@article{MTMT:34774200,
	title = {Analysis of intact venom proteins with capillary zone electrophoresis - mass spectrometry},
	url = {https://m2.mtmt.hu/api/publication/34774200},
	author = {Vishwakarma, Gayatri and Andrási, Melinda and Szabó, Dávid Ruben and Hajdu, Péter Béla and Petrilla, Vladimir and Petrillová, Monika and Legath, Jaroslav and Gáspár, Attila},
	doi = {10.1016/j.microc.2024.110290},
	journal-iso = {MICROCHEM J},
	journal = {MICROCHEMICAL JOURNAL},
	volume = {200},
	unique-id = {34774200},
	issn = {0026-265X},
	year = {2024},
	eissn = {1095-9149}
}

@article{MTMT:34565926,
	title = {Activity of Potassium Channels in CD8+ T Lymphocytes: Diagnostic and Prognostic Biomarker in Ovarian Cancer?},
	url = {https://m2.mtmt.hu/api/publication/34565926},
	author = {Jusztus, Vivien and Medyouni, Ghofrane and Bagosi, Adrienn and Lampé, Rudolf and Panyi, György and Matolay, Orsolya and Maka, Eszter and Krasznai, Zoárd Tibor and Vörös, Orsolya and Hajdu, Péter Béla},
	doi = {10.3390/ijms25041949},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34565926},
	issn = {1661-6596},
	abstract = {CD8+ T cells play a role in the suppression of tumor growth and immunotherapy. Ion channels control the Ca2+-dependent function of CD8+ lymphocytes such as cytokine/granzyme production and tumor killing. Kv1.3 and KCa3.1 K+ channels stabilize the negative membrane potential of T cells to maintain Ca2+ influx through CRAC channels. We assessed the expression of Kv1.3, KCa3.1 and CRAC in CD8+ cells from ovarian cancer (OC) patients (n = 7). We found that the expression level of Kv1.3 was higher in patients with malignant tumors than in control or benign tumor groups while the KCa3.1 activity was lower in the malignant tumor group as compared to the others. We demonstrated that the Ca2+ response in malignant tumor patients is higher compared to control groups. We propose that altered Kv1.3 and KCa3.1 expression in CD8+ cells in OC could be a reporter and may serve as a biomarker in diagnostics and that increased Ca2+ response through CRAC may contribute to the impaired CD8+ function.},
	year = {2024},
	eissn = {1422-0067},
	pages = {1949},
	orcid-numbers = {Lampé, Rudolf/0000-0002-8230-7692; Panyi, György/0000-0001-6227-3301}
}

@article{MTMT:33674239,
	title = {Kv1.3-dependent immune system activation is regulated by KCNE4},
	url = {https://m2.mtmt.hu/api/publication/33674239},
	author = {Colomer-Molera, Magalí and Sastre, Daniel and Sole Codina, Laura and Vallejo-Gracia, Albert and Navarro Perez, Maria and Capera-Aragones, Jesusa and Estadella, Irene and Cassinelli, Silvia and Panyi, György and Hajdu, Péter Béla and Tamkun, Michael M. and Felipe, Antonio},
	doi = {10.1016/j.bpj.2022.11.302},
	journal-iso = {BIOPHYS J},
	journal = {BIOPHYSICAL JOURNAL},
	volume = {122},
	unique-id = {33674239},
	issn = {0006-3495},
	year = {2023},
	eissn = {1542-0086},
	pages = {14a},
	orcid-numbers = {Panyi, György/0000-0001-6227-3301}
}

@article{MTMT:33266468,
	title = {A Fluorescent Peptide Toxin for Selective Visualization of the Voltage-Gated Potassium Channel K V 1.3},
	url = {https://m2.mtmt.hu/api/publication/33266468},
	author = {Wai, Dorothy C. C. and Naseem, Muhammad Umair and Mocsár, Gábor and Babu Reddiar, Sanjeevini and Pan, Yijun and Csóti, Ágota and Hajdu, Péter Béla and Nowell, Cameron and Nicolazzo, Joseph A. and Panyi, György and Norton, Raymond S.},
	doi = {10.1021/acs.bioconjchem.2c00436},
	journal-iso = {BIOCONJUGATE CHEM},
	journal = {BIOCONJUGATE CHEMISTRY},
	volume = {33},
	unique-id = {33266468},
	issn = {1043-1802},
	year = {2022},
	eissn = {1520-4812},
	pages = {2197-2212},
	orcid-numbers = {Wai, Dorothy C. C./0000-0002-3871-9980; Naseem, Muhammad Umair/0000-0003-3849-6219; Pan, Yijun/0000-0002-1442-3333; Nicolazzo, Joseph A./0000-0002-0983-7152; Panyi, György/0000-0001-6227-3301; Norton, Raymond S./0000-0001-8893-0584}
}

@article{MTMT:33103847,
	title = {Editorial: Ion Channels and Transporters in Ca2+-Dependent Functions of Lymphocytes},
	url = {https://m2.mtmt.hu/api/publication/33103847},
	author = {Hajdu, Péter Béla and Wulff, Heike and Niemeyer, Barbara A. and Szabo, Ildiko},
	doi = {10.3389/fphys.2022.962110},
	journal-iso = {FRONT PHYSIOL},
	journal = {FRONTIERS IN PHYSIOLOGY},
	volume = {13},
	unique-id = {33103847},
	keywords = {CANCER; PHARMACOLOGY; ION CHANNELS; Autoimmunity; ion transporter; lymhocyte; Ca2+- signaling},
	year = {2022},
	eissn = {1664-042X}
}

@article{MTMT:32776876,
	title = {Role of C-Terminal Domain and Membrane Potential in the Mobility of Kv1.3 Channels in Immune Synapse Forming T Cells},
	url = {https://m2.mtmt.hu/api/publication/32776876},
	author = {Sebestyén, Veronika and Nagy, Éva and Mocsár, Gábor and Volkó, Julianna and Szilágyi, Orsolya and Kenesei, Ádám and Panyi, György and Tóth, Katalin and Hajdu, Péter Béla and Vámosi, György},
	doi = {10.3390/ijms23063313},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {23},
	unique-id = {32776876},
	issn = {1661-6596},
	abstract = {Voltage-gated Kv1.3 potassium channels are essential for maintaining negative membrane potential during T-cell activation. They interact with membrane-associated guanylate kinases (MAGUK-s) via their C-terminus and with TCR/CD3, leading to enrichment at the immunological synapse (IS). Molecular interactions and mobility may impact each other and the function of these proteins. We aimed to identify molecular determinants of Kv1.3 mobility, applying fluorescence correlation spectroscopy on human Jurkat T-cells expressing WT, C-terminally truncated (Delta C), and non-conducting mutants of mGFP-Kv1.3. Delta C cannot interact with MAGUK-s and is not enriched at the IS, whereas cells expressing the non-conducting mutant are depolarized. Here, we found that in standalone cells, mobility of Delta C increased relative to the WT, likely due to abrogation of interactions, whereas mobility of the non-conducting mutant decreased, similar to our previous observations on other membrane proteins in depolarized cells. At the IS formed with Raji B-cells, mobility of WT and non-conducting channels, unlike Delta C, was lower than outside the IS. The Kv1.3 variants possessing an intact C-terminus had lower mobility in standalone cells than in IS-engaged cells. This may be related to the observed segregation of F-actin into a ring-like structure at the periphery of the IS, leaving much of the cell almost void of F-actin. Upon depolarizing treatment, mobility of WT and Delta C channels decreased both in standalone and IS-engaged cells, contrary to non-conducting channels, which themselves caused depolarization. Our results support that Kv1.3 is enriched at the IS via its C-terminal region regardless of conductivity, and that depolarization decreases channel mobility.},
	year = {2022},
	eissn = {1422-0067},
	orcid-numbers = {Kenesei, Ádám/0000-0002-3950-7007; Panyi, György/0000-0001-6227-3301}
}

@article{MTMT:32487569,
	title = {Immune Synapse Residency of Orai1 Alters Ca2+ Response of T Cells},
	url = {https://m2.mtmt.hu/api/publication/32487569},
	author = {Vörös, Orsolya and Panyi, György and Hajdu, Péter Béla},
	doi = {10.3390/ijms222111514},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {22},
	unique-id = {32487569},
	issn = {1661-6596},
	year = {2021},
	eissn = {1422-0067},
	orcid-numbers = {Panyi, György/0000-0001-6227-3301}
}

@article{MTMT:32348330,
	title = {KCNE4-dependent functional consequences of Kv1.3-related leukocyte physiology},
	url = {https://m2.mtmt.hu/api/publication/32348330},
	author = {Vallejo-Gracia, Albert and Sastre, Daniel and Colomer-Molera, Magali and Sole, Laura and Navarro-Perez, Maria and Capera, Jesusa and Roig, Sara R. and Pedros-Gamez, Oriol and Estadella, Irene and Szilágyi, Orsolya and Panyi, György and Hajdu, Péter Béla and Felipe, Antonio},
	doi = {10.1038/s41598-021-94015-9},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {11},
	unique-id = {32348330},
	issn = {2045-2322},
	abstract = {The voltage-dependent potassium channel Kv1.3 plays essential roles in the immune system, participating in leukocyte activation, proliferation and apoptosis. The regulatory subunit KCNE4 acts as an ancillary peptide of Kv1.3, modulates K+ currents and controls channel abundance at the cell surface. KCNE4-dependent regulation of the oligomeric complex fine-tunes the physiological role of Kv1.3. Thus, KCNE4 is crucial for Ca2+-dependent Kv1.3-related leukocyte functions. To better understand the role of KCNE4 in the regulation of the immune system, we manipulated its expression in various leukocyte cell lines. Jurkat T lymphocytes exhibit low KCNE4 levels, whereas CY15 dendritic cells, a model of professional antigen-presenting cells, robustly express KCNE4. When the cellular KCNE4 abundance was increased in T cells, the interaction between KCNE4 and Kv1.3 affected important T cell physiological features, such as channel rearrangement in the immunological synapse, cell growth, apoptosis and activation, as indicated by decreased IL-2 production. Conversely, ablation of KCNE4 in dendritic cells augmented proliferation. Furthermore, the LPS-dependent activation of CY15 cells, which induced Kv1.3 but not KCNE4, increased the Kv1.3-KCNE4 ratio and increased the expression of free Kv1.3 without KCNE4 interaction. Our results demonstrate that KCNE4 is a pivotal regulator of the Kv1.3 channelosome, which fine-tunes immune system physiology by modulating Kv1.3-associated leukocyte functions.},
	year = {2021},
	eissn = {2045-2322},
	orcid-numbers = {Capera, Jesusa/0000-0002-8123-7725; Panyi, György/0000-0001-6227-3301}
}

@article{MTMT:31813030,
	title = {Immunomagnetic separation is a suitable method for electrophysiology and ion channel pharmacology studies on T cells},
	url = {https://m2.mtmt.hu/api/publication/31813030},
	author = {Tajti, Gábor and Szántó, Gábor Tibor and Csóti, Ágota and Racz, G. and Evaristo, C. and Hajdu, Péter Béla and Panyi, György},
	doi = {10.1080/19336950.2020.1859753},
	journal-iso = {CHANNELS},
	journal = {CHANNELS},
	volume = {15},
	unique-id = {31813030},
	issn = {1933-6950},
	abstract = {Ion channels play pivotal role in the physiological and pathological function of immune cells. As immune cells represent a functionally diverse population, subtype-specific functional studies, such as single-cell electrophysiology require proper subset identification and separation. Magnetic-activated cell sorting (MACS) techniques provide an alternative to fluorescence-activated cell sorting (FACS), however, the potential impact of MACS-related beads on the biophysical and pharmacological properties of the ion channels were not studied yet. We studied the aforementioned properties of the voltage-gated Kv1.3 K+ channel in activated CD4+ T-cells as well as the membrane capacitance using whole-cell patch-clamp following immunomagnetic positive separation, using the REAlease® kit. This kit allows three experimental configurations: bead-bound configuration, bead-free configuration following the removal of magnetic beads, and the label-free configuration following removal of CD4 recognizing antibody fragments. As controls, we used FACS separation as well as immunomagnetic negative selection. The membrane capacitance and of the biophysical parameters of Kv1.3 gating, voltage-dependence of steady-state activation and inactivation kinetics of the current were not affected by the presence of MACS-related compounds on the cell surface. We found subtle differences in the activation kinetics of the Kv1.3 current that could not be explained by the presence of MACS-related compounds. Neither the equilibrium block of Kv1.3 by TEA+ or charybdotoxin (ChTx) nor the kinetics of ChTx block are affected by the presence of the magnetics beads on the cell surface. Based on our results MACS is a suitable method to separate cells for studying ion channels in non-excitable cells, such as T-lymphocytes. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.},
	keywords = {ARTICLE; CHARYBDOTOXIN; human; cell separation; Electrophysiology; physiology; Angiography; Flow Cytometry; Immunoprecipitation; human cell; ion channel; immunocompetent cell; T lymphocyte; Cell viability; potassium channel; CD4+ T lymphocyte; blood vessel reactivity; peripheral blood mononuclear cell; hyperpolarization; fluorescence activated cell sorting; immunomagnetic separation; immunomagnetic separation; density gradient centrifugation; membrane steady potential; Kv1.3; FACS; fluorescence-activated cell sorting; MACS; Magnetic-activated cell sorting; cd4+ T-cell},
	year = {2021},
	eissn = {1933-6969},
	pages = {53-66},
	orcid-numbers = {Panyi, György/0000-0001-6227-3301}
}

@{MTMT:31672852,
	title = {KCa1.1 and Kv10.1 K+ channels are functionally expressed in chorion-derived mesenchymal stem cells},
	url = {https://m2.mtmt.hu/api/publication/31672852},
	author = {Szántó, Gábor Tibor and Mészáros, Beáta and Hajdu, Péter Béla and Tajti, Gábor and Panyi, György},
	booktitle = {Proceedings of the EFOP-3.6.2-16-2017-00006 (LIVE LONGER) project},
	unique-id = {31672852},
	abstract = {Introduction:
		Multipotent mesenchymal stem cells (MSCs) can be found in nearly all tissues, including the placenta. Therapeutic application of placenta-derived MSCs is very promising, as this tissue type is more readily available then other sources of MSCs. The ion channel repertoire of chorion-derived MSCs (cMSC) are far less understood compared to their bone marrow-derived MSC counterparts. The mRNA expression of ion channels has already been examined in our group (Mészáros et al), so our aim in this study was to determine ion channels that are functionally expressed in cMSCs.
		Methods:
		Ionic current measurements were carried out using the patch-clamp technique in whole-cell configuration. For pharmacological investigations a gravity-controlled perfusion system was used. 
		Results:
		A TTX sensitive sodium current was measured in cMSCs. This Na+ current was insensitive to the Nav1.7-specific inhibitor, ProTx-II. The outward K+ current was produced by multiple ion channels. The expression of Kv10.1 and KCa1.1 channels were confirmed based on either their characteristic biophysical properties or pharmacological features (i.e using the Kv10.1 inhibitor astemizole, or the subunit independent KCa1.1 blocker paxilline). Moreover, the expression of KCa1.1 associated β-subunits (β1, β3b and β3d) were identified on cMSCs using -subunit dependent inhibitor (iberiotoxin) and activators (arachidonic and lithocholic acid). 
		Conclusions:
		Our results strongly suggest that Na+ and K+ channels are functionally expressed in cMSCs, similar to their bone marrow-derived MSC counterparts. Pharmacological targeting of these channels may serve as a tool to alter cMSC functions.
		Acknowledgements: This work was supported by EFOP 3.6.2-16-2017-00006 (LIVE LONGER).},
	year = {2020},
	pages = {ISBN:9789633067642},
	orcid-numbers = {Panyi, György/0000-0001-6227-3301}
}