TY - JOUR AU - Bereczki, Katalin AU - Benke, Attila AU - Tóth, Endre György AU - Megyes, Melinda AU - Korponai, Kristóf AU - Szili Kovács, Tibor AU - Illés, Gábor AU - Lados, Botond Boldizsár AU - Márialigeti, Károly TI - Soil pH and Nutrient Content Sustain Variability of Soil Bacterial Community Structure and Activity after Forest Clear-Cutting JF - FORESTS J2 - FORESTS VL - 15 PY - 2024 IS - 8 PG - 21 SN - 1999-4907 DO - 10.3390/f15081284 UR - https://m2.mtmt.hu/api/publication/35144262 ID - 35144262 AB - Clear-cutting is the most robust intervention in a forest ecosystem, causing marked changes in ecosystem processes. Although the effects of forest harvesting have been widely investigated, comparative studies can provide vital supplementary information concerning specific fields, including changes in soil microbiota structure and functioning. Our study examined the soil bacterial community composition, diversity, and activity of a mixed pedunculate oak stand over three years after clear-cutting based on 16S rRNA sequencing and substrate-induced respiration data. In addition, we conducted a yearly comparison with a control oak stand already in the regeneration phase. According to our results, the forest harvest caused only limited changes in the diversity, structure, and activity of the soil bacterial community of the oak stand, suggesting that soil parameters influence the soil bacterial community structure and functioning more significantly than the cessation of forest cover. LA - English DB - MTMT ER - TY - JOUR AU - Bereczki, Katalin AU - Tóth, Endre György AU - Szili Kovács, Tibor AU - Megyes, Melinda AU - Korponai, Kristóf AU - Lados, Botond Boldizsár AU - Illés, Gábor AU - Benke, Attila AU - Márialigeti, Károly TI - Soil Parameters and Forest Structure Commonly Form the Microbiome Composition and Activity of Topsoil Layers in Planted Forests JF - MICROORGANISMS J2 - MICROORGANISMS VL - 12 PY - 2024 IS - 6 PG - 22 SN - 2076-2607 DO - 10.3390/microorganisms12061162 UR - https://m2.mtmt.hu/api/publication/34967523 ID - 34967523 AB - Soil bacterial communities play a remarkable role in nutrient cycling, significantly affecting soil organic material content, soil fertility, and, in an indirect way, plant succession processes. Conversely, vegetation type influences microbial soil life. The present study compared the bacterial microbiome composition, diversity and catabolic activity profile of topsoil samples collected under three different forest types (a twice-coppiced black locust stand, a young, naturally reforested, and a middle-aged mixed pedunculate oak stand) planted on former arable land in the early 20th century. Diversity indices determined during 16S ribosomal RNA sequencing-based metagenome analysis indicated that the black locust stand had the highest soil bacterial community diversity. At the phylum level, Acidobacteriota, Actinobacteriota, Proteobacteria, Verrucomicrobiota, Bacteroidota, and Gemmatimonadota were the most abundant taxa in the forest soils. Concerning soil parameters, redundancy analysis revealed that pH had the highest impact on bacterial community structure and pH, and soil organic carbon content on the samples’ respiration patterns. As for catabolic activity, the recently clearcut oak forest showed the lowest substrate-induced respiration, and citrate was the main driver for the inter-stand variability of microbial activity. Our results confirm that soil parameters and forest type influence the composition and functioning of the soil bacterial microbiome. LA - English DB - MTMT ER - TY - CHAP AU - Szécsy, Orsolya AU - Szili Kovács, Tibor AU - Dombos, Miklós AU - Szabó, Anita AU - Szűcs-Vásárhelyi, Nóra AU - Rékási, Márk ED - Bakacsi, Zsófia ED - Horel, Ágota ED - Mészáros, János ED - Rékási, Márk ED - Takács, Tünde Mária TI - Soil microbial activity correlates with texture in Hungarian soils T2 - Alternatives to Reduce Soil Degradation (ARSD2024) - Book of abstracts PB - HUN-REN Centre for Agricultural Research CY - Budapest SN - 9786155387128 PY - 2024 SP - 47 PG - 1 UR - https://m2.mtmt.hu/api/publication/34854546 ID - 34854546 LA - English DB - MTMT ER - TY - JOUR AU - Szili Kovács, Tibor AU - Takács, Tünde Mária TI - Microbial Biomass and Rhizosphere Soil Properties in Response to Heavy Metal-Contaminated Flooding JF - AGRICULTURE-BASEL J2 - AGRICULTURE-BASEL VL - 14 PY - 2024 IS - 5 PG - 15 SN - 2077-0472 DO - 10.3390/agriculture14050756 UR - https://m2.mtmt.hu/api/publication/34854313 ID - 34854313 AB - Mining and metallurgy are the main sources of soil contamination with harmful metals, posing a significant threat to human health and ecosystems. River floodplains in the vicinity of metal mines or industrial plants are often subject to flooding with sediments containing heavy metals, which can be harmful to the soil ecosystem. This study aimed to investigate the microbial properties of the soil at a metal-contaminated site and to determine the significant relationships between the biological and chemical properties of the soil. The study site was located near the village of Gyöngyösoroszi, in the Mátra mountain region of northwest Hungary. A phytoremediation experiment was conducted in a metal-polluted floodplain using willow and corn plantations. The soil basal respiration, substrate-induced respiration, soil microbial biomass carbon (MBC), acid phosphatase activities, and soil chemical properties were measured. The soil of the contaminated sites had significantly higher levels of As, Pb, Zn, Cu, Cd, and Ca, whereas the unpolluted sites had significantly higher levels of phosphorus and potassium. The substrate-induced respiration showed a positive correlation with MBC and negative correlations with the metabolic quotient (qCO2). The soil plasticity index and phosphorus showed a positive correlation with MBC, whereas salinity and the presence of Cd, Pb, Zn, As, and Cu showed a negative correlation. Acid phosphomonoesterase activity negatively correlated with the plant-available phosphorus content and MBC, but was positively correlated with the contents of toxic elements, including cadmium, lead, zinc, arsenic, and copper. This study found a significant correlation between the qCO2 and the toxic element content. This suggests that an enhanced metabolic quotient (qCO2), together with a decreased MBC/SOC ratio, could be used to indicate the harmful effect of soil contamination by heavy metals in floodplain soils. LA - English DB - MTMT ER - TY - JOUR AU - Mucsi, Márton AU - Kériné Borsodi, Andrea AU - Megyes, Melinda AU - Szili Kovács, Tibor TI - Response of the metabolic activity and taxonomic composition of bacterial communities to mosaically varying soil salinity and alkalinity JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 15 SN - 2045-2322 DO - 10.1038/s41598-024-57430-2 UR - https://m2.mtmt.hu/api/publication/34768902 ID - 34768902 N1 - Export Date: 3 May 2024 Correspondence Address: Szili-Kovács, T.; Institute for Soil Sciences, Herman Ottó út 15, Hungary; email: szili-kovacs.tibor@atk.hun-ren.hu Correspondence Address: Borsodi, A.K.; Department of Microbiology, Pázmány P. sétány 1/C, Hungary; email: borsodi.andrea@ttk.elte.hu AB - Soil salinity and sodicity is a worldwide problem that affects the composition and activity of bacterial communities and results from elevated salt and sodium contents. Depending on the degree of environmental pressure and the combined effect of other factors, haloalkalitolerant and haloalkaliphilic bacterial communities will be selected. These bacteria play a potential role in the maintenance and restoration of salt-affected soils; however, until recently, only a limited number of studies have simultaneously studied the bacterial diversity and activity of saline–sodic soils. Soil samples were collected to analyse and compare the taxonomic composition and metabolic activity of bacteria from four distinct natural plant communities at three soil depths corresponding to a salinity‒sodicity gradient. Bacterial diversity was detected using 16S rRNA gene Illumina MiSeq amplicon sequencing. Community-level physiological profiles (CLPPs) were analysed using the MicroResp™ method. The genus-level bacterial composition and CLPPs differed significantly in soils with different alkaline vegetation. The surface soil samples also significantly differed from the intermediate and deep soil samples. The results showed that the pH, salt content, and Na + content of the soils were the main edaphic factors influencing both bacterial diversity and activity. With salinity and pH, the proportion of the phylum Gemmatimonadota increased, while the proportions of Actinobacteriota and Acidobacteriota decreased. LA - English DB - MTMT ER - TY - CONF AU - Bereczki, Katalin AU - Benke, Attila AU - Tóth, Endre György AU - Megyes, Melinda AU - Korponai, Kristóf AU - Szili Kovács, Tibor AU - Illés, Gábor AU - Márialigeti, Károly ED - Czimber, Kornél TI - A fakitermelés hatása egy erdőtalaj talajbaktérium-közösségének szerkezetére és aktivitására T2 - Erdészeti Tudományos Konferencia Sopron, 2024. február 5-6. : Kivonatok Kötete PB - Soproni Egyetem Erdőmérnöki Kar C1 - Sopron PY - 2024 SP - 74 UR - https://m2.mtmt.hu/api/publication/34526238 ID - 34526238 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szili Kovács, Tibor AU - Mucsi, Márton AU - Füzy, Anna AU - Takács, Tünde Mária AU - Kériné Borsodi, Andrea TI - A talaj katabolikus aktivitás mintázatának elemzése mikrorespirációs (MicroResp™) módszerrel JF - AGROKÉMIA ÉS TALAJTAN J2 - AGROKÉMIA ÉS TALAJTAN VL - 72 PY - 2023 IS - 2 SP - 118 EP - 131 PG - 14 SN - 0002-1873 DO - 10.1556/0088.2023.00153 UR - https://m2.mtmt.hu/api/publication/34431713 ID - 34431713 AB - A talaj mikrobiális közösségének funkcionális diverzitása a talaj ökoszisztéma szolgáltatások jelentős részéhez hozzájárul, sok esetben meghatározó jelentőségű. Többféle kísérleti és elméleti megközelítés közül a katabolikus aktivitás-mintázat mikrorespirációs – MicroResp™ – módszerrel történő megközelítését mutatjuk be. A módszer a régebbről ismert szubsztrát-indukált respiráció több-szubsztrátos, mikrotiter lemez alapú kiterjesztése, amivel a talaj mikroba-közösség in-situ közösségi-szintű fiziológiai mintázata határozható meg. Mivel az egyes mikroorganizmusok szubsztrát-hasznosítása eltérő, a mikroba-közösség aktuális összetételétől, abundanciájától függően változó a szubsztrát hasznosítási mintázat egy-egy talajminta esetében. Az alkalmazott szubsztrátok köre tetszőleges, rendszerint egyszerű cukrok, aminosavak, aminok, karbonsavak. A módszer gyors, érzékeny, megbízható, ezért alkalmazása tervezett kísérletekben és talajmonitoring programokban egyaránt javasolható. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Füzy, Anna AU - Parádi, István AU - Kelemen, Bettina AU - Kovács, Ramóna AU - Cseresnyés, Imre AU - Szili Kovács, Tibor AU - Árendás, Tamás AU - Fodor, Nándor AU - Takács, Tünde Mária TI - Soil biological activity after a sixty-year fertilization practice in a wheat-maize crop rotation JF - PLOS ONE J2 - PLOS ONE VL - 18 PY - 2023 IS - 9 PG - 18 SN - 1932-6203 DO - 10.1371/journal.pone.0292125 UR - https://m2.mtmt.hu/api/publication/34165291 ID - 34165291 AB - This study aimed to survey the long-term effects of fertilization practices on the functional diversity of the soil microbiota. A 60-year fertilization experiment with mineral fertilizers, farmyard manure and combined treatments was sampled in two consecutive years in maize ( Zea mays L.) and wheat ( Triticum aestivum L.). Soil chemical properties, plant growth and physiological parameters were measured. The MicroResp TM method was applied to assess the community level physiological profiles (CLPPs) of the rhizosphere soil, and the arbuscular mycorrhizal fungal (AMF) colonization of the roots was determined. Samples were taken in the early vegetative stages, at flowering, and at harvest in both years. The measured parameters were analysed using multifactorial ANOVA to determine treatment effects, crop-dependent differences, and seasonality. PCA analysis was performed on the data matrix to reveal more complex correspondences, and Pearson’s product-moment correlation was used to confirm relationships between some of the measured soil and plant parameters. Fertilization treatments caused long-term changes in some biological parameters such as: MicroResp TM parameters, citrate utilization, total substrate-induced respiration value, and the ratio of utilization of amino acids and sugars. The rate of AMF colonization responded mainly to the plant nutrition status and the plant requirements, suggesting a plant-mediated effect in the case of mycorrhiza. Mineral nitrogen fertilization and soil acidification were found to be the main factors affecting the catabolic activity of soil microbiota, while AMF colonization responded to the balance of plant nutrition. LA - English DB - MTMT ER - TY - JOUR AU - Szili Kovács, Tibor TI - Üzenet az Agrokémia és Talajtan 72. évfolyamának olvasóihoz JF - AGROKÉMIA ÉS TALAJTAN J2 - AGROKÉMIA ÉS TALAJTAN VL - 72 PY - 2023 IS - 1 SP - 1 EP - 2 PG - 2 SN - 0002-1873 DO - 10.1556/0088.2023.00150 UR - https://m2.mtmt.hu/api/publication/34038342 ID - 34038342 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szili Kovács, Tibor AU - Takács, Tünde Mária TI - Advanced Research of Rhizosphere Microbial Activity. editorial TS - editorial JF - AGRICULTURE-BASEL J2 - AGRICULTURE-BASEL VL - 13 PY - 2023 IS - 4 PG - 4 SN - 2077-0472 DO - 10.3390/agriculture13040911 UR - https://m2.mtmt.hu/api/publication/33767371 ID - 33767371 AB - Soils are generally considered a complex and largely unexplored vital “black box” with thousands of microorganism taxa in their networks [...] LA - English DB - MTMT ER -