TY - JOUR AU - Dombrádi, Viktor Béla TI - 100 éves a Debreceni Egyetem Orvosi Vegytani Intézete JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - XLV PY - 2021 IS - 4 SP - 33 EP - 43 PG - 11 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32558272 ID - 32558272 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hajdu, Tímea AU - Szabó, Krisztina AU - Jakab, Ágnes AU - Pócsi, István AU - Dombrádi, Viktor Béla AU - Nagy, Péter TI - Biophysical experiments reveal a protective role of protein phosphatase Z1 against oxidative damage of the cell membrane in Candida albicans JF - FREE RADICAL BIOLOGY AND MEDICINE J2 - FREE RADICAL BIO MED VL - 176 PY - 2021 SP - 222 EP - 227 PG - 6 SN - 0891-5849 DO - 10.1016/j.freeradbiomed.2021.09.020 UR - https://m2.mtmt.hu/api/publication/32282187 ID - 32282187 N1 - Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary Export Date: 09 January 2024; Cited By: 4; Correspondence Address: P. Nagy; Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; email: nagyp@med.unideb.hu; V. Dombrádi; Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; email: dombradi@med.unideb.hu; CODEN: FRBME LA - English DB - MTMT ER - TY - JOUR AU - Jakab, Ágnes AU - Emri, Tamás AU - Csillag, Kinga Karola AU - Szabó, Anita AU - Nagy, Fruzsina AU - Baranyai, Edina AU - Sajtos, Zsófi AU - Géczi, Dóra Anikó AU - Antal, Károly AU - Kovács, Renátó AU - Szabó, Krisztina AU - Dombrádi, Viktor Béla AU - Pócsi, István TI - The Negative Effect of Protein Phosphatase Z1 Deletion on the Oxidative Stress Tolerance of Candida albicans Is Synergistic with Betamethasone Exposure JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 7 PY - 2021 IS - 7 PG - 23 SN - 2309-608X DO - 10.3390/jof7070540 UR - https://m2.mtmt.hu/api/publication/32120842 ID - 32120842 N1 - Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, 4032, Hungary Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Agilent Atomic Spectroscopy Partner Laboratory, Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, 4032, Hungary Department of Zoology, Faculty of Sciences, Eszterházy Károly University, Eger, 3300, Hungary Faculty of Pharmacy, University of Debrecen, Debrecen, 4032, Hungary Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Cited By :4 Export Date: 6 December 2022 Correspondence Address: Jakab, Á.; Department of Molecular Biotechnology and Microbiology, Hungary; email: jakab.agnes@science.unideb.hu Funding details: EFOP-3.6.1-16-2016-00022 Funding details: TKP2020-IKA-04 Funding details: University of California, UC Funding details: Newcastle University Funding details: European Commission, EC Funding details: Debreceni Egyetem, DE Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, K108989, K119494 Funding text 1: Funding: This research was funded by the European Union and the European Social (EFOP-3.6.1-16-2016-00022), the Hungarian National Research, Development and Innovation Office (NKFIH K108989 and K119494), and by the Thematic Excellence Programme (TKP2020-IKA-04) of the Ministry for Innovation and Technology in Hungary. Funding text 2: This research was funded by the European Union and the European Social (EFOP-3.6.1-16-2016-00022), the Hungarian National Research, Development and Innovation Office (NKFIH K108989 and K119494), and by the Thematic Excellence Programme (TKP2020-IKA-04) of the Ministry for Innovation and Technology in Hungary. Acknowledgments: Thanks are due to Alexander D. Johnson (Department of Biochemistry and Bio-physics, University of California, San Francisco, CA, USA) for providing the QMY23 (WT) C. albicans strain. We are grateful to Andrea Farkas Tankáné (Department of Medical Chemistry, University of Debrecen, Hungary) and Márta Rónaháti Tóthné (Department of Molecular Biotechnology and Microbiology, University of Debrecen, Hungary) for technical assistance. We thank Zsanett Andrásdi, Fanni Borus and Csilla Farkas (Department of Molecular Biotechnology and Microbiology, University of Debrecen, Hungary) for contributing to the experimental work, as well as Veronika Boczonadi (Biosciences Institute, Faculty of Medical Sciences, Newcastle University, UK) for English revision. AB - The glucocorticoid betamethasone (BM) has potent anti-inflammatory and immunosuppressive effects; however, it increases the susceptibility of patients to superficial Candida infections. Previously we found that this disadvantageous side effect can be counteracted by menadione sodium bisulfite (MSB) induced oxidative stress treatment. The fungus specific protein phosphatase Z1 (CaPpz1) has a pivotal role in oxidative stress response of Candida albicans and was proposed as a potential antifungal drug target. The aim of this study was to investigate the combined effects of CaPPZ1 gene deletion and MSB treatment in BM pre-treated C. albicans cultures. We found that the combined treatment increased redox imbalance, enhanced the specific activities of antioxidant enzymes, and reduced the growth in cappz1 mutant (KO) strain. RNASeq data demonstrated that the presence of BM markedly elevated the number of differentially expressed genes in the MSB treated KO cultures. Accumulation of reactive oxygen species, increased iron content and fatty acid oxidation, as well as the inhibiting ergosterol biosynthesis and RNA metabolic processes explain, at least in part, the fungistatic effect caused by the combined stress exposure. We suggest that the synergism between MSB treatment and CaPpz1 inhibition could be considered in developing of a novel combinatorial antifungal strategy accompanying steroid therapy. LA - English DB - MTMT ER - TY - JOUR AU - Dombrádi, Viktor Béla TI - Egy gombaspecifikus enzim, a protein foszfatáz Z jellemzése JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - XLV PY - 2021 IS - 2 SP - 22 EP - 35 PG - 14 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/32089120 ID - 32089120 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szabó, Krisztina AU - Miskei, Márton AU - Farkas, Ilona AU - Dombrádi, Viktor Béla TI - The phosphatome of opportunistic pathogen Candida species JF - FUNGAL BIOLOGY REVIEWS J2 - FUNG BIOL REV VL - 35 PY - 2021 SP - 40 EP - 51 PG - 12 SN - 1749-4613 DO - 10.1016/j.fbr.2020.12.002 UR - https://m2.mtmt.hu/api/publication/31815661 ID - 31815661 N1 - Funding Agency and Grant Number: Hungarian National Research, Development and Innovation Office [NKFIH K108989]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences; New National Excellence Program of the Ministry for Innovation and Technology, Hungary [UNKP-20-5] Funding text: The expert technical assistance of Ms. Andrea Tankane-Farkas is acknowledged. This work was supported by the Hungarian National Research, Development and Innovation Office (grant number NKFIH K108989). MM was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences, and by the New National Excellence Program of the Ministry for Innovation and Technology, Hungary (grant number UNKP-20-5). AB - Several Candida species, the best known example of which is Candida albicans, are opportunistic human pathogens that are responsible for frequent nosocomial infections. A worrisome aspect of the currently available treatments of candidemia is the steady development of resistance to antifungals among these potentially life threatening fungi. Under these circumstances the search for novel drug targets is a well justified research direction. We propose that the principles of signal transduction therapy by targeting protein phosphatases can be adopted as these enzymes carry out important physiological functions in Candida. We demonstrate that C. tropicalis, C. albicans, C. dubliniensis, and S. cerevisiae exhibit the largest repertoire of protein phosphatases among the investigated fungi. Together with other opportunistic pathogen Candida species and the members of the Saccharomycetales order, they expanded their phosphatome by repeated gene duplications. We noted that evolution generated a set of fungus specific phosphatases which can be targeted without the danger of causing undesirable side effects in the human body. Based on the conflicting criteria of effectiveness and selectivity, we identified and characterized 7 phosphatases that are potent virulence determinants and may be utilized as potential antifungal drug targets. (C) 2020 The Authors. Published by Elsevier Ltd on behalf of British Mycological Society. LA - English DB - MTMT ER - TY - CONF AU - Jakab, Ágnes AU - Szabó, A AU - Emri, Tamás AU - Dombrádi, Viktor Béla AU - Pócsi, István ED - Tünde, Pusztahelyi ED - Levente, Czeglédi ED - Éva, Domokos-Szabolcsy ED - Tamás, Emri TI - The combined effect of the protein phosphatase Z1 gene deletion and oxidative stress on the gene expression of the glucocorticoid treated Candida albicans. T2 - 4th National Conference of Young Biotechnologists "FIBOK 2020" online conference : abstract book PB - Debreceni Egyetem C1 - Debrecen PY - 2020 SP - 79 UR - https://m2.mtmt.hu/api/publication/31927583 ID - 31927583 LA - Hungarian DB - MTMT ER - TY - CONF AU - Jakab, Ágnes AU - Emri, Tamás AU - Géczi, Dóra AU - Szabó, Anita AU - Antal, Károly AU - Szabó, Krisztina AU - Dombrádi, Viktor Béla AU - Pócsi, István TI - The deletion of the fungus specific Cappz1 phosphatase enhances the oxidative stress sensitivity of Candida albicans in the presence of Betamethasone T2 - A Magyar Mikrobiológiai Társaság 2020. évi Nagygyűlése és a XIV. Fermentációs Kollokvium : Absztraktfüzet PB - Magyar Mikrobiológiai Társaság (MMT) C1 - Budapest PY - 2020 SP - 15 EP - 16 PG - 1 UR - https://m2.mtmt.hu/api/publication/31799994 ID - 31799994 LA - Hungarian DB - MTMT ER - TY - CONF AU - Nagy-Köteles, Csaba AU - Barta, Endre AU - Szabó, Krisztina AU - Jakab, Ágnes AU - Emri, Tamás AU - Nagy, Tibor AU - Dombrádi, Viktor Béla AU - Pócsi, István TI - A Candida albicans allélspecifikus génexpressziójának elemzése bioinformatikai módszerekkel. T2 - Biotechnológia a Debreceni Egyetemen Tudományos Szimpózium PY - 2019 SP - 38 EP - 38 PG - 1 UR - https://m2.mtmt.hu/api/publication/31348954 ID - 31348954 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szabó, Krisztina AU - Jakab, Ágnes AU - Póliska, Szilárd AU - Petrényi, Katalin AU - Kovács, Katalin AU - Issa, Lama Hasan Bou AU - Emri, Tamás AU - Pócsi, István AU - Dombrádi, Viktor Béla TI - Deletion of the fungus specific protein phosphatase Z1 exaggerates the oxidative stress response in Candida albicans JF - BMC GENOMICS J2 - BMC GENOMICS VL - 20 PY - 2019 IS - 1 SN - 1471-2164 DO - 10.1186/s12864-019-6252-6 UR - https://m2.mtmt.hu/api/publication/30925993 ID - 30925993 N1 - Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary Genomic Medicine and Bioinformatics Core Facility, Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary Export Date: 09 January 2024; Cited By: 7; Correspondence Address: V. Dombrádi; Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; email: dombradi@med.unideb.hu; CODEN: BGMEE LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Krisztina AU - Jakab, Ágnes AU - Póliska, Szilárd AU - Petrényi, K AU - Kovács, K AU - Lama, Hasan Bou Issa AU - Emri, Tamás AU - Pócsi, István AU - Dombrádi, Viktor Béla TI - Synergistic action of protein phosphatase Z1 deletion and oxidative stress in the opportunistic pathogen Candida albicans JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 66 PY - 2019 IS - 2019 SP - 193 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/30748158 ID - 30748158 LA - English DB - MTMT ER -