@{MTMT:34794891, title = {Expression of nutrient sensing genes mediate the effect of dietary unpredictability in Japanese quails}, url = {https://m2.mtmt.hu/api/publication/34794891}, author = {Gebrehaweria Kidane, Reda and Ndunguru, Sawadi Fransisco and Csernus, Brigitta and Szabó, Csaba and Czeglédi, Levente and Lendvai, Ádám Zoltán}, booktitle = {II. Magyar Agrártudományi Doktoranduszok Szimpóziuma 2024}, unique-id = {34794891}, year = {2024}, pages = {27}, orcid-numbers = {Lendvai, Ádám Zoltán/0000-0002-8953-920X} } @article{MTMT:34786100, title = {Dietary restriction reveals sex-specific expression of the mTOR pathway genes in Japanese quails}, url = {https://m2.mtmt.hu/api/publication/34786100}, author = {Gebrehaweria Kidane, Reda and Ndunguru, Sawadi Fransisco and Csernus, Brigitta and Knop, Renáta and Lugata, James Kachungwa and Szabó, Csaba and Czeglédi, Levente and Lendvai, Ádám Zoltán}, doi = {10.1038/s41598-024-58487-9}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34786100}, issn = {2045-2322}, abstract = {Limited resources affect an organism’s physiology through the conserved metabolic pathway, the mechanistic target of rapamycin (mTOR). Males and females often react differently to nutritional limitation, but whether it leads to differential mTOR pathway expression remains unknown. Recently, we found that dietary restriction (DR) induced significant changes in the expression of mTOR pathway genes in female Japanese quails ( Coturnix japonica ). We simultaneously exposed 32 male and female Japanese quails to either 20%, 30%, 40% restriction or ad libitum feeding for 14 days and determined the expression of six key genes of the mTOR pathway in the liver to investigate sex differences in the expression patterns. We found that DR significantly reduced body mass, albeit the effect was milder in males compared to females. We observed sex-specific liver gene expression. DR downregulated mTOR expression more in females than in males. Under moderate DR, ATG9A and RPS6K1 expressions were increased more in males than in females. Like females, body mass in males was correlated positively with mTOR and IGF1, but negatively with ATG9A and RS6K1 expressions. Our findings highlight that sexes may cope with nutritional deficits differently and emphasise the importance of considering sexual differences in studies of dietary restriction.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Lendvai, Ádám Zoltán/0000-0002-8953-920X} } @article{MTMT:34785047, title = {Dietary restriction and life-history trade-offs: insights into mTOR pathway regulation and reproductive investment in Japanese quails}, url = {https://m2.mtmt.hu/api/publication/34785047}, author = {Gebrehaweria Kidane, Reda and Ndunguru, Sawadi Fransisco and Csernus, Brigitta and Tóthné Gulyás, Gabriella and Knop, Renáta and Szabó, Csaba and Czeglédi, Levente and Lendvai, Ádám Zoltán}, doi = {10.1242/jeb.247064}, journal-iso = {J EXP BIOL}, journal = {JOURNAL OF EXPERIMENTAL BIOLOGY}, unique-id = {34785047}, issn = {0022-0949}, abstract = {Resources are needed for growth, reproduction and survival, and organisms must trade-off limited resources among competing processes. Nutritional availability in organisms is sensed and monitored by nutrient-sensing pathways that can trigger physiological changes or alter gene expression. Previous studies have proposed that one such signalling pathway, the mechanistic target of rapamycin (mTOR), underpins a form of adaptive plasticity when individuals encounter constraints in their energy budget. Despite the fundamental importance of this process in evolutionary biology, how nutritional limitation is regulated through the expression of genes governing this pathway and its consequential effects on fitness remains understudied, particularly in birds. We used dietary restriction to simulate resource depletion and examined its effects on body mass, reproduction and gene expression in Japanese quails (Coturnix japonica). Quails were subjected to feeding 20%, 30%, 40% restriction levels or ad libitum for two weeks. All restricted groups exhibited reduced body mass, whereas reductions in the number and mass of eggs were observed only under more severe restrictions. Additionally, dietary restriction led to decreased expression of mTOR and insulin-like growth factor 1 (IGF1), whereas the ribosomal protein S6 kinase 1 (RPS6K1) and autophagy-related genes (ATG9A and ATG5) were upregulated. The pattern in which mTOR respond to restriction was similar to what has been seen in body mass. Regardless of the treatment, proportionally higher reproductive investment was associated with individual variation in mTOR expression. These findings reveal the connection between dietary intake and the expression of mTOR and related genes in this pathway.}, year = {2024}, eissn = {1477-9145}, orcid-numbers = {Lendvai, Ádám Zoltán/0000-0002-8953-920X} } @article{MTMT:34762677, title = {The updated genome of the Hungarian population of Aedes koreicus}, url = {https://m2.mtmt.hu/api/publication/34762677}, author = {Nagy, Nikoletta Andrea and Tóth, Gábor Endre and Kurucz, Kornélia and Kemenesi, Gábor and Laczkó, Levente}, doi = {10.1038/s41598-024-58096-6}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34762677}, issn = {2045-2322}, abstract = {Vector-borne diseases pose a potential risk to human and animal welfare, and understanding their spread requires genomic resources. The mosquito Aedes koreicus is an emerging vector that has been introduced into Europe more than 15 years ago but only a low quality, fragmented genome was available. In this study, we carried out additional sequencing and assembled and characterized the genome of the species to provide a background for understanding its evolution and biology. The updated genome was 1.1 Gbp long and consisted of 6099 contigs with an N50 value of 329,610 bp and a BUSCO score of 84%. We identified 22,580 genes that could be functionally annotated and paid particular attention to the identification of potential insecticide resistance genes. The assessment of the orthology of the genes indicates a high turnover at the terminal branches of the species tree of mosquitoes with complete genomes, which could contribute to the adaptation and evolutionary success of the species. These results could form the basis for numerous downstream analyzes to develop targets for the control of mosquito populations.}, keywords = {Aedes; functional annotation; genome assembly; Invasive mosquito; hybrid assembly; Tird generation sequencing}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Tóth, Gábor Endre/0000-0002-7201-9646; Kurucz, Kornélia/0000-0001-6190-1265; Kemenesi, Gábor/0000-0001-9775-3065} } @article{MTMT:34756167, title = {Embryonic methionine triggers post-natal developmental programming in Japanese quail}, url = {https://m2.mtmt.hu/api/publication/34756167}, author = {Ndunguru, Sawadi Fransisco and Gebrehaweria Kidane, Reda and Csernus, Brigitta and Knop, Renáta and Tóthné Gulyás, Gabriella and Szabó, Csaba and Czeglédi, Levente and Lendvai, Ádám Zoltán}, doi = {10.1007/s00360-024-01542-8}, journal-iso = {J COMP PHYSIOL B}, journal = {JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY}, unique-id = {34756167}, issn = {0174-1578}, abstract = {Embryonic development is one of the most sensitive and critical stages when maternal effects may influence the offspring’s phenotype. In birds and other oviparous species, embryonic development is confined to the eggs, therefore females must deposit resources into the eggs to prepare the offspring for the prevailing post-natal conditions. However, the mechanisms of such phenotypic adjustments remain poorly understood. We simulated a maternal nutritional transfer by injecting 1 mg of l -methionine solution into Japanese quail eggs before the onset of incubation. The increase in early methionine concentration in eggs activated the insulin/insulin-like signalling and mechanistic target of rapamycin (IIS/mTOR) signalling pathways and affected post-natal developmental trajectories. Chicks from methionine-supplemented eggs had higher expression of liver IGF1 and mTOR genes at hatching but were similar in size, and the phenotypic effects of increased growth became apparent only a week later and remained up to three weeks. Circulating levels of insulin-like growth factor-1 (IGF-1) and expression of ribosomal protein serine 6 kinase 1 ( RPS6K1), the mTOR downstream effector, were elevated only three weeks after hatching. These results show that specific nutritional cues may have phenotypic programming effects by sequentially activating specific nutrient-sensing pathways and achieving transgenerational phenotypic plasticity.}, year = {2024}, eissn = {1432-136X}, orcid-numbers = {Lendvai, Ádám Zoltán/0000-0002-8953-920X} } @article{MTMT:34752419, title = {Haemosporidian infections are more common in breeding shorebirds than in migrating shorebirds}, url = {https://m2.mtmt.hu/api/publication/34752419}, author = {Jones, William and Tóth, Zsófia and Khursanov, Viacheslav and Kisliakova, Nastassia and Krüger, Oliver and Székely, Tamás and Karlionova, Natalia and Pinchuk, Pavel and Chakarov, Nayden}, doi = {10.1111/ibi.13318}, journal-iso = {IBIS}, journal = {IBIS}, volume = {-}, unique-id = {34752419}, issn = {0019-1019}, abstract = {Migrating animals are thought to be important spillover sources for novel pathogens. Haemosporidians (malaria‐related parasites) are one such group of pathogens that commonly spillover into novel host communities if competent vectors are present. In birds, shorebirds (sandpipers, plovers and allies) perform some of the longest avian migrations, yet they are traditionally perceived as relatively free from haemosporidians. Although low prevalence fits several theories, such as effective immune responses or low exposure to vectors, few studies have been carried out in freshwater inland sites, where the vectors of haemosporidians (e.g. mosquitoes) are abundant, with a mixture of actively migrating (staging) and breeding hosts. Here we report the prevalence of three haemosporidian parasites, Haemoproteus , Leucocytozoon and Plasmodium , screened in 214 shorebirds from 15 species sampled in a freshwater marshland, southern Belarus. Contrary to most previous studies, we found that haemosporidians were frequent, with an overall prevalence in the community of 16.36%, including the locally breeding shorebirds (23.13%, 134 individuals of 10 species). However, actively migrating shorebirds had much lower prevalence (0.05%, 55 individuals of five species). We suggest that blood parasite infections are more common in shorebirds than currently acknowledged. Yet, actively migrating species may be free from haemosporidians or carry suppressed infections, leading to lower prevalence or even apparent absence in some species. Taken together, we theorize that a combination of sampling biases has driven our understanding of haemosporidian prevalence in shorebirds and future studies should take the migratory status of individuals into account when reporting prevalence. Furthermore, we argue that birds undergoing active migration may be less likely sources of spillover events than previously assumed.}, keywords = {INFECTION; community ecology; shorebird; Avian malaria; migration}, year = {2024}, eissn = {1474-919X}, orcid-numbers = {Jones, William/0000-0002-3058-0072} } @article{MTMT:34680944, title = {In ovo feeding of methionine affects antioxidant status and growth-related gene expression of TETRA SL and Hungarian indigenous chicks}, url = {https://m2.mtmt.hu/api/publication/34680944}, author = {Lugata, James Kachungwa and Ndunguru, Sawadi Fransisco and Gebrehaweria Kidane, Reda and Tóthné Gulyás, Gabriella and Knop, Renáta and Oláh, János and Czeglédi, Levente and Szabó, Csaba}, doi = {10.1038/s41598-024-54891-3}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34680944}, issn = {2045-2322}, abstract = {Methionine (Met) plays a substantial role in poultry due to its involvement in several pathways, including enhancing antioxidant status and improving growth performance and health status. This study examined how in ovo feeding of Met affects hatching performance, antioxidant status, and hepatic gene expression related to growth and immunity in the TETRA-SL LL hybrid (TSL) commercial layer and Hungarian partridge colored hen (HPC) indigenous genotypes. The eggs were injected with saline, DL-Met, and L-Met on 17.5 days of embryonic development. The results showed that the in ovo feeding of DL-Met significantly increased the hatching weight and ferric reducing the ability of the plasma (FRAP) compared with L-Met. The in ovo feeding of either Met source enhanced the liver health and function and hepatic antioxidant status of the chicks. The genotype`s differences were significant; the TSL genotype had better hatching weight, an antioxidant defense system, and downregulated growth-related gene expression than the HPC genotype. In ovo feeding of either Met source enhanced the chicks' health status and antioxidant status, and DL-Met improved the hatching weight of the chicks more than L-Met. Genotype differences were significantly evident in the responses of growth performance, antioxidant status, blood biochemical parameters, and gene expression to Met sources.}, year = {2024}, eissn = {2045-2322} } @article{MTMT:34635507, title = {The draft genome of Spiraea crenata L. (Rosaceae) – the first complete genome in tribe Spiraeeae}, url = {https://m2.mtmt.hu/api/publication/34635507}, author = {Laczkó, Levente and Jordán, Sándor and Póliska, Szilárd and Rácz, Hanna Viktória and Nagy, Nikoletta Andrea and Molnár, V. Attila and Sramkó, Gábor}, doi = {10.1038/s41597-024-03046-0}, journal-iso = {SCI DATA}, journal = {SCIENTIFIC DATA}, volume = {11}, unique-id = {34635507}, abstract = {Spiraea crenata L. is a deciduous shrub distributed across the Eurasian steppe zone. The species is of cultural and horticultural importance and occurs in scattered populations throughout its westernmost range. Currently, there is no genomic information on the tribe of Spiraeeae. Therefore we sequenced and assembled the whole genome of S. crenata using second- and third-generation sequencing and a hybrid assembly approach to expand genomic resources for conservation and support research on this horticulturally important lineage. In addition to the organellar genomes (the plastome and the mitochondrion), we present the first draft genome of the species with an estimated size of 220 Mbp, an N50 value of 7.7 Mbp, and a BUSCO score of 96.0%. Being the first complete genome in tribe Spiraeeae, this may not only be the first step in the genomic study of a rare plant but also a contribution to genomic resources supporting the study of biodiversity and evolutionary history of Rosaceae.}, keywords = {whole genome; genome assembly; non-model plants}, year = {2024}, eissn = {2052-4463}, orcid-numbers = {Sramkó, Gábor/0000-0001-8588-6362} } @article{MTMT:34633164, title = {Synergy between systematics, biogeography and biodiversity: an honorary volume celebrating the achievements of professor Zoltán S. Varga}, url = {https://m2.mtmt.hu/api/publication/34633164}, author = {Sramkó, Gábor and Barta, Zoltán and Székely, Tamás}, doi = {10.1007/s42977-024-00207-5}, journal-iso = {BIOL FUTURA}, journal = {BIOLOGIA FUTURA}, volume = {75}, unique-id = {34633164}, issn = {2676-8615}, year = {2024}, eissn = {2676-8607}, pages = {1-5}, orcid-numbers = {Sramkó, Gábor/0000-0001-8588-6362; Barta, Zoltán/0000-0002-7121-9865} } @{MTMT:34632723, title = {The prenatal window: Influence of leucine on growth and development of Japanese quail}, url = {https://m2.mtmt.hu/api/publication/34632723}, author = {Ndunguru, Sawadi Fransisco and Gebrehaweria Kidane, Reda and Csernus, Brigitta and Szabó, Csaba and Lendvai, Ádám Zoltán and Czeglédi, Levente}, booktitle = {II. Magyar Agrártudományi Doktoranduszok Szimpóziuma 2024}, unique-id = {34632723}, year = {2024}, pages = {25}, orcid-numbers = {Lendvai, Ádám Zoltán/0000-0002-8953-920X} }