TY - CONF AU - SPITZMÜLLER, ZS. AU - KISS, T. AU - KARÁCSONY-PÁLFI, X. AU - Pintye, Alexandra AU - Molnár, Orsolya AU - Matolcsi, Fruzsina AU - Németh Z., Márk AU - Kiss, Levente AU - Kovács, M. Gábor AU - Váczy, Kálmán Zoltán ED - Haltrich, Attila TI - A szőlőlisztharmat kórokozó (Erysiphe necator) sdhi-fungicidekkel szembeni rezisztenciája az Egri Borvidéken T2 - 70. Növényvédelmi Tudományos Napok C1 - Budapest T3 - Növényvédelmi Tudományos Napok, ISSN 0231-2956 PY - 2024 SP - 74 UR - https://m2.mtmt.hu/api/publication/34685715 ID - 34685715 LA - Hungarian DB - MTMT ER - TY - CONF AU - Molnár, Orsolya AU - Németh Z., Márk AU - Bozsik, Gábor AU - Seress, Diána AU - Kovács, M. Gábor AU - Szőcs, Gábor ED - Haltrich, Attila TI - Fuzáriumok magyarországi ciprusfélék rákbetegség tüneteit mutató hajtásaiból T2 - 70. Növényvédelmi Tudományos Napok C1 - Budapest T3 - Növényvédelmi Tudományos Napok, ISSN 0231-2956 PY - 2024 SP - 38 UR - https://m2.mtmt.hu/api/publication/34685628 ID - 34685628 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Pintye, Alexandra AU - Bacsó, Renáta AU - Kovács, M. Gábor TI - Trans-kingdom fungal pathogens infecting both plants and humans, and the problem of azole fungicide resistance JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 15 PY - 2024 SN - 1664-302X DO - 10.3389/fmicb.2024.1354757 UR - https://m2.mtmt.hu/api/publication/34579676 ID - 34579676 N1 - Centre for Agricultural Research, Plant Protection Institute, HUN-REN, Budapest, Hungary Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Budapest, Hungary Export Date: 12 March 2024 Correspondence Address: Pintye, A.; Centre for Agricultural Research, Hungary; email: pintye.alexandra@atk.hun-ren.hu AB - Azole antifungals are abundantly used in the environment and play an important role in managing fungal diseases in clinics. Due to the widespread use, azole resistance is an emerging global problem for all applications in several fungal species, including trans-kingdom pathogens, capable of infecting plants and humans. Azoles used in agriculture and clinics share the mode of action and facilitating cross-resistance development. The extensive use of azoles in the environment, e.g., for plant protection and wood preservation, contributes to the spread of resistant populations and challenges using these antifungals in medical treatments. The target of azoles is the cytochrome p450 lanosterol 14-α demethylase encoded by the CYP51 (called also as ERG11 in the case of yeasts) gene. Resistance mechanisms involve mainly the mutations in the coding region in the CYP51 gene, resulting in the inadequate binding of azoles to the encoded Cyp51 protein, or mutations in the promoter region causing overexpression of the protein. The World Health Organization (WHO) has issued the first fungal priority pathogens list (FPPL) to raise awareness of the risk of fungal infections and the increasingly rapid spread of antifungal resistance. Here, we review the main issues about the azole antifungal resistance of trans-kingdom pathogenic fungi with the ability to cause serious human infections and included in the WHO FPPL. Methods for the identification of these species and detection of resistance are summarized, highlighting the importance of these issues to apply the proper treatment. LA - English DB - MTMT ER - TY - JOUR AU - Finy, Péter AU - Jeppson, Mikael AU - Knapp, Dániel AU - Papp, Viktor AU - Albert, László AU - Ölvedi, István AU - Bóka, Károly AU - Varga, Dóra AU - Kovács, M. Gábor AU - Dima, Bálint TI - Exploring diversity within the genus Tulostoma (Basidiomycota, Agaricales) in the Pannonian sandy steppe: four fascinating novel species from Hungary JF - MYCOKEYS J2 - MYCOKEYS VL - 100 PY - 2023 SP - 153 EP - 170 PG - 18 SN - 1314-4057 DO - 10.3897/mycokeys.100.112458 UR - https://m2.mtmt.hu/api/publication/34411893 ID - 34411893 N1 - Funding Agency and Grant Number: New National Excellence Program of the Ministry for Culture and Innovation from the National Research, Development and Innovation Fund (Hungary); National Research, Development and Innovation Office (Hungary); Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences Funding text: New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund (Hungary); National Research, Development and Innovation Office (Hungary); Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. AB - Steppe vegetation on sandy soil in Hungary has recently been revealed as one of the hot spots in Europe for the stalked puffballs (genus Tulostoma ). In the framework of the taxonomic revision of gasteroid fungi in Hungary, four Tulostoma species are described here as new to science: T. dunense , T. hungaricum , T. sacchariolens and T. shaihuludii . The study is based on detailed macro- and micromorphological investigations (including light and scanning electron microscopy), as well as a three-locus phylogeny of nrDNA ITS, nrDNA LSU and tef1-α sequences. The ITS and LSU sequences generated from the type specimen of T. cretaceum are provided and this resolved partly the taxonomy of the difficult species complex of T. aff. cretaceum. LA - English DB - MTMT ER - TY - JOUR AU - Molnár, Anna AU - Knapp, Dániel AU - Lovas, Miklós AU - Tóth, Gergő AU - Boldizsár, Imre AU - Váczy, Kálmán Zoltán AU - Kovács, M. Gábor TI - Untargeted metabolomic analyses support the main phylogenetic groups of the common plant-associated Alternaria fungi isolated from grapevine (Vitis vinifera) JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-46020-3 UR - https://m2.mtmt.hu/api/publication/34281522 ID - 34281522 N1 - Centre for Research and Development, Eszterházy Károly Catholic University, Leányka utca 6, Eger, 3300, Hungary Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, 1117, Hungary Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre U. 9, Budapest, 1092, Hungary Department of Pharmacognosy, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary Plant Protection Institute, Centre for Agricultural Research, Budapest, 1525, Hungary Department of Forestry and Wood Technology, Linnaeus University, Växjö, Sweden Export Date: 7 February 2024 Correspondence Address: Molnár, A.; Centre for Research and Development, Leányka utca 6, Hungary; email: molnar.anna@uni-eszterhazy.hu AB - Alternaria , a cosmopolitan fungal genus is a dominant member of the grapevine ( Vitis vinifera ) microbiome. Several Alternaria species are known to produce a variety of secondary metabolites, which are particularly relevant to plant protection and food safety in field crops. According to previous findings, the majority of Alternaria species inhabiting grapevine belong to Alternaria sect. Alternaria . However, the phylogenetic diversity and secondary metabolite production of the distinct Alternaria species has remained unclear. In this study, our aim was to examine the genetic and metabolic diversity of endophytic Alternaria isolates associated with the above-ground tissues of the grapevine. Altogether, 270 Alternaria isolates were collected from asymptomatic leaves and grape clusters of different grapevine varieties in the Eger wine region of Hungary. After analyses of the nuclear ribosomal DNA internal transcribed spacer (ITS) and RNA polymerase second largest subunit ( rpb2 ) sequences, 170 isolates were chosen for further analyses. Sequences of the Alternaria major allergen gene ( Alt a 1 ), endopolygalacturonase ( endoPG ), OPA10-2, and KOG1058 were also included in the phylogenetic analyses. Identification of secondary metabolites and metabolite profiling of the isolates were performed using high-performance liquid chromatography (HPLC)–high-resolution tandem mass spectrometry (HR-MS/MS). The multilocus phylogeny results revealed two distinct groups in grapevine, namely A . alternata and the A . arborescens species complex (AASC). Eight main metabolites were identified in all collected Alternaria isolates, regardless of their affiliation to the species and lineages. Multivariate analyses of untargeted metabolites found no clear separations; however, a partial least squares-discriminant analysis model was able to successfully discriminate between the metabolic datasets from isolates belonging to the AASC and A. alternata . By conducting univariate analysis based on the discriminant ability of the metabolites, we also identified several features exhibiting large and significant variation between A. alternata and the AASC. The separation of these groups may suggest functional differences, which may also play a role in the functioning of the plant microbiome. LA - English DB - MTMT ER - TY - JOUR AU - Pintye, Alexandra AU - Németh Z., Márk AU - Molnár, Orsolya AU - Horváth, Áron AU - Matolcsi, Fruzsina AU - Bókony, Veronika AU - Spitzmüller, Zsolt AU - Pálfi, Xénia AU - Váczy, Kálmán Zoltán AU - Kovács, M. Gábor TI - Comprehensive analyses of the occurrence of a fungicide resistance marker and the genetic structure in Erysiphe necator populations JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 16 SN - 2045-2322 DO - 10.1038/s41598-023-41454-1 UR - https://m2.mtmt.hu/api/publication/34139263 ID - 34139263 N1 - Plant Protection Institute, HUN-REN Centre for Agricultural Research, Budapest, Hungary Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Budapest, Hungary Food and Wine Research Institute, Eszterházy Károly Catholic University, Eger, Hungary Export Date: 9 October 2023 Correspondence Address: Németh, M.Z.; Plant Protection Institute, Hungary; email: nemeth.mark@atk.hu Chemicals/CAS: Fungicides, Industrial Funding details: Magyar Tudományos Akadémia, MTA, BO/00221/21/4 Funding details: European Regional Development Fund, ERDF Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA Funding details: Magyarország Kormánya, C1792177, GINOP-2.3.2-15-2016-00061, KDP-2021 Funding text 1: Project no. FK142735 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the FK_22 funding scheme. This research was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences (awarded to Márk Z. Németh; BO/00221/21/4), and by the Széchenyi 2020 Programme, the European Regional Development Fund and the Hungarian Government (GINOP-2.3.2-15-2016-00061). Project no. C1792177 has been implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the KDP-2021 funding scheme. Open access funding provided by Centre for Agricultural Research. AB - Genetically distinct groups of Erysiphe necator , the fungus causing grapevine powdery mildew infect grapevine in Europe, yet the processes sustaining stable genetic differences between those groups are less understood. Genotyping of over 2000 field samples from six wine regions in Hungary collected between 2017 and 2019 was conducted to reveal E. necator genotypes and their possible differentiation. The demethylase inhibitor (DMI) fungicide resistance marker A495T was detected in all wine regions, in 16% of the samples. Its occurrence differed significantly among wine regions and grape cultivars, and sampling years, but it did not differ between DMI-treated and untreated fields. Multilocus sequence analyses of field samples and 59 in vitro maintained isolates revealed significant genetic differences among populations from distinct wine regions. We identified 14 E. necator genotypes, of which eight were previously unknown. In contrast to the previous concept of A and B groups, European E. necator populations should be considered genetically more complex. Isolation by geographic distance, growing season, and host variety influence the genetic structuring of E. necator , which should be considered both during diagnoses and when effective treatments are planned. LA - English DB - MTMT ER - TY - CONF AU - Molnár, Anna AU - Knapp, Dániel AU - Lovas, Miklós AU - Boldizsár, Imre AU - Tóth, Gergő AU - Váczy, Kálmán Zoltán AU - Kovács, M. Gábor ED - K., MÁRIALIGETI ED - O., DOBAY TI - TAXONOMIC AND METABOLIC CHARACTERIZATION OF ALTERNARIA SPECIES IN GRAPEVINE (VITIS VINIFERA) IN HUNGARY T2 - Abstracts of the 19th International Congress of the Hungarian Society for Microbiology PY - 2023 SP - 72 EP - 72 PG - 1 UR - https://m2.mtmt.hu/api/publication/34122754 ID - 34122754 LA - English DB - MTMT ER - TY - CONF AU - Seress, Diána AU - Molnár, Orsolya AU - Pintye, Alexandra AU - Matolcsi, Fruzsina AU - Bókony, Veronika AU - Horváth, Áron AU - Kovács, M. Gábor AU - Németh Z., Márk TI - Prevalence and co-occurrence of two fungicide resistance markers in Erysiphe necator T2 - 12th International Congress of Plant Pathology PY - 2023 UR - https://m2.mtmt.hu/api/publication/34113193 ID - 34113193 LA - English DB - MTMT ER - TY - JOUR AU - Gaber, Dalia A. AU - Berthelot, Charlotte AU - Blaudez, Damien AU - Kovács, M. Gábor AU - Franken, Philipp TI - Impact of dark septate endophytes on salt stress alleviation of tomato plants JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 14 PY - 2023 SN - 1664-302X DO - 10.3389/fmicb.2023.1124879 UR - https://m2.mtmt.hu/api/publication/34070613 ID - 34070613 N1 - Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren, Germany Erfurt Research Centre for Horticultural Crops, University of Applied Sciences, Erfurt, Germany Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt Université de Lorraine, CNRS, LIEC, Nancy, France CTIFL, Centre de Carquefou, Carquefou, France Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Budapest, Hungary Centre for Agricultural Research, Plant Protection Institute, Budapest, Hungary Institute of Microbiology, Friedrich Schiller University Jena, Jena, Germany Export Date: 21 July 2023 Correspondence Address: Gaber, D.A.; Leibniz-Institute of Vegetable and Ornamental CropsGermany; email: dalia.mahmoud@fh-erfurt.de Correspondence Address: Franken, P.; Leibniz-Institute of Vegetable and Ornamental CropsGermany; email: philipp.franken@fh-erfurt.de AB - Fungal endophytes can improve plant tolerance to abiotic stress conditions. Dark septate endophytes (DSEs) belong to phylogenetically non-related groups of root colonizing fungi among the Ascomycota with high melanin-producing activities. They can be isolated from roots of more than 600 plant species in diverse ecosystems. Still the knowledge about their interaction with host plants and their contribution to stress alleviation is limited. The current work aimed to test the abilities of three DSEs ( Periconia macrospinosa , Cadophora sp., Leptodontidium sp.) to alleviate moderate and high salt stress in tomato plants. By including an albino mutant, the role of melanin for the interaction with plants and salt stress alleviation could also be tested. P. macrospinosa and Cadophora sp. improved shoot and root growth 6 weeks after inoculation under moderate and high salt stress conditions. No matter how much salt stress was applied, macroelement (P, N, and C) contents were unaffected by DSE inoculation. The four tested DSE strains successfully colonized the roots of tomato, but the colonization level was clearly reduced in the albino mutant of Leptodontidium sp. Any difference in the effects on plant growth between the Leptodontidium sp. wild type strain and the albino mutant could, however, not be observed. These results show that particular DSEs are able to increase salt tolerance as they promote plant growth specifically under stress condition. Increased plant biomasses combined with stable nutrient contents resulted in higher P uptake in shoots of inoculated plants at moderate and high salt conditions and higher N uptake in the absence of salt stress in all inoculated plants, in P. macrospinosa -inoculated plants at moderate salt condition and in all inoculated plants except the albino mutants at high salt condition. In summary, melanin in DSEs seems to be important for the colonization process, but does not influence growth, nutrient uptake or salt tolerance of plants. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Gergő AU - Horváti, Kata AU - Mazákné Kraszni, Márta AU - Ausbüttel, Tim AU - Pályi, Bernadett AU - Kis, Zoltán AU - Mucsi, Zoltán AU - Kovács, M. Gábor AU - Bősze, Szilvia AU - Boldizsár, Imre TI - Arylnaphthalene Lignans with Anti-SARS-CoV-2 and Antiproliferative Activities from the Underground Organs of Linum austriacum and Linum perenne JF - JOURNAL OF NATURAL PRODUCTS J2 - J NAT PROD VL - 86 PY - 2023 IS - 4 SP - 672 EP - 682 PG - 11 SN - 0163-3864 DO - 10.1021/acs.jnatprod.2c00580 UR - https://m2.mtmt.hu/api/publication/33676920 ID - 33676920 N1 - Department of Pharmaceutical Chemistry, Semmelweis University, Hőgyes Endre u. 9, Budapest, 1092, Hungary MTA-TTK Lendület “Momentum” Peptide-Based Vaccines Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Magyar Tudósok krt 2, Budapest, 1117, Hungary Department of Pharmacognosy, Semmelweis University, Üllői út 26, Budapest, 1085, Hungary Department of Plant Anatomy, Institute of Biology, Eötvös Loránd University, Pázmány Péter sétány 1/C, Budapest, 1117, Hungary National Biosafety Laboratory, National Public Health Center, Albert Flórián út 2-6, Budapest, 1097, Hungary Femtonics Ltd., Tűzoltó u. 59, Budapest, 1094, Hungary ELKH-ELTE Research Group of Peptide Chemistry, Eötvös Loránd Research Network (ELKH), Eötvös Loránd University, Pázmány Péter sétány 1/A, Budapest, 1117, Hungary Export Date: 11 April 2023 CODEN: JNPRD Correspondence Address: Bősze, S.; ELKH-ELTE Research Group of Peptide Chemistry, Pázmány Péter sétány 1/A, Hungary; email: szilvia.bosze@ttk.elte.hu Correspondence Address: Boldizsár, I.; Department of Pharmacognosy, Üllői út 26, Hungary; email: boldizsar.imre@pharma.semmelweis-univ.hu LA - English DB - MTMT ER -