TY - JOUR AU - Varga, Viktor TI - Disentangling the Hippocampal Projectome JF - NEUROSCIENCE BULLETIN J2 - NEUROSCI BULL PY - 2024 SN - 1673-7067 DO - 10.1007/s12264-024-01255-6 UR - https://m2.mtmt.hu/api/publication/35133615 ID - 35133615 LA - English DB - MTMT ER - TY - JOUR AU - Berki, Péter AU - Cserép, Csaba AU - Környei, Zsuzsanna AU - Pósfai, Balázs AU - Cserépné Szabadits, Eszter AU - Domonkos, Andor AU - Kellermayer, Anna AU - Nyerges, Miklós AU - Wei, Xiaofei AU - Mody, Istvan AU - Kunihiko, Araki AU - Beck, Heinz AU - Kaikai, He AU - Ya, Wang AU - Lénárt, Nikolett AU - Wu, Zhaofa AU - Jing, Miao AU - Li, Yulong AU - Gulyás, Attila AU - Dénes, Ádám TI - Microglia contribute to neuronal synchrony despite endogenous ATP-related phenotypic transformation in acute mouse brain slices JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 15 PY - 2024 IS - 1 PG - 24 SN - 2041-1723 DO - 10.1038/s41467-024-49773-1 UR - https://m2.mtmt.hu/api/publication/35058848 ID - 35058848 LA - English DB - MTMT ER - TY - JOUR AU - Hegedüs, Panna AU - Király, Bálint AU - Schlingloff, Dániel AU - Lyakhova, Victoria AU - Velencei, Anna AU - Szabó, Írisz AU - Mayer, Márton István AU - Zelenak, Zsofia AU - Nyíri, Gábor AU - Hangya, Balázs TI - Parvalbumin-expressing basal forebrain neurons mediate learning from negative experience JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 15 PY - 2024 IS - 1 PG - 20 SN - 2041-1723 DO - 10.1038/s41467-024-48755-7 UR - https://m2.mtmt.hu/api/publication/35050908 ID - 35050908 AB - Parvalbumin (PV)-expressing GABAergic neurons of the basal forebrain (BFPVNs) were proposed to serve as a rapid and transient arousal system, yet their exact role in awake behaviors remains unclear. We performed bulk calcium measurements and electrophysiology with optogenetic tagging from the horizontal limb of the diagonal band of Broca (HDB) while male mice were performing an associative learning task. BFPVNs responded with a distinctive, phasic activation to punishment, but showed slower and delayed responses to reward and outcome-predicting stimuli. Optogenetic inhibition during punishment impaired the formation of cue-outcome associations, suggesting a causal role of BFPVNs in associative learning. BFPVNs received strong inputs from the hypothalamus, the septal complex and the median raphe region, while they synapsed on diverse cell types in key limbic structures, where they broadcasted information about aversive stimuli. We propose that the arousing effect of BFPVNs is recruited by aversive stimuli to serve crucial associative learning functions. LA - English DB - MTMT ER - TY - JOUR AU - Sárkány, Barbara AU - Dávid, Csaba AU - Hortobágyi, Tibor AU - Gombás, Péter AU - Somogyi, Peter AU - Acsády, László AU - Viney, Tim J. TI - Early and selective localization of tau filaments to glutamatergic subcellular domains within the human anterodorsal thalamus JF - ACTA NEUROPATHOLOGICA J2 - ACTA NEUROPATHOL VL - 147 PY - 2024 IS - 1 PG - 24 SN - 0001-6322 DO - 10.1007/s00401-024-02749-3 UR - https://m2.mtmt.hu/api/publication/35050868 ID - 35050868 AB - Widespread cortical accumulation of misfolded pathological tau proteins (ptau) in the form of paired helical filaments is a major hallmark of Alzheimer’s disease. Subcellular localization of ptau at various stages of disease progression is likely to be informative of the cellular mechanisms involving its spread. Here, we found that the density of ptau within several distinct rostral thalamic nuclei in post-mortem human tissue ( n = 25 cases) increased with the disease stage, with the anterodorsal nucleus (ADn) consistently being the most affected. In the ADn, ptau-positive elements were present already in the pre-cortical (Braak 0) stage. Tau pathology preferentially affected the calretinin-expressing subpopulation of glutamatergic neurons in the ADn. At the subcellular level, we detected ptau immunoreactivity in ADn cell bodies, dendrites, and in a specialized type of presynaptic terminal that expresses vesicular glutamate transporter 2 (vGLUT2) and likely originates from the mammillary body. The ptau-containing terminals displayed signs of degeneration, including endosomal/lysosomal organelles. In contrast, corticothalamic axon terminals lacked ptau. The data demonstrate the involvement of a specific cell population in ADn at the onset of the disease. The presence of ptau in subcortical glutamatergic presynaptic terminals supports hypotheses about the transsynaptic spread of tau selectively affecting specialized axonal pathways. LA - English DB - MTMT ER - TY - JOUR AU - Hajnal, Boglárka Zsófia AU - Szabó, Johanna Petra AU - Tóth, Emília AU - Keller, Corey J AU - Wittner, Lucia AU - Mehta, Ashesh D AU - Erőss, Loránd AU - Ulbert, István AU - Fabó, Dániel AU - Entz, László TI - Intracortical mechanisms of single pulse electrical stimulation (SPES) evoked excitations and inhibitions in humans. JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 17 SN - 2045-2322 DO - 10.1038/s41598-024-62433-0 UR - https://m2.mtmt.hu/api/publication/35050690 ID - 35050690 AB - Cortico-cortical evoked potentials (CCEPs) elicited by single-pulse electric stimulation (SPES) are widely used to assess effective connectivity between cortical areas and are also implemented in the presurgical evaluation of epileptic patients. Nevertheless, the cortical generators underlying the various components of CCEPs in humans have not yet been elucidated. Our aim was to describe the laminar pattern arising under SPES evoked CCEP components (P1, N1, P2, N2, P3) and to evaluate the similarities between N2 and the downstate of sleep slow waves. We used intra-cortical laminar microelectrodes (LMEs) to record CCEPs evoked by 10 mA bipolar 0.5 Hz electric pulses in seven patients with medically intractable epilepsy implanted with subdural grids. Based on the laminar profile of CCEPs, the latency of components is not layer-dependent, however their rate of appearance varies across cortical depth and stimulation distance, while the seizure onset zone does not seem to affect the emergence of components. Early neural excitation primarily engages middle and deep layers, propagating to the superficial layers, followed by mainly superficial inhibition, concluding in a sleep slow wave-like inhibition and excitation sequence. LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Nikolett AU - Hádinger, Nóra AU - Tóth, Otília AU - Rácz, Gergely Attila AU - Pintér, Tímea AU - Gál, Zoltán AU - Urbán, Martin AU - Gócza, Elen AU - Hiripi, László AU - Acsády, László AU - Vértessy, Beáta (Grolmuszné) TI - Characterization of dUTPase expression in mouse postnatal development and adult neurogenesis JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 18 SN - 2045-2322 DO - 10.1038/s41598-024-63405-0 UR - https://m2.mtmt.hu/api/publication/34939308 ID - 34939308 N1 - Funding Agency and Grant Number: Eotvos Lorand University; National Research, Development and Innovation Fund of Hungary [K135231, K146890, FK137867, NKP-2018-1.2.1-NKP-2018-00005, 2022-1.2.2-TET-IPARI-UZ-2022-00003]; Ministry for Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [TKP2021-EGA-02]; European Union [RRF-2.3.1-21-2022-00004] Funding text: Open access funding provided by Eotvos Lorand University.This study was mainly supported by the National Research, Development and Innovation Fund of Hungary (K135231, K146890, FK137867, NKP-2018-1.2.1-NKP-2018-00005, 2022-1.2.2-TET-IPARI-UZ-2022-00003 to BGV), and the TKP2021-EGA-02 grant, implemented with the support provided by the Ministry for Innovation and Technology of Hungary from the National Research, Development and Innovation Fund. The funding by European Union project within the framework of the Artificial Intelligence National Laboratory RRF-2.3.1-21-2022-00004 (LA) is also acknowledged. AB - The enzyme dUTPase has an essential role in maintaining genomic integrity. In mouse, nuclear and mitochondrial isoforms of the enzyme have been described. Here we present the isoform-specific mRNA expression levels in different murine organs during development using RT-qPCR. In this study, we analyzed organs of 14.5-day embryos and of postnatal 2-, 4-, 10-week- and 13-month-old mice. We demonstrate organ-, sex- and developmental stage-specific differences in the mRNA expression levels of both isoforms. We found high mRNA expression level of the nuclear isoform in the embryo brain, and the expression level remained relatively high in the adult brain as well. This was surprising, since dUTPase is known to play an important role in proliferating cells, and mass production of neural cells is completed by adulthood. Thus, we investigated the pattern of the dUTPase protein expression specifically in the adult brain with immunostaining and found that dUTPase is present in the germinative zones, the subventricular and the subgranular zones, where neurogenesis occurs and in the rostral migratory stream where neuroblasts migrate to the olfactory bulb. These novel findings suggest that dUTPase may have a role in cell differentiation and indicate that accurate dTTP biosynthesis can be vital, especially in neurogenesis. LA - English DB - MTMT ER - TY - JOUR AU - Aldahabi, Mohammad AU - Neher, Erwin AU - Nusser, Zoltán TI - Different states of synaptic vesicle priming explain target cell type–dependent differences in neurotransmitter release JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 121 PY - 2024 IS - 18 PG - 10 SN - 0027-8424 DO - 10.1073/pnas.2322550121 UR - https://m2.mtmt.hu/api/publication/34833183 ID - 34833183 N1 - Export Date: 15 August 2024 CODEN: PNASA Correspondence Address: Neher, E.; Laboratory of Membrane Biophysics, Germany; email: eneher@mpinat.mpg.de Correspondence Address: Nusser, Z.; Laboratory of Cellular Neurophysiology, Hungary; email: nusser@koki.hu Chemicals/CAS: ketamine, 1867-66-9, 6740-88-1, 81771-21-3; xylazine, 23076-35-9, 7361-61-7; Neurotransmitter Agents Tradenames: Nikon Eclipse FN1, Nikon Manufacturers: Nikon Funding details: Max-Planck-Gesellschaft, MPG Funding details: European Research Council, ERC, ERC-AG 787157 Funding details: European Research Council, ERC Funding details: NAP3.0 Funding details: Deutsche Forschungsgemeinschaft, DFG, CRC 1286 Funding details: Deutsche Forschungsgemeinschaft, DFG Funding text 1: Z.N. is the recipient of a European Research Council Advanced Grant (ERC-AG 787157) and a Hungarian National Brain Research Program (NAP3.0) grant. E.N. is supported by the Max Planck Society and by the Deutsche Forschungsgemeinschaft (DFG) under CRC 1286. The financial support from these funding bodies is gratefully acknowledged. We would like to thank Drs. Judit Makara, Jason Rothman, and R. Angus Silver for their comments on the MS. Funding text 2: ACKNOWLEDGMENTS. Z.N. is the recipient of a European Research Council Advanced Grant (ERC-AG 787157) and a Hungarian National Brain Research Program (NAP3.0) grant. E.N. is supported by the Max Planck Society and by the Funding text 3: Deutsche Forschungsgemeinschaft (DFG) under CRC 1286.The financial support fromthesefundingbodiesisgratefullyacknowledged.WewouldliketothankDrs. Judit Makara,Jason Rothman,and R.Angus Silver for their comments on the MS. AB - Pronounced differences in neurotransmitter release from a given presynaptic neuron, depending on the synaptic target, are among the most intriguing features of cortical networks. Hippocampal pyramidal cells (PCs) release glutamate with low probability to somatostatin expressing oriens-lacunosum-moleculare (O-LM) interneurons (INs), and the postsynaptic responses show robust short-term facilitation, whereas the release from the same presynaptic axons onto fast-spiking INs (FSINs) is ~10-fold higher and the excitatory postsynaptic currents (EPSCs) display depression. The mechanisms underlying these vastly different synaptic behaviors have not been conclusively identified. Here, we applied a combined functional, pharmacological, and modeling approach to address whether the main difference lies in the action potential-evoked fusion or else in upstream priming processes of synaptic vesicles (SVs). A sequential two-step SV priming model was fitted to the peak amplitudes of unitary EPSCs recorded in response to complex trains of presynaptic stimuli in acute hippocampal slices of adult mice. At PC–FSIN connections, the fusion probability (P fusion ) of well-primed SVs is 0.6, and 44% of docked SVs are in a fusion-competent state. At PC–O-LM synapses, P fusion is only 40% lower (0.36), whereas the fraction of well-primed SVs is 6.5-fold smaller. Pharmacological enhancement of fusion by 4-AP and priming by PDBU was recaptured by the model with a selective increase of P fusion and the fraction of well-primed SVs, respectively. Our results demonstrate that the low fidelity of transmission at PC–O-LM synapses can be explained by a low occupancy of the release sites by well-primed SVs. LA - English DB - MTMT ER - TY - JOUR AU - Tresóné Takács, Virág AU - Bardóczi, Zsuzsanna AU - Orosz, Áron AU - Major, Ábel AU - Tar, Luca AU - Berki, Péter AU - Papp, Péter AU - Mayer, Márton István AU - Sebők, Hunor AU - Zsolt, Luca AU - Sós, Katalin Eszter AU - Káli, Szabolcs AU - Freund, Tamás AU - Nyíri, Gábor TI - Synaptic and dendritic architecture of different types of hippocampal somatostatin interneurons JF - PLOS BIOLOGY J2 - PLOS BIOL VL - 22 PY - 2024 IS - 3 PG - 54 SN - 1544-9173 DO - 10.1371/journal.pbio.3002539 UR - https://m2.mtmt.hu/api/publication/34749015 ID - 34749015 N1 - Laboratory of Cerebral Cortex Research, HUN-REN Institute of Experimental Medicine, Budapest, Hungary János Szentágothai Doctoral, School of Neurosciences, Semmelweis University, Budapest, Hungary Roska Tamás Doctoral, School of Sciences and Technology, Pázmány Péter Catholic University, Budapest, Hungary Cited By :1 Export Date: 1 August 2024 CODEN: PBLIB Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Hungary; email: nyiri@koki.hu Chemicals/CAS: somatostatin, 38916-34-6, 51110-01-1; Somatostatin AB - GABAergic inhibitory neurons fundamentally shape the activity and plasticity of cortical circuits. A major subset of these neurons contains somatostatin (SOM); these cells play crucial roles in neuroplasticity, learning, and memory in many brain areas including the hippocampus, and are implicated in several neuropsychiatric diseases and neurodegenerative disorders. Two main types of SOM-containing cells in area CA1 of the hippocampus are oriens-lacunosum-moleculare (OLM) cells and hippocampo-septal (HS) cells. These cell types show many similarities in their soma-dendritic architecture, but they have different axonal targets, display different activity patterns in vivo, and are thought to have distinct network functions. However, a complete understanding of the functional roles of these interneurons requires a precise description of their intrinsic computational properties and their synaptic interactions. In the current study we generated, analyzed, and make available several key data sets that enable a quantitative comparison of various anatomical and physiological properties of OLM and HS cells in mouse. The data set includes detailed scanning electron microscopy (SEM)-based 3D reconstructions of OLM and HS cells along with their excitatory and inhibitory synaptic inputs. Combining this core data set with other anatomical data, patch-clamp electrophysiology, and compartmental modeling, we examined the precise morphological structure, inputs, outputs, and basic physiological properties of these cells. Our results highlight key differences between OLM and HS cells, particularly regarding the density and distribution of their synaptic inputs and mitochondria. For example, we estimated that an OLM cell receives about 8,400, whereas an HS cell about 15,600 synaptic inputs, about 16% of which are GABAergic. Our data and models provide insight into the possible basis of the different functionality of OLM and HS cell types and supply essential information for more detailed functional models of these neurons and the hippocampal network. LA - English DB - MTMT ER - TY - JOUR AU - Varga, Viktor AU - Petersen, Peter AU - Zutshi, Ipshita AU - Huszar, Roman AU - Zhang, Yiyao AU - Buzsáki, György TI - Working memory features are embedded in hippocampal place fields JF - CELL REPORTS J2 - CELL REP VL - 43 PY - 2024 IS - 3 SP - 113807 SN - 2211-1247 DO - 10.1016/j.celrep.2024.113807 UR - https://m2.mtmt.hu/api/publication/34714189 ID - 34714189 LA - English DB - MTMT ER - TY - JOUR AU - Zsoldos, Márton AU - Major, Ábel TI - A láthatatlan diszbiózis JF - KONZÍLIUM J2 - KONZÍLIUM VL - 2023 PY - 2023 IS - június SP - 1 EP - 5 PG - 5 SN - 1416-1397 UR - https://m2.mtmt.hu/api/publication/35159077 ID - 35159077 N1 - ISSN: 2732-0812 AB - Az alábbiakban a fogorvosi ellátás során gyakran alkal- mazott antibiotikumok használatának gasztrointesztinális traktusra gyakorolt hatásaival foglalkozunk. Szeretnénk felhívni a klinikus kollégák figyelmét az antibiotikumok használatából adódó mellékhatásokra és azok elkerülé- sének, mérséklésének gyakorlati lehetőségeire. LA - Hungarian DB - MTMT ER -