Training, Semmelweis University, Ulloi ut 78/b, Budapest, Hungary Department of Genetics Cell- and Immunobiology, Nagyvárad tér 4, Budapest, H-1089, Hungary Cited By :4 Export Date: 17 September 2019 CODEN: ECEDF Correspondence Address: Marczell, I.; 2nd Department of Medicine, Semmelweis University, Szentkirályi utca 46, Hungary; email: istvan.marczell@gmail.hu Chemicals/CAS: estradiol, 50-28-2; reduced nicotinamide adenine dinucleotide phosphate oxidase, 9032-22-8; superoxide, 11062-77-4; Estradiol; rac1 GTP-Binding Protein; RAC1 protein, human; Superoxides AB - Neutrophil granulocytes form the biggest free radical producing system of the human body. The importance of this system in atherosclerotic plaque formation and other free radical mediated disorders is confirmed by both in vivo and in vitro studies. Estrogen's effect on free radical production involves multiple estrogen receptors and occurs both on transcriptional and on protein phosphorylational level. Estrogen decreases the superoxide production of neutrophil granulocytes in such a short time frame it is unlikely to be mediated by transcription regulation. We investigated the underlying mechanism through which the mentioned estrogen effect takes place using an immunabsorption-based method. Phosphorylation data of 43 different messenger proteins were used for pathway analysis. The newly identified pathway involved largely second messengers from previously described non-genomic estrogen effects and affected superoxide production via Rac1 - an important regulator of free radical production and chemotaxis. Selective inhibition of the participating second messengers altered superoxide production in the predicted direction confirming that this pathway is at least partly responsible for the effect of 17-beta-estradiol on chemoattractant induced superoxide production. LA - English DB - MTMT ER - TY - CHAP AU - Hrabák, András ED - Bánhegyi, Gábor ED - Sipeki, Szabolcs TI - Lipidek anyagcseréje T2 - Biokémia, molekuláris és sejtbiológia PB - Semmelweis Kiadó és Multimédia Stúdió CY - Budapest SN - 9789633310137 PY - 2015 SP - 262 EP - 297 PG - 36 UR - https://m2.mtmt.hu/api/publication/2892042 ID - 2892042 LA - Hungarian DB - MTMT ER - TY - BOOK AU - Hrabák, András TI - Molekuláris biológiai módszerek 2. bővített kiadás. Egészségügyi mérnökhallgatók számára TS - Egészségügyi mérnökhallgatók számára ET - 0 PB - Semmelweis Kiadó és Multimédia Stúdió CY - Budapest PY - 2014 SN - 9789633311578 UR - https://m2.mtmt.hu/api/publication/2820555 ID - 2820555 LA - Hungarian DB - MTMT ER -