TY - JOUR AU - Varga, Erzsébet AU - Balázs, Viktória Lilla AU - Sándor, Viktor AU - Agócs, Attila AU - Nagy, Veronika AU - Király, Sándor Balázs AU - Kurtán, Tibor AU - Molnár, Péter AU - Deli, József TI - Carotenoid Composition of Telekia speciosa JF - PLANTS-BASEL J2 - PLANTS-BASEL VL - 12 PY - 2023 IS - 24 PG - 12 SN - 2223-7747 DO - 10.3390/plants12244116 UR - https://m2.mtmt.hu/api/publication/34429989 ID - 34429989 N1 - Department of Pharmacognosy and Phytotherapy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, Târgu Mureș, 540139, Romania Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, Rókus utca 2, Pécs, H-7624, Hungary Institute of Bioanalysis, Medical School, University of Pécs, Szigeti út 12, Pécs, H-7624, Hungary Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Szigeti út 12, Pécs, H-7624, Hungary Department of Organic Chemistry, Faculty of Sciences, University of Debrecen, Debrecen, H-4032, Hungary Export Date: 8 January 2024 Correspondence Address: Deli, J.; Department of Pharmacognosy, Rókus utca 2, Hungary; email: jozsef.deli@aok.pte.hu AB - The carotenoid composition of the flower of Telekia speciosa was investigated for the first time by HPLC-DAD-MS. In addition to the main carotenoid lutein and its geometrical isomers, 5,6-epoxy-carotenoids, namely violaxanthin, lutein 5,6-epoxide and antheraxanthin, were detected in larger amounts. In addition, β-carotene 5,6-epoxide and β-carotene 5,6,5′,6′-diepoxide were found, which occurs very rarely in plants. For unambigous identification, β-carotene 5,6-epoxide and β-carotene 5,6,5′,6′-diepoxide were prepared semisynthetically, and they were characterized by 1H and 13C NMR and HPLC-CD methods. LA - English DB - MTMT ER - TY - JOUR AU - Balázs, Viktória Lilla AU - Gulyás, Gergely AU - Nagy, Veronika AU - Zubay, Péter AU - Szabó, Krisztina AU - Sándor, Viktor AU - Agócs, Attila AU - Deli, József TI - Carotenoid Composition of Calendula officinalis Flowers with Identification of the Configuration of 5,8-Epoxy-carotenoids JF - ACS AGRICULTURAL SCIENCE & TECHNOLOGY J2 - ACS AGRIC SCI TECHNOL VL - 3 PY - 2023 IS - 11 SP - 1092 EP - 1102 PG - 11 SN - 2692-1952 DO - 10.1021/acsagscitech.3c00367 UR - https://m2.mtmt.hu/api/publication/34274821 ID - 34274821 N1 - Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, Rókus u. 2., Pécs, H-7624, Hungary Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Szigeti út 12, Pécs, 7624, Hungary Institute of Bioanalytics, Medical School, University of Pécs, Szigeti út 12., Pécs, H-7624, Hungary Department of Medicinal and Aromatic Plants, Institute of Sustainable Horticulture, Hungarian University of Agriculture and Life Sciences, Villányi str. 29-43., Budapest, H-1118, Hungary Export Date: 11 December 2023 Correspondence Address: Deli, J.; Department of Pharmacognosy, Rókus u. 2., Hungary; email: jozsef.deli@aok.pte.hu LA - English DB - MTMT ER - TY - JOUR AU - Pap, Ramóna AU - Pandur, Edina AU - Jánosa, Gergely AU - Horváth, Adrienn AU - Sipos, Katalin AU - Agócs, Attila AU - Deli, József TI - Lutein and its metabolites modulate inflammation, oxidative stress, and lipid peroxidation at glutamate-induced neurotoxicity JF - CAROTENOID SCIENCE J2 - CAROTENOID SCI VL - 25 PY - 2023 SP - 187 EP - 187 PG - 1 SN - 1880-5671 UR - https://m2.mtmt.hu/api/publication/34122466 ID - 34122466 LA - English DB - MTMT ER - TY - JOUR AU - Pap, Ramóna AU - Pandur, Edina AU - Jánosa, Gergely AU - Sipos, Katalin AU - Fritz, Ferenc Rómeó AU - Nagy, Tamás AU - Agócs, Attila AU - Deli, József TI - Protective Effects of 3′-Epilutein and 3′-Oxolutein against Glutamate-Induced Neuronal Damage JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 24 SN - 1661-6596 DO - 10.3390/ijms241512008 UR - https://m2.mtmt.hu/api/publication/34082313 ID - 34082313 N1 - ISSN:1422-0067 AB - Dietary lutein can be naturally metabolized to 3′-epilutein and 3′-oxolutein in the human body. The epimerization of lutein can happen in acidic pH, and through cooking, 3′-epilutein can be the product of the direct oxidation of lutein in the retina, which is also present in human serum. The 3′-oxolutein is the main oxidation product of lutein. Thus, the allylic oxidation of dietary lutein can result in the formation of 3′-oxolutein, which may undergo reduction either to revert to dietary lutein or epimerize to form 3′-epilutein. We focused on the effects of 3′-epilutein and 3′-oxolutein itself and on glutamate-induced neurotoxicity on SH-SY5Y human neuroblastoma cells to identify the possible alterations in oxidative stress, inflammation, antioxidant capacity, and iron metabolism that affect neurological function. ROS measurements were performed in the differently treated cells. The inflammatory state of cells was followed by TNFα, IL-6, and IL-8 cytokine ELISA measurements. The antioxidant status of the cells was determined by the total antioxidant capacity kit assay. The alterations of genes related to ferroptosis and lipid peroxidation were followed by gene expression measurements; then, thiol measurements were performed. Lutein metabolites 3′-epilutein and 3′-oxolutein differently modulated the effect of glutamate on ROS, inflammation, ferroptosis-related iron metabolism, and lipid peroxidation in SH-SY5Y cells. Our results revealed the antioxidant and anti-inflammatory features of 3′-epilutein and 3′-oxolutein as possible protective agents against glutamate-induced oxidative stress in SH-SY5Y cells, with greater efficacy in the case of 3′-epilutein. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Veronika AU - Agócs, Attila AU - Balázs, Viktória Lilla AU - Purger, Dragica AU - Filep, Rita AU - Sándor, Viktor AU - Turcsi, Erika Margit AU - Gulyás, Gergely AU - Deli, József TI - Lutein Isomers: Preparation, Separation, Structure Elucidation, and Occurrence in 20 Medicinal Plants JF - MOLECULES J2 - MOLECULES VL - 28 PY - 2023 IS - 3 PG - 18 SN - 1420-3049 DO - 10.3390/molecules28031187 UR - https://m2.mtmt.hu/api/publication/33597596 ID - 33597596 AB - Lutein and its cis-isomers occur in a lot of plants, including a variety of flowers. In this study, lutein isomers were produced via iodine-catalyzed isomerization, and four cis-isomers (9Z-, 9′Z-, 13Z-, and 13Z′) were isolated by means of column chromatography and semipreparative HPLC. The structures of the 9′Z- and 13′Z-isomers were elucidated via NMR measurements. These compounds were used as standards for the HPLC-DAD-MS determination of the carotenoid composition of the flowers of 20 plant species, in which lutein and its geometrical isomers are the main components. The flowers showed great variation in their cis- and trans-lutein content, and also in the presence or absence of other carotenoids, such as violaxanthin, neoxanthin, β-cryptoxanthin, and β-carotene. Some of the investigated flowers were found to be rich sources of lutein without zeaxanthin. LA - English DB - MTMT ER - TY - JOUR AU - Garai, János AU - Radnai, Balázs AU - Vámos, Eszter AU - Kovács, Dominika AU - Bagóné Vántus, Viola AU - Rumbus, Zoltán AU - Pákai, Eszter AU - Garami, András AU - Gulyás, Gergely AU - Agócs, Attila AU - Krekó, Marcell AU - Zaman, K. AU - Prókai, L. AU - Őrfi, László AU - Jakus, Péter AU - Lóránd, Tamás TI - Synthesis and evaluation of a new class of MIF-inhibitors in activated macrophage cells and in experimental septic shock in mice JF - EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY J2 - EUR J MED CHEM VL - 247 PY - 2023 PG - 11 SN - 0223-5234 DO - 10.1016/j.ejmech.2022.115050 UR - https://m2.mtmt.hu/api/publication/33546311 ID - 33546311 LA - English DB - MTMT ER - TY - JOUR AU - Pap, Ramóna AU - Pandur, Edina AU - Jánosa, Gergely AU - Sipos, Katalin AU - Nagy, Tamás AU - Agócs, Attila AU - Deli, József TI - Lutein Decreases Inflammation and Oxidative Stress and Prevents Iron Accumulation and Lipid Peroxidation at Glutamate-Induced Neurotoxicity JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 11 PY - 2022 IS - 11 PG - 25 SN - 2076-3921 DO - 10.3390/antiox11112269 UR - https://m2.mtmt.hu/api/publication/33251899 ID - 33251899 AB - The xanthophyll carotenoid lutein has been widely used as supplementation due to its protective effects in light-induced oxidative stress. Its antioxidant and anti-inflammatory features suggest that it has a neuroprotective role as well. Glutamate is a major excitatory neurotransmitter in the central nervous system (CNS), which plays a key role in regulating brain function. Excess accumulation of intracellular glutamate accelerates an increase in the concentration of reactive oxygen species (ROS) in neurons leading to glutamate neurotoxicity. In this study, we focused on the effects of glutamate on SH-SY5Y neuroblastoma cells to identify the possible alterations in oxidative stress, inflammation, and iron metabolism that affect the neurological function itself and in the presence of antioxidant lutein. First, ROS measurements were performed, and then catalase (CAT) and Superoxide Dismutase (SOD) enzyme activity were determined by enzyme activity assay kits. The ELISA technique was used to detect proinflammatory TNFα, IL-6, and IL-8 cytokine secretions. Alterations in iron uptake, storage, and release were followed by gene expression measurements and Western blotting. Total iron level detections were performed by a ferrozine-based iron detection method, and a heme assay kit was used for heme measurements. The gene expression toward lipid-peroxidation was determined by RT-PCR. Our results show glutamate changes ROS, inflammation, and antioxidant enzyme activity, modulate iron accumulation, and may initiate lipid peroxidation in SH-SY5Y cells. Meanwhile, lutein attenuates the glutamate-induced effects on ROS, inflammation, iron metabolism, and lipid peroxidation. According to our findings, lutein could be a beneficial, supportive treatment in neurodegenerative disorders. LA - English DB - MTMT ER - TY - JOUR AU - Agócs, Attila AU - Deli, József AU - Márk, László AU - Gallyas, Ferenc TI - Szemelvények a PTE ÁOK Biokémiai és Orvosi Kémiai Intézetében folyó tudományos kutatásokról JF - MAGYAR KÉMIAI FOLYÓIRAT - KÉMIAI KÖZLEMÉNYEK (1997-) J2 - MAGY KÉM FOLY KÉM KÖZL VL - 128 PY - 2022 IS - 2 SP - 86 EP - 91 PG - 6 SN - 1418-9933 DO - 10.24100/MKF.2022.02.86 UR - https://m2.mtmt.hu/api/publication/33090510 ID - 33090510 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Czett, Dalma AU - Böddi, Katalin AU - Nagy, Veronika AU - Takátsy, Anikó AU - Deli, József AU - Tone, Paul AU - Balogh, György Tibor AU - Vincze, Anna AU - Agócs, Attila TI - Synthesis, Pharmacokinetic Characterization and Antioxidant Capacity of Carotenoid Succinates and Their Melatonin Conjugates JF - MOLECULES J2 - MOLECULES VL - 27 PY - 2022 IS - 15 PG - 19 SN - 1420-3049 DO - 10.3390/molecules27154822 UR - https://m2.mtmt.hu/api/publication/33038711 ID - 33038711 AB - Carotenoid succinates were synthesized from hydroxy carotenoids and were coupled to a commercially available derivative of melatonin via amide bond for producing more powerful anti-oxidants and yet new hybrid lipophilic bifunctional molecules with additional therapeutic effects. The coupling reactions produced conjugates in acceptable to good yields. Succinylation increased the water solubility of the carotenoids, while the conjugation with melatonin resulted in more lipophilic derivatives. The conjugates showed self-assembly in aqueous medium and yielded relatively stable colloidal solutions in phosphate-buffered saline. Antioxidant behavior was measured with ABTS and the FRAP methods for the carotenoids, the carotenoid succinates, and the conjugates with melatonin. A strong dependence on the quality of the solvent was observed. TEAC values of the new derivatives in phosphate-buffered saline were found to be comparable to or higher than those of parent carotenoids, however, synergism was observed only in FRAP assays. LA - English DB - MTMT ER - TY - JOUR AU - Agócs, Attila AU - Turcsi, Erika Margit AU - Nagy, Veronika AU - Deli, József TI - Isolation and Analysis of Carotenoids in Hungary from Zechmeister until Today JF - PROCESSES J2 - PROCESSES VL - 10 PY - 2022 IS - 4 PG - 15 SN - 2227-9717 DO - 10.3390/pr10040707 UR - https://m2.mtmt.hu/api/publication/32780270 ID - 32780270 N1 - Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Szigeti u. 12, Pécs, H-7624, Hungary Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, Rókus u. 2, Pécs, H-7624, Hungary Export Date: 6 September 2023 Correspondence Address: Deli, J.; Department of Biochemistry and Medical Chemistry, Szigeti u. 12, Hungary; email: jozsef.deli@aok.pte.hu AB - László Zechmeister, one of the most important pioneers of carotenoid chemistry, died 50 years ago. He founded a carotenoid research group in Pécs (Hungary), which is the only place in the world where carotenoid research has been conducted continuously over the past 95 years. This review presents the life of Zechmeister and gives a summary about the evolution of the methods of analysis, isolation, and structure elucidation of carotenoids from the 1930s until today, based on the results of the research group founded by Zechmeister. LA - English DB - MTMT ER -