TY - JOUR AU - Asperger, Hannah AU - Stamm, Nadia AU - Gierke, Berthold AU - Pawlak, Michael AU - Hofmann, Ute AU - Zanger, Ulrich M. AU - Marton, Annamária AU - Katona, Róbert László AU - Buhala, Andrea AU - Vizler, Csaba AU - Cieslik, Jan-Philipp AU - Kovacevic, Zaklina AU - Richardson, Des R. AU - Ruckhaeberle, Eugen AU - Niederacher, Dieter AU - Fehm, Tanja AU - Neubauer, Hans AU - Ludescher, Marina TI - Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression (vol 22, 75, 2020) JF - BREAST CANCER RESEARCH J2 - BREAST CANCER RES VL - 23 PY - 2021 IS - 1 SN - 1465-5411 DO - 10.1186/s13058-020-01383-7 UR - https://m2.mtmt.hu/api/publication/31873459 ID - 31873459 AB - An amendment to this paper has been published and can be accessed via the original article. LA - English DB - MTMT ER - TY - JOUR AU - Asperger, Hannah AU - Stamm, Nadia AU - Gierke, Berthold AU - Pawlak, Michael AU - Hofmann, Ute AU - Zanger, Ulrich M. AU - Marton, Annamária AU - Katona, Róbert László AU - Buhala, Andrea AU - Vizler, Csaba AU - Cieslik, Jan-Philipp AU - Ruckhaeberle, Eugen AU - Niederacher, Dieter AU - Fehm, Tanja AU - Neubauer, Hans AU - Ludescher, Marina TI - Progesterone receptor membrane component 1 regulates lipid homeostasis and drives oncogenic signaling resulting in breast cancer progression JF - BREAST CANCER RESEARCH J2 - BREAST CANCER RES VL - 22 PY - 2020 IS - 1 SN - 1465-5411 DO - 10.1186/s13058-020-01312-8 UR - https://m2.mtmt.hu/api/publication/31397452 ID - 31397452 AB - Background: PGRMC1 (progesterone receptor membrane component 1) is a highly conserved heme binding protein, which is overexpressed especially in hormone receptor-positive breast cancer and plays an important role in breast carcinogenesis. Nevertheless, little is known about the mechanisms by which PGRMC1 drives tumor progression. The aim of our study was to investigate the involvement of PGRMC1 in cholesterol metabolism to detect new mechanisms by which PGRMC1 can increase lipid metabolism and alter cancer-related signaling pathways leading to breast cancer progression. Methods: The effect of PGRMC1 overexpression and silencing on cellular proliferation was examined in vitro and in a xenograft mouse model. Next, we investigated the interaction of PGRMC1 with enzymes involved in the cholesterol synthesis pathway such as CYP51, FDFT1, and SCD1. Further, the impact of PGRMC1 expression on lipid levels and expression of enzymes involved in lipid homeostasis was examined. Additionally, we assessed the role of PGRMC1 in key cancer-related signaling pathways including EGFR/HER2 and ER alpha signaling. Results: Overexpression of PGRMC1 resulted in significantly enhanced proliferation. PGRMC1 interacted with key enzymes of the cholesterol synthesis pathway, alters the expression of proteins, and results in increased lipid levels. PGRMC1 also influenced lipid raft formation leading to altered expression of growth receptors in membranes of breast cancer cells. Analysis of activation of proteins revealed facilitated ER alpha and EGFR activation and downstream signaling dependent on PGRMC1 overexpression in hormone receptor-positive breast cancer cells. Depletion of cholesterol and fatty acids induced by statins reversed this growth benefit. Conclusion: PGRMC1 may mediate proliferation and progression of breast cancer cells potentially by altering lipid metabolism and by activating key oncogenic signaling pathways, such as ER alpha expression and activation, as well as EGFR signaling. Our present study underlines the potential of PGRMC1 as a target for anti-cancer therapy. LA - English DB - MTMT ER - TY - JOUR AU - Fajka-Boja, Roberta AU - Szebeni, Gábor AU - Hunyadi-Gulyás Éva, Csilla AU - Puskás, László AU - Katona, Róbert László TI - Polyploid Adipose Stem Cells Shift the Balance of IGF1/IGFBP2 to Promote the Growth of Breast Cancer JF - FRONTIERS IN ONCOLOGY J2 - FRONT ONCOL VL - 10 PY - 2020 PG - 8 SN - 2234-943X DO - 10.3389/fonc.2020.00157 UR - https://m2.mtmt.hu/api/publication/31183285 ID - 31183285 N1 - Cited By :2 Export Date: 10 February 2021 Correspondence Address: Katona, R.L.; Artificial Chromosome and Stem Cell Research Laboratory, Hungary; email: katona.robert@brc.hu LA - English DB - MTMT ER - TY - JOUR AU - Gyukity-Sebestyén, Edina AU - Harmati, Mária AU - Dobra, Gabriella AU - Németh, István Balázs AU - Mihály, Johanna AU - Zvara, Ágnes AU - Hunyadi-Gulyás Éva, Csilla AU - Katona, Róbert László AU - Nagy, István AU - Horváth, Péter AU - Bálind, Árpád AU - Szkalisity, Ábel AU - Kovács, Mária AU - Pankotai, Tibor AU - Borsos, Barbara Nikolett AU - Erdélyi, Miklós AU - Szegletes, Zsolt AU - Veréb, Zoltán AU - Buzás, Edit Irén AU - Kemény, Lajos AU - Bíró, Tamás AU - Buzás, Krisztina TI - Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 10 PY - 2019 PG - 22 SN - 1664-3224 DO - 10.3389/fimmu.2019.02459 UR - https://m2.mtmt.hu/api/publication/30898978 ID - 30898978 N1 - Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Export Date: 14 January 2020 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of SciencesHungary; email: kr.buzas@gmail.com Chemicals/CAS: aggrecanase 1, 147172-61-0; alpha tubulin, 78769-62-7; carbonate dehydratase, 9001-03-0; cathepsin B, 9047-22-7; cathepsin D, 9025-26-7; lipocortin 5, 111237-10-6; mitogen activated protein kinase kinase, 142805-58-1; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6 Funding details: GINOP-2.2.1-15-2017-00052, 11493, GINOP-2.3.2-15-2016-00015 Funding details: NKP-19-4 Funding details: GINOP-2.3.2-15-2016-00036, KTIA_13_NAP-A-I/14 Funding text 1: The authors wish to thank G?bor Braunitzer and Csaba Vizler, for scientific discussion, Annam?ria Marton for technical issues and adaptation of MSC isolation protocol, Lilla Pint?r for technical support. Funding. This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-15-2017-00052, J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences ?NKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Export Date: 17 January 2020 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of SciencesHungary; email: kr.buzas@gmail.com Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Export Date: 28 January 2020 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of SciencesHungary; email: kr.buzas@gmail.com Chemicals/CAS: aggrecanase 1, 147172-61-0; alpha tubulin, 78769-62-7; carbonate dehydratase, 9001-03-0; cathepsin B, 9047-22-7; cathepsin D, 9025-26-7; lipocortin 5, 111237-10-6; mitogen activated protein kinase kinase, 142805-58-1; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6 Funding details: GINOP-2.2.1-15-2017-00052, 11493, GINOP-2.3.2-15-2016-00015 Funding details: NKP-19-4 Funding details: GINOP-2.3.2-15-2016-00036, KTIA_13_NAP-A-I/14 Funding text 1: The authors wish to thank G?bor Braunitzer and Csaba Vizler, for scientific discussion, Annam?ria Marton for technical issues and adaptation of MSC isolation protocol, Lilla Pint?r for technical support. Funding. This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-15-2017-00052, J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences ?NKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Export Date: 30 January 2020 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of SciencesHungary; email: kr.buzas@gmail.com Funding Agency and Grant Number: National Research, Development and Innovation Fund of Hungary under the NKFI-6-K funding scheme [11493, GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-152017-00052]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences [UNKP-19-4]; University of Szeged; Hungarian Brain Research Program [KTIA_13_NAP-A-I/14]; [GINOP-2.3.2-15-2016-00036] Funding text: This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-152017-00052, Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences UNKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Cited By :2 Export Date: 3 March 2020 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of SciencesHungary; email: kr.buzas@gmail.com Chemicals/CAS: aggrecanase 1, 147172-61-0; alpha tubulin, 78769-62-7; carbonate dehydratase, 9001-03-0; cathepsin B, 9047-22-7; cathepsin D, 9025-26-7; lipocortin 5, 111237-10-6; mitogen activated protein kinase kinase, 142805-58-1; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6 Funding details: GINOP-2.2.1-15-2017-00052, 11493, GINOP-2.3.2-15-2016-00015 Funding details: NKP-19-4 Funding details: GINOP-2.3.2-15-2016-00036, KTIA_13_NAP-A-I/14 Funding text 1: The authors wish to thank G?bor Braunitzer and Csaba Vizler, for scientific discussion, Annam?ria Marton for technical issues and adaptation of MSC isolation protocol, Lilla Pint?r for technical support. Funding. This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-15-2017-00052, J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences ?NKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Cited By :9 Export Date: 10 January 2021 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of SciencesHungary; email: kr.buzas@gmail.com Chemicals/CAS: aggrecanase 1, 147172-61-0; alpha tubulin, 78769-62-7; carbonate dehydratase, 9001-03-0; cathepsin B, 9047-22-7; cathepsin D, 9025-26-7; lipocortin 5, 111237-10-6; mitogen activated protein kinase kinase, 142805-58-1; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6; Programmed Cell Death 1 Receptor Funding details: Magyar Tudományos Akadémia, MTA Funding details: GINOP-2.2.1-15-2017-00052, 11493, GINOP-2.3.2-15-2016-00015, NKFI-6-K Funding details: GINOP-2.3.2-15-2016-00036, KTIA_13_NAP-A-I/14 Funding text 1: This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-15-2017-00052, János Bolyai Research Scholarship of the Hungarian Academy of Sciences ÚNKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Cited By :9 Export Date: 10 February 2021 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Hungary; email: kr.buzas@gmail.com Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Doctoral School of Interdisciplinary Sciences, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Laboratory of Proteomics Research, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Artificial Chromosome and Stem Cell Research Laboratory, Institute of Genetics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Sequencing Platform, Institute of Biochemistry, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Advanced Optical Imaging Group, Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Atomic Force Microscope Laboratory, Institute of Biophysics, Biological Research Centre of Hungarian Academy of Sciences, Szeged, Hungary MTA-SE Immuno-proteogenomics Extracellular Vesicle Research Group, Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry, University of Szeged, Szeged, Hungary Cited By :9 Export Date: 10 March 2021 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Hungary; email: kr.buzas@gmail.com Chemicals/CAS: aggrecanase 1, 147172-61-0; alpha tubulin, 78769-62-7; carbonate dehydratase, 9001-03-0; cathepsin B, 9047-22-7; cathepsin D, 9025-26-7; lipocortin 5, 111237-10-6; mitogen activated protein kinase kinase, 142805-58-1; protein bcl 2, 219306-68-0; protein kinase B, 148640-14-6; Programmed Cell Death 1 Receptor Funding details: NKP-19-4 Funding details: GINOP-2.3.2-15-2016-00036, KTIA_13_NAP-A-I/14 Funding details: 11493, GINOP-2.2.1-15-2017-00052, GINOP-2.3.2-15-2016-00015, NKFI-6-K Funding details: Magyar Tudományos Akadémia, MTA Funding text 1: The authors wish to thank G?bor Braunitzer and Csaba Vizler, for scientific discussion, Annam?ria Marton for technical issues and adaptation of MSC isolation protocol, Lilla Pint?r for technical support. Funding. This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-15-2017-00052, J?nos Bolyai Research Scholarship of the Hungarian Academy of Sciences ?NKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. Funding text 2: This work was in part supported by the National Research, Development and Innovation Fund of Hungary, financed under the NKFI-6-K funding scheme (11493 project), GINOP-2.3.2-15-2016-00015, GINOP-2.2.1-15-2017-00052, János Bolyai Research Scholarship of the Hungarian Academy of Sciences ÚNKP-19-4 New National Excellence Program of the Ministry for Innovation and Technology and University of Szeged Open Access Fund. The dSTORM experiments were funded by the Hungarian Brain Research Program (KTIA_13_NAP-A-I/14) and the GINOP-2.3.2-15-2016-00036. AB - Recently, it has been described that programmed cell death protein 1 (PD-1) overexpressing melanoma cells are highly aggressive. However, until now it has not been defined which factors lead to the generation of PD-1 overexpressing subpopulations. Here, we present that melanoma-derived exosomes, conveying oncogenic molecular reprogramming, induce the formation of a melanoma-like, PD-1 overexpressing cell population (mMSCPD-1+) from naïve mesenchymal stem cells (MSCs). Exosomes and mMSCPD-1+ cells induce tumor progression and expression of oncogenic factors in vivo. Finally, we revealed a characteristic, tumorigenic signaling network combining the upregulated molecules (e.g., PD-1, MET, RAF1, BCL2, MTOR) and their upstream exosomal regulating proteins and miRNAs. Our study highlights the complexity of exosomal communication during tumor progression and contributes to the detailed understanding of metastatic processes. LA - English DB - MTMT ER - TY - JOUR AU - Fajka-Boja, Roberta AU - Marton, Annamária AU - Tóth, Anna AU - Blazsó, Péter AU - Tubak, Vilmos AU - Bálint, Balázs AU - Nagy, István AU - Hegedűs, Zoltán AU - Vizler, Csaba AU - Katona, Róbert László TI - Adipose stem cells may promote cancer progression JF - RESEARCH OUTREACH J2 - RO VL - 2019 PY - 2019 SP - 6 EP - 9 PG - 4 SN - 2517-701X DO - 10.32907/RO-106-110113 UR - https://m2.mtmt.hu/api/publication/30693937 ID - 30693937 LA - English DB - MTMT ER - TY - JOUR AU - Fajka-Boja, Roberta AU - Marton, Annamária AU - Tóth, Anna AU - Blazsó, Péter AU - Tubak, Vilmos AU - Bálint, Balázs AU - Nagy, István AU - Hegedűs, Zoltán AU - Vizler, Csaba AU - Katona, Róbert László TI - Increased insulin-like growth factor 1 production by polyploid adipose stem cells promotes growth of breast cancer cells JF - BMC CANCER J2 - BMC CANCER VL - 18 PY - 2018 PG - 12 SN - 1471-2407 DO - 10.1186/s12885-018-4781-z UR - https://m2.mtmt.hu/api/publication/3411662 ID - 3411662 LA - English DB - MTMT ER - TY - JOUR AU - Naziroglu, M AU - Cig, B AU - Blum, W AU - Vizler, Csaba AU - Buhala, Andrea AU - Marton, Annamária AU - Katona, Róbert László AU - Jósvay, Katalin AU - Schwaller, B AU - Oláh, Zoltán AU - Pecze, László TI - Targeting breast cancer cells by MRS1477, a positive allosteric modulator of TRPV1 channels JF - PLOS ONE J2 - PLOS ONE VL - 12 PY - 2017 IS - 6 PG - 19 SN - 1932-6203 DO - 10.1371/journal.pone.0179950 UR - https://m2.mtmt.hu/api/publication/3250677 ID - 3250677 N1 - OA gold AB - There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction of acute pain and neurological inflammation. MRS1477, a dihydropyridine derivative acts as a positive allosteric modulator of TRPV1, if added together with capsaicin, but is ineffective, if given alone. Addition of MRS1477 evoked Ca2+ signals in MCF7 breast cancer cells, but not in primary breast epithelial cells. This indicates that MCF7 cells not only express functional TRPV1 channels, but also produce endogenous TRPV1 agonists. We investigated the effects of MRS1477 and capsaicin on cell viability, caspase-3 and -9 activities and reactive oxygen species production in MCF7 cells. The fraction of apoptotic cells was increased after 3 days incubation with capsaicin (10 mu M) paralleled by increased reactive oxygen species production and caspase activity. These effects were even more pronounced, when cells were incubated with MRS1477 (2 mu M) either alone or together with CAPS (10 mu M). Capsazepine, a TRPV1 blocker, inhibited both the effect of capsaicin and MRS1477. Whole-cell patch clamp recordings revealed that capsaicinevoked TRPV1-mediated current density levels were increased after 3 days incubation with MRS1477 (2 mu M). However, the tumor growth in MCF7 tumor-bearing immunodeficient mice was not significantly decreased after treatment with MRS1477 (10 mg/kg body weight, i.p., injection twice a week). In conclusion, in view of a putative in vivo treatment with MRS1477 or similar compounds further optimization is required. LA - English DB - MTMT ER - TY - JOUR AU - Buzás, Krisztina AU - Marton, Annamária AU - Vizler, Csaba AU - Gyukity-Sebestyén, Edina AU - Harmati, Mária AU - Nagy, Katalin AU - Zvara, Ágnes AU - Katona, Róbert László AU - Tubak, Vilmos AU - Endrész, Valéria AU - Németh, István Balázs AU - Oláh, Judit Magdolna AU - Vigh, László AU - Bíró, Tamás AU - Kemény, Lajos TI - Bacterial sepsis increases survival in metastatic melanoma: Chlamydophila pneumoniae induces macrophage polarization and tumor regression JF - JOURNAL OF INVESTIGATIVE DERMATOLOGY J2 - J INVEST DERMATOL VL - 136 PY - 2016 IS - 4 SP - 862 EP - 865 PG - 4 SN - 0022-202X DO - 10.1016/j.jid.2015.12.032 UR - https://m2.mtmt.hu/api/publication/2995020 ID - 2995020 N1 - Letter to the editor Terjedelme meghaladja a 10000 karaktert, táblázatot, ábrát tartalmaz, ezért besorolása rövid közlemény az MTA V. Osztályának ajánlása alapján. (BCS, SZTE admin4) LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Enikő AU - Fajka-Boja, Roberta AU - Kriston-Pál, Éva AU - Hornung, Ákos AU - Makra, Ildikó AU - Kudlik, Gyöngyi AU - Uher, Ferenc AU - Katona, Róbert László AU - Monostori, Éva AU - Czibula, Ágnes TI - Licensing by Inflammatory Cytokines Abolishes Heterogeneity of Immunosuppressive Function of Mesenchymal Stem Cell Population JF - STEM CELLS AND DEVELOPMENT J2 - STEM CELLS DEV VL - 24 PY - 2015 IS - 18 SP - 2171 EP - 2180 PG - 10 SN - 1547-3287 DO - 10.1089/scd.2014.0581 UR - https://m2.mtmt.hu/api/publication/2946565 ID - 2946565 N1 - Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, 62 Temesvári krt, Szeged, 6726, Hungary Stem Cell Biology Unit, National Blood Service, Budapest, Hungary Cited By :38 Export Date: 10 February 2021 CODEN: SCDTA Correspondence Address: Czibula, A.; Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, 62 Temesvári krt, Hungary AB - When mesenchymal stem cells (MSCs) are used for therapy of immunological pathologies, they get into an inflammatory environment, altering the effectiveness of the treatment. To establish the impact of environmental inflammatory factors on MSCs' immunofunction in the mirror of intrinsic heterogeneity of mouse MSC population, individual MSC clones were generated and characterized. Adipogenic but not osteogenic differentiation and pro-angiogenic activity of five independent MSC cell lines were similar. Regarding osteogenic differentiation, clones MSC3 and MSC6 exhibited poorer capacity than MSC2, MSC4, and MSC5. To study the immunosuppressive heterogeneity, in vitro and in vivo experiments have been carried out using T-cell proliferation assay and delayed-type hypersensitivity (DTH) response, respectively. A remarkable difference was found between the clones in their ability to inhibit T-cell proliferation in the following order: MSC2MSC5>MSC4>MSC3>>MSC6. Nevertheless, the differences between the immunosuppressive activities of the individual clones disappeared on pretreatment of the cells with pro-inflammatory cytokines, a procedure called licensing. Stimulation of all clones with IFN- and TNF- resulted in elevation of their inhibitory capability to a similar level. Nitric oxide (NO) and prostaglandin E2 (PGE2) were identified as major mediators of immunofunction of the MSC clones. The earlier findings were also supported by in vivo results. Without licensing, MSC2 inhibited DTH response, while MSC6 did not affect DTH response. In contrast, prestimulation of MSC6 with inflammatory cytokines resulted in strong suppression by this clone as well. Here, we have showed that MSC population is functionally heterogeneous in terms of immunosuppressive function; however, this variability is largely reduced under pro-inflammatory conditions. LA - English DB - MTMT ER - TY - JOUR AU - Katona, Róbert László TI - Human artificial chromosomes for future biomedicine. JF - CELL CYCLE J2 - CELL CYCLE VL - 14 PY - 2015 IS - 10 SP - 1494 EP - 1494 PG - 1 SN - 1538-4101 DO - 10.1080/15384101.2015.1032647 UR - https://m2.mtmt.hu/api/publication/2897512 ID - 2897512 LA - English DB - MTMT ER -